qiaamp dneasy plant mini kit catalogue no 69104  (Qiagen)


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    Name:
    DNeasy Plant Mini Kit
    Description:
    For isolation of up to 30 µg total cellular DNA from plant cells and tissues or fungi Kit contents Qiagen DNeasy Plant Mini Kit 50 preps 100mg Sample 50 to 400L Elution Volume Plant Cells Tissues Sample DNA Purification Silica Technology Spin Column Format 3 to 30g Yield Rapid Isolation of Ready to use DNA For Isolation of up to 30g Total Cellular DNA from Plant Cells Tissues or Fungi Includes 50 DNeasy Mini Spin Columns 50 QIAshredder Mini Spin Columns RNase A Buffers 2mL Collection Tubes Benefits Pure DNA free from contaminants and enzyme inhibitors Rapid isolation of ready to use DNA No organic extraction no ethanol precipitatio
    Catalog Number:
    69104
    Price:
    229
    Category:
    DNeasy Plant Mini Kit
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    Structured Review

    Qiagen qiaamp dneasy plant mini kit catalogue no 69104
    DNeasy Plant Mini Kit
    For isolation of up to 30 µg total cellular DNA from plant cells and tissues or fungi Kit contents Qiagen DNeasy Plant Mini Kit 50 preps 100mg Sample 50 to 400L Elution Volume Plant Cells Tissues Sample DNA Purification Silica Technology Spin Column Format 3 to 30g Yield Rapid Isolation of Ready to use DNA For Isolation of up to 30g Total Cellular DNA from Plant Cells Tissues or Fungi Includes 50 DNeasy Mini Spin Columns 50 QIAshredder Mini Spin Columns RNase A Buffers 2mL Collection Tubes Benefits Pure DNA free from contaminants and enzyme inhibitors Rapid isolation of ready to use DNA No organic extraction no ethanol precipitatio
    https://www.bioz.com/result/qiaamp dneasy plant mini kit catalogue no 69104/product/Qiagen
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qiaamp dneasy plant mini kit catalogue no 69104 - by Bioz Stars, 2020-01
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    Clone Assay:

    Article Title: Phagocytosis-like cell engulfment by a planctomycete bacterium
    Article Snippet: Total DNA was extracted using the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. The amplicon was purified with the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany), and was then cloned into the p-GEM T-easy vector (Promega, Tokyo, Japan).

    Centrifugation:

    Article Title: Phagocytosis-like cell engulfment by a planctomycete bacterium
    Article Snippet: Uab amorphum’ and unidentified prey bacteria in the xenic culture were collected by centrifugation. .. Total DNA was extracted using the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Amplification:

    Article Title: Unraveling the Detoxification Mechanism of 2,4-Dichlorophenol by Marine-Derived Mesophotic Symbiotic Fungi Isolated from Marine Invertebrates
    Article Snippet: Identification of Fungal Strains Genomic DNA of the strains were isolated using DNeasy Plant Mini Kit (Qiagen, Germantown, MD, USA) according to manufacturer’s instructions. .. For each sample, the ITS rDNA region was amplified with primers ITS1F (5′-CTTGGTCATTTAGAGGAAGTAA-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC).

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA). .. For both standard curves generated, amplification efficiencies were determined as described previously (Dall’Asta et al. ). qPCR was performed in ABI PRISM 7500 Detection System (Applied Biosystems, Foster City, CA, USA) as described previously (Dall’Asta et al. ).

    Article Title: A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1 [W]
    Article Snippet: Genomic DNA was isolated from approximately 100-mg quantities of ground callus tissue using the DNeasy Plant Mini kit (Qiagen). .. Sequencing of the CRE1 , TMK3 , and AHP1 promoter and coding regions was carried out on genomic DNA regions amplified with Ex-Taq DNA polymerase (Takara Mirus Bio), with BigDye Terminator version 3.1 (Applied Biosystems).

    Article Title: Phagocytosis-like cell engulfment by a planctomycete bacterium
    Article Snippet: Total DNA was extracted using the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. The amplicon was purified with the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany), and was then cloned into the p-GEM T-easy vector (Promega, Tokyo, Japan).

    Filtration:

    Article Title: Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems in Epichloë Endophytes
    Article Snippet: Hygromycin resistant colonies were grown in 250 mL sterile culture vessels with 50 mL PD broth and 100 μg/mL hygromycin B (22 °C, dark, 150 rpm, 10–12 days) and mycelia were harvested, under sterile conditions, by filtration through layers of miracloth lining a funnel and washed with 30 mL of sterile M9 phosphate buffer (1 g/L NH4 Cl, 11 g/L Na2 HPO4 .7H2 O, 3 g/l KH2 PO4 , 5 g/L NaCl). .. Washed mycelia were transferred to 15 mL sterile conical tubes with small holes in lid, lyophilised (24–48 h, Alpha 1-4LD plus) and DNA extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to manufacturers’ instructions.

    Synthesized:

    Article Title: A conserved GH17 glycosyl hydrolase from plant pathogenic Dothideomycetes releases a DAMP causing cell death in tomato
    Article Snippet: Subsequently, the DNeasy plant mini kit (Qiagen Benelux BV, Venlo, Netherlands) was used to isolate genomic DNA according to the manufacturer’s instructions. .. Total RNA isolation from fungal mycelia or C. fulvum ‐infected tomato leaves at 4, 8, 12 and 15 dpi was performed using a hybrid method described by van Esse et al. ( ). cDNA was synthesized from 5 µg isolated total RNA using a SuperScript III first‐strand kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Quantitative RT-PCR:

    Article Title: A conserved GH17 glycosyl hydrolase from plant pathogenic Dothideomycetes releases a DAMP causing cell death in tomato
    Article Snippet: Subsequently, the DNeasy plant mini kit (Qiagen Benelux BV, Venlo, Netherlands) was used to isolate genomic DNA according to the manufacturer’s instructions. .. Quantitative PCR and qRT‐PCR was performed using SensiMix SYBR Hi‐ROX mastermix (Bioline, London, UK) according to the manufacturer’s instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: A conserved GH17 glycosyl hydrolase from plant pathogenic Dothideomycetes releases a DAMP causing cell death in tomato
    Article Snippet: Subsequently, the DNeasy plant mini kit (Qiagen Benelux BV, Venlo, Netherlands) was used to isolate genomic DNA according to the manufacturer’s instructions. .. Quantitative PCR and qRT‐PCR was performed using SensiMix SYBR Hi‐ROX mastermix (Bioline, London, UK) according to the manufacturer’s instructions.

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA). .. For both standard curves generated, amplification efficiencies were determined as described previously (Dall’Asta et al. ). qPCR was performed in ABI PRISM 7500 Detection System (Applied Biosystems, Foster City, CA, USA) as described previously (Dall’Asta et al. ).

    Article Title: Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems in Epichloë Endophytes
    Article Snippet: Washed mycelia were transferred to 15 mL sterile conical tubes with small holes in lid, lyophilised (24–48 h, Alpha 1-4LD plus) and DNA extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to manufacturers’ instructions. .. PCR components and cycling conditions using the CFX Connect™ Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA): 2× FastStart SYBR Green master mix (Roche, Basel, Switzerland), 10 µM forward and reverse primers, 2 μL template DNA, sterile ddH2 0 (VT 10; 95°C 10 min, (95 °C 30 s, 60 °C 60 s, 72 °C 30 s) × 40, melt curve 60–95 °C (0.5 °C increments) 5 min. Four transgenic endophyte strains were selected for inoculation into perennial ryegrass cv.

    Incubation:

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: A volume of 100 μL of each culture dilution was added to 100 mg of crushed leaves, and this mixture was incubated for 1 h in room temperature and stored in ultra-freezer until DNA isolation. .. DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA).

    Article Title: Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems in Epichloë Endophytes
    Article Snippet: Molecular Analysis of Transformed Endophytes Individual regenerated colonies were transferred onto petri dishes containing PDA with 200 μg/mL hygromycin B selection and incubated (22 °C, dark, 10–12 days). .. Washed mycelia were transferred to 15 mL sterile conical tubes with small holes in lid, lyophilised (24–48 h, Alpha 1-4LD plus) and DNA extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to manufacturers’ instructions.

    Article Title: Taxonomy and Conservation: A Case Study from Chamaedorea alternans
    Article Snippet: Plant material was ground using liquid nitrogen, and total genomic DNA was extracted using a DNeasy Plant Mini Kit (Qiagen). .. 50 ng of genomic DNA was digested with Mse I (New England BioLabs) and Eco RI (New England BioLabs), and ligated (T4 DNA ligase; New England BioLabs) to double-stranded adaptors through incubation at 37 °C for 12–16 h. Pre-selective and selective primers were based on primer core sequences Eco RI 5′-GAG TGC GTA CCA ATT C-3′ and Mse I 5′-GAT GAG TCC TGA GTA A-3′.

    Knock-Out:

    Article Title: The Arabidopsis Plastidic Methionine Sulfoxide Reductase B Proteins. Sequence and Activity Characteristics, Comparison of the Expression with Plastidic Methionine Sulfoxide Reductase A, and Induction by Photooxidative Stress
    Article Snippet: The MsrB2 knockout mutant was obtained from the SAIL collection and provided through the ABRC as SAIL_383_G12 line ( ). .. To determine if the insertions were in the positions predicted in the mutants and to isolate plant lines homozygous for T-DNA insertion mutations, Arabidopsis genomic DNA was extracted from leaves using DNeasy Plant Mini kit (Qiagen, Valencia, CA) and two PCR procedures were used.

    Transformation Assay:

    Article Title: Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems in Epichloë Endophytes
    Article Snippet: Paragraph title: 2.3.6. Molecular Analysis of Transformed Endophytes ... Washed mycelia were transferred to 15 mL sterile conical tubes with small holes in lid, lyophilised (24–48 h, Alpha 1-4LD plus) and DNA extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to manufacturers’ instructions.

    Southern Blot:

    Article Title: A Similar Secretome Disturbance as a Hallmark of Non-pathogenic Botrytis cinerea ATMT-Mutants?
    Article Snippet: Paragraph title: Southern Blot, TAIL-PCR and Rescue-PCR Analysis ... Fungal genomic DNA was isolated using the DNeasy plant mini kit (Qiagen), digested with Eco RV or Nco I and hybridized with a hph DIG-probe using the PCR DIG Probe Synthesis Kit (Roche) and primer pair hph15/hph21 ( ).

    Ligation:

    Article Title: Taxonomy and Conservation: A Case Study from Chamaedorea alternans
    Article Snippet: Plant material was ground using liquid nitrogen, and total genomic DNA was extracted using a DNeasy Plant Mini Kit (Qiagen). .. Pre-selective primer combination Eco RI -C/ Mse I -AC and selective primer combination Eco RI -CAC/ Mse I -ACTA were used subsequent to the restriction ligation reaction.

    Serial Dilution:

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: Furthermore, H. seropedicae CFU/g leaf was estimated using a standard curve constructed with DNA isolated from a serial dilution of H. seropedicae culture mixed with crushed plant-leaf tissues as described by Stets et al. , with modifications. .. DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA).

    Infection:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: Three leaf disks from each set, healthy vs. infected, were extracted in water to confirm the presence or absence of nematodes. .. Three leaf disks were used for DNA extraction using the DNeasy Plant Mini Kit (Qiagen, Inc.), and the final three leaf disks were subjected to NaOH lysis.

    Article Title: A conserved GH17 glycosyl hydrolase from plant pathogenic Dothideomycetes releases a DAMP causing cell death in tomato
    Article Snippet: Briefly, C. fulvum mycelia obtained from PDB cultures, or C. fulvum ‐infected tomato leaves were ground in liquid nitrogen using a mortar and pestle. .. Subsequently, the DNeasy plant mini kit (Qiagen Benelux BV, Venlo, Netherlands) was used to isolate genomic DNA according to the manufacturer’s instructions.

    Generated:

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA). .. A standard curve was generated by plotting Cq versus number of H. seropedicae CFU added to each reaction tube.

    DNA Sequencing:

    Article Title: A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1 [W]
    Article Snippet: Genomic DNA was isolated from approximately 100-mg quantities of ground callus tissue using the DNeasy Plant Mini kit (Qiagen). .. The following cycling parameters were used: 96° for 2 min, followed by 28 cycles of 94° for 15 s/60° for 3 min 45 s. Sequencing reactions were purified with the CleanSEQ reaction clean-up kit (Agencourt) and analyzed by the DNA Sequencing facility at the University of Wisconsin, Madison ( ).

    Sequencing:

    Article Title: Unraveling the Detoxification Mechanism of 2,4-Dichlorophenol by Marine-Derived Mesophotic Symbiotic Fungi Isolated from Marine Invertebrates
    Article Snippet: Identification of Fungal Strains Genomic DNA of the strains were isolated using DNeasy Plant Mini Kit (Qiagen, Germantown, MD, USA) according to manufacturer’s instructions. .. Amplicons were sequenced by Sanger sequencing (Eurofins genomics, GATC Biotech, Konstanz, Germany) and the sequences were submitted to non-redundant database of the NCBI using BLASTn program (GenBank) and compared to the corresponding sequences.

    Article Title: A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1 [W]
    Article Snippet: Paragraph title: DNA Isolation and Sequencing ... Genomic DNA was isolated from approximately 100-mg quantities of ground callus tissue using the DNeasy Plant Mini kit (Qiagen).

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: Increasing increments of nematodes from 1 to 1,000 were hand picked and combined with three non-infected A. nidus leaf disks, and total DNA was extracted using the Qiagen DNeasy Plant Mini Kit. .. DNA extraction, PCR and sequencing of other Aphelenchoides spp.

    Article Title: Phagocytosis-like cell engulfment by a planctomycete bacterium
    Article Snippet: Paragraph title: DNA extraction, PCR and sequencing ... Total DNA was extracted using the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    DNA Extraction:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: .. Three leaf disks were used for DNA extraction using the DNeasy Plant Mini Kit (Qiagen, Inc.), and the final three leaf disks were subjected to NaOH lysis. .. For the NaOH extraction method, the three leaf disks were briefly ground for 5 to 10 sec in 150 μl of 0.5N NaOH using a polypropylene Pellet Pestle (Kimble/Kontes, Vineland, NJ) to disrupt the cells, after which 5 μl was transferred immediately into a sterile 1.5 ml microcentrifuge tube containing 495 μl of 100 mM Tris-HCl, pH 8.3 ( ).

    Article Title: A conserved GH17 glycosyl hydrolase from plant pathogenic Dothideomycetes releases a DAMP causing cell death in tomato
    Article Snippet: Nucleic acid methods Cladosporium fulvum genomic DNA isolation was performed as described in Ökmen et al. ( ). .. Subsequently, the DNeasy plant mini kit (Qiagen Benelux BV, Venlo, Netherlands) was used to isolate genomic DNA according to the manufacturer’s instructions.

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: A volume of 100 μL of each culture dilution was added to 100 mg of crushed leaves, and this mixture was incubated for 1 h in room temperature and stored in ultra-freezer until DNA isolation. .. DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA).

    Article Title: A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1 [W]
    Article Snippet: Paragraph title: DNA Isolation and Sequencing ... Genomic DNA was isolated from approximately 100-mg quantities of ground callus tissue using the DNeasy Plant Mini kit (Qiagen).

    Article Title: DNA Microarray-Based Detection and Identification of Fungal Pathogens in Clinical Samples from Neutropenic Patients ▿
    Article Snippet: .. DNA extraction from fungal cultures was performed ( ) with the QIAGEN DNeasy plant mini kit (QIAGEN, Hilden, Germany) as described previously. ..

    Article Title: Feasibility of Assessing the Community Composition of Prasinophytes at the Helgoland Roads Sampling Site with a DNA Microarray ▿
    Article Snippet: Paragraph title: (iii) DNA extraction. ... The template DNA was extracted from pure cultures with the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.

    Article Title: MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei: a Novel Approach Using Lineage-Specific Genes ▿
    Article Snippet: .. DNA extraction was performed by using a DNeasy plant mini kit according to the manufacturer's instructions (QIAGEN, Hilden, Germany). .. The extracted DNA was eluted in 50 μl of AE buffer (QIAGEN, Hilden, Germany), the resultant mixture was diluted 10 times, and 1 μl of the diluted extract was used for PCR.

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: Increasing increments of nematodes from 1 to 1,000 were hand picked and combined with three non-infected A. nidus leaf disks, and total DNA was extracted using the Qiagen DNeasy Plant Mini Kit. .. DNA extraction, PCR and sequencing of other Aphelenchoides spp.

    Article Title: Taxonomy and Conservation: A Case Study from Chamaedorea alternans
    Article Snippet: Paragraph title: DNA isolation and AFLP fingerprinting ... Plant material was ground using liquid nitrogen, and total genomic DNA was extracted using a DNeasy Plant Mini Kit (Qiagen).

    Article Title: Phagocytosis-like cell engulfment by a planctomycete bacterium
    Article Snippet: Paragraph title: DNA extraction, PCR and sequencing ... Total DNA was extracted using the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Sensitive Assay:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: Increasing increments of nematodes from 1 to 1,000 were hand picked and combined with three non-infected A. nidus leaf disks, and total DNA was extracted using the Qiagen DNeasy Plant Mini Kit. .. Extracted DNA from each sensitivity assay was used as template in PCR-reaction mixtures with the A. fragariae-specific primer set, AFragFl and AFragRl.

    Mutagenesis:

    Article Title: The Arabidopsis Plastidic Methionine Sulfoxide Reductase B Proteins. Sequence and Activity Characteristics, Comparison of the Expression with Plastidic Methionine Sulfoxide Reductase A, and Induction by Photooxidative Stress
    Article Snippet: The MsrB2 knockout mutant was obtained from the SAIL collection and provided through the ABRC as SAIL_383_G12 line ( ). .. To determine if the insertions were in the positions predicted in the mutants and to isolate plant lines homozygous for T-DNA insertion mutations, Arabidopsis genomic DNA was extracted from leaves using DNeasy Plant Mini kit (Qiagen, Valencia, CA) and two PCR procedures were used.

    Isolation:

    Article Title: The Arabidopsis Plastidic Methionine Sulfoxide Reductase B Proteins. Sequence and Activity Characteristics, Comparison of the Expression with Plastidic Methionine Sulfoxide Reductase A, and Induction by Photooxidative Stress
    Article Snippet: Paragraph title: Isolation of Arabidopsis Homozygous Mutants ... To determine if the insertions were in the positions predicted in the mutants and to isolate plant lines homozygous for T-DNA insertion mutations, Arabidopsis genomic DNA was extracted from leaves using DNeasy Plant Mini kit (Qiagen, Valencia, CA) and two PCR procedures were used.

    Article Title: Unraveling the Detoxification Mechanism of 2,4-Dichlorophenol by Marine-Derived Mesophotic Symbiotic Fungi Isolated from Marine Invertebrates
    Article Snippet: .. Identification of Fungal Strains Genomic DNA of the strains were isolated using DNeasy Plant Mini Kit (Qiagen, Germantown, MD, USA) according to manufacturer’s instructions. .. For each sample, the ITS rDNA region was amplified with primers ITS1F (5′-CTTGGTCATTTAGAGGAAGTAA-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC).

    Article Title: A Similar Secretome Disturbance as a Hallmark of Non-pathogenic Botrytis cinerea ATMT-Mutants?
    Article Snippet: .. Fungal genomic DNA was isolated using the DNeasy plant mini kit (Qiagen), digested with Eco RV or Nco I and hybridized with a hph DIG-probe using the PCR DIG Probe Synthesis Kit (Roche) and primer pair hph15/hph21 ( ). .. Digested DNA was transferred onto a nylon membrane and treated following manufacturer’s instructions using DIG Luminescent Detection Kit (Roche).

    Article Title: A conserved GH17 glycosyl hydrolase from plant pathogenic Dothideomycetes releases a DAMP causing cell death in tomato
    Article Snippet: Subsequently, the DNeasy plant mini kit (Qiagen Benelux BV, Venlo, Netherlands) was used to isolate genomic DNA according to the manufacturer’s instructions. .. Total RNA isolation from fungal mycelia or C. fulvum ‐infected tomato leaves at 4, 8, 12 and 15 dpi was performed using a hybrid method described by van Esse et al. ( ). cDNA was synthesized from 5 µg isolated total RNA using a SuperScript III first‐strand kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: .. DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA). .. A standard curve was generated by plotting Cq versus number of H. seropedicae CFU added to each reaction tube.

    Article Title: A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1 [W]
    Article Snippet: .. Genomic DNA was isolated from approximately 100-mg quantities of ground callus tissue using the DNeasy Plant Mini kit (Qiagen). .. Sequencing of the CRE1 , TMK3 , and AHP1 promoter and coding regions was carried out on genomic DNA regions amplified with Ex-Taq DNA polymerase (Takara Mirus Bio), with BigDye Terminator version 3.1 (Applied Biosystems).

    Negative Control:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: DNA extracted from leaf disks without the addition of a nematode was used as a negative control. .. Increasing increments of nematodes from 1 to 1,000 were hand picked and combined with three non-infected A. nidus leaf disks, and total DNA was extracted using the Qiagen DNeasy Plant Mini Kit.

    Size-exclusion Chromatography:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: Three leaf disks were used for DNA extraction using the DNeasy Plant Mini Kit (Qiagen, Inc.), and the final three leaf disks were subjected to NaOH lysis. .. For the NaOH extraction method, the three leaf disks were briefly ground for 5 to 10 sec in 150 μl of 0.5N NaOH using a polypropylene Pellet Pestle (Kimble/Kontes, Vineland, NJ) to disrupt the cells, after which 5 μl was transferred immediately into a sterile 1.5 ml microcentrifuge tube containing 495 μl of 100 mM Tris-HCl, pH 8.3 ( ).

    Purification:

    Article Title: A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture 1 [W]
    Article Snippet: Genomic DNA was isolated from approximately 100-mg quantities of ground callus tissue using the DNeasy Plant Mini kit (Qiagen). .. The following cycling parameters were used: 96° for 2 min, followed by 28 cycles of 94° for 15 s/60° for 3 min 45 s. Sequencing reactions were purified with the CleanSEQ reaction clean-up kit (Agencourt) and analyzed by the DNA Sequencing facility at the University of Wisconsin, Madison ( ).

    Article Title: Phagocytosis-like cell engulfment by a planctomycete bacterium
    Article Snippet: Total DNA was extracted using the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. The amplicon was purified with the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany), and was then cloned into the p-GEM T-easy vector (Promega, Tokyo, Japan).

    Polymerase Chain Reaction:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: Extracted DNA from each plant sample was used as template in PCR-reaction mixtures with the A. fragariae -specific primer set. .. Three leaf disks were used for DNA extraction using the DNeasy Plant Mini Kit (Qiagen, Inc.), and the final three leaf disks were subjected to NaOH lysis.

    Article Title: The Arabidopsis Plastidic Methionine Sulfoxide Reductase B Proteins. Sequence and Activity Characteristics, Comparison of the Expression with Plastidic Methionine Sulfoxide Reductase A, and Induction by Photooxidative Stress
    Article Snippet: .. To determine if the insertions were in the positions predicted in the mutants and to isolate plant lines homozygous for T-DNA insertion mutations, Arabidopsis genomic DNA was extracted from leaves using DNeasy Plant Mini kit (Qiagen, Valencia, CA) and two PCR procedures were used. ..

    Article Title: A Similar Secretome Disturbance as a Hallmark of Non-pathogenic Botrytis cinerea ATMT-Mutants?
    Article Snippet: .. Fungal genomic DNA was isolated using the DNeasy plant mini kit (Qiagen), digested with Eco RV or Nco I and hybridized with a hph DIG-probe using the PCR DIG Probe Synthesis Kit (Roche) and primer pair hph15/hph21 ( ). .. Digested DNA was transferred onto a nylon membrane and treated following manufacturer’s instructions using DIG Luminescent Detection Kit (Roche).

    Article Title: Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems in Epichloë Endophytes
    Article Snippet: Washed mycelia were transferred to 15 mL sterile conical tubes with small holes in lid, lyophilised (24–48 h, Alpha 1-4LD plus) and DNA extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to manufacturers’ instructions. .. Transformed individuals were identified by polymerase chain reaction (PCR) for the hygromycin gene (hph ; fwd 5′-tgtcgtccatcacagtttgc-3′, rev 5′-gcgccgatggtttctacaaa-3′) and/or the candidate jtmD gene fragments (jtmD (129bp); fwd 5′-cacacagcccaagattgcat-3′, rev 5′-tggaagtctatcgccactgg-3′; jtmD (432bp); fwd 5′-ggagttcagtgcatgctcag-3′, rev 5′-ggcaagaagaaaggctcacc-3′) carried by the RNA silencing vectors.

    Article Title: MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei: a Novel Approach Using Lineage-Specific Genes ▿
    Article Snippet: DNA extraction was performed by using a DNeasy plant mini kit according to the manufacturer's instructions (QIAGEN, Hilden, Germany). .. The extracted DNA was eluted in 50 μl of AE buffer (QIAGEN, Hilden, Germany), the resultant mixture was diluted 10 times, and 1 μl of the diluted extract was used for PCR.

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: Increasing increments of nematodes from 1 to 1,000 were hand picked and combined with three non-infected A. nidus leaf disks, and total DNA was extracted using the Qiagen DNeasy Plant Mini Kit. .. Extracted DNA from each sensitivity assay was used as template in PCR-reaction mixtures with the A. fragariae-specific primer set, AFragFl and AFragRl.

    Article Title: Phagocytosis-like cell engulfment by a planctomycete bacterium
    Article Snippet: Paragraph title: DNA extraction, PCR and sequencing ... Total DNA was extracted using the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Construct:

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: Furthermore, H. seropedicae CFU/g leaf was estimated using a standard curve constructed with DNA isolated from a serial dilution of H. seropedicae culture mixed with crushed plant-leaf tissues as described by Stets et al. , with modifications. .. DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA).

    Lysis:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: .. Three leaf disks were used for DNA extraction using the DNeasy Plant Mini Kit (Qiagen, Inc.), and the final three leaf disks were subjected to NaOH lysis. .. For the NaOH extraction method, the three leaf disks were briefly ground for 5 to 10 sec in 150 μl of 0.5N NaOH using a polypropylene Pellet Pestle (Kimble/Kontes, Vineland, NJ) to disrupt the cells, after which 5 μl was transferred immediately into a sterile 1.5 ml microcentrifuge tube containing 495 μl of 100 mM Tris-HCl, pH 8.3 ( ).

    Plasmid Preparation:

    Article Title: Phagocytosis-like cell engulfment by a planctomycete bacterium
    Article Snippet: Total DNA was extracted using the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. The amplicon was purified with the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany), and was then cloned into the p-GEM T-easy vector (Promega, Tokyo, Japan).

    SYBR Green Assay:

    Article Title: Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems in Epichloë Endophytes
    Article Snippet: Washed mycelia were transferred to 15 mL sterile conical tubes with small holes in lid, lyophilised (24–48 h, Alpha 1-4LD plus) and DNA extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to manufacturers’ instructions. .. PCR components and cycling conditions using the CFX Connect™ Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA): 2× FastStart SYBR Green master mix (Roche, Basel, Switzerland), 10 µM forward and reverse primers, 2 μL template DNA, sterile ddH2 0 (VT 10; 95°C 10 min, (95 °C 30 s, 60 °C 60 s, 72 °C 30 s) × 40, melt curve 60–95 °C (0.5 °C increments) 5 min. Four transgenic endophyte strains were selected for inoculation into perennial ryegrass cv.

    RNA Extraction:

    Article Title: Cross-Species Translocation of mRNA from Host Plants into the Parasitic Plant Dodder 1Cross-Species Translocation of mRNA from Host Plants into the Parasitic Plant Dodder 1 [OA]
    Article Snippet: Paragraph title: DNA and RNA Extraction ... DNA was extracted from host and dodder tissues using a DNeasy plant mini kit (Qiagen) according to the manufacturer's recommendations.

    Selection:

    Article Title: Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems in Epichloë Endophytes
    Article Snippet: Molecular Analysis of Transformed Endophytes Individual regenerated colonies were transferred onto petri dishes containing PDA with 200 μg/mL hygromycin B selection and incubated (22 °C, dark, 10–12 days). .. Washed mycelia were transferred to 15 mL sterile conical tubes with small holes in lid, lyophilised (24–48 h, Alpha 1-4LD plus) and DNA extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to manufacturers’ instructions.

    In Vitro:

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: Maize seeds (P30F53) were surface-sterilized as described above and plants were grown under axenic conditions as in vitro experiment described previously (Pereira et al. ). .. DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA).

    Transgenic Assay:

    Article Title: The Arabidopsis Plastidic Methionine Sulfoxide Reductase B Proteins. Sequence and Activity Characteristics, Comparison of the Expression with Plastidic Methionine Sulfoxide Reductase A, and Induction by Photooxidative Stress
    Article Snippet: The MsrB1 knockout mutant, corresponding to a transgenic T-DNA insertion plant, was obtained from Max Planck Institute (Germany) and termed GABI-Kat line 540H01 ( ). .. To determine if the insertions were in the positions predicted in the mutants and to isolate plant lines homozygous for T-DNA insertion mutations, Arabidopsis genomic DNA was extracted from leaves using DNeasy Plant Mini kit (Qiagen, Valencia, CA) and two PCR procedures were used.

    Article Title: Analysis of the Indole Diterpene Gene Cluster for Biosynthesis of the Epoxy-Janthitrems in Epichloë Endophytes
    Article Snippet: Washed mycelia were transferred to 15 mL sterile conical tubes with small holes in lid, lyophilised (24–48 h, Alpha 1-4LD plus) and DNA extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to manufacturers’ instructions. .. PCR components and cycling conditions using the CFX Connect™ Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA): 2× FastStart SYBR Green master mix (Roche, Basel, Switzerland), 10 µM forward and reverse primers, 2 μL template DNA, sterile ddH2 0 (VT 10; 95°C 10 min, (95 °C 30 s, 60 °C 60 s, 72 °C 30 s) × 40, melt curve 60–95 °C (0.5 °C increments) 5 min. Four transgenic endophyte strains were selected for inoculation into perennial ryegrass cv.

    Spectrophotometry:

    Article Title: Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
    Article Snippet: .. DNA was isolated from this whole mixture using DNeasy Plant Mini Kit (Qiagen) according to manufacturer’s instructions and DNA concentrations were assessed by measurement of the optical density at 260 and 280 nm on a Thermo Scientific NanoDrop 2000 spectrophotometer (Willmington, DE, USA). .. A standard curve was generated by plotting Cq versus number of H. seropedicae CFU added to each reaction tube.

    Gel Extraction:

    Article Title: Phagocytosis-like cell engulfment by a planctomycete bacterium
    Article Snippet: Total DNA was extracted using the DNeasy plant mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. The amplicon was purified with the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany), and was then cloned into the p-GEM T-easy vector (Promega, Tokyo, Japan).

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    Qiagen dneasy plant mini kit
    Dneasy Plant Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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