dna isolation kit  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    DNeasy Plant Mini Kit
    Description:
    For isolation of up to 30 µg total cellular DNA from plant cells and tissues or fungi Kit contents Qiagen DNeasy Plant Mini Kit 50 preps 100mg Sample 50 to 400L Elution Volume Plant Cells Tissues Sample DNA Purification Silica Technology Spin Column Format 3 to 30g Yield Rapid Isolation of Ready to use DNA For Isolation of up to 30g Total Cellular DNA from Plant Cells Tissues or Fungi Includes 50 DNeasy Mini Spin Columns 50 QIAshredder Mini Spin Columns RNase A Buffers 2mL Collection Tubes Benefits Pure DNA free from contaminants and enzyme inhibitors Rapid isolation of ready to use DNA No organic extraction no ethanol precipitatio
    Catalog Number:
    69104
    Price:
    229
    Category:
    DNeasy Plant Mini Kit
    Buy from Supplier


    Structured Review

    Qiagen dna isolation kit
    DNeasy Plant Mini Kit
    For isolation of up to 30 µg total cellular DNA from plant cells and tissues or fungi Kit contents Qiagen DNeasy Plant Mini Kit 50 preps 100mg Sample 50 to 400L Elution Volume Plant Cells Tissues Sample DNA Purification Silica Technology Spin Column Format 3 to 30g Yield Rapid Isolation of Ready to use DNA For Isolation of up to 30g Total Cellular DNA from Plant Cells Tissues or Fungi Includes 50 DNeasy Mini Spin Columns 50 QIAshredder Mini Spin Columns RNase A Buffers 2mL Collection Tubes Benefits Pure DNA free from contaminants and enzyme inhibitors Rapid isolation of ready to use DNA No organic extraction no ethanol precipitatio
    https://www.bioz.com/result/dna isolation kit/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna isolation kit - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis"

    Article Title: Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0231355

    Representative image of DNA extraction and PCR analysis of H6H and PMT gene isolated from EMS treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).
    Figure Legend Snippet: Representative image of DNA extraction and PCR analysis of H6H and PMT gene isolated from EMS treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).

    Techniques Used: DNA Extraction, Polymerase Chain Reaction, Isolation, Amplification

    2) Product Images from "In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes"

    Article Title: In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes

    Journal: Plants

    doi: 10.3390/plants6040052

    Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.
    Figure Legend Snippet: Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.

    Techniques Used: DNA Extraction, Modification

    Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.
    Figure Legend Snippet: Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.

    Techniques Used: DNA Extraction, Modification

    3) Product Images from "Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species"

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species

    Journal:

    doi:

    Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;
    Figure Legend Snippet: Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;

    Techniques Used: DNA Extraction, Infection, Polymerase Chain Reaction

    4) Product Images from "Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species"

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species

    Journal:

    doi:

    Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;
    Figure Legend Snippet: Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;

    Techniques Used: DNA Extraction, Infection, Polymerase Chain Reaction

    5) Product Images from "In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes"

    Article Title: In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes

    Journal: Plants

    doi: 10.3390/plants6040052

    Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.
    Figure Legend Snippet: Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.

    Techniques Used: DNA Extraction, Modification

    Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.
    Figure Legend Snippet: Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.

    Techniques Used: DNA Extraction, Modification

    6) Product Images from "Filter paper-based spin column method for cost-efficient DNA or RNA purification"

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0203011

    The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.
    Figure Legend Snippet: The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.

    Techniques Used: Purification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Gel Extraction, Plasmid Preparation

    7) Product Images from "A Transcript Profiling Approach Reveals an Abscisic Acid-Specific Glycosyltransferase (UGT73C14) Induced in Developing Fiber of Ligon lintless-2 Mutant of Cotton (Gossypium hirsutum L.)"

    Article Title: A Transcript Profiling Approach Reveals an Abscisic Acid-Specific Glycosyltransferase (UGT73C14) Induced in Developing Fiber of Ligon lintless-2 Mutant of Cotton (Gossypium hirsutum L.)

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0075268

    Copy Number Variation Assay of UGT73C14 using the single copy GhMYB25 as a reference gene. Genomic DNA was isolated from frozen leaf tissues using Qiagen DNeasy DNA extraction kit, and subjected to a 5 cycle Specific Template Amplification (STA) reaction. The STA reaction product was then subject to Taqman digital PCR following the manufacturer’s recommendations and microfluidic chips provided by Fluidigm. A ratio of UGT73C14 positive wells to GhMYB25 positive wells determined the relative copy number. To determine the copy number (y-axis values), for tetraploid lines (G. hirsutum ), the UGT73C14/GhMYB25 ratio was multiplied by four, and for diploid lines (G. raimondii, G . herbaceum and G . arboreum ), the UGT73C14/GhMYB25 ratio was muliplied by two. Error Bars represents the 95% Confidence Interval.
    Figure Legend Snippet: Copy Number Variation Assay of UGT73C14 using the single copy GhMYB25 as a reference gene. Genomic DNA was isolated from frozen leaf tissues using Qiagen DNeasy DNA extraction kit, and subjected to a 5 cycle Specific Template Amplification (STA) reaction. The STA reaction product was then subject to Taqman digital PCR following the manufacturer’s recommendations and microfluidic chips provided by Fluidigm. A ratio of UGT73C14 positive wells to GhMYB25 positive wells determined the relative copy number. To determine the copy number (y-axis values), for tetraploid lines (G. hirsutum ), the UGT73C14/GhMYB25 ratio was multiplied by four, and for diploid lines (G. raimondii, G . herbaceum and G . arboreum ), the UGT73C14/GhMYB25 ratio was muliplied by two. Error Bars represents the 95% Confidence Interval.

    Techniques Used: Isolation, DNA Extraction, Amplification, Digital PCR

    8) Product Images from "In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes"

    Article Title: In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes

    Journal: Plants

    doi: 10.3390/plants6040052

    Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.
    Figure Legend Snippet: Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.

    Techniques Used: DNA Extraction, Modification

    9) Product Images from "Hydration-dependent phase separation of a prion-like protein regulates seed germination during water stress"

    Article Title: Hydration-dependent phase separation of a prion-like protein regulates seed germination during water stress

    Journal: bioRxiv

    doi: 10.1101/2020.08.07.242172

    F L OE1 and FLOE1 expression in Arabidopsis . (A) Tissue-specific expression of FLOE1 derived from ePlant ( 37 ). (B) RT-qPCR analysis of different developmental stages shows peak expression in mature dry seeds, and a decrease in expression upon imbibition. “Dark”, “green” and “yellow” refer to the maturation stages of the siliques (from younger to older), which roughly correspond to 4-7, 8-10 and 11-13 days post-anthesis ( 22 ), and “imbibed” corresponds to seeds that were imbibed in sterile double-distilled water for 24 h. Col-0 (WT) plants were used. One-way ANOVA. **** p-value
    Figure Legend Snippet: F L OE1 and FLOE1 expression in Arabidopsis . (A) Tissue-specific expression of FLOE1 derived from ePlant ( 37 ). (B) RT-qPCR analysis of different developmental stages shows peak expression in mature dry seeds, and a decrease in expression upon imbibition. “Dark”, “green” and “yellow” refer to the maturation stages of the siliques (from younger to older), which roughly correspond to 4-7, 8-10 and 11-13 days post-anthesis ( 22 ), and “imbibed” corresponds to seeds that were imbibed in sterile double-distilled water for 24 h. Col-0 (WT) plants were used. One-way ANOVA. **** p-value

    Techniques Used: Expressing, Derivative Assay, Quantitative RT-PCR

    10) Product Images from "Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species"

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species

    Journal:

    doi:

    Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;
    Figure Legend Snippet: Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;

    Techniques Used: DNA Extraction, Infection, Polymerase Chain Reaction

    11) Product Images from "Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species"

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species

    Journal:

    doi:

    Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;
    Figure Legend Snippet: Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;

    Techniques Used: DNA Extraction, Infection, Polymerase Chain Reaction

    12) Product Images from "An Effector from the Cyst Nematode Heterodera schachtii Derepresses Host rRNA Genes by Altering Histone Acetylation [OPEN]"

    Article Title: An Effector from the Cyst Nematode Heterodera schachtii Derepresses Host rRNA Genes by Altering Histone Acetylation [OPEN]

    Journal: The Plant Cell

    doi: 10.1105/tpc.18.00570

    A Subset of VAR1 rRNA Variant Is Derepressed and VAR1:45S pre-rRNA Ratio Is Altered in the Arabidopsis 32E03-L Line. (A) Expression of subtypes of rRNA variants in roots of Arabidopsis 32E03-L line analyzed by SNP analysis. Wild-type roots were used as control. (B) Expression of subtypes of rRNA variants in Arabidopsis wild-type root segments enriched in H. schachtii -induced syncytia analyzed by SNP analysis. Wild-type plants were inoculated with H. schachtii J2s and root segments enriched in H. schachtii -induced syncytia (root+syncytium) and adjacent root segments without syncytia (root-syncytium; control) were dissected at 10 d postinoculation. In (A) and (B) , whole root or root segment cDNA was synthesized, subtypes of rRNA variants were amplified by PCR, gel-eluted, digested with Sph I, Alu I, or Msp I to detect VAR1-6645, VAR2-4302, or VAR3-7122 subtype, respectively. DNA fragments were visualized by 2.5% agarose gel electrophoresis. The experiment comprised at least two biological replicates. Similar results were obtained in the two independent experiments. Data of one representative experiment are shown. (C) Quantification of VAR1 rRNA and 45S pre-rRNA in Arabidopsis 32E03-L line (14 and 18 d old) by qPCR. Wild-type plants were used as control. (D) Quantification of rRNA VAR1 and 45S pre-rRNA in wild-type Arabidopsis root segments enriched in H. schachtii -induced syncytia by qPCR. Wild-type plants were inoculated with H. schachtii J2s and root segments enriched in H. schachtii -induced syncytia (root+syncytium) and adjacent root segments without syncytia (root-syncytium; control) were dissected at 10 d postinoculation. In (C) and (D) , whole roots or root segments cDNA was synthesized, and VAR1 and 45S pre-RNA were quantified by qPCR. ACTIN8 was amplified as reference. The experiments comprised three biological replicates, each consisting of three technical replicates. Similar results were obtained in the three independent experiments. Data of one representative experiment are shown. For (A) to (D) , plants of the tested genotypes/treatments were grown in randomized block designs. For each biological replicate, plants were sampled randomly to prepare pools for each genotype/treatment.
    Figure Legend Snippet: A Subset of VAR1 rRNA Variant Is Derepressed and VAR1:45S pre-rRNA Ratio Is Altered in the Arabidopsis 32E03-L Line. (A) Expression of subtypes of rRNA variants in roots of Arabidopsis 32E03-L line analyzed by SNP analysis. Wild-type roots were used as control. (B) Expression of subtypes of rRNA variants in Arabidopsis wild-type root segments enriched in H. schachtii -induced syncytia analyzed by SNP analysis. Wild-type plants were inoculated with H. schachtii J2s and root segments enriched in H. schachtii -induced syncytia (root+syncytium) and adjacent root segments without syncytia (root-syncytium; control) were dissected at 10 d postinoculation. In (A) and (B) , whole root or root segment cDNA was synthesized, subtypes of rRNA variants were amplified by PCR, gel-eluted, digested with Sph I, Alu I, or Msp I to detect VAR1-6645, VAR2-4302, or VAR3-7122 subtype, respectively. DNA fragments were visualized by 2.5% agarose gel electrophoresis. The experiment comprised at least two biological replicates. Similar results were obtained in the two independent experiments. Data of one representative experiment are shown. (C) Quantification of VAR1 rRNA and 45S pre-rRNA in Arabidopsis 32E03-L line (14 and 18 d old) by qPCR. Wild-type plants were used as control. (D) Quantification of rRNA VAR1 and 45S pre-rRNA in wild-type Arabidopsis root segments enriched in H. schachtii -induced syncytia by qPCR. Wild-type plants were inoculated with H. schachtii J2s and root segments enriched in H. schachtii -induced syncytia (root+syncytium) and adjacent root segments without syncytia (root-syncytium; control) were dissected at 10 d postinoculation. In (C) and (D) , whole roots or root segments cDNA was synthesized, and VAR1 and 45S pre-RNA were quantified by qPCR. ACTIN8 was amplified as reference. The experiments comprised three biological replicates, each consisting of three technical replicates. Similar results were obtained in the three independent experiments. Data of one representative experiment are shown. For (A) to (D) , plants of the tested genotypes/treatments were grown in randomized block designs. For each biological replicate, plants were sampled randomly to prepare pools for each genotype/treatment.

    Techniques Used: Variant Assay, Expressing, Synthesized, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Blocking Assay

    13) Product Images from "A modified SDS-based DNA extraction method from raw soybean"

    Article Title: A modified SDS-based DNA extraction method from raw soybean

    Journal: Bioscience Reports

    doi: 10.1042/BSR20182271

    Comparison of four different DNA extraction methods A 260/280 ratios of DNA extracted with different methods; different lowercase letters indicate significant differences amongst DNA yields in ( A ); M, 1, 2, 3, 4, and B correspond to λ DNA HindIII Marker (TIANGEN, Beijing, China), SDS-based method, CTAB method, DP305 method, DNeasy Plant Mini Kit, and negative control PCR in ( B ).
    Figure Legend Snippet: Comparison of four different DNA extraction methods A 260/280 ratios of DNA extracted with different methods; different lowercase letters indicate significant differences amongst DNA yields in ( A ); M, 1, 2, 3, 4, and B correspond to λ DNA HindIII Marker (TIANGEN, Beijing, China), SDS-based method, CTAB method, DP305 method, DNeasy Plant Mini Kit, and negative control PCR in ( B ).

    Techniques Used: DNA Extraction, Marker, Negative Control, Polymerase Chain Reaction

    14) Product Images from "Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring"

    Article Title: Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring

    Journal: Environmental Monitoring and Assessment

    doi: 10.1007/s10661-019-7840-2

    a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.
    Figure Legend Snippet: a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.

    Techniques Used: Polymerase Chain Reaction, Amplification

    15) Product Images from "Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species"

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species

    Journal:

    doi:

    Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;
    Figure Legend Snippet: Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;

    Techniques Used: DNA Extraction, Infection, Polymerase Chain Reaction

    16) Product Images from "Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis"

    Article Title: Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis

    Journal: Biotechnology Letters

    doi: 10.1007/s10529-010-0478-3

    Comparison of DNA extraction methods with 2 μg of genomic DNA and PCR products of Phytophthora and Pythium . a Kamiya and Kiguchi modified protocol; b DNeasy Plant Mini Kit (Qiagen); c 900 bp bands of ITS region; d polymorphic bands of microsatellite P74–75; e polymorphic bands of microsatellite PS36. Lanes 2–6 , P. sojae , isolates: OH 1999 1.S.1.1, OH 2000 Wood 25, OH 2000 Wood 31, OH SS05 MB 4-4-1. Lanes 7–10 , P. sansomeana , isolates: OH Mad 245, OH SS05 3B-2, OH Brown 2312. Lanes 11–15 , P. heterothallicum , isolates: OH Blan B101-32, OH Blan B101-41, OH Blan B101-31, OH Blan B408-14, OH Blan B101-22. Lane 1 : DNA ladders, a , b 0.2 μg of 1 Kb DNA ladder (Promega, USA); c , d , 0.15 μg; e 0.2 μg of 100 bp DNA ladder (Biolabs, USA)
    Figure Legend Snippet: Comparison of DNA extraction methods with 2 μg of genomic DNA and PCR products of Phytophthora and Pythium . a Kamiya and Kiguchi modified protocol; b DNeasy Plant Mini Kit (Qiagen); c 900 bp bands of ITS region; d polymorphic bands of microsatellite P74–75; e polymorphic bands of microsatellite PS36. Lanes 2–6 , P. sojae , isolates: OH 1999 1.S.1.1, OH 2000 Wood 25, OH 2000 Wood 31, OH SS05 MB 4-4-1. Lanes 7–10 , P. sansomeana , isolates: OH Mad 245, OH SS05 3B-2, OH Brown 2312. Lanes 11–15 , P. heterothallicum , isolates: OH Blan B101-32, OH Blan B101-41, OH Blan B101-31, OH Blan B408-14, OH Blan B101-22. Lane 1 : DNA ladders, a , b 0.2 μg of 1 Kb DNA ladder (Promega, USA); c , d , 0.15 μg; e 0.2 μg of 100 bp DNA ladder (Biolabs, USA)

    Techniques Used: DNA Extraction, Polymerase Chain Reaction, Modification

    17) Product Images from "Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring"

    Article Title: Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring

    Journal: Environmental Monitoring and Assessment

    doi: 10.1007/s10661-019-7840-2

    a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.
    Figure Legend Snippet: a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.

    Techniques Used: Polymerase Chain Reaction, Amplification

    18) Product Images from "Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa 1"

    Article Title: Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa 1

    Journal: Applications in Plant Sciences

    doi: 10.3732/apps.1400048

    (A) DNA yields (ng/μL) obtained via PMC isolation plotted against those obtained via DNeasy isolation. Each point represents the mean yields via PMC vs. DNeasy for an individual species. Error bars indicate standard errors associated with replicate isolations via PMC (vertical bars) and DNeasy (horizontal bars). Solid line indicates linear regression between PMC and DNeasy DNA yields; dashed line indicates line y = x. (B) DNA yields via PMC isolation vs. CTAB isolation. Points, error bars, and solid and dashed lines are as in part A, but representing yields via PMC vs. CTAB.
    Figure Legend Snippet: (A) DNA yields (ng/μL) obtained via PMC isolation plotted against those obtained via DNeasy isolation. Each point represents the mean yields via PMC vs. DNeasy for an individual species. Error bars indicate standard errors associated with replicate isolations via PMC (vertical bars) and DNeasy (horizontal bars). Solid line indicates linear regression between PMC and DNeasy DNA yields; dashed line indicates line y = x. (B) DNA yields via PMC isolation vs. CTAB isolation. Points, error bars, and solid and dashed lines are as in part A, but representing yields via PMC vs. CTAB.

    Techniques Used: Isolation

    Mean ± SE of yields of amplifiable DNA based on qPCR of matched DNA extractions obtained using PMC, DNeasy, and CTAB protocols. Species are indicated by four-letter codes determined by the first two letters of the generic name and specific epithet, respectively (refer to Table 1 ). Each protocol is marked with letters (a, b, c) indicating statistically significant differences in amplifiable yield under repeated-measures ANOVA with Holm-Bonferroni post hoc tests (see Table 2 ).
    Figure Legend Snippet: Mean ± SE of yields of amplifiable DNA based on qPCR of matched DNA extractions obtained using PMC, DNeasy, and CTAB protocols. Species are indicated by four-letter codes determined by the first two letters of the generic name and specific epithet, respectively (refer to Table 1 ). Each protocol is marked with letters (a, b, c) indicating statistically significant differences in amplifiable yield under repeated-measures ANOVA with Holm-Bonferroni post hoc tests (see Table 2 ).

    Techniques Used: Real-time Polymerase Chain Reaction

    19) Product Images from "Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis"

    Article Title: Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis

    Journal: Biotechnology Letters

    doi: 10.1007/s10529-010-0478-3

    Comparison of DNA extraction methods with 2 μg of genomic DNA and PCR products of Phytophthora and Pythium . a Kamiya and Kiguchi modified protocol; b DNeasy Plant Mini Kit (Qiagen); c 900 bp bands of ITS region; d polymorphic bands of microsatellite P74–75; e polymorphic bands of microsatellite PS36. Lanes 2–6 , P. sojae , isolates: OH 1999 1.S.1.1, OH 2000 Wood 25, OH 2000 Wood 31, OH SS05 MB 4-4-1. Lanes 7–10 , P. sansomeana , isolates: OH Mad 245, OH SS05 3B-2, OH Brown 2312. Lanes 11–15 , P. heterothallicum , isolates: OH Blan B101-32, OH Blan B101-41, OH Blan B101-31, OH Blan B408-14, OH Blan B101-22. Lane 1 : DNA ladders, a , b 0.2 μg of 1 Kb DNA ladder (Promega, USA); c , d , 0.15 μg; e 0.2 μg of 100 bp DNA ladder (Biolabs, USA)
    Figure Legend Snippet: Comparison of DNA extraction methods with 2 μg of genomic DNA and PCR products of Phytophthora and Pythium . a Kamiya and Kiguchi modified protocol; b DNeasy Plant Mini Kit (Qiagen); c 900 bp bands of ITS region; d polymorphic bands of microsatellite P74–75; e polymorphic bands of microsatellite PS36. Lanes 2–6 , P. sojae , isolates: OH 1999 1.S.1.1, OH 2000 Wood 25, OH 2000 Wood 31, OH SS05 MB 4-4-1. Lanes 7–10 , P. sansomeana , isolates: OH Mad 245, OH SS05 3B-2, OH Brown 2312. Lanes 11–15 , P. heterothallicum , isolates: OH Blan B101-32, OH Blan B101-41, OH Blan B101-31, OH Blan B408-14, OH Blan B101-22. Lane 1 : DNA ladders, a , b 0.2 μg of 1 Kb DNA ladder (Promega, USA); c , d , 0.15 μg; e 0.2 μg of 100 bp DNA ladder (Biolabs, USA)

    Techniques Used: DNA Extraction, Polymerase Chain Reaction, Modification

    20) Product Images from "Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species"

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species

    Journal:

    doi:

    Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;
    Figure Legend Snippet: Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;

    Techniques Used: DNA Extraction, Infection, Polymerase Chain Reaction

    21) Product Images from "Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papaya L.)"

    Article Title: Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papaya L.)

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-13-69

    Genomic DNA extraction from dry papaya seeds. A) Using six different techniques (Lane 1: DNeasy Plant Mini kit; 2: TRIzol; 3: Blank; 4: ‘Normal’ CTAB; 5: QIAcube; 6: Promega Maxwell 16). B) Using modified CTAB extraction procedure (Lane 1: ‘Rainbow’; 2: ‘SunUp’; 3: ‘Waimanalo’). M: 1 kb DNA ladder (Fisher Scientific).
    Figure Legend Snippet: Genomic DNA extraction from dry papaya seeds. A) Using six different techniques (Lane 1: DNeasy Plant Mini kit; 2: TRIzol; 3: Blank; 4: ‘Normal’ CTAB; 5: QIAcube; 6: Promega Maxwell 16). B) Using modified CTAB extraction procedure (Lane 1: ‘Rainbow’; 2: ‘SunUp’; 3: ‘Waimanalo’). M: 1 kb DNA ladder (Fisher Scientific).

    Techniques Used: DNA Extraction, Modification

    22) Product Images from "Filter paper-based spin column method for cost-efficient DNA or RNA purification"

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0203011

    The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.
    Figure Legend Snippet: The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.

    Techniques Used: Purification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Gel Extraction, Plasmid Preparation

    Evaluation of purification of tobacco genomic DNA and total RNA using filter paper-based spin columns with respective Qiagen kit buffers and homemade buffers. (A) Agarose gel electrophoresis for 2.5 μl tobacco genomic DNAs elution from purification experiments using Qiagen DNeasy plant mini kit buffers with Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and filter paper-based homemade spin column (Lane Q/H*), followed by tobacco genomic DNAs purified using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (B) UV spectrum curve of tobacco DNAs purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin columns with Qiagen kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve) from the same amount leaf tissue. Y-axis is UV absorbance, and X-axis is wavelength (nM). (C) Amplification plots for three duplicated qPCR reactions contain 20 ng DNA purified using Qiagen kit (Q/Q, Blue curves) or DNA purified from filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. The x-axis is PCR cycle numbers, Y-axis is the level of SYBR fluorescence, and the green line is an arbitrary threshold to determine the Cq value (the fractional cycle number at which amplification curve meet threshold level). (D) MOPS-formaldehyde denaturing agarose gel electrophoresis separated 5 μl RNA purified using Qiagen RNeasy plant mini kit buffers with a Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and homemade filter paper-based spin column (Lane Q/H*), followed total tobacco RNAs purified by using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (E) UV spectrum of tobacco total RNA purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin column with Qiagen RNeasy plant mini kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve). Y-axis is UV absorbance, and the X-axis is wavelength. (F) Amplification plots of three duplicated qRT-PCR reactions for 2.5 ng RNA purified using Qiagen kit (Q/Q, Blue curves) or RNA purified using filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. Note: * The starting material amount is 100 mg tobacco leaf tissue for experiments using a Qiagen spin column or filter paper recharged spin column, and half amount of plant sample (50 mg) used for homemade spin column purification. All DNAs or RNAs were eluted using 100 ul elution solution.
    Figure Legend Snippet: Evaluation of purification of tobacco genomic DNA and total RNA using filter paper-based spin columns with respective Qiagen kit buffers and homemade buffers. (A) Agarose gel electrophoresis for 2.5 μl tobacco genomic DNAs elution from purification experiments using Qiagen DNeasy plant mini kit buffers with Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and filter paper-based homemade spin column (Lane Q/H*), followed by tobacco genomic DNAs purified using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (B) UV spectrum curve of tobacco DNAs purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin columns with Qiagen kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve) from the same amount leaf tissue. Y-axis is UV absorbance, and X-axis is wavelength (nM). (C) Amplification plots for three duplicated qPCR reactions contain 20 ng DNA purified using Qiagen kit (Q/Q, Blue curves) or DNA purified from filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. The x-axis is PCR cycle numbers, Y-axis is the level of SYBR fluorescence, and the green line is an arbitrary threshold to determine the Cq value (the fractional cycle number at which amplification curve meet threshold level). (D) MOPS-formaldehyde denaturing agarose gel electrophoresis separated 5 μl RNA purified using Qiagen RNeasy plant mini kit buffers with a Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and homemade filter paper-based spin column (Lane Q/H*), followed total tobacco RNAs purified by using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (E) UV spectrum of tobacco total RNA purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin column with Qiagen RNeasy plant mini kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve). Y-axis is UV absorbance, and the X-axis is wavelength. (F) Amplification plots of three duplicated qRT-PCR reactions for 2.5 ng RNA purified using Qiagen kit (Q/Q, Blue curves) or RNA purified using filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. Note: * The starting material amount is 100 mg tobacco leaf tissue for experiments using a Qiagen spin column or filter paper recharged spin column, and half amount of plant sample (50 mg) used for homemade spin column purification. All DNAs or RNAs were eluted using 100 ul elution solution.

    Techniques Used: Purification, Agarose Gel Electrophoresis, Amplification, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Fluorescence, Quantitative RT-PCR

    23) Product Images from "Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring"

    Article Title: Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring

    Journal: Environmental Monitoring and Assessment

    doi: 10.1007/s10661-019-7840-2

    a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.
    Figure Legend Snippet: a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.

    Techniques Used: Polymerase Chain Reaction, Amplification

    24) Product Images from "Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring"

    Article Title: Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring

    Journal: Environmental Monitoring and Assessment

    doi: 10.1007/s10661-019-7840-2

    a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.
    Figure Legend Snippet: a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.

    Techniques Used: Polymerase Chain Reaction, Amplification

    25) Product Images from "Assessment of Passive Traps Combined with High-Throughput Sequencing To Study Airborne Fungal Communities"

    Article Title: Assessment of Passive Traps Combined with High-Throughput Sequencing To Study Airborne Fungal Communities

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02637-17

    Correlation between the total number of sequences and the total number of OTUs (a) and the Shannon index (b). Each color represents the three replicates of each of the samples. The codes indicate the technical procedures used in this paper. For type of trap: PJ, petri dish coated with a mix of petroleum jelly and Vaseline; W1, Whatman filter no. 1; W1g, Whatman filter no. 1 sprayed with a sticky layer; W3, Whatman filter no. 3; W3g, Whatman filter no. 3 sprayed with a sticky layer. For spore recovery protocol: G, grinding; R, rubbing; S, shaking. For DNA extraction kit: mnM, NucleoSpin plant II kit; mnQ, DNeasy plant minikit; mxM, NucleoSpin plant II maxikit; mxQ, DNeasy plant maxikit.
    Figure Legend Snippet: Correlation between the total number of sequences and the total number of OTUs (a) and the Shannon index (b). Each color represents the three replicates of each of the samples. The codes indicate the technical procedures used in this paper. For type of trap: PJ, petri dish coated with a mix of petroleum jelly and Vaseline; W1, Whatman filter no. 1; W1g, Whatman filter no. 1 sprayed with a sticky layer; W3, Whatman filter no. 3; W3g, Whatman filter no. 3 sprayed with a sticky layer. For spore recovery protocol: G, grinding; R, rubbing; S, shaking. For DNA extraction kit: mnM, NucleoSpin plant II kit; mnQ, DNeasy plant minikit; mxM, NucleoSpin plant II maxikit; mxQ, DNeasy plant maxikit.

    Techniques Used: DNA Extraction

    NMDS representation of all the exposed trap protocols. A protocol was the combination of the type of trap, the spore recovery procedure, the DNA extraction kit, and the ITS barcode. All samples were replicated three times. The NMDS showed acceptable values for stress (0.16) and linear fit ( R 2 = 0.89). The codes indicate the technical procedures used in this paper. For type of trap: PJ, petri dish coated with a mix of petroleum jelly and Vaseline; W1, Whatman filter no. 1; W1g, Whatman filter no. 1 sprayed with a sticky layer; W3, Whatman filter no. 3; W3g, Whatman filter no. 3 sprayed with a sticky layer. For spore recovery protocol: G, grinding; R, rubbing; S, shaking. For DNA extraction kit: mnM, NucleoSpin plant II kit; mnQ, DNeasy plant minikit; mxM, NucleoSpin plant II maxikit; mxQ, DNeasy plant maxikit.
    Figure Legend Snippet: NMDS representation of all the exposed trap protocols. A protocol was the combination of the type of trap, the spore recovery procedure, the DNA extraction kit, and the ITS barcode. All samples were replicated three times. The NMDS showed acceptable values for stress (0.16) and linear fit ( R 2 = 0.89). The codes indicate the technical procedures used in this paper. For type of trap: PJ, petri dish coated with a mix of petroleum jelly and Vaseline; W1, Whatman filter no. 1; W1g, Whatman filter no. 1 sprayed with a sticky layer; W3, Whatman filter no. 3; W3g, Whatman filter no. 3 sprayed with a sticky layer. For spore recovery protocol: G, grinding; R, rubbing; S, shaking. For DNA extraction kit: mnM, NucleoSpin plant II kit; mnQ, DNeasy plant minikit; mxM, NucleoSpin plant II maxikit; mxQ, DNeasy plant maxikit.

    Techniques Used: DNA Extraction

    26) Product Images from "A modified SDS-based DNA extraction method from raw soybean"

    Article Title: A modified SDS-based DNA extraction method from raw soybean

    Journal: Bioscience Reports

    doi: 10.1042/BSR20182271

    Comparison of four different DNA extraction methods A 260/280 ratios of DNA extracted with different methods; different lowercase letters indicate significant differences amongst DNA yields in ( A ); M, 1, 2, 3, 4, and B correspond to λ DNA HindIII Marker (TIANGEN, Beijing, China), SDS-based method, CTAB method, DP305 method, DNeasy Plant Mini Kit, and negative control PCR in ( B ).
    Figure Legend Snippet: Comparison of four different DNA extraction methods A 260/280 ratios of DNA extracted with different methods; different lowercase letters indicate significant differences amongst DNA yields in ( A ); M, 1, 2, 3, 4, and B correspond to λ DNA HindIII Marker (TIANGEN, Beijing, China), SDS-based method, CTAB method, DP305 method, DNeasy Plant Mini Kit, and negative control PCR in ( B ).

    Techniques Used: DNA Extraction, Marker, Negative Control, Polymerase Chain Reaction

    Related Articles

    DNA Extraction:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: Three leaf disks from each set, healthy vs. infected, were extracted in water to confirm the presence or absence of nematodes. .. Three leaf disks were used for DNA extraction using the DNeasy Plant Mini Kit (Qiagen, Inc.), and the final three leaf disks were subjected to NaOH lysis. .. For the NaOH extraction method, the three leaf disks were briefly ground for 5 to 10 sec in 150 μl of 0.5N NaOH using a polypropylene Pellet Pestle (Kimble/Kontes, Vineland, NJ) to disrupt the cells, after which 5 μl was transferred immediately into a sterile 1.5 ml microcentrifuge tube containing 495 μl of 100 mM Tris-HCl, pH 8.3 ( ).

    Article Title: Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis
    Article Snippet: .. Total genomic DNA was extracted from control and EMS treated samples using a DNA isolation kit (DNeasy- Plant Mini kit-Qiagen, Germany). .. The extracted DNA was quantified by Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA).

    Lysis:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species
    Article Snippet: Three leaf disks from each set, healthy vs. infected, were extracted in water to confirm the presence or absence of nematodes. .. Three leaf disks were used for DNA extraction using the DNeasy Plant Mini Kit (Qiagen, Inc.), and the final three leaf disks were subjected to NaOH lysis. .. For the NaOH extraction method, the three leaf disks were briefly ground for 5 to 10 sec in 150 μl of 0.5N NaOH using a polypropylene Pellet Pestle (Kimble/Kontes, Vineland, NJ) to disrupt the cells, after which 5 μl was transferred immediately into a sterile 1.5 ml microcentrifuge tube containing 495 μl of 100 mM Tris-HCl, pH 8.3 ( ).

    Isolation:

    Article Title: A Transcript Profiling Approach Reveals an Abscisic Acid-Specific Glycosyltransferase (UGT73C14) Induced in Developing Fiber of Ligon lintless-2 Mutant of Cotton (Gossypium hirsutum L.)
    Article Snippet: Copy Number Variation Assay Leaves from G. hirsutum (wild type and Li 2 mutant), G . raimondii , G . herbaceum and G . arboreum were collected, frozen and pulverized under liquid nitrogen, and stored at -80°C until further processing. .. Genomic DNA was isolated from the leaf tissue using Qiagen DNeasy Plant Mini Kit, catalog # 69104 (Qiagen, Germantown, Md), following the manufacturer’s instructions. .. High molecular weight DNA with no visible RNA contamination was confirmed by agarose gel electrophoresis.

    Purification:

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification
    Article Snippet: .. Plant genomic DNAs were purified using filter paper-based spin columns following the modified protocol of the Qiagen DNeasy Plant mini kit (Qiagen DNeasy plant handbook, March 2018 version) or an in-house protocol using homemade buffers described by Lemke et al [ ]. .. To lyse plant material, 400 μl of Qiagen kit AP1 buffer or homemade lysis buffer (0.5% SDS, 8% PVP-10, 250 mM NaCl, 25 mM Na-DETA, 200 mM Tris-HCl pH7.5) was added with 4 μl RNase solution (100 mg/ml, Qiagen) into a 2 ml screw cap tube containing 50 to 100 mg frozen or fresh plant material.

    Modification:

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification
    Article Snippet: .. Plant genomic DNAs were purified using filter paper-based spin columns following the modified protocol of the Qiagen DNeasy Plant mini kit (Qiagen DNeasy plant handbook, March 2018 version) or an in-house protocol using homemade buffers described by Lemke et al [ ]. .. To lyse plant material, 400 μl of Qiagen kit AP1 buffer or homemade lysis buffer (0.5% SDS, 8% PVP-10, 250 mM NaCl, 25 mM Na-DETA, 200 mM Tris-HCl pH7.5) was added with 4 μl RNase solution (100 mg/ml, Qiagen) into a 2 ml screw cap tube containing 50 to 100 mg frozen or fresh plant material.

    Article Title: In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes
    Article Snippet: In addition, we supplemented both types of lysis buffers (provided by the manufacturer) with 100 mg PVP (polyvinyl pyrrolidone, MW 10,000, Sigma-Aldrich, St. Louis, MO, USA), to aid the removal of polyphenolics from the sample, and then ground the samples for an additional three minutes using the Mini Beadbeater-24. .. The homogenates were further processed using modified steps from the standard protocols developed for the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and MoBio Power Plant Kit (MoBio, Carlsbad, CA, USA), as specified: Method # 1 (M1)—modified method based on the Qiagen DNeasy Plant Mini Kit: following sample disruption using the Mini-Beadbeater-24, samples were incubated at 75 °C for 10 min, instead of 65 °C as recommended by the manufacturer, mixing the sample thoroughly every 2 min using a vortex. .. In addition, samples were incubated for an additional 5 min, with an intense final mixing at the end of the 5 min (step 2, Quick Start protocol, Qiagen).

    Incubation:

    Article Title: In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes
    Article Snippet: In addition, we supplemented both types of lysis buffers (provided by the manufacturer) with 100 mg PVP (polyvinyl pyrrolidone, MW 10,000, Sigma-Aldrich, St. Louis, MO, USA), to aid the removal of polyphenolics from the sample, and then ground the samples for an additional three minutes using the Mini Beadbeater-24. .. The homogenates were further processed using modified steps from the standard protocols developed for the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and MoBio Power Plant Kit (MoBio, Carlsbad, CA, USA), as specified: Method # 1 (M1)—modified method based on the Qiagen DNeasy Plant Mini Kit: following sample disruption using the Mini-Beadbeater-24, samples were incubated at 75 °C for 10 min, instead of 65 °C as recommended by the manufacturer, mixing the sample thoroughly every 2 min using a vortex. .. In addition, samples were incubated for an additional 5 min, with an intense final mixing at the end of the 5 min (step 2, Quick Start protocol, Qiagen).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Qiagen dna isolation kit
    Representative image of <t>DNA</t> extraction and PCR analysis of H6H and PMT gene isolated from <t>EMS</t> treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).
    Dna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna isolation kit/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna isolation kit - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    Representative image of DNA extraction and PCR analysis of H6H and PMT gene isolated from EMS treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).

    Journal: PLoS ONE

    Article Title: Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis

    doi: 10.1371/journal.pone.0231355

    Figure Lengend Snippet: Representative image of DNA extraction and PCR analysis of H6H and PMT gene isolated from EMS treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).

    Article Snippet: Total genomic DNA was extracted from control and EMS treated samples using a DNA isolation kit (DNeasy- Plant Mini kit-Qiagen, Germany).

    Techniques: DNA Extraction, Polymerase Chain Reaction, Isolation, Amplification

    Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.

    Journal: Plants

    Article Title: In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes

    doi: 10.3390/plants6040052

    Figure Lengend Snippet: Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.

    Article Snippet: The homogenates were further processed using modified steps from the standard protocols developed for the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and MoBio Power Plant Kit (MoBio, Carlsbad, CA, USA), as specified: Method # 1 (M1)—modified method based on the Qiagen DNeasy Plant Mini Kit: following sample disruption using the Mini-Beadbeater-24, samples were incubated at 75 °C for 10 min, instead of 65 °C as recommended by the manufacturer, mixing the sample thoroughly every 2 min using a vortex.

    Techniques: DNA Extraction, Modification

    Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;

    Journal:

    Article Title: Conventional and PCR Detection of Aphelenchoides fragariae in Diverse Ornamental Host Plant Species

    doi:

    Figure Lengend Snippet: Comparison of DNA extraction methods for detection of Aphelenchoides fragariae in naturally infected Asplenium nidus (Bird's nest fern) plant tissue using species-specific PCR primers. Lane 1: healthy A. nidus extracted with Qiagen Dneasy Plant Mini Kit;

    Article Snippet: Increasing increments of nematodes from 1 to 1,000 were hand picked and combined with three non-infected A. nidus leaf disks, and total DNA was extracted using the Qiagen DNeasy Plant Mini Kit.

    Techniques: DNA Extraction, Infection, Polymerase Chain Reaction