anti ha titers (Sino Biological)

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Sino Biological anti ha titers
Anti Ha Titers, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ha titers/product/Sino Biological
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

anti ha titers - by Bioz Stars, 2021-03

93/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Increased Viral Loads and Exacerbated Innate Host Responses in Aged Macaques Infected with the 2009 Pandemic H1N1 Influenza A Virus
Article Snippet: Samples were acquired using an LSRII instrument, and the data were analyzed using FlowJo software. .. IgG antibody titers were measured by enzyme-linked immunosorbent assay (ELISA) using plates coated with recombinant CA04 HA protein (Sino Biological Inc., Beijing, China). .. ELISA plates were coated with 1 μg/ml solution antigen overnight at 4°C, washed three times with 0.05% Tween–phosphate-buffered saline (PBS), and incubated with heat-inactivated (56°C, 30 min) plasma samples in 3-fold dilutions in triplicate for 1 h. After washing three times with 0.05% Tween–PBS, horseradish peroxidase (HRP)-conjugated anti-rhesus IgG (Open Biosystems, Rockford, IL) was added for 1 h, followed by addition of o -phenylenediamine dihydrochloride (OPD) substrate (Sigma, St. Louis, MO).

Recombinant:

Incubation:

Article Title: Neutralizing Antibodies Induced by Gene-Based Hydrodynamic Injection Have a Therapeutic Effect in Lethal Influenza Infection
Article Snippet: Absorbance was measured using Spectramax M5 (Molecular Devices, CA, USA). .. Secretary IgA against HA was measured using rabbit polyclonal antibodies against mouse pIgR (Sino Biological, Boston, MA, USA), followed by incubation with HRP-conjugated goat anti-rabbit IgG (BD Pharmingen, San Diego, CA, USA). ..

HI Assay:

Article Title: Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin
Article Snippet: Serum adsorption with GH HA1 from A/Hong Kong/1/68 (H3N2) only or followed by adsorption with GH HA1 from A/Perth/16/2009 (H3N2)The convenient human serum pool (Pool) and the highest positive sample #115 in HCCIA were further analyzed by HI and traditional microneutralization (TMN) assays to explore correlations between HCCIA and existing assays. .. Many serum samples showed HI titers against the A/Aichi/2/68 (H3N2) and A/Perth/16/2009 (H3N2) viruses, indicating the presence of antibodies that bound to the HA head domain and showed hemagglutination inhibition activity. .. To focus the detection of stalk-binding antibodies, human serum samples were adsorbed with GH HA1 to remove antibodies against the HA head domain.

Activity Assay:

Staining:

Article Title: A VLP Vaccine Induces Broad-Spectrum Cross-Protective Antibody Immunity against H5N1 and H1N1 Subtypes of Influenza A Virus
Article Snippet: Plates were incubated at 37°C in a 5% CO2 incubator for 24 hr to allow plaque formation. .. Viral plaques were stained with anti-NP for H5N1 and PR8 (ab66191, Abcam, Cambridge, MA) or anti-HA for CA/07 (11055-RM05, Sino Biological Inc. Beijing, China), and the revealed plaques were scanned and counted by the AID ELISpot Reader System and EliSpot 5.0 iSpot image analyzer (AID, Strassberg, Germany). .. Microneutralization titers were calculated from the average of duplicate sample wells by extrapolating the inverse dilution of serum that produced a 50% reduction of virus by comparing the total number of plaques revealed in PBS serum and vaccination serum samples.

Enzyme-linked Immunospot:

Generated:

Article Title: Single-dose monomeric HA subunit vaccine generates full protection from influenza challenge
Article Snippet: Plate absorbance was read at 450 nm in a 96-well plate spectrophotometer (Molecular Devices). .. Anti HA titers reported in µg/ml were determined from a standard curve generated by a 3-fold serial dilution of a 100 ng/ml mouse anti-HA monoclonal antibody (Sino Biological Inc.). ..