Journal: International Journal of Molecular Sciences

Article Title: Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors

doi: 10.3390/ijms22157872

Figure Lengend Snippet: List of antibodies used for the detection of peroxisomal proteins in the parotid gland.

Article Snippet: Glyceronephosphate O-Acyltransferase(GNPAT) , Rabbit, polyclonal , 70 kDa , 1:500 , 1:5000 , Proteintech, Cat no: 14931-1-AP.

Techniques: Molecular Weight, Marker

Journal: The Journal of Clinical Investigation

Article Title: Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission

doi: 10.1172/JCI120606

Figure Lengend Snippet: (A) Ether lipid synthetic pathway. The initial steps for synthesis of ether lipids, including plasmalogens, take place in peroxisomes, generating 1-O-alkyl-glycerol-3-phosphate (AGP), a precursor for ether-linked analogs of PC and phosphatidylethanolamine. DHAP, dihydroxyacetone phosphate. (B and C) Western blot analysis suggesting that ether lipid synthetic enzymes are degraded in BAT (B) and iWAT (C) of Pex16-AKO mice (D) Targeted lipidomics analysis of mitochondrial phospholipids in BAT of WT C57 mice. PS, phosphatidylserine; PG, phosphatidylglycerol; aPC, alkyl ether PC; pPC, plasmalogen PC; PE, phosphatidylethanolamine; pPE, plasmalogen PE; n = 5. (E) Levels of various plasmalogen PE species in the mitochondrial fraction of BAT; n = 9–10. (F) Total diacyl and plasmalogen PE content in BAT mitochondria. (G) BAT SVF cells stably expressing Mito-roGFP were differentiated into adipocytes and then treated with scrambled or GNPAT shRNA and analyzed 5 days later for mitochondrial morphology using confocal microscopy. Images are representative of 3 separate experiments. Original magnification, ×60. (H) Differentiated BAT SVF cells were treated with scrambled or GNPAT shRNA. Five days later, mtDNA copy number normalized to nuclear DNA was measured by qPCR; n = 5. (I) Effect of shRNA-mediated knockdown of GNPAT on OCR was measured in BAT SVF cells using a Seahorse XF24 Extracellular Flux Analyzer; n = 8. Data are expressed as mean ± SEM and were analyzed by Student’s t test. *P < 0.05.

Article Snippet: Rabbit polyclonal antibodies against Pex16 (1:1000; catalog 14816-1-AP), Acox1 (1:1000; catalog 10957-1-AP), and GNPAT (1:1000; catalog 14931-1-AP) were from Proteintech.

Techniques: Western Blot, Stable Transfection, Expressing, shRNA, Confocal Microscopy