6500 qtrap 6500 quadrupole ion trap mass spectrometer  (SCIEX)

Journal: bioRxiv

Article Title: Glutamine is required for M1-like polarization in response to Mycobacterium tuberculosis infection

doi: 10.1101/2022.01.11.475775

Figure Lengend Snippet: Identification of highly impacted metabolic pathways during Mtb -induced M1-like polarization by widely targeted small-metabolite screening. Metabolites extracted from Mtb -infected BMDMs at 8 hrs p.i and uninfected controls were analyzed by the QTRAP 6500+ LC-MS/MS systems. Separation of M1-like macrophages from uninfected controls as scores plot from PLS-DA modeling ( A ). Highly impacted metabolic pathways in M1-like macrophages ( B ). The differential metabolites with variable importance in the projection (VIP) > 1 from the PLS-DA modeling of the two groups were subjected to pathway enrichment analysis by the Metaboanalyst (V5.0). The circle size denotes the altitude of pathway impact, and color darkness represents the extent of significance. Heatmaps of differential metabolites with VIP > 1 in highly impacted metabolic pathways ( C ). Data are shown as normalized values to the corresponding mean value of the uninfected group.

Article Snippet: Profiling of widely targeted small metabolites by QTRAP 6500+ LC-MS/MS Systems (ABSciex) BMDMs obtained by standard procedure were seeded into 6-well plates (1X106 cells per well) in DMEM with 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 4 mM glutamine, and 25 mM glucose but without pyruvate one day prior to infection.

Techniques: Infection, Liquid Chromatography with Mass Spectroscopy

Journal: Journal of Chromatographic Science

Article Title: A Sensitive and Specific ESI-Positive LC–MS/MS Method for the Quantitation of Estrogens in Human Serum in Under Three Minutes

doi: 10.1093/chromsci/bmaa104

Figure Lengend Snippet: Lower limit of quantitation plot for estradiol using the SCIEX 6500+ QTRAP.

Article Snippet: L‌LOQ The LLOQ on the SCIEX 6500+ QTRAP was determined to be 0.663 pg/mL.

Techniques: Quantitation Assay

Journal: PLoS ONE

Article Title: The procoagulant activity of tissue factor expressed on fibroblasts is increased by tissue factor-negative extracellular vesicles

doi: 10.1371/journal.pone.0240189

Figure Lengend Snippet: PS/PE species in platelets and fibroblasts. Lipids were extracted from 10 6 unstimulated fibroblasts and 2 x 10 8 freshly-isolated human platelets. PS/PE precursor scans were run using a QTRAP 6500 LC-MS/MS system as specified in materials and methods. Data are representative of three independent experiments. A) Mass spectra of PS species showing the mass-to-charge ratio (m/z) and the relative intensity (cps) of each species. B) PS intensity values from the spectra represented on the Heatmap. C) Mass spectra of PE species. D) Heat map representing PE intensity values. Molecular species refers to the total number of carbon atoms and double bonds in the two fatty acid chains of the phospholipid.

Article Snippet: Phospholipid precursor scans were run using a QTRAP 6500 LC-MS/MS system (Sciex, Framingham, MA, USA) to determine the composition of PS and PE species.

Techniques: Isolation, Liquid Chromatography with Mass Spectroscopy

Journal: eLife

Article Title: Serine ADP-ribosylation marks nucleosomes for ALC1-dependent chromatin remodeling

doi: 10.7554/eLife.71502

Figure Lengend Snippet: The recombinant PARP1:HPF1 complex installs linear poly-ADP-ribose chains at biologically relevant target sites. ( A ) Summary of LC-MS/MS analysis of enzymatic digestion products from PAR chains installed on a peptide using our technology. The spectra were produced on a Sciex QTRAP 6500+ mass spectrometer. ( B ) RP-HPLC and MS analysis of substrate peptides (H2B wild-type or S6A mutant, amino acids 1–16) and corresponding PARP1:HPF1 reaction products. RP-HPLC gradients are from 0 to 35% Solvent B (2–22 min). ( C ) RP-HPLC traces from PARG- or ARH3-treated H2B peptide ADPr reactions that were optimized for ADP-ribose chain elongation. The number of ADP-ribose units was verified by MS analysis. ( D ) Representative RP-HPLC analyses of ADPr reactions from Figure 1E containing PARP1 (1 μM), unmodified H3 substrate peptide (amino acids 1–20; 180 μM), NAD + (2 mM), and various HPF1 concentrations (indicated on corresponding trace). RP-HPLC gradients are from 0 to 35% Solvent B (2–22 min). For peak area integration values, see Supplementary Dataset.

Article Snippet: The lyophilized powder containing a mixture of the digestion products was then resuspended in water to a final concentration of 20 μg/mL and 12 μL was injected onto Phenomenex Synergi Polar-RP column (150 × 2 mm, 4 μm packing) and analyzed by LC-MS/MS using a Sciex QTRAP 6500+ mass spectrometer coupled to a Shimadzu Nexera X2 UPLC.

Techniques: Recombinant, Liquid Chromatography with Mass Spectroscopy, Produced, Mass Spectrometry, High Performance Liquid Chromatography, Mutagenesis