anti gsdmb abs  (ProSci Incorporated)


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    ProSci Incorporated anti gsdmb abs
    Anti Gsdmb Abs, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gsdmb abs/product/ProSci Incorporated
    Average 92 stars, based on 1 article reviews
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    anti gsdmb abs - by Bioz Stars, 2023-03
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    anti gsdmb abs  (ProSci Incorporated)


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  • 92

    Structured Review

    ProSci Incorporated anti gsdmb abs
    Anti Gsdmb Abs, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gsdmb abs/product/ProSci Incorporated
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gsdmb abs - by Bioz Stars, 2023-03
    92/100 stars

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    anti gsdmb abs  (ProSci Incorporated)


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    ProSci Incorporated anti gsdmb abs
    a HeLa cells or caspase 3 knockout HeLa cells were made to overexpress a gasdermin <t>(</t> <t>GSDMA,</t> <t>GSDMB,</t> GSDMC, GSDMD, DFNA5 , or DFNB59 ) or a transfected control vector ( Vector ). Those cells and parental HeLa cells were treated with staurosporine (1 μM) or TNFα (50 ng/ml) and cycloheximide (25 μg/ml) for 24 hr. The cell lysates and ApoExos were prepared, and western blotting was performed with equal amounts of protein for each gasdermin (C: cell lysates from non-treated control cells; S: cell lysates from staurosporine-treated cells; T: cell lysates from TNFα and cycloheximide–treated cells; A: lysates from the ApoExos). Arrows denote the full-length form of each gasdermin, and triangles denote the cleaved form of each gasdermin. b ApoExos from HeLa and DFNA5-overexpressing HeLa cells were coated onto aldehyde/sulfate beads and stained with anti-DFNA5 Ab (blue) or negative control Ab (red) to confirm the surface expression of DFNA5. c TEM images of the ApoExos stained with anti-DFNA5 Ab and a secondary Ab tagged with gold particles. Arrows indicate DFNA5s stained with gold particles.
    Anti Gsdmb Abs, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gsdmb abs/product/ProSci Incorporated
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gsdmb abs - by Bioz Stars, 2023-03
    92/100 stars

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    1) Product Images from "Role of Gasdermins in the Biogenesis of Apoptotic Cell–Derived Exosomes"

    Article Title: Role of Gasdermins in the Biogenesis of Apoptotic Cell–Derived Exosomes

    Journal: bioRxiv

    doi: 10.1101/2021.04.27.441709

    a HeLa cells or caspase 3 knockout HeLa cells were made to overexpress a gasdermin ( GSDMA, GSDMB, GSDMC, GSDMD, DFNA5 , or DFNB59 ) or a transfected control vector ( Vector ). Those cells and parental HeLa cells were treated with staurosporine (1 μM) or TNFα (50 ng/ml) and cycloheximide (25 μg/ml) for 24 hr. The cell lysates and ApoExos were prepared, and western blotting was performed with equal amounts of protein for each gasdermin (C: cell lysates from non-treated control cells; S: cell lysates from staurosporine-treated cells; T: cell lysates from TNFα and cycloheximide–treated cells; A: lysates from the ApoExos). Arrows denote the full-length form of each gasdermin, and triangles denote the cleaved form of each gasdermin. b ApoExos from HeLa and DFNA5-overexpressing HeLa cells were coated onto aldehyde/sulfate beads and stained with anti-DFNA5 Ab (blue) or negative control Ab (red) to confirm the surface expression of DFNA5. c TEM images of the ApoExos stained with anti-DFNA5 Ab and a secondary Ab tagged with gold particles. Arrows indicate DFNA5s stained with gold particles.
    Figure Legend Snippet: a HeLa cells or caspase 3 knockout HeLa cells were made to overexpress a gasdermin ( GSDMA, GSDMB, GSDMC, GSDMD, DFNA5 , or DFNB59 ) or a transfected control vector ( Vector ). Those cells and parental HeLa cells were treated with staurosporine (1 μM) or TNFα (50 ng/ml) and cycloheximide (25 μg/ml) for 24 hr. The cell lysates and ApoExos were prepared, and western blotting was performed with equal amounts of protein for each gasdermin (C: cell lysates from non-treated control cells; S: cell lysates from staurosporine-treated cells; T: cell lysates from TNFα and cycloheximide–treated cells; A: lysates from the ApoExos). Arrows denote the full-length form of each gasdermin, and triangles denote the cleaved form of each gasdermin. b ApoExos from HeLa and DFNA5-overexpressing HeLa cells were coated onto aldehyde/sulfate beads and stained with anti-DFNA5 Ab (blue) or negative control Ab (red) to confirm the surface expression of DFNA5. c TEM images of the ApoExos stained with anti-DFNA5 Ab and a secondary Ab tagged with gold particles. Arrows indicate DFNA5s stained with gold particles.

    Techniques Used: Knock-Out, Transfection, Plasmid Preparation, Western Blot, Staining, Negative Control, Expressing

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    ProSci Incorporated anti gsdmb abs
    Anti Gsdmb Abs, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gsdmb abs/product/ProSci Incorporated
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gsdmb abs - by Bioz Stars, 2023-03
    92/100 stars
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