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Merlin interacts with Arkadia and SKOR2 in the <t>SMAD-dependent</t> pathways in the TGFβ signaling. ( A ) Western blots of Arkadia and SKOR2 following the Co-IP with the Merlin antibody. ( B ) Western blots of Merlin and SKOR2 following the Co-IP with the Arkadia antibody. ( C ) Similar Western blot pattern of Ubiquitin following the Co-IP with the Merlin antibody with the Western blots of SKOR2 in panel A and B. ( D ) Western blots in the cytoplasmic fraction. ( E ) Western blots in the nuclear fraction. F-H . Quantification of Arkadia, SKOR2, and pSMAD2/3 in the nuclear fraction with TGFβ activation. I . The <t>SBE</t> assay indicated significantly higher response to the TGFβ activation in NF2 WT -SCs comparing to its in NF2 L64P - derived SCs
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Image Search Results


Merlin interacts with Arkadia and SKOR2 in the SMAD-dependent pathways in the TGFβ signaling. ( A ) Western blots of Arkadia and SKOR2 following the Co-IP with the Merlin antibody. ( B ) Western blots of Merlin and SKOR2 following the Co-IP with the Arkadia antibody. ( C ) Similar Western blot pattern of Ubiquitin following the Co-IP with the Merlin antibody with the Western blots of SKOR2 in panel A and B. ( D ) Western blots in the cytoplasmic fraction. ( E ) Western blots in the nuclear fraction. F-H . Quantification of Arkadia, SKOR2, and pSMAD2/3 in the nuclear fraction with TGFβ activation. I . The SBE assay indicated significantly higher response to the TGFβ activation in NF2 WT -SCs comparing to its in NF2 L64P - derived SCs

Journal: Stem Cell Research & Therapy

Article Title: Interactions among Merlin, Arkadia, and SKOR2 mediate NF2-associated human Schwann cell proliferation

doi: 10.1186/s13287-025-04281-x

Figure Lengend Snippet: Merlin interacts with Arkadia and SKOR2 in the SMAD-dependent pathways in the TGFβ signaling. ( A ) Western blots of Arkadia and SKOR2 following the Co-IP with the Merlin antibody. ( B ) Western blots of Merlin and SKOR2 following the Co-IP with the Arkadia antibody. ( C ) Similar Western blot pattern of Ubiquitin following the Co-IP with the Merlin antibody with the Western blots of SKOR2 in panel A and B. ( D ) Western blots in the cytoplasmic fraction. ( E ) Western blots in the nuclear fraction. F-H . Quantification of Arkadia, SKOR2, and pSMAD2/3 in the nuclear fraction with TGFβ activation. I . The SBE assay indicated significantly higher response to the TGFβ activation in NF2 WT -SCs comparing to its in NF2 L64P - derived SCs

Article Snippet: SBE (smad binding element) assay was performed using SBE reporter kit (BPS Bioscience) following the product general protocol.

Techniques: Western Blot, Co-Immunoprecipitation Assay, Activation Assay, Derivative Assay

Reduction of SKOR2 protein level decreases the cell proliferation in SCs. ( A ) The proposed model of interaction among Merlin, Arkadia, and SKOR2 in responding to the SMAD-dependent TGF-β signaling pathway. ( B ) Representative images of NF2 L64P SCs infected with lentivirus for 72 h. Control: empty vector. SKOR2_KO: vector carrying a shRNA fragment. ( C ) Representative Western blots. ( D ) Quantitative ratio of SKOR2/β-actin. ( E ) Significantly lower cell proliferation level in SKOR2_KO SCs than control SCs based on the BrdU ELISA assay. Scale bad = 100 μm. t-test: p < 0.05

Journal: Stem Cell Research & Therapy

Article Title: Interactions among Merlin, Arkadia, and SKOR2 mediate NF2-associated human Schwann cell proliferation

doi: 10.1186/s13287-025-04281-x

Figure Lengend Snippet: Reduction of SKOR2 protein level decreases the cell proliferation in SCs. ( A ) The proposed model of interaction among Merlin, Arkadia, and SKOR2 in responding to the SMAD-dependent TGF-β signaling pathway. ( B ) Representative images of NF2 L64P SCs infected with lentivirus for 72 h. Control: empty vector. SKOR2_KO: vector carrying a shRNA fragment. ( C ) Representative Western blots. ( D ) Quantitative ratio of SKOR2/β-actin. ( E ) Significantly lower cell proliferation level in SKOR2_KO SCs than control SCs based on the BrdU ELISA assay. Scale bad = 100 μm. t-test: p < 0.05

Article Snippet: SBE (smad binding element) assay was performed using SBE reporter kit (BPS Bioscience) following the product general protocol.

Techniques: Infection, Control, Plasmid Preparation, shRNA, Western Blot, Enzyme-linked Immunosorbent Assay