resource source identifier antibodies anti f4 80 microbeads ultrapure  (AMS Biotechnology)

Journal: Scientific Reports

Article Title: Selective Deletion of Heparan Sulfotransferase Enzyme, Ndst1, in Donor Endothelial and Myeloid Precursor Cells Significantly Decreases Acute Allograft Rejection

doi: 10.1038/s41598-018-31779-7

Figure Lengend Snippet: Immunohistological analysis of M-T7 treated WT and saline treated Ndst1 −/− donor renal allografts at 10 days post-transplant (N = 26 mice). Mean numbers of positively stained cells in 3 high power fields (HPF) are presented as bar graphs demonstrate significant decreases in CD3+ T cell infiltrates for saline treated Ndst1 −/− donors and for M-T7 treated WT donors (A) . F4/80 stained monocytes were significantly decreased in saline treated Ndst1 −/− donors but significantly increased in M-T7 treated WT donors (B) . Representative CD3+ micrographs (100X) for immunohistochemical CD3+ stained grafts, saline treated WT (C) , saline treated Ndst1 −/− (D) and M-T7 treated WT (E) . Immunohistochemical F4/80 stained graft micrographs for saline-treated WT (F) , saline treated Ndst1 −/− (G) and M-T7 treated WT (H) . Arrows indicate positively strained cells. P-value < 0.05 considered significant.

Article Snippet: Renal allografts were assessed by immunohistochemical staining for macrophage and T lymphocyte invasion and for HS with rabbit polyclonal against F4/80 (macrophages; ab100790 from Abcam at 1:50 dilution), rabbit polyclonal against CD3 (T cells; ab5690 from Abcam at 1:50 dilution) and mouse antibody to 10E4 epitope of HS (Clone F58–10E4 from AMSBio at 1:50 dilution) using ABC staining technique, respectively and as previously described , – , .

Techniques: Staining, Immunohistochemical staining