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Graphic representation of experimental details of the application of MICA-based ( A ) Luciferase assay measurement procedure and ( B ) Fluo-8AM Ca 2+ cytosolic Ca 2+ levels’ detection procedure. Prussian blue-stained images of HEK293 cells labelled with ( C ) RGD-MNPs and ( D ) TREK1-MNPs compared to ( E ) non-labelled control cells. NanoOrange protein quantification results of ( F ) RGD-MNPs and ( G ) TREK1-MNPs.

Journal: International Journal of Molecular Sciences

Article Title: Magnetic Ion Channel Activation (MICA)-Enabled Screening Assay: A Dynamic Platform for Remote Activation of Mechanosensitive Ion Channels

doi: 10.3390/ijms24043364

Figure Lengend Snippet: Graphic representation of experimental details of the application of MICA-based ( A ) Luciferase assay measurement procedure and ( B ) Fluo-8AM Ca 2+ cytosolic Ca 2+ levels’ detection procedure. Prussian blue-stained images of HEK293 cells labelled with ( C ) RGD-MNPs and ( D ) TREK1-MNPs compared to ( E ) non-labelled control cells. NanoOrange protein quantification results of ( F ) RGD-MNPs and ( G ) TREK1-MNPs.

Article Snippet: The SRE reporter-HEK293 cell line (BPS biosciences, San Diego, CA, USA) was cultured in a growth medium (MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM sodium pyruvate, 1% Penicillin/Streptomycin, and 400 µg/mL Geneticin).

Techniques: Luciferase, Staining, Control

Size-dependent Fluo-8AM response with HEK293 cells labelled with ( A ) RGD-MNPs, ( B ) TREK1-MNPs, and ( C ) concentration-dependent Fluo-8AM response with RGD-MNPs and TREK1-MNPs after MICA application for 30 s (asterisks indicate significance in results using ANOVA test (* p < 0.05)).

Journal: International Journal of Molecular Sciences

Article Title: Magnetic Ion Channel Activation (MICA)-Enabled Screening Assay: A Dynamic Platform for Remote Activation of Mechanosensitive Ion Channels

doi: 10.3390/ijms24043364

Figure Lengend Snippet: Size-dependent Fluo-8AM response with HEK293 cells labelled with ( A ) RGD-MNPs, ( B ) TREK1-MNPs, and ( C ) concentration-dependent Fluo-8AM response with RGD-MNPs and TREK1-MNPs after MICA application for 30 s (asterisks indicate significance in results using ANOVA test (* p < 0.05)).

Article Snippet: The SRE reporter-HEK293 cell line (BPS biosciences, San Diego, CA, USA) was cultured in a growth medium (MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM sodium pyruvate, 1% Penicillin/Streptomycin, and 400 µg/mL Geneticin).

Techniques: Concentration Assay

Time-dependent Fluo-8AM response with HEK293 cells labelled with ( A ) RGD-MNPs, ( B ) TREK1-MNPs, and ( C ) concentration-dependent Fluo-8AM response with time after MICA application for 30 s (asterisks indicate significance in results using ANOVA test (* p < 0.05, ** p < 0.01)).

Journal: International Journal of Molecular Sciences

Article Title: Magnetic Ion Channel Activation (MICA)-Enabled Screening Assay: A Dynamic Platform for Remote Activation of Mechanosensitive Ion Channels

doi: 10.3390/ijms24043364

Figure Lengend Snippet: Time-dependent Fluo-8AM response with HEK293 cells labelled with ( A ) RGD-MNPs, ( B ) TREK1-MNPs, and ( C ) concentration-dependent Fluo-8AM response with time after MICA application for 30 s (asterisks indicate significance in results using ANOVA test (* p < 0.05, ** p < 0.01)).

Article Snippet: The SRE reporter-HEK293 cell line (BPS biosciences, San Diego, CA, USA) was cultured in a growth medium (MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM sodium pyruvate, 1% Penicillin/Streptomycin, and 400 µg/mL Geneticin).

Techniques: Concentration Assay