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Boster Bio 6 well plates
6 Well Plates, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/6 well plates/product/Boster Bio
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
6 well plates - by Bioz Stars, 2020-09
93/100 stars

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Radio Immunoprecipitation:

Article Title: Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction
Article Snippet: .. Western blot analysis The h-MSCs were cultured in 6-well plates (5 × 105 cells/well) in expansion medium for 24 h. After incubation and stimulation, cells were washed using ice-cold PBS (Boster Biol Tech, Wuhan, China) and centrifuged at 1000 rpm for 10 min. Total proteins were isolated by lysing cells in radioimmunoprecipitation assay lysis buffer containing 1% phosphatase inhibitors (Boster Biol Tech) for 30 min on ice. .. Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, Shanghai, China) was used to extract cytoplasmic and nuclear proteins according to the manufacturer’s protocol.

Isolation:

Article Title: Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction
Article Snippet: .. Western blot analysis The h-MSCs were cultured in 6-well plates (5 × 105 cells/well) in expansion medium for 24 h. After incubation and stimulation, cells were washed using ice-cold PBS (Boster Biol Tech, Wuhan, China) and centrifuged at 1000 rpm for 10 min. Total proteins were isolated by lysing cells in radioimmunoprecipitation assay lysis buffer containing 1% phosphatase inhibitors (Boster Biol Tech) for 30 min on ice. .. Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, Shanghai, China) was used to extract cytoplasmic and nuclear proteins according to the manufacturer’s protocol.

Cell Culture:

Article Title: Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction
Article Snippet: .. Western blot analysis The h-MSCs were cultured in 6-well plates (5 × 105 cells/well) in expansion medium for 24 h. After incubation and stimulation, cells were washed using ice-cold PBS (Boster Biol Tech, Wuhan, China) and centrifuged at 1000 rpm for 10 min. Total proteins were isolated by lysing cells in radioimmunoprecipitation assay lysis buffer containing 1% phosphatase inhibitors (Boster Biol Tech) for 30 min on ice. .. Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, Shanghai, China) was used to extract cytoplasmic and nuclear proteins according to the manufacturer’s protocol.

Colony Assay:

Article Title: SPOCK1 promotes breast cancer progression via interacting with SIX1 and activating AKT/mTOR signaling pathway
Article Snippet: .. Colony formation assay BC cells were inoculated in 6-well plates for incubating about 15 d. Then fixed the cells by methanol after being washed with cold PBS (Phosphate-buffered saline solution, Boster, China) and staining with Giemsa. .. 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay 5×103 BC cells seeded and grown in 96-well plates overnight and 100 μL 50 μM EdU medium (RiboBio, Guangzhou, China) per well were cultured for 2 h. Then the cells were fixed by methanol for 30 min and washing with PBS for 5 min twice.

Incubation:

Article Title: Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction
Article Snippet: .. Western blot analysis The h-MSCs were cultured in 6-well plates (5 × 105 cells/well) in expansion medium for 24 h. After incubation and stimulation, cells were washed using ice-cold PBS (Boster Biol Tech, Wuhan, China) and centrifuged at 1000 rpm for 10 min. Total proteins were isolated by lysing cells in radioimmunoprecipitation assay lysis buffer containing 1% phosphatase inhibitors (Boster Biol Tech) for 30 min on ice. .. Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, Shanghai, China) was used to extract cytoplasmic and nuclear proteins according to the manufacturer’s protocol.

Lysis:

Article Title: Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction
Article Snippet: .. Western blot analysis The h-MSCs were cultured in 6-well plates (5 × 105 cells/well) in expansion medium for 24 h. After incubation and stimulation, cells were washed using ice-cold PBS (Boster Biol Tech, Wuhan, China) and centrifuged at 1000 rpm for 10 min. Total proteins were isolated by lysing cells in radioimmunoprecipitation assay lysis buffer containing 1% phosphatase inhibitors (Boster Biol Tech) for 30 min on ice. .. Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, Shanghai, China) was used to extract cytoplasmic and nuclear proteins according to the manufacturer’s protocol.

Western Blot:

Article Title: Electromagnetic field treatment increases purinergic receptor P2X7 expression and activates its downstream Akt/GSK3β/β-catenin axis in mesenchymal stem cells under osteogenic induction
Article Snippet: .. Western blot analysis The h-MSCs were cultured in 6-well plates (5 × 105 cells/well) in expansion medium for 24 h. After incubation and stimulation, cells were washed using ice-cold PBS (Boster Biol Tech, Wuhan, China) and centrifuged at 1000 rpm for 10 min. Total proteins were isolated by lysing cells in radioimmunoprecipitation assay lysis buffer containing 1% phosphatase inhibitors (Boster Biol Tech) for 30 min on ice. .. Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, Shanghai, China) was used to extract cytoplasmic and nuclear proteins according to the manufacturer’s protocol.

Staining:

Article Title: SPOCK1 promotes breast cancer progression via interacting with SIX1 and activating AKT/mTOR signaling pathway
Article Snippet: .. Colony formation assay BC cells were inoculated in 6-well plates for incubating about 15 d. Then fixed the cells by methanol after being washed with cold PBS (Phosphate-buffered saline solution, Boster, China) and staining with Giemsa. .. 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay 5×103 BC cells seeded and grown in 96-well plates overnight and 100 μL 50 μM EdU medium (RiboBio, Guangzhou, China) per well were cultured for 2 h. Then the cells were fixed by methanol for 30 min and washing with PBS for 5 min twice.

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