dna oligonucleotides  (Integrated DNA Technologies)


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    Name:
    DNA oligo
    Description:
    Single stranded pooled or duplexed DNA synthesized to customer specifications Sspecialized platforms with industry leading synthesis capabilities deliver the purest primers for PCR dual labelled probes for qPCR indexed adapters and fusion primers for sequencing and a variety of advanced and custom products
    Catalog Number:
    do-577595
    Price:
    None
    Category:
    Nucleic acids
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    Structured Review

    Integrated DNA Technologies dna oligonucleotides
    The CRISPR system of M. tuberculosis A. The CRISPR locus of M. tuberculosis includes genes encoding Cas6 (crRNA processing), Csm1-5 (type III-A interference complex), Csm6 (ancillary ribonuclease), Cas1 and Cas2 (Adaptation). Cas6 cleaves the CRISPR <t>RNA</t> at the base of a short hairpin to generate mature crRNA that is bound by the Csm complex. On target RNA binding, the Csm complex is expected to display three enzymatic activities: target RNA cleavage ( 1 ), <t>DNA</t> cleavage by the HD domain ( 2 ) and cOA production by the cyclase domain ( 3 ). B. Purified, recombinant CRISPR-associated proteins of M. tuberculosis . M: PageRuler Unstained (Thermo Scientific); 1: Csm1-5 interference complex; 2: Csm1-5, Csm1 D630A, D631A (Cy variant); 3: Csm1-5, Csm3 D35A (C3 variant); 4: Csm6; 5: Cas6.
    Single stranded pooled or duplexed DNA synthesized to customer specifications Sspecialized platforms with industry leading synthesis capabilities deliver the purest primers for PCR dual labelled probes for qPCR indexed adapters and fusion primers for sequencing and a variety of advanced and custom products
    https://www.bioz.com/result/dna oligonucleotides/product/Integrated DNA Technologies
    Average 99 stars, based on 213 article reviews
    Price from $9.99 to $1999.99
    dna oligonucleotides - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Cyclic oligoadenylate signalling mediates Mycobacterium tuberculosis CRISPR defence"

    Article Title: Cyclic oligoadenylate signalling mediates Mycobacterium tuberculosis CRISPR defence

    Journal: bioRxiv

    doi: 10.1101/667758

    The CRISPR system of M. tuberculosis A. The CRISPR locus of M. tuberculosis includes genes encoding Cas6 (crRNA processing), Csm1-5 (type III-A interference complex), Csm6 (ancillary ribonuclease), Cas1 and Cas2 (Adaptation). Cas6 cleaves the CRISPR RNA at the base of a short hairpin to generate mature crRNA that is bound by the Csm complex. On target RNA binding, the Csm complex is expected to display three enzymatic activities: target RNA cleavage ( 1 ), DNA cleavage by the HD domain ( 2 ) and cOA production by the cyclase domain ( 3 ). B. Purified, recombinant CRISPR-associated proteins of M. tuberculosis . M: PageRuler Unstained (Thermo Scientific); 1: Csm1-5 interference complex; 2: Csm1-5, Csm1 D630A, D631A (Cy variant); 3: Csm1-5, Csm3 D35A (C3 variant); 4: Csm6; 5: Cas6.
    Figure Legend Snippet: The CRISPR system of M. tuberculosis A. The CRISPR locus of M. tuberculosis includes genes encoding Cas6 (crRNA processing), Csm1-5 (type III-A interference complex), Csm6 (ancillary ribonuclease), Cas1 and Cas2 (Adaptation). Cas6 cleaves the CRISPR RNA at the base of a short hairpin to generate mature crRNA that is bound by the Csm complex. On target RNA binding, the Csm complex is expected to display three enzymatic activities: target RNA cleavage ( 1 ), DNA cleavage by the HD domain ( 2 ) and cOA production by the cyclase domain ( 3 ). B. Purified, recombinant CRISPR-associated proteins of M. tuberculosis . M: PageRuler Unstained (Thermo Scientific); 1: Csm1-5 interference complex; 2: Csm1-5, Csm1 D630A, D631A (Cy variant); 3: Csm1-5, Csm3 D35A (C3 variant); 4: Csm6; 5: Cas6.

    Techniques Used: CRISPR, RNA Binding Assay, Purification, Recombinant, Variant Assay

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Mechanism of HIV-1 RNA Dimerization in the Central Region of the Genome and Significance for Viral Evolution *
    Article Snippet: .. DNA oligonucleotides and the HPLC-purified RNA strand used for CD spectra analyses were purchased from Integrated DNA Technologies, Inc. (Coralville, IA). ..

    Multiplex Assay:

    Article Title: A Lysate Proteome Engineering Strategy for Enhancing Cell-Free Metabolite Production
    Article Snippet: .. 2.1 Generation and Validation of Genome Engineered Strains using MAGE All multiplex allele-specific PCR (MASC-PCR), Sanger Sequencing oligos, and recombineering oligos were created manually and ordered from IDT (Coralville, IA) with standard purification. ..

    IA:

    Article Title: Mechanism of HIV-1 RNA Dimerization in the Central Region of the Genome and Significance for Viral Evolution *
    Article Snippet: .. DNA oligonucleotides and the HPLC-purified RNA strand used for CD spectra analyses were purchased from Integrated DNA Technologies, Inc. (Coralville, IA). ..

    Article Title: Solid-phase enzyme catalysis of DNA end repair and 3′ A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA
    Article Snippet: .. DNA oligos were synthesized by Integrated DNA Technologies (Coralville, IA) and dissolved in nuclease-free water prior to use (Supplementary Table ). ..

    Article Title: RNA Polymerase II Trigger Loop Mobility
    Article Snippet: .. RNA and DNA oligomers were purchased from Integrated DNA Technologies (Coralville, IA). .. RNA oligonucleotides (16.7 pmol) were labeled with [γ-32 P]ATP (16.7 pmol) (3000 Ci/mmol, PerkinElmer Life Sciences catalog no. BLU002A) using T4 polynucleotide kinase (Promega catalog no. M4101) in transcription buffer TB(40) (20 m m Tris-HCl (pH 7.9), 40 m m KCl, 5 m m MgCl2 , 2 m m 2-mercaptoethanol).

    Synthesized:

    Article Title: Solid-phase enzyme catalysis of DNA end repair and 3′ A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA
    Article Snippet: .. DNA oligos were synthesized by Integrated DNA Technologies (Coralville, IA) and dissolved in nuclease-free water prior to use (Supplementary Table ). ..

    Purification:

    Article Title: A Lysate Proteome Engineering Strategy for Enhancing Cell-Free Metabolite Production
    Article Snippet: .. 2.1 Generation and Validation of Genome Engineered Strains using MAGE All multiplex allele-specific PCR (MASC-PCR), Sanger Sequencing oligos, and recombineering oligos were created manually and ordered from IDT (Coralville, IA) with standard purification. ..

    Polymerase Chain Reaction:

    Article Title: A Lysate Proteome Engineering Strategy for Enhancing Cell-Free Metabolite Production
    Article Snippet: .. 2.1 Generation and Validation of Genome Engineered Strains using MAGE All multiplex allele-specific PCR (MASC-PCR), Sanger Sequencing oligos, and recombineering oligos were created manually and ordered from IDT (Coralville, IA) with standard purification. ..

    other:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: DNA oligonucleotides and tracrRNA were purchased from Integrated DNA Technologies (IDT).

    Article Title: CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks
    Article Snippet: Additional files Sequences of plasmids, DNA oligonucleotides, and RNA oligonucleotides used in this work.

    Article Title: RanDeL-seq: A high-throughput method to map viral cis- and trans-acting elements
    Article Snippet: DNA oligonucleotides and synthetic dsDNA were obtained from Integrated DNA Technologies (Coralville, IA, USA).

    Sequencing:

    Article Title: A Lysate Proteome Engineering Strategy for Enhancing Cell-Free Metabolite Production
    Article Snippet: .. 2.1 Generation and Validation of Genome Engineered Strains using MAGE All multiplex allele-specific PCR (MASC-PCR), Sanger Sequencing oligos, and recombineering oligos were created manually and ordered from IDT (Coralville, IA) with standard purification. ..

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    Integrated DNA Technologies 6 fam labelled double stranded probes
    6 Fam Labelled Double Stranded Probes, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 fam labelled double stranded probes/product/Integrated DNA Technologies
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6 fam labelled double stranded probes - by Bioz Stars, 2020-09
    84/100 stars
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