dna designed aptamer sequences  (Integrated DNA Technologies)


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    Structured Review

    Integrated DNA Technologies dna designed aptamer sequences
    Two sets of <t>DNA</t> <t>aptamer</t> templates
    Dna Designed Aptamer Sequences, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 85/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna designed aptamer sequences/product/Integrated DNA Technologies
    Average 85 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    dna designed aptamer sequences - by Bioz Stars, 2020-09
    85/100 stars

    Images

    1) Product Images from "A Computationally Designed DNA Aptamer Template with Specific Binding to Phosphatidylserine"

    Article Title: A Computationally Designed DNA Aptamer Template with Specific Binding to Phosphatidylserine

    Journal: Nucleic Acid Therapeutics

    doi: 10.1089/nat.2013.0415

    Two sets of DNA aptamer templates
    Figure Legend Snippet: Two sets of DNA aptamer templates

    Techniques Used:

    Binding mode of PS–DNA aptamer, SIAp4. The surface representations of SIAp4 at 20 ns in the simulation using initial structure marked by blue in the inset of are presented. PS is plotted in the CPK (ball-and-stick) format. Red color represents
    Figure Legend Snippet: Binding mode of PS–DNA aptamer, SIAp4. The surface representations of SIAp4 at 20 ns in the simulation using initial structure marked by blue in the inset of are presented. PS is plotted in the CPK (ball-and-stick) format. Red color represents

    Techniques Used: Binding Assay

    Related Articles

    Clone Assay:

    Article Title: Neuronal Sirt3 Protects against Excitotoxic Injury in Mouse Cortical Neuron Culture
    Article Snippet: .. The DNA sequences of Sirt3 primers with restriction enzyme sites for cloning were as follows - forward primer with EcoRI site ; 5′-CCG GAATTC CGGATGGTGGGGGCCGGCATC-3′ , reverse primer with XmaI site ; 5′-CCC CCCGGG GGGTTATCTGTCCTGTCCATCCAGCTT-3′ (Integrated DNA technologies). ..

    Sequencing:

    Article Title: MUC1 Aptamer Targeted SERS Nanoprobes
    Article Snippet: .. This strategy allowed us to functionalize the SERS NPs essentially with any DNA sequence with a biotin modification, typically purchased from a commercial source (IDT DNA Inc.). .. We characterized the SERS NPs using a transmission electron microscope (TEM) and dynamic light scattering (DLS).

    Article Title: A Continuous Fluorometric Assay for the Assessment of MazF Ribonuclease Activity
    Article Snippet: .. Primers, unlabeled oligonucleotides, oligonucleotides of the sequence 5′-AAGTCrGACATCAG-3′ labeled with 6-carboxyfluorescein (6-FAM) on the 5′-end and with Black Hole Quencher 1 (BHQ1) on the 3′-end, and the corresponding oligonucleotide cleavage fragments, 6-FAM-labeled 5′-AAGTCG-3′ and BHQ1-labeled 5′-ACATCAG-3′, were purchased from Integrated DNA Technologies (Coralville, IA). .. Black, tissue culture treated 384-well plates were purchased from Matrix Technologies (Hudson, NH).

    Synthesized:

    Article Title: Genetic Analysis of the Structure and Function of 7SK Small Nuclear Ribonucleoprotein (snRNP) in Cells *
    Article Snippet: .. The cDNAs of the RNA sequences from 7SK snRNA and their negative strand DNAs were chemically synthesized (IDTDNA) and annealed to form double-strand DNA inserts. ..

    Article Title: In vitro quantification of specific microRNA using molecular beacons
    Article Snippet: .. Synthetic miRNAs used for beacon hybridization To assess the sensitivity and specificity of the molecular beacons, the RNA sequences of mature miR-21, miR-155, miR-24, miR-27b and miR-27a were synthesized by Integrated DNA Technologies. .. In addition to the mature miR-21 (22 nt), pre-miR-21 (72 nt) was synthesized and used for miR-21 molecular beacon hybridization assays.

    Article Title: Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis
    Article Snippet: .. PCR primers were designed based on the known DNA sequences and relevant restriction enzymes (RE) were introduced via primers when needed (Table ) which were synthesized by Integrated DNA Technologies (Singapore). .. DNA fragments were purified using GeneJET PCR purification Kit and GeneJET Gel Extraction Kit (Thermofisher, USA).

    Nuclear Magnetic Resonance:

    Article Title: Structure of the Yeast SR protein Npl3 and Interaction with mRNA 3?-End Processing Signals
    Article Snippet: .. RNA sequences used for NMR studies were purchased as PAGE-purified oligonucleotides from IDT DNA. ..

    Labeling:

    Article Title: A Continuous Fluorometric Assay for the Assessment of MazF Ribonuclease Activity
    Article Snippet: .. Primers, unlabeled oligonucleotides, oligonucleotides of the sequence 5′-AAGTCrGACATCAG-3′ labeled with 6-carboxyfluorescein (6-FAM) on the 5′-end and with Black Hole Quencher 1 (BHQ1) on the 3′-end, and the corresponding oligonucleotide cleavage fragments, 6-FAM-labeled 5′-AAGTCG-3′ and BHQ1-labeled 5′-ACATCAG-3′, were purchased from Integrated DNA Technologies (Coralville, IA). .. Black, tissue culture treated 384-well plates were purchased from Matrix Technologies (Hudson, NH).

    Modification:

    Article Title: MUC1 Aptamer Targeted SERS Nanoprobes
    Article Snippet: .. This strategy allowed us to functionalize the SERS NPs essentially with any DNA sequence with a biotin modification, typically purchased from a commercial source (IDT DNA Inc.). .. We characterized the SERS NPs using a transmission electron microscope (TEM) and dynamic light scattering (DLS).

    Generated:

    Article Title: RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes
    Article Snippet: .. All nucleotide oligomers used in this study were generated from the specific DNA sequences of the L. monocytogenes type strain EGDe (NCBI) type strain by Integrated DNA technology (IDT). .. PCR products were examined by agarose gel electrophoresis and purified using a Wizard SV Gel and PCR clean-up kit (Promega), and submitted to the Department of Biochemistry and Molecular Biology Recombinant DNA and Protein core facility (Oklahoma State University, Stillwater, OK) for sequence identification with a ABI 3730 DNA analyzer.

    Polymerase Chain Reaction:

    Article Title: Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis
    Article Snippet: .. PCR primers were designed based on the known DNA sequences and relevant restriction enzymes (RE) were introduced via primers when needed (Table ) which were synthesized by Integrated DNA Technologies (Singapore). .. DNA fragments were purified using GeneJET PCR purification Kit and GeneJET Gel Extraction Kit (Thermofisher, USA).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Structure of the Yeast SR protein Npl3 and Interaction with mRNA 3?-End Processing Signals
    Article Snippet: .. RNA sequences used for NMR studies were purchased as PAGE-purified oligonucleotides from IDT DNA. ..

    IA:

    Article Title: A Continuous Fluorometric Assay for the Assessment of MazF Ribonuclease Activity
    Article Snippet: .. Primers, unlabeled oligonucleotides, oligonucleotides of the sequence 5′-AAGTCrGACATCAG-3′ labeled with 6-carboxyfluorescein (6-FAM) on the 5′-end and with Black Hole Quencher 1 (BHQ1) on the 3′-end, and the corresponding oligonucleotide cleavage fragments, 6-FAM-labeled 5′-AAGTCG-3′ and BHQ1-labeled 5′-ACATCAG-3′, were purchased from Integrated DNA Technologies (Coralville, IA). .. Black, tissue culture treated 384-well plates were purchased from Matrix Technologies (Hudson, NH).

    Hybridization:

    Article Title: In vitro quantification of specific microRNA using molecular beacons
    Article Snippet: .. Synthetic miRNAs used for beacon hybridization To assess the sensitivity and specificity of the molecular beacons, the RNA sequences of mature miR-21, miR-155, miR-24, miR-27b and miR-27a were synthesized by Integrated DNA Technologies. .. In addition to the mature miR-21 (22 nt), pre-miR-21 (72 nt) was synthesized and used for miR-21 molecular beacon hybridization assays.

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