qiaamp minelute virus spin kit  (Qiagen)


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    Name:
    QIAamp MinElute Virus Spin Kit
    Description:
    For simultaneous purification of viral DNA and RNA from plasma serum and cell free body fluids Kit contents Qiagen QIAamp MinElute Virus Spin Kit 50 preps 200L Sample 20 to 150L Elution Volume Serum Plasma Sample Viral DNA and RNA Purification Silica Technology Manual Processing MinElute Columns Format For Simultaneous Purification of Viral DNA and RNA from Plasma Serum and Cell free Body Fluids Ideal for PCR Real time PCR Application Includes 50 QIAamp MinElute Columns Qiagen Protease Carrier RNA Buffers 2mL Collection Tubes Benefits Rapid purification of high quality viral DNA and RNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibito
    Catalog Number:
    57704
    Price:
    278
    Category:
    QIAamp MinElute Virus Spin Kit
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    Structured Review

    Qiagen qiaamp minelute virus spin kit
    QIAamp MinElute Virus Spin Kit
    For simultaneous purification of viral DNA and RNA from plasma serum and cell free body fluids Kit contents Qiagen QIAamp MinElute Virus Spin Kit 50 preps 200L Sample 20 to 150L Elution Volume Serum Plasma Sample Viral DNA and RNA Purification Silica Technology Manual Processing MinElute Columns Format For Simultaneous Purification of Viral DNA and RNA from Plasma Serum and Cell free Body Fluids Ideal for PCR Real time PCR Application Includes 50 QIAamp MinElute Columns Qiagen Protease Carrier RNA Buffers 2mL Collection Tubes Benefits Rapid purification of high quality viral DNA and RNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibito
    https://www.bioz.com/result/qiaamp minelute virus spin kit/product/Qiagen
    Average 90 stars, based on 1326 article reviews
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    qiaamp minelute virus spin kit - by Bioz Stars, 2020-01
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    Related Articles

    Diagnostic Assay:

    Article Title: Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
    Article Snippet: Supernatant from fibroblasts infected with Toledo isolate passage 7 (P7) was used to extract viral DNA using the QIAamp MinElute virus spin kit (Qiagen). .. Two microliters of viral DNA was amplified with Taq 5× Master Mix (New England BioLabs [NEB]) using the following primers (indicated in the parentheses) for the regions and diagnostic PCR indicated: UL127 (5′-ATGTGCCAGCTTGATGTCGC-3′) and UL130 (5′-CGCCAAGATTTTTGGAGCGCAC-3′) (PCR-1); UL127 (5′-ATGTGCCAGCTTGATGTCGC-3′) and UL133 (5′-GGTTGTGAACTCACCGTCGG-3′) (PCR-2); UL133 (5′-GGTTGTGAACTCACCGTCGG-3′) and UL148 (5′-CGAGGCAGAACATCTCAACC-3′) (PCR-3); UL128 (5′-GAGGGCCTTACAGCCTATGG-3′) and UL148 (5′-CGAGGCAGAACATCTCAACC-3′) (PCR-4).

    Centrifugation:

    Article Title: Mauritian Cynomolgus Macaques Share Two Exceptionally Common Major Histocompatibility Complex Class I Alleles That Restrict Simian Immunodeficiency Virus-Specific CD8+ T Cells ▿ T Cells ▿ †
    Article Snippet: Cell-free plasma was obtained from EDTA anticoagulated whole blood by using Ficoll-Paque Plus (GE Healthcare Bioscience) and density centrifugation. .. Viral RNA in the plasma was isolated by using the QIAamp MinElute virus spin kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.

    Amplification:

    Article Title: Identification and Genome Characterization of the First Sicinivirus Isolate from Chickens in Mainland China by Using Viral Metagenomics
    Article Snippet: Viral nucleic acids were then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, Germany) according to the manufacturer’s instructions. .. Viral nucleic acid libraries were then constructed by sequence-independent RT-PCR amplification as previously described [ , ].

    Article Title: Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
    Article Snippet: Supernatant from fibroblasts infected with Toledo isolate passage 7 (P7) was used to extract viral DNA using the QIAamp MinElute virus spin kit (Qiagen). .. Two microliters of viral DNA was amplified with Taq 5× Master Mix (New England BioLabs [NEB]) using the following primers (indicated in the parentheses) for the regions and diagnostic PCR indicated: UL127 (5′-ATGTGCCAGCTTGATGTCGC-3′) and UL130 (5′-CGCCAAGATTTTTGGAGCGCAC-3′) (PCR-1); UL127 (5′-ATGTGCCAGCTTGATGTCGC-3′) and UL133 (5′-GGTTGTGAACTCACCGTCGG-3′) (PCR-2); UL133 (5′-GGTTGTGAACTCACCGTCGG-3′) and UL148 (5′-CGAGGCAGAACATCTCAACC-3′) (PCR-3); UL128 (5′-GAGGGCCTTACAGCCTATGG-3′) and UL148 (5′-CGAGGCAGAACATCTCAACC-3′) (PCR-4).

    Article Title: Phage and Nucleocytoplasmic Large Viral Sequences Dominate Coral Viromes from the Arabian Gulf
    Article Snippet: Paragraph title: Viral DNA Extraction, Amplification and Sequencing ... The viral DNA was then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, United States) by following the manufacturer’s protocol.

    Article Title: Molecular Epidemiologic Trends of Diarrhea‐Causing Virus Infection From Clinical Specimens in Cheonan, Korea, in 2010–2012
    Article Snippet: Distilled water (1 mL) was added to the stool specimen, and RNA was extracted from a 200 μL sample using a QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany). cDNA was synthesized from the extracted RNA using a RevertAid First Strand cDNA synthesis kit (Fermentas, Ontario, Canada). cDNA was synthesized in a 20 μL reaction containing 1 μL of random hexamer, 8 μL of total RNA, 1 μL of reverse transcriptase, 1 μL of RNase inhibitor, 2 μL of dNTP, 4 μL of 5× reverse transcription buffer, and 3 μL of distilled water at 37°C for 90 min. .. The mPCR products were electrophoresed in a 2% agarose gel with ethidium bromide for 30 min at 100–150 V. The agarose gel was rinsed with distilled water, amplification was assessed by visualizing the gel on a UV transilluminator, and the results were analyzed after a photograph was taken (Fig. ).

    Article Title: Complete Genome Sequence of a Rodent Torque Teno Virus in Hainan Island, China
    Article Snippet: .. Viral DNA was extracted from the liver tissue with the QIAamp MinElute virus spin kit (Qiagen), and sequence-independent amplification of viral nucleic acids was performed as described previously ( ). .. The amplicons in the 250 to 500-bp range were purified with a gel extraction kit (Tiangen).

    Article Title: Invader Plus Method Detects Herpes Simplex Virus in Cerebrospinal Fluid and Simultaneously Differentiates Types 1 and 2
    Article Snippet: Nucleic acid from 200 μl of each CSF specimen was extracted by using a QIAamp MinElute virus spin kit (QIAGEN Inc.), according to the manufacturer's instructions ( ). .. The extracted DNAs were resuspended in 50 μl of water, and 15 μl of each specimen extract was used for amplification.

    Article Title: Mauritian Cynomolgus Macaques Share Two Exceptionally Common Major Histocompatibility Complex Class I Alleles That Restrict Simian Immunodeficiency Virus-Specific CD8+ T Cells ▿ T Cells ▿ †
    Article Snippet: Viral RNA in the plasma was isolated by using the QIAamp MinElute virus spin kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. .. Viral RNA was reverse transcribed and amplified by using the One-Step reverse transcription-PCR kit (Qiagen), generating amplicons spanning the epitopes of interest from the SIVmac239 open reading frame.

    Article Title: A Recombinant Subunit Based Zika Virus Vaccine Is Efficacious in Non-human Primates
    Article Snippet: For the RT PCR (conducted on NHP samples), RNA was isolated from 200 μL plasma using the QIAamp MinElute Virus spin kit (Qiagen, Frederick, MD). .. Extracted RNA was used for amplification using the SensiFAST Probe Lo-ROX One-Step Kit (Bioline BIO-78005, Taunton, MA) on a 7,500 Real-Time PCR system (Applied Biosystems, Foster City, CA).

    Article Title: Subclinical Infection of Macaques and Baboons with A Baboon Simarterivirus
    Article Snippet: For each animal, viral RNA was isolated from approximately 200 µL of plasma using the Qiagen QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany), omitting carrier RNA. cDNA synthesis and PCR were performed using random hexamers (for the inoculum) or with seven primer sets (for the remaining animals, see ) to amplify the entire SWBV genome in overlapping amplicons with the Superscript III One-Step RT-PCR kit with Platinum Taq Polymerase (Invitrogen, Carlsbad, CA, USA). .. All amplicons from a single sample were pooled to achieve 1 ng of total amplified DNA in 5 µL.

    Positive Control:

    Article Title: Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
    Article Snippet: Supernatant from fibroblasts infected with Toledo isolate passage 7 (P7) was used to extract viral DNA using the QIAamp MinElute virus spin kit (Qiagen). .. HCMV TR DNA was extracted with the same kit as a positive control.

    Synthesized:

    Article Title: P2Y6 Receptor-Mediated Spinal Microglial Activation in Neuropathic Pain
    Article Snippet: The primer sequences of target genes and internal reference gene β -actin were designed using Primer Premier 5.0 software and then synthesized by Sangon Biotech (Shanghai). .. Total RNA was reverse transcribed into complementary DNA (cDNA) according to the instruction of QIAamp® MinElute® Virus Spin kit (Qiagen Inc USA).

    Article Title: Molecular Epidemiologic Trends of Diarrhea‐Causing Virus Infection From Clinical Specimens in Cheonan, Korea, in 2010–2012
    Article Snippet: .. Distilled water (1 mL) was added to the stool specimen, and RNA was extracted from a 200 μL sample using a QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany). cDNA was synthesized from the extracted RNA using a RevertAid First Strand cDNA synthesis kit (Fermentas, Ontario, Canada). cDNA was synthesized in a 20 μL reaction containing 1 μL of random hexamer, 8 μL of total RNA, 1 μL of reverse transcriptase, 1 μL of RNase inhibitor, 2 μL of dNTP, 4 μL of 5× reverse transcription buffer, and 3 μL of distilled water at 37°C for 90 min. ..

    Construct:

    Article Title: Identification and Genome Characterization of the First Sicinivirus Isolate from Chickens in Mainland China by Using Viral Metagenomics
    Article Snippet: Viral nucleic acids were then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, Germany) according to the manufacturer’s instructions. .. Viral nucleic acid libraries were then constructed by sequence-independent RT-PCR amplification as previously described [ , ].

    Enzyme-linked Immunosorbent Assay:

    Article Title: Safety and efficacy of anti-PD-L1 therapy in the woodchuck model of HBV infection
    Article Snippet: Circulating viral loads were determined by qPCR as previously described [ ], or alternatively by extraction of viral DNA using the QIAamp MinElute Virus Spin Kit (Qiagen) followed by qPCR with primers WHV-SL-F: 5’-CCTCGCAGGAGAAGATCTCAA-3’ , WHV-SL-R: 5’- GCAGTTGGCAGATGGAGATTG-3’ , and minor groove binding probe WHV-SL-MGB: 5’-6FAM-ACCGCGTCGCAGAC-3’ , within the core/polymerase overlapping region of the WHV genome. .. WHV precore eAg was measured using a commercially available HBeAg ELISA Kit (Autobio).

    Random Hexamer Labeling:

    Article Title: Molecular Epidemiologic Trends of Diarrhea‐Causing Virus Infection From Clinical Specimens in Cheonan, Korea, in 2010–2012
    Article Snippet: .. Distilled water (1 mL) was added to the stool specimen, and RNA was extracted from a 200 μL sample using a QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany). cDNA was synthesized from the extracted RNA using a RevertAid First Strand cDNA synthesis kit (Fermentas, Ontario, Canada). cDNA was synthesized in a 20 μL reaction containing 1 μL of random hexamer, 8 μL of total RNA, 1 μL of reverse transcriptase, 1 μL of RNase inhibitor, 2 μL of dNTP, 4 μL of 5× reverse transcription buffer, and 3 μL of distilled water at 37°C for 90 min. ..

    Article Title: Subclinical Infection of Macaques and Baboons with A Baboon Simarterivirus
    Article Snippet: For each animal, viral RNA was isolated from approximately 200 µL of plasma using the Qiagen QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany), omitting carrier RNA. cDNA synthesis and PCR were performed using random hexamers (for the inoculum) or with seven primer sets (for the remaining animals, see ) to amplify the entire SWBV genome in overlapping amplicons with the Superscript III One-Step RT-PCR kit with Platinum Taq Polymerase (Invitrogen, Carlsbad, CA, USA). .. Pooled amplicons (or total cDNA generated from random-hexamer amplification) were fragmented, and sequencing adaptors were added using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA).

    Infection:

    Article Title: Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
    Article Snippet: .. Supernatant from fibroblasts infected with Toledo isolate passage 7 (P7) was used to extract viral DNA using the QIAamp MinElute virus spin kit (Qiagen). .. HCMV TR DNA was extracted with the same kit as a positive control.

    Article Title: Human cytomegalovirus microRNAs are carried by virions and dense bodies and are delivered to target cells
    Article Snippet: DNA and RNA isolation from virions, DBs and cells Dialysed virions, DBs and mock samples were treated with micrococcal nuclease (2000 Kunitz units ml−1 ; Thermo Scientific) in the presence of 5 mM calcium chloride at 37 °C for 2 h. DNA was isolated with the QIAamp MinElute Virus Spin kit (Qiagen) without carrier RNA. .. The DNA and RNA were also isolated from infected cells at 5 days p.i. (m.o.i.

    Expressing:

    Article Title: P2Y6 Receptor-Mediated Spinal Microglial Activation in Neuropathic Pain
    Article Snippet: Total RNA was reverse transcribed into complementary DNA (cDNA) according to the instruction of QIAamp® MinElute® Virus Spin kit (Qiagen Inc USA). .. All values were normalized to β -actin expression for the loading control and then expressed as fold change with respect to mean controls values in the same run.

    Cell Culture:

    Article Title: Optimizing PCR Detection of Zika Virus from Various Body Fluids
    Article Snippet: AVE buffer, the elution buffer from the QIAamp MinElute Virus Spin Kit (QIAGEN, Valencia, CA), was used as the spiking reference control buffer to generate expected cycle threshold (Ct) values. .. The aliquots were spiked in triplicates with 10 µL of viral cell culture supernatant containing three levels of viral load, and a single aliquot was left unspiked to confirm ZIKV-negative status of the samples from the donors.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification and Genome Characterization of the First Sicinivirus Isolate from Chickens in Mainland China by Using Viral Metagenomics
    Article Snippet: Viral nucleic acids were then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, Germany) according to the manufacturer’s instructions. .. Viral nucleic acid libraries were then constructed by sequence-independent RT-PCR amplification as previously described [ , ].

    Article Title: Clinical, Virologic, and Immunologic Characteristics of Zika Virus Infection in a Cohort of US Patients: Prolonged RNA Detection in Whole Blood
    Article Snippet: RNA was extracted using the QIAamp MinElute Virus Spin kit (Qiagen) following the original manufacturer protocol (serum) or with optimizations. .. ZIKV RNA in the samples was detected by reverse transcription polymerase chain reaction (RT-PCR) using TaqMan Fast Virus 1-Step Master Mix on a ViiA 7 RT- PCR System (ThermoFisher Scientific, Waltham, MA) with a previously described primers/probe set [ ].

    Article Title: P2Y6 Receptor-Mediated Spinal Microglial Activation in Neuropathic Pain
    Article Snippet: Paragraph title: 2.7. Reverse Transcription Polymerase Chain Reaction (RT-PCR) ... Total RNA was reverse transcribed into complementary DNA (cDNA) according to the instruction of QIAamp® MinElute® Virus Spin kit (Qiagen Inc USA).

    Article Title: Molecular Epidemiologic Trends of Diarrhea‐Causing Virus Infection From Clinical Specimens in Cheonan, Korea, in 2010–2012
    Article Snippet: Paragraph title: Multiplex RT‐PCR Analysis ... Distilled water (1 mL) was added to the stool specimen, and RNA was extracted from a 200 μL sample using a QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany). cDNA was synthesized from the extracted RNA using a RevertAid First Strand cDNA synthesis kit (Fermentas, Ontario, Canada). cDNA was synthesized in a 20 μL reaction containing 1 μL of random hexamer, 8 μL of total RNA, 1 μL of reverse transcriptase, 1 μL of RNase inhibitor, 2 μL of dNTP, 4 μL of 5× reverse transcription buffer, and 3 μL of distilled water at 37°C for 90 min.

    Article Title: A Recombinant Subunit Based Zika Virus Vaccine Is Efficacious in Non-human Primates
    Article Snippet: .. For the RT PCR (conducted on NHP samples), RNA was isolated from 200 μL plasma using the QIAamp MinElute Virus spin kit (Qiagen, Frederick, MD). .. Extracted RNA was used for amplification using the SensiFAST Probe Lo-ROX One-Step Kit (Bioline BIO-78005, Taunton, MA) on a 7,500 Real-Time PCR system (Applied Biosystems, Foster City, CA).

    Article Title: Subclinical Infection of Macaques and Baboons with A Baboon Simarterivirus
    Article Snippet: .. For each animal, viral RNA was isolated from approximately 200 µL of plasma using the Qiagen QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany), omitting carrier RNA. cDNA synthesis and PCR were performed using random hexamers (for the inoculum) or with seven primer sets (for the remaining animals, see ) to amplify the entire SWBV genome in overlapping amplicons with the Superscript III One-Step RT-PCR kit with Platinum Taq Polymerase (Invitrogen, Carlsbad, CA, USA). .. All amplicons from a single sample were pooled to achieve 1 ng of total amplified DNA in 5 µL.

    Generated:

    Article Title: Subclinical Infection of Macaques and Baboons with A Baboon Simarterivirus
    Article Snippet: For each animal, viral RNA was isolated from approximately 200 µL of plasma using the Qiagen QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany), omitting carrier RNA. cDNA synthesis and PCR were performed using random hexamers (for the inoculum) or with seven primer sets (for the remaining animals, see ) to amplify the entire SWBV genome in overlapping amplicons with the Superscript III One-Step RT-PCR kit with Platinum Taq Polymerase (Invitrogen, Carlsbad, CA, USA). .. Pooled amplicons (or total cDNA generated from random-hexamer amplification) were fragmented, and sequencing adaptors were added using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA).

    Inhibition:

    Article Title: Invader Plus Method Detects Herpes Simplex Virus in Cerebrospinal Fluid and Simultaneously Differentiates Types 1 and 2
    Article Snippet: To ensure extraction recovery and inhibition detection, an internal control (IC) standard supplied with the HSV reagents was spiked into the samples before the start of the extraction procedure by adding 5 μl of IC to the lysis buffer. .. Nucleic acid from 200 μl of each CSF specimen was extracted by using a QIAamp MinElute virus spin kit (QIAGEN Inc.), according to the manufacturer's instructions ( ).

    Polymerase Chain Reaction:

    Article Title: Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
    Article Snippet: Paragraph title: PCR for viral DNA. ... Supernatant from fibroblasts infected with Toledo isolate passage 7 (P7) was used to extract viral DNA using the QIAamp MinElute virus spin kit (Qiagen).

    Article Title: P2Y6 Receptor-Mediated Spinal Microglial Activation in Neuropathic Pain
    Article Snippet: Total RNA was reverse transcribed into complementary DNA (cDNA) according to the instruction of QIAamp® MinElute® Virus Spin kit (Qiagen Inc USA). .. Then, the cDNA PCR reaction system was transferred into the 7300 thermocycler (Applied Biosystems) with the following program: 95°C for 5 min, 95°C for 10 s, 55°C for 10 s, and 75°C for 20 s, and a total of 40 cycles.

    Article Title: Molecular Epidemiologic Trends of Diarrhea‐Causing Virus Infection From Clinical Specimens in Cheonan, Korea, in 2010–2012
    Article Snippet: PCR was performed using the Seeplex diarrhea virus detection kit (Seegene Co., Seoul, Korea), according to the manufacturer's instructions, with a PTC‐200 PCR system (MJ Research, Hercules, CA). .. Distilled water (1 mL) was added to the stool specimen, and RNA was extracted from a 200 μL sample using a QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany). cDNA was synthesized from the extracted RNA using a RevertAid First Strand cDNA synthesis kit (Fermentas, Ontario, Canada). cDNA was synthesized in a 20 μL reaction containing 1 μL of random hexamer, 8 μL of total RNA, 1 μL of reverse transcriptase, 1 μL of RNase inhibitor, 2 μL of dNTP, 4 μL of 5× reverse transcription buffer, and 3 μL of distilled water at 37°C for 90 min.

    Article Title: Re-emergent Human Adenovirus Genome Type 7d Caused an Acute Respiratory Disease Outbreak in Southern China After a Twenty-one Year Absence
    Article Snippet: Detection of respiratory pathogens Total nucleic acids were extracted from the specimens using the QIAamp minElute virus spin kit (Qiagen; Hombrechtikon, Germany). .. For HAdV identification, type-specific primers were used to characterize the type by PCR, as described in an earlier report .

    Article Title: Subclinical Infection of Macaques and Baboons with A Baboon Simarterivirus
    Article Snippet: .. For each animal, viral RNA was isolated from approximately 200 µL of plasma using the Qiagen QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany), omitting carrier RNA. cDNA synthesis and PCR were performed using random hexamers (for the inoculum) or with seven primer sets (for the remaining animals, see ) to amplify the entire SWBV genome in overlapping amplicons with the Superscript III One-Step RT-PCR kit with Platinum Taq Polymerase (Invitrogen, Carlsbad, CA, USA). .. All amplicons from a single sample were pooled to achieve 1 ng of total amplified DNA in 5 µL.

    Binding Assay:

    Article Title: Safety and efficacy of anti-PD-L1 therapy in the woodchuck model of HBV infection
    Article Snippet: .. Circulating viral loads were determined by qPCR as previously described [ ], or alternatively by extraction of viral DNA using the QIAamp MinElute Virus Spin Kit (Qiagen) followed by qPCR with primers WHV-SL-F: 5’-CCTCGCAGGAGAAGATCTCAA-3’ , WHV-SL-R: 5’- GCAGTTGGCAGATGGAGATTG-3’ , and minor groove binding probe WHV-SL-MGB: 5’-6FAM-ACCGCGTCGCAGAC-3’ , within the core/polymerase overlapping region of the WHV genome. ..

    DNA Extraction:

    Article Title: Phage and Nucleocytoplasmic Large Viral Sequences Dominate Coral Viromes from the Arabian Gulf
    Article Snippet: Paragraph title: Viral DNA Extraction, Amplification and Sequencing ... The viral DNA was then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, United States) by following the manufacturer’s protocol.

    Isolation:

    Article Title: Identification and Genome Characterization of the First Sicinivirus Isolate from Chickens in Mainland China by Using Viral Metagenomics
    Article Snippet: Paragraph title: Virus nucleic acid isolation, Metagenomic library construction, sequencing and genome walking ... Viral nucleic acids were then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, Germany) according to the manufacturer’s instructions.

    Article Title: Human cytomegalovirus microRNAs are carried by virions and dense bodies and are delivered to target cells
    Article Snippet: .. DNA and RNA isolation from virions, DBs and cells Dialysed virions, DBs and mock samples were treated with micrococcal nuclease (2000 Kunitz units ml−1 ; Thermo Scientific) in the presence of 5 mM calcium chloride at 37 °C for 2 h. DNA was isolated with the QIAamp MinElute Virus Spin kit (Qiagen) without carrier RNA. .. RNA was isolated with the miRNeasy mini kit (Qiagen), including the DNase-I treatment or isolated with Trizol-LS reagent (Life Technologies).

    Article Title: Mauritian Cynomolgus Macaques Share Two Exceptionally Common Major Histocompatibility Complex Class I Alleles That Restrict Simian Immunodeficiency Virus-Specific CD8+ T Cells ▿ T Cells ▿ †
    Article Snippet: .. Viral RNA in the plasma was isolated by using the QIAamp MinElute virus spin kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. .. Viral RNA was reverse transcribed and amplified by using the One-Step reverse transcription-PCR kit (Qiagen), generating amplicons spanning the epitopes of interest from the SIVmac239 open reading frame.

    Article Title: A Recombinant Subunit Based Zika Virus Vaccine Is Efficacious in Non-human Primates
    Article Snippet: .. For the RT PCR (conducted on NHP samples), RNA was isolated from 200 μL plasma using the QIAamp MinElute Virus spin kit (Qiagen, Frederick, MD). .. Extracted RNA was used for amplification using the SensiFAST Probe Lo-ROX One-Step Kit (Bioline BIO-78005, Taunton, MA) on a 7,500 Real-Time PCR system (Applied Biosystems, Foster City, CA).

    Article Title: Subclinical Infection of Macaques and Baboons with A Baboon Simarterivirus
    Article Snippet: .. For each animal, viral RNA was isolated from approximately 200 µL of plasma using the Qiagen QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany), omitting carrier RNA. cDNA synthesis and PCR were performed using random hexamers (for the inoculum) or with seven primer sets (for the remaining animals, see ) to amplify the entire SWBV genome in overlapping amplicons with the Superscript III One-Step RT-PCR kit with Platinum Taq Polymerase (Invitrogen, Carlsbad, CA, USA). .. All amplicons from a single sample were pooled to achieve 1 ng of total amplified DNA in 5 µL.

    Multiplex PCR:

    Article Title: Molecular Epidemiologic Trends of Diarrhea‐Causing Virus Infection From Clinical Specimens in Cheonan, Korea, in 2010–2012
    Article Snippet: An mPCR assay was designed to detect the diarrhea‐causing viral pathogens as follows: open reading frame 1a (ORF1a) of HAstV that encodes the nonstructural polyprotein 1a as a viral protease and an RNA‐dependent RNA polymerase, the vp4 gene of GAR that encodes the protease‐sensitive glycoprotein VP4 of GAR genotype P, the hexon gene of EAdV that encodes the hexon coat protein found in adenoviruses, and ORF2 of NoV‐GI/GII that encodes the major capsid protein of NoV called VP1. .. Distilled water (1 mL) was added to the stool specimen, and RNA was extracted from a 200 μL sample using a QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany). cDNA was synthesized from the extracted RNA using a RevertAid First Strand cDNA synthesis kit (Fermentas, Ontario, Canada). cDNA was synthesized in a 20 μL reaction containing 1 μL of random hexamer, 8 μL of total RNA, 1 μL of reverse transcriptase, 1 μL of RNase inhibitor, 2 μL of dNTP, 4 μL of 5× reverse transcription buffer, and 3 μL of distilled water at 37°C for 90 min.

    Purification:

    Article Title: Complete Genome Sequence of a Rodent Torque Teno Virus in Hainan Island, China
    Article Snippet: Viral DNA was extracted from the liver tissue with the QIAamp MinElute virus spin kit (Qiagen), and sequence-independent amplification of viral nucleic acids was performed as described previously ( ). .. The amplicons in the 250 to 500-bp range were purified with a gel extraction kit (Tiangen).

    Sequencing:

    Article Title: Identification and Genome Characterization of the First Sicinivirus Isolate from Chickens in Mainland China by Using Viral Metagenomics
    Article Snippet: Paragraph title: Virus nucleic acid isolation, Metagenomic library construction, sequencing and genome walking ... Viral nucleic acids were then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, Germany) according to the manufacturer’s instructions.

    Article Title: Phage and Nucleocytoplasmic Large Viral Sequences Dominate Coral Viromes from the Arabian Gulf
    Article Snippet: Paragraph title: Viral DNA Extraction, Amplification and Sequencing ... The viral DNA was then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, United States) by following the manufacturer’s protocol.

    Article Title: Complete Genome Sequence of a Rodent Torque Teno Virus in Hainan Island, China
    Article Snippet: .. Viral DNA was extracted from the liver tissue with the QIAamp MinElute virus spin kit (Qiagen), and sequence-independent amplification of viral nucleic acids was performed as described previously ( ). .. The amplicons in the 250 to 500-bp range were purified with a gel extraction kit (Tiangen).

    Article Title: Mauritian Cynomolgus Macaques Share Two Exceptionally Common Major Histocompatibility Complex Class I Alleles That Restrict Simian Immunodeficiency Virus-Specific CD8+ T Cells ▿ T Cells ▿ †
    Article Snippet: Paragraph title: Viral sequencing. ... Viral RNA in the plasma was isolated by using the QIAamp MinElute virus spin kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.

    Article Title: Subclinical Infection of Macaques and Baboons with A Baboon Simarterivirus
    Article Snippet: Paragraph title: 2.5. Deep Sequencing ... For each animal, viral RNA was isolated from approximately 200 µL of plasma using the Qiagen QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany), omitting carrier RNA. cDNA synthesis and PCR were performed using random hexamers (for the inoculum) or with seven primer sets (for the remaining animals, see ) to amplify the entire SWBV genome in overlapping amplicons with the Superscript III One-Step RT-PCR kit with Platinum Taq Polymerase (Invitrogen, Carlsbad, CA, USA).

    Lysis:

    Article Title: Clinical, Virologic, and Immunologic Characteristics of Zika Virus Infection in a Cohort of US Patients: Prolonged RNA Detection in Whole Blood
    Article Snippet: ZIKV RNA Detection Serum and urine supernatant aliquots of 200 µL were stored at –70°C, whereas whole-blood packed cells, saliva, vaginal secretions, semen, and breast milk were mixed with lysis buffer with carrier RNA (Qiagen, Valencia, CA) before storing at –70°C. .. RNA was extracted using the QIAamp MinElute Virus Spin kit (Qiagen) following the original manufacturer protocol (serum) or with optimizations.

    Article Title: Invader Plus Method Detects Herpes Simplex Virus in Cerebrospinal Fluid and Simultaneously Differentiates Types 1 and 2
    Article Snippet: To ensure extraction recovery and inhibition detection, an internal control (IC) standard supplied with the HSV reagents was spiked into the samples before the start of the extraction procedure by adding 5 μl of IC to the lysis buffer. .. Nucleic acid from 200 μl of each CSF specimen was extracted by using a QIAamp MinElute virus spin kit (QIAGEN Inc.), according to the manufacturer's instructions ( ).

    Article Title: Optimizing PCR Detection of Zika Virus from Various Body Fluids
    Article Snippet: AVE buffer, the elution buffer from the QIAamp MinElute Virus Spin Kit (QIAGEN, Valencia, CA), was used as the spiking reference control buffer to generate expected cycle threshold (Ct) values. .. The aliquots were mixed with 200 µL of the lysis buffer from the kit, which is AL buffer containing 28 ng/µL of carrier RNA.

    Software:

    Article Title: P2Y6 Receptor-Mediated Spinal Microglial Activation in Neuropathic Pain
    Article Snippet: The primer sequences of target genes and internal reference gene β -actin were designed using Primer Premier 5.0 software and then synthesized by Sangon Biotech (Shanghai). .. Total RNA was reverse transcribed into complementary DNA (cDNA) according to the instruction of QIAamp® MinElute® Virus Spin kit (Qiagen Inc USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Safety and efficacy of anti-PD-L1 therapy in the woodchuck model of HBV infection
    Article Snippet: .. Circulating viral loads were determined by qPCR as previously described [ ], or alternatively by extraction of viral DNA using the QIAamp MinElute Virus Spin Kit (Qiagen) followed by qPCR with primers WHV-SL-F: 5’-CCTCGCAGGAGAAGATCTCAA-3’ , WHV-SL-R: 5’- GCAGTTGGCAGATGGAGATTG-3’ , and minor groove binding probe WHV-SL-MGB: 5’-6FAM-ACCGCGTCGCAGAC-3’ , within the core/polymerase overlapping region of the WHV genome. ..

    Article Title: Re-emergent Human Adenovirus Genome Type 7d Caused an Acute Respiratory Disease Outbreak in Southern China After a Twenty-one Year Absence
    Article Snippet: Detection of respiratory pathogens Total nucleic acids were extracted from the specimens using the QIAamp minElute virus spin kit (Qiagen; Hombrechtikon, Germany). .. Human adenovirus, respiratory syncytial virus, influenza virus A and B, parainfluenza virus types 1–3, human rhinovirus, human metapneumovirus, and human coronavirus OC43 and 229E were detected by real-time PCR as described earlier .

    Article Title: Invader Plus Method Detects Herpes Simplex Virus in Cerebrospinal Fluid and Simultaneously Differentiates Types 1 and 2
    Article Snippet: For analytical sensitivity experiments, serial dilutions of HSV-1 and HSV-2 viral DNA (Advanced Biotechnologies Inc.) quantified by real-time PCR ( , ) were made in a solution containing 20 ng/μl tRNA (Sigma-Aldrich, St. Louis, MO). .. Nucleic acid from 200 μl of each CSF specimen was extracted by using a QIAamp MinElute virus spin kit (QIAGEN Inc.), according to the manufacturer's instructions ( ).

    Article Title: A Recombinant Subunit Based Zika Virus Vaccine Is Efficacious in Non-human Primates
    Article Snippet: For the RT PCR (conducted on NHP samples), RNA was isolated from 200 μL plasma using the QIAamp MinElute Virus spin kit (Qiagen, Frederick, MD). .. Extracted RNA was used for amplification using the SensiFAST Probe Lo-ROX One-Step Kit (Bioline BIO-78005, Taunton, MA) on a 7,500 Real-Time PCR system (Applied Biosystems, Foster City, CA).

    Multiplex Assay:

    Article Title: Molecular Epidemiologic Trends of Diarrhea‐Causing Virus Infection From Clinical Specimens in Cheonan, Korea, in 2010–2012
    Article Snippet: Paragraph title: Multiplex RT‐PCR Analysis ... Distilled water (1 mL) was added to the stool specimen, and RNA was extracted from a 200 μL sample using a QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany). cDNA was synthesized from the extracted RNA using a RevertAid First Strand cDNA synthesis kit (Fermentas, Ontario, Canada). cDNA was synthesized in a 20 μL reaction containing 1 μL of random hexamer, 8 μL of total RNA, 1 μL of reverse transcriptase, 1 μL of RNase inhibitor, 2 μL of dNTP, 4 μL of 5× reverse transcription buffer, and 3 μL of distilled water at 37°C for 90 min.

    Agarose Gel Electrophoresis:

    Article Title: Molecular Epidemiologic Trends of Diarrhea‐Causing Virus Infection From Clinical Specimens in Cheonan, Korea, in 2010–2012
    Article Snippet: Distilled water (1 mL) was added to the stool specimen, and RNA was extracted from a 200 μL sample using a QIAamp MinElute virus spin kit (Qiagen, Hilden, Germany). cDNA was synthesized from the extracted RNA using a RevertAid First Strand cDNA synthesis kit (Fermentas, Ontario, Canada). cDNA was synthesized in a 20 μL reaction containing 1 μL of random hexamer, 8 μL of total RNA, 1 μL of reverse transcriptase, 1 μL of RNase inhibitor, 2 μL of dNTP, 4 μL of 5× reverse transcription buffer, and 3 μL of distilled water at 37°C for 90 min. .. The mPCR products were electrophoresed in a 2% agarose gel with ethidium bromide for 30 min at 100–150 V. The agarose gel was rinsed with distilled water, amplification was assessed by visualizing the gel on a UV transilluminator, and the results were analyzed after a photograph was taken (Fig. ).

    Incubation:

    Article Title: Identification and Genome Characterization of the First Sicinivirus Isolate from Chickens in Mainland China by Using Viral Metagenomics
    Article Snippet: Viral nucleic acids were then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, Germany) according to the manufacturer’s instructions. .. Briefly, 11 μl of extracted RNA was mixed with 1 μl of 10 mM dNTP Mixture and 1 μl of 100 μM primer KN or RA01N , incubated at 65°C for 5 min, and chilled on ice.

    Article Title: Clinical, Virologic, and Immunologic Characteristics of Zika Virus Infection in a Cohort of US Patients: Prolonged RNA Detection in Whole Blood
    Article Snippet: RNA was extracted using the QIAamp MinElute Virus Spin kit (Qiagen) following the original manufacturer protocol (serum) or with optimizations. .. Modifications included pretreatment of urine supernatant with Urine Conditioning Buffer (Zymo Research, Irvine, CA), and adjusted incubation with elution buffer (5 minutes at 56°C) for the remaining specimen types.

    Article Title: Phage and Nucleocytoplasmic Large Viral Sequences Dominate Coral Viromes from the Arabian Gulf
    Article Snippet: Viral DNA Extraction, Amplification and Sequencing To remove naked DNA from each sample, 2.5 U of DNase I (Sigma–Aldrich, United States) was added per 100 μl of sample, and the mixture was incubated for 1 h at 37°C ( ). .. The viral DNA was then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, United States) by following the manufacturer’s protocol.

    Concentration Assay:

    Article Title: Phage and Nucleocytoplasmic Large Viral Sequences Dominate Coral Viromes from the Arabian Gulf
    Article Snippet: The viral DNA was then extracted using the QIAamp MinElute Virus Spin Kit (Qiagen, United States) by following the manufacturer’s protocol. .. The DNA concentration was determined, and DNA purity was confirmed using a Nanodrop nucleic acid analyser (Thermo Scientific, United States).

    High Throughput Screening Assay:

    Article Title: Complete Genome Sequence of a Rodent Torque Teno Virus in Hainan Island, China
    Article Snippet: Viral DNA was extracted from the liver tissue with the QIAamp MinElute virus spin kit (Qiagen), and sequence-independent amplification of viral nucleic acids was performed as described previously ( ). .. Five hundred nanograms of DNA were fragmented with Covaris S2 shearing and subjected to high-throughput paired-end 2 × 100-bp sequencing on an Illumina HiSeq 2000 instrument.

    Gel Extraction:

    Article Title: Complete Genome Sequence of a Rodent Torque Teno Virus in Hainan Island, China
    Article Snippet: Viral DNA was extracted from the liver tissue with the QIAamp MinElute virus spin kit (Qiagen), and sequence-independent amplification of viral nucleic acids was performed as described previously ( ). .. The amplicons in the 250 to 500-bp range were purified with a gel extraction kit (Tiangen).

    Variant Assay:

    Article Title: Complete Genome Sequence of a Rodent Torque Teno Virus in Hainan Island, China
    Article Snippet: In this study, a genetic variant of RoTTV3, RoTTV3-HMU1 (Hainan Medical University 1), was identified in a rat ( Rattus norvegicus ) captured in a residential area of Haikou City, Hainan Province, southern China. .. Viral DNA was extracted from the liver tissue with the QIAamp MinElute virus spin kit (Qiagen), and sequence-independent amplification of viral nucleic acids was performed as described previously ( ).

    RNA Detection:

    Article Title: Clinical, Virologic, and Immunologic Characteristics of Zika Virus Infection in a Cohort of US Patients: Prolonged RNA Detection in Whole Blood
    Article Snippet: Paragraph title: ZIKV RNA Detection ... RNA was extracted using the QIAamp MinElute Virus Spin kit (Qiagen) following the original manufacturer protocol (serum) or with optimizations.

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    Qiagen qiaamp minelute virus spin kit
    Qiaamp Minelute Virus Spin Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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