qiaamp dna ffpe tissue kit 56404  (Qiagen)


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    Name:
    QIAamp DNA FFPE Tissue Kit
    Description:
    For purification of genomic DNA from formalin fixed paraffin embedded tissues Kit contents Qiagen QIAamp DNA FFPE Tissue Kit 50 preps 20 to 100L Elution Volume Up to 8 Sections Sample Formalin Fixed Paraffin Embedded Tissue Sample Silica Technology Genomic DNA Mitochondrial DNA Purification Spin Column Format Ideal for Real time PCR STR Analysis LMD PCR For Purification of Genomic DNA From Formalin fixed Paraffin embedded Tissues Includes 50 QIAamp MinElute Columns Proteinase K Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA Consistent high yields Complete removal of contaminants and inhibitor
    Catalog Number:
    56404
    Price:
    242
    Category:
    QIAamp DNA FFPE Tissue Kit
    Buy from Supplier


    Structured Review

    Qiagen qiaamp dna ffpe tissue kit 56404
    QIAamp DNA FFPE Tissue Kit
    For purification of genomic DNA from formalin fixed paraffin embedded tissues Kit contents Qiagen QIAamp DNA FFPE Tissue Kit 50 preps 20 to 100L Elution Volume Up to 8 Sections Sample Formalin Fixed Paraffin Embedded Tissue Sample Silica Technology Genomic DNA Mitochondrial DNA Purification Spin Column Format Ideal for Real time PCR STR Analysis LMD PCR For Purification of Genomic DNA From Formalin fixed Paraffin embedded Tissues Includes 50 QIAamp MinElute Columns Proteinase K Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA Consistent high yields Complete removal of contaminants and inhibitor
    https://www.bioz.com/result/qiaamp dna ffpe tissue kit 56404/product/Qiagen
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna ffpe tissue kit 56404 - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Clinicopathological features and prognostic roles of KRAS, BRAF, PIK3CA and NRAS mutations in advanced gastric cancer
    Article Snippet: DNA from pieces of the scratched tissue sample was isolated using the QIAamp DNA FFPE Tissue Kit (QIAGEN KK, Tokyo, Japan). .. Exon 2 (codon 12, 13), exon 3 (codon 61), exon 4 (codon 146) of KRAS gene and exon 15 (codon 600) of BRAF gene and exon 9 (codon 542, 545), exon 20 (codon 1047) of PIK3CA gene and exon 2 (codon 12, 13), exon 3 (codon 61) of NRAS gene were amplified by PCR (the GeneAmp PCR System 9700 thermal cycler).

    Article Title: Mutations in the Kinase Domain of the HER2/ERBB2 Gene Identified in a Wide Variety of Human Cancers
    Article Snippet: DNA was isolated from FFPE tissue using the QIAamp DNA FFPE tissue kit (Qiagen, Valencia, CA). .. Library preparation was performed using the Illumina TruSeq Amplicon Cancer panel and sequenced using the Illumina MiSeq platform.

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: OncoSreen ST2.0 is a 50-gene solid tumor panel that uses the Ion Ampliseq Cancer Hotspot Panel V2 primer set (Thermo Fisher Scientific) for amplification of 207 hot-spot targeted amplicons across 50 genes [ ]. .. DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen).

    Article Title: Detecting EGFR Mutations in Patients with Non-small Cell Lung Cancer
    Article Snippet: DNA was extracted from FFPE NSCLC samples from primary tumors by QIAamp DNA FFPE Tissue Kit (Qiagen Ltd.) for genomic DNA purification. .. The amplification reaction was done on the Rotor-Gene Q instrument (Qiagen Ltd.).

    Polymerase Chain Reaction:

    Article Title: Clinicopathological features and prognostic roles of KRAS, BRAF, PIK3CA and NRAS mutations in advanced gastric cancer
    Article Snippet: DNA from pieces of the scratched tissue sample was isolated using the QIAamp DNA FFPE Tissue Kit (QIAGEN KK, Tokyo, Japan). .. Exon 2 (codon 12, 13), exon 3 (codon 61), exon 4 (codon 146) of KRAS gene and exon 15 (codon 600) of BRAF gene and exon 9 (codon 542, 545), exon 20 (codon 1047) of PIK3CA gene and exon 2 (codon 12, 13), exon 3 (codon 61) of NRAS gene were amplified by PCR (the GeneAmp PCR System 9700 thermal cycler).

    Article Title: Clinical and immunophenotypic findings in 4 forms of equine lymphoma
    Article Snippet: DNA was extracted from paraffin-embedded intestinal biopsy samples according to the manufacturer’s instructions (QIAamp DNA FFPE Tissue Kit; Qiagen, PrecisionMed. .. A semi-nested polymerase chain reaction (PCR) protocol targeting a conserved fragment of glycoprotein B (gB) of Equid Herpesvirus type 5 (EHV-5) was performed , and the results from the intestine were positive.

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: To determine the presence of HPV-16 genomic sequences, PCR was conducted on DNA extracted from each sample. .. Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol.

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen). .. FFPE DNA was amplified for somatic mutations located within mutational hotspot regions of 50 cancer-related genes using multiplex PCR reagents (Thermo Fisher Scientific).

    Article Title: Heterozygous deletion of chromosome 17p renders prostate cancer vulnerable to inhibition of RNA polymerase II
    Article Snippet: Genomic DNA isolation and copy number validation Total genomic DNA was extracted either from cell lines using DNeasy Blood & Tissue Kit (Qiagen) or from human tissue specimen using QIAamp DNA FFPE Tissue Kit (Qiagen) according to the manufacturer’s instructions. .. The copy number variations for PSMB6, TP53, POLR2A , and FLCN were determined using TaqMan probes and TaqMan PCR kit.

    Article Title: Detecting EGFR Mutations in Patients with Non-small Cell Lung Cancer
    Article Snippet: DNA was extracted from FFPE NSCLC samples from primary tumors by QIAamp DNA FFPE Tissue Kit (Qiagen Ltd.) for genomic DNA purification. .. The therascreen EGFR RGQ PCR Kit (Qiagen Ltd.) was custom-made for detecting 29 somatic mutations in exons 18-21 of the EGFR gene using a RT-PCR technology.

    Article Title: Human Papillomavirus Infection, p16INK4a Expression and Genetic Alterations in Vietnamese Cervical Neuroendocrine Cancer
    Article Snippet: DNA Isolation DNA was isolated using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA). .. The quality of the DNA samples was assessed via polymerase chain reaction (PCR) targeting the β-globin gene.

    Article Title: Screening for major driver oncogene alterations in adenosquamous lung carcinoma using PCR coupled with next-generation and Sanger sequencing methods
    Article Snippet: DNA was isolated from paraffin-embedded tissues using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. Mutation profiles for EGFR, K-Ras, PIK3CA, and B-Raf were examined using the Human EGFR Gene Mutation Detection Kit (Real-time Fluorescent PCR), Human K-Ras Gene Mutation Detection Kit, Human PIK3CA Gene Mutation Detection Kit, and Human B-Raf Gene Mutation Detection Kit (Beijing ACCB Biotech, Beijing, China), respectively.

    Blocking Assay:

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol. .. The number of 4 μm paraffin slices used per sample depended on the size of the specimen sample in the block to help insure adequate DNA extraction.

    Real-time Polymerase Chain Reaction:

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen). .. Following extraction, DNA was quantified using the Qubit fluorometric assay (Thermo Fisher Scientific) and further assessed for quantity and quality using a quantitative PCR assay (hgDNA Quantitation and QC kit, KAPA Biosystems).

    Article Title: Primary tumor resection for initially staged IV breast cancer
    Article Snippet: DNA isolation from formalin fixed paraffin embedded (FFPE) samples was done by QIAamp DNA FFPE Tissue Kit (Qiagen, Venlo, Holland) according to the manufacturer's protocol. .. Quantitative real-time PCR experiments were performed using the Light Cycler 480 (Roche, Basel, Switzerland).

    Quantitation Assay:

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen). .. Following extraction, DNA was quantified using the Qubit fluorometric assay (Thermo Fisher Scientific) and further assessed for quantity and quality using a quantitative PCR assay (hgDNA Quantitation and QC kit, KAPA Biosystems).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Clinicopathological features and prognostic roles of KRAS, BRAF, PIK3CA and NRAS mutations in advanced gastric cancer
    Article Snippet: .. DNA from pieces of the scratched tissue sample was isolated using the QIAamp DNA FFPE Tissue Kit (QIAGEN KK, Tokyo, Japan). .. Exon 2 (codon 12, 13), exon 3 (codon 61), exon 4 (codon 146) of KRAS gene and exon 15 (codon 600) of BRAF gene and exon 9 (codon 542, 545), exon 20 (codon 1047) of PIK3CA gene and exon 2 (codon 12, 13), exon 3 (codon 61) of NRAS gene were amplified by PCR (the GeneAmp PCR System 9700 thermal cycler).

    Article Title: Mutations in the Kinase Domain of the HER2/ERBB2 Gene Identified in a Wide Variety of Human Cancers
    Article Snippet: .. DNA was isolated from FFPE tissue using the QIAamp DNA FFPE tissue kit (Qiagen, Valencia, CA). ..

    Article Title: Clinical and immunophenotypic findings in 4 forms of equine lymphoma
    Article Snippet: .. DNA was extracted from paraffin-embedded intestinal biopsy samples according to the manufacturer’s instructions (QIAamp DNA FFPE Tissue Kit; Qiagen, PrecisionMed. ..

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: .. Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol. .. The number of 4 μm paraffin slices used per sample depended on the size of the specimen sample in the block to help insure adequate DNA extraction.

    Article Title: DAXX mutations as potential genomic markers of malignant evolution in small nonfunctioning pancreatic neuroendocrine tumors
    Article Snippet: .. Thus, DNA was extracted from FFPE sections of 10 μm in thickness using the QIAamp DNA FFPE Tissue Kit (Qiagen, Germantown, MD) and quantified by Qubit DNA HS Assay (Thermo Fisher Scientific, Waltham, MA). .. The purity of the DNA was measured by NanoDrop ND-2000 (Thermo Fisher Scientific).

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: .. DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen). .. Following extraction, DNA was quantified using the Qubit fluorometric assay (Thermo Fisher Scientific) and further assessed for quantity and quality using a quantitative PCR assay (hgDNA Quantitation and QC kit, KAPA Biosystems).

    Article Title: Fetal microchimerism in human brain tumors
    Article Snippet: .. Genomic DNA was isolated from formalin-fixed paraffin-embedded tissue sections using the QIAamp DNA FFPE tissue kit (Qiagen, Hilden, Germany). .. DNA was isolated following the manufacturer instructions with minor modifications.

    Article Title: Clinical characterization of ERBB2 exon 20 insertions and heterogeneity of outcomes responding to afatinib in Chinese lung cancer patients
    Article Snippet: .. Preparation of tissue DNA and plasma cfDNA Tissue DNA was extracted using the QIAamp DNA FFPE tissue kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. Circulating cfDNA was recovered from 4 to 5 mL of plasma by using the QIAamp Circulating Nucleic Acid kit (Qiagen).

    Article Title: Heterozygous deletion of chromosome 17p renders prostate cancer vulnerable to inhibition of RNA polymerase II
    Article Snippet: .. Genomic DNA isolation and copy number validation Total genomic DNA was extracted either from cell lines using DNeasy Blood & Tissue Kit (Qiagen) or from human tissue specimen using QIAamp DNA FFPE Tissue Kit (Qiagen) according to the manufacturer’s instructions. .. The copy number variations for PSMB6, TP53, POLR2A , and FLCN were determined using TaqMan probes and TaqMan PCR kit.

    Article Title: Primary tumor resection for initially staged IV breast cancer
    Article Snippet: .. DNA isolation from formalin fixed paraffin embedded (FFPE) samples was done by QIAamp DNA FFPE Tissue Kit (Qiagen, Venlo, Holland) according to the manufacturer's protocol. .. Bisulfite conversion of FFPE DNA from each sample (500 ng) was performed using an EZ DNA Methylation-Gold kit according to the manufacturer's protocol (Zymo Research, Irvine, CA).

    Article Title: Detecting EGFR Mutations in Patients with Non-small Cell Lung Cancer
    Article Snippet: .. DNA was extracted from FFPE NSCLC samples from primary tumors by QIAamp DNA FFPE Tissue Kit (Qiagen Ltd.) for genomic DNA purification. .. The concentrations of the DNA samples were measured by NanoDrop Lite spectrophotometer system (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Human Papillomavirus Infection, p16INK4a Expression and Genetic Alterations in Vietnamese Cervical Neuroendocrine Cancer
    Article Snippet: .. DNA Isolation DNA was isolated using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA). .. The quality of the DNA samples was assessed via polymerase chain reaction (PCR) targeting the β-globin gene.

    Article Title: Screening for major driver oncogene alterations in adenosquamous lung carcinoma using PCR coupled with next-generation and Sanger sequencing methods
    Article Snippet: .. DNA was isolated from paraffin-embedded tissues using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. ..

    Article Title: Primary glioblastoma cells for precision medicine: a quantitative portrait of genomic (in)stability during the first 30 passages
    Article Snippet: .. DNA was isolated from the GCs and formalin-fixed paraffin-embedded (FFPE) GBM tissues by using the DNeasy Blood & Tissue Kit (Qiagen) and the QIAamp DNA FFPE Tissue Kit (Qiagen) according to manufacturer’s protocol. .. Total RNA was extracted from GCs by the miRNeasy Mini Kit (Qiagen).

    Activity Assay:

    Article Title: Clinical and immunophenotypic findings in 4 forms of equine lymphoma
    Article Snippet: Histologic examination of mesenteric lymph nodes indicated chronic necrotic-hemorrhagic lymphadenitis with evidence of infiltration by a population of homogeneous lymphoid cells associated with increased mitotic activity; rare plasma cells were also observed. .. DNA was extracted from paraffin-embedded intestinal biopsy samples according to the manufacturer’s instructions (QIAamp DNA FFPE Tissue Kit; Qiagen, PrecisionMed.

    Activated Clotting Time Assay:

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol. .. PCR was then performed using primers for HPV-16 E6 (Forward: ATG CAC CAA AAG AGA ACT G Reverse: TTA CAG CTG GGT TTC TCT AC) and E7 (Forward: ATG CAT GGA GAT ACA CCT AC Reverse: TTATGG TTT CTG AGA ACA GAT GG) annealing at 47 °C.

    Derivative Assay:

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: Samples that met criteria for inclusion were then re-cut and underwent immunohistochemistry (IHC) for p16 (mouse anti-human, SC-56330, Santa Cruz Biotechnology, Dallas, TX) using a protocol derived from the Yale University School of Medicine and the Immunocruz mouse ABC staining system (SC-2017, Santa Cruz Biotechnology, Dallas, TX). .. Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol.

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: The OncoScreen ST2.0 clinical assay was performed on tissue derived from the wide local excision scalp melanoma. .. DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen).

    Immunohistochemistry:

    Article Title: Clinical and immunophenotypic findings in 4 forms of equine lymphoma
    Article Snippet: Immunohistochemistry (IHC) of the intestine and lymph node tissue with antibodies against CD3 (Dako, 1:200; Agilent, Santa Clara, California, USA) and CD20 (Thermo Scientific, 1:200; Thermo Fisher Scientific, Rodano Milano, Italy) antigens was performed ( ). .. DNA was extracted from paraffin-embedded intestinal biopsy samples according to the manufacturer’s instructions (QIAamp DNA FFPE Tissue Kit; Qiagen, PrecisionMed.

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: To control for variability in staining, each IHC run included an identical positive cervical cancer control. .. Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol.

    Genomic Sequencing:

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: To determine the presence of HPV-16 genomic sequences, PCR was conducted on DNA extracted from each sample. .. Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol.

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: Paragraph title: Next-generation genomic sequencing ... DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen).

    DNA HS Assay:

    Article Title: DAXX mutations as potential genomic markers of malignant evolution in small nonfunctioning pancreatic neuroendocrine tumors
    Article Snippet: .. Thus, DNA was extracted from FFPE sections of 10 μm in thickness using the QIAamp DNA FFPE Tissue Kit (Qiagen, Germantown, MD) and quantified by Qubit DNA HS Assay (Thermo Fisher Scientific, Waltham, MA). .. The purity of the DNA was measured by NanoDrop ND-2000 (Thermo Fisher Scientific).

    Sequencing:

    Article Title: Mutations in the Kinase Domain of the HER2/ERBB2 Gene Identified in a Wide Variety of Human Cancers
    Article Snippet: Paragraph title: Next-Generation Sequence Analysis ... DNA was isolated from FFPE tissue using the QIAamp DNA FFPE tissue kit (Qiagen, Valencia, CA).

    Cellular Antioxidant Activity Assay:

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol. .. PCR was then performed using primers for HPV-16 E6 (Forward: ATG CAC CAA AAG AGA ACT G Reverse: TTA CAG CTG GGT TTC TCT AC) and E7 (Forward: ATG CAT GGA GAT ACA CCT AC Reverse: TTATGG TTT CTG AGA ACA GAT GG) annealing at 47 °C.

    DNA Extraction:

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol. .. The number of 4 μm paraffin slices used per sample depended on the size of the specimen sample in the block to help insure adequate DNA extraction.

    Article Title: DAXX mutations as potential genomic markers of malignant evolution in small nonfunctioning pancreatic neuroendocrine tumors
    Article Snippet: Paragraph title: Pathological analysis and DNA extraction ... Thus, DNA was extracted from FFPE sections of 10 μm in thickness using the QIAamp DNA FFPE Tissue Kit (Qiagen, Germantown, MD) and quantified by Qubit DNA HS Assay (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Heterozygous deletion of chromosome 17p renders prostate cancer vulnerable to inhibition of RNA polymerase II
    Article Snippet: .. Genomic DNA isolation and copy number validation Total genomic DNA was extracted either from cell lines using DNeasy Blood & Tissue Kit (Qiagen) or from human tissue specimen using QIAamp DNA FFPE Tissue Kit (Qiagen) according to the manufacturer’s instructions. .. The copy number variations for PSMB6, TP53, POLR2A , and FLCN were determined using TaqMan probes and TaqMan PCR kit.

    Article Title: Primary tumor resection for initially staged IV breast cancer
    Article Snippet: .. DNA isolation from formalin fixed paraffin embedded (FFPE) samples was done by QIAamp DNA FFPE Tissue Kit (Qiagen, Venlo, Holland) according to the manufacturer's protocol. .. Bisulfite conversion of FFPE DNA from each sample (500 ng) was performed using an EZ DNA Methylation-Gold kit according to the manufacturer's protocol (Zymo Research, Irvine, CA).

    Article Title: Human Papillomavirus Infection, p16INK4a Expression and Genetic Alterations in Vietnamese Cervical Neuroendocrine Cancer
    Article Snippet: .. DNA Isolation DNA was isolated using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA). .. The quality of the DNA samples was assessed via polymerase chain reaction (PCR) targeting the β-globin gene.

    Methylation:

    Article Title: Primary tumor resection for initially staged IV breast cancer
    Article Snippet: Paragraph title: PD-L1 methylation analysis ... DNA isolation from formalin fixed paraffin embedded (FFPE) samples was done by QIAamp DNA FFPE Tissue Kit (Qiagen, Venlo, Holland) according to the manufacturer's protocol.

    Mutagenesis:

    Article Title: Screening for major driver oncogene alterations in adenosquamous lung carcinoma using PCR coupled with next-generation and Sanger sequencing methods
    Article Snippet: DNA was isolated from paraffin-embedded tissues using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. Mutation profiles for EGFR, K-Ras, PIK3CA, and B-Raf were examined using the Human EGFR Gene Mutation Detection Kit (Real-time Fluorescent PCR), Human K-Ras Gene Mutation Detection Kit, Human PIK3CA Gene Mutation Detection Kit, and Human B-Raf Gene Mutation Detection Kit (Beijing ACCB Biotech, Beijing, China), respectively.

    Isolation:

    Article Title: Clinicopathological features and prognostic roles of KRAS, BRAF, PIK3CA and NRAS mutations in advanced gastric cancer
    Article Snippet: .. DNA from pieces of the scratched tissue sample was isolated using the QIAamp DNA FFPE Tissue Kit (QIAGEN KK, Tokyo, Japan). .. Exon 2 (codon 12, 13), exon 3 (codon 61), exon 4 (codon 146) of KRAS gene and exon 15 (codon 600) of BRAF gene and exon 9 (codon 542, 545), exon 20 (codon 1047) of PIK3CA gene and exon 2 (codon 12, 13), exon 3 (codon 61) of NRAS gene were amplified by PCR (the GeneAmp PCR System 9700 thermal cycler).

    Article Title: Mutations in the Kinase Domain of the HER2/ERBB2 Gene Identified in a Wide Variety of Human Cancers
    Article Snippet: .. DNA was isolated from FFPE tissue using the QIAamp DNA FFPE tissue kit (Qiagen, Valencia, CA). ..

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: .. DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen). .. Following extraction, DNA was quantified using the Qubit fluorometric assay (Thermo Fisher Scientific) and further assessed for quantity and quality using a quantitative PCR assay (hgDNA Quantitation and QC kit, KAPA Biosystems).

    Article Title: Fetal microchimerism in human brain tumors
    Article Snippet: .. Genomic DNA was isolated from formalin-fixed paraffin-embedded tissue sections using the QIAamp DNA FFPE tissue kit (Qiagen, Hilden, Germany). .. DNA was isolated following the manufacturer instructions with minor modifications.

    Article Title: Human Papillomavirus Infection, p16INK4a Expression and Genetic Alterations in Vietnamese Cervical Neuroendocrine Cancer
    Article Snippet: .. DNA Isolation DNA was isolated using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA). .. The quality of the DNA samples was assessed via polymerase chain reaction (PCR) targeting the β-globin gene.

    Article Title: Screening for major driver oncogene alterations in adenosquamous lung carcinoma using PCR coupled with next-generation and Sanger sequencing methods
    Article Snippet: .. DNA was isolated from paraffin-embedded tissues using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. ..

    Article Title: Primary glioblastoma cells for precision medicine: a quantitative portrait of genomic (in)stability during the first 30 passages
    Article Snippet: .. DNA was isolated from the GCs and formalin-fixed paraffin-embedded (FFPE) GBM tissues by using the DNeasy Blood & Tissue Kit (Qiagen) and the QIAamp DNA FFPE Tissue Kit (Qiagen) according to manufacturer’s protocol. .. Total RNA was extracted from GCs by the miRNeasy Mini Kit (Qiagen).

    Microscopy:

    Article Title: Clinicopathological features and prognostic roles of KRAS, BRAF, PIK3CA and NRAS mutations in advanced gastric cancer
    Article Snippet: Tumor cell-rich areas in the hematoxylin and eosin section were marked under a microscope, and tissue was scratched from the area of another deparaffinized unstained section. .. DNA from pieces of the scratched tissue sample was isolated using the QIAamp DNA FFPE Tissue Kit (QIAGEN KK, Tokyo, Japan).

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: Pictures of all stained samples were taken with a photographic microscope in Maputo (Leica) and were reviewed electronically by an experienced head and neck pathologist at Yale (MP). .. Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol.

    Purification:

    Article Title: Droplet digital PCR measurement of HER2 in patients with gastric cancer
    Article Snippet: .. DNA was extracted and purified with the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. .. Extraction of serum ctDNA Blood samples were collected in tubes (vacutainer #367819, BD, Franklin Lakes, NJ, USA) and processed within 1 h after collection.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Detecting EGFR Mutations in Patients with Non-small Cell Lung Cancer
    Article Snippet: DNA was extracted from FFPE NSCLC samples from primary tumors by QIAamp DNA FFPE Tissue Kit (Qiagen Ltd.) for genomic DNA purification. .. The therascreen EGFR RGQ PCR Kit (Qiagen Ltd.) was custom-made for detecting 29 somatic mutations in exons 18-21 of the EGFR gene using a RT-PCR technology.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol. .. PCR was then performed using primers for HPV-16 E6 (Forward: ATG CAC CAA AAG AGA ACT G Reverse: TTA CAG CTG GGT TTC TCT AC) and E7 (Forward: ATG CAT GGA GAT ACA CCT AC Reverse: TTATGG TTT CTG AGA ACA GAT GG) annealing at 47 °C.

    Multiplex Assay:

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen). .. FFPE DNA was amplified for somatic mutations located within mutational hotspot regions of 50 cancer-related genes using multiplex PCR reagents (Thermo Fisher Scientific).

    Agarose Gel Electrophoresis:

    Article Title: Clinicopathological features and prognostic roles of KRAS, BRAF, PIK3CA and NRAS mutations in advanced gastric cancer
    Article Snippet: DNA from pieces of the scratched tissue sample was isolated using the QIAamp DNA FFPE Tissue Kit (QIAGEN KK, Tokyo, Japan). .. The PCR products were visualized using agarose gel electrophoresis with ethidium bromide staining and directly sequenced using an ABI 3130x/ Genetic Analyzer (Life Technologies Japan (Applied Biosystems), Tokyo, Japan) according to the manufacturer’s instructions.

    Next-Generation Sequencing:

    Article Title: Secondary resistance to immunotherapy associated with β-catenin pathway activation or PTEN loss in metastatic melanoma
    Article Snippet: Tissue slides and blocks were reviewed by a pathologist to select the appropriate material for NGS testing. .. DNA was isolated from micro-dissected FFPE tumor tissue using the QIAamp DNA FFPE Tissue Kit (Qiagen).

    Laser Capture Microdissection:

    Article Title: Screening for major driver oncogene alterations in adenosquamous lung carcinoma using PCR coupled with next-generation and Sanger sequencing methods
    Article Snippet: Genetic alteration detection The two components of ASC, which could be separated in HE slides, were dissected by the same pathologist using laser capture microdissection (PixCell II; Arcturus Engineering, Mountain View, CA) or manual macrodissection. .. DNA was isolated from paraffin-embedded tissues using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Spectrophotometry:

    Article Title: Detecting EGFR Mutations in Patients with Non-small Cell Lung Cancer
    Article Snippet: DNA was extracted from FFPE NSCLC samples from primary tumors by QIAamp DNA FFPE Tissue Kit (Qiagen Ltd.) for genomic DNA purification. .. The concentrations of the DNA samples were measured by NanoDrop Lite spectrophotometer system (Thermo Fisher Scientific, Waltham, MA, USA).

    DNA Methylation Assay:

    Article Title: Primary tumor resection for initially staged IV breast cancer
    Article Snippet: DNA isolation from formalin fixed paraffin embedded (FFPE) samples was done by QIAamp DNA FFPE Tissue Kit (Qiagen, Venlo, Holland) according to the manufacturer's protocol. .. The DNA methylation of PD-L1 was determined using a qMSP assay.

    DNA Purification:

    Article Title: Detecting EGFR Mutations in Patients with Non-small Cell Lung Cancer
    Article Snippet: .. DNA was extracted from FFPE NSCLC samples from primary tumors by QIAamp DNA FFPE Tissue Kit (Qiagen Ltd.) for genomic DNA purification. .. The concentrations of the DNA samples were measured by NanoDrop Lite spectrophotometer system (Thermo Fisher Scientific, Waltham, MA, USA).

    CTG Assay:

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol. .. PCR was then performed using primers for HPV-16 E6 (Forward: ATG CAC CAA AAG AGA ACT G Reverse: TTA CAG CTG GGT TTC TCT AC) and E7 (Forward: ATG CAT GGA GAT ACA CCT AC Reverse: TTATGG TTT CTG AGA ACA GAT GG) annealing at 47 °C.

    Staining:

    Article Title: Clinicopathological features and prognostic roles of KRAS, BRAF, PIK3CA and NRAS mutations in advanced gastric cancer
    Article Snippet: DNA from pieces of the scratched tissue sample was isolated using the QIAamp DNA FFPE Tissue Kit (QIAGEN KK, Tokyo, Japan). .. The PCR products were visualized using agarose gel electrophoresis with ethidium bromide staining and directly sequenced using an ABI 3130x/ Genetic Analyzer (Life Technologies Japan (Applied Biosystems), Tokyo, Japan) according to the manufacturer’s instructions.

    Article Title: Investigation of the presence of HPV related oropharyngeal and oral tongue squamous cell carcinoma in Mozambique
    Article Snippet: Pictures of all stained samples were taken with a photographic microscope in Maputo (Leica) and were reviewed electronically by an experienced head and neck pathologist at Yale (MP). .. Briefly, DNA was extracted from the formalin fixed paraffin embedded samples utilizing the QIAamp DNA FFPE Tissue Kit and Deparaffinization Solution (Qiagen, Venlo, the Netherlands) according to manufacturer protocol.

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  • 90
    Qiagen qiaamp dna ffpe tissue kit
    Qiaamp Dna Ffpe Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 707 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 707 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna ffpe tissue kit - by Bioz Stars, 2020-01
    90/100 stars
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