truchip chromatin shearing reagent kit  (Covaris)


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    Name:
    truChIP Chromatin Shearing Tissue Kit
    Description:
    The truChIP Chromatin Shearing Tissue Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from tissues 20 120mg using the Covaris Adaptive Focused Acoustics AFA technology
    Catalog Number:
    520237
    Price:
    None
    Applications:
    Chromatin shearing from tissue
    Category:
    Reagent
    Quantity:
    1 0
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    Structured Review

    Covaris truchip chromatin shearing reagent kit
    truChIP Chromatin Shearing Tissue Kit
    The truChIP Chromatin Shearing Tissue Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from tissues 20 120mg using the Covaris Adaptive Focused Acoustics AFA technology
    https://www.bioz.com/result/truchip chromatin shearing reagent kit/product/Covaris
    Average 95 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    truchip chromatin shearing reagent kit - by Bioz Stars, 2021-02
    95/100 stars

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    Related Articles

    Isolation:

    Article Title: Purβ promotes hepatic glucose production by increasing Adcy6 transcription
    Article Snippet: .. Their nuclei were isolated and subjected to sonication (M220 Focused-ultrasonicator; Covaris) to break genomic DNA into 500- to 1000-bp fragments using a chromatin shearing kit (520127 truChIP Chromatin Shearing Kit, Covaris). .. The samples were immunoprecipitated with Flag beads (A2220, Sigma).

    Article Title: A Novel t(8;14)(q24;q11) Rearranged Human Cell Line as a Model for Mechanistic and Drug Discovery Studies of NOTCH1-Independent Human T-Cell Leukemia
    Article Snippet: .. Nuclei were isolated and chromatin was purified by chemical lysis (truChIP Chromatin Shearing Reagent KIT, Covaris, Woburn, MA, USA) and processed as previously described [ ]. .. Statistical analysis: We performed statistical analysis by Student’s t -test and Mann-Whitney U test where appropriate.

    Purification:

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: .. The purified cells were cross-linked for 10 min with 1% formaldehyde in medium, were processed with a truChIP Chromatin Shearing Reagent Kit (Covaris) and sonicated for 5 min on Covaris S2 ultrasonicator. .. The sheared chromatin from 3–5 × 106 CD8+ cells was immunoprecipitated with 5 μg of purified anti-CBF-β antibody or control rabbit IgG and was washed as described .

    Article Title: A Novel t(8;14)(q24;q11) Rearranged Human Cell Line as a Model for Mechanistic and Drug Discovery Studies of NOTCH1-Independent Human T-Cell Leukemia
    Article Snippet: .. Nuclei were isolated and chromatin was purified by chemical lysis (truChIP Chromatin Shearing Reagent KIT, Covaris, Woburn, MA, USA) and processed as previously described [ ]. .. Statistical analysis: We performed statistical analysis by Student’s t -test and Mann-Whitney U test where appropriate.

    Chromatin Immunoprecipitation:

    Article Title: Concurrent Control of the Kaposi's Sarcoma-Associated Herpesvirus Life Cycle through Chromatin Modulation and Host Hedgehog Signaling: a New Prospect for the Therapeutic Potential of Lipoxin A4
    Article Snippet: .. To detect the direct association of SMARCB1 with viral DNA, a chromatin immunoprecipitation (ChIP) assay was performed using a truChIP chromatin shearing kit (Covaris, Woburn, MA). .. Briefly, untreated BCBL-1 and BC-3 cells (3 × 107 ) or cells treated with LXA4 for 48 h were fixed with 1% (vol/vol) methanol-free formaldehyde in fixing buffer for 5 min, cross-linking was quenched using quenching buffer for 5 min at room temperature, and the cells were washed twice with cold PBS and then incubated in lysis buffer to break the cell membrane.

    Article Title: SP1 and RARα regulate AGAP2 expression in cancer
    Article Snippet: .. Then, chromatin immunoprecipitation (ChIP) was performed using the truChIP™ Chromatin Shearing Reagent kit from Covaris and MAGnify™ Chromatin Immunoprecipitation System from Invitrogen and following the manufacturer’s instructions. .. The chromatin was sheared to 100–600 bp by sonication using an AFA Focused-Ultrasonicator (S220- Series from CovarisTM ) at 6 × 60 second on-off pulses at 4 °C.

    Article Title: Selective targeting of the BRG/PB1 bromodomains impairs embryonic and trophoblast stem cell maintenance
    Article Snippet: .. Chromatin immunoprecipitation ChIP experiments were carried out with Covaris truChIP chromatin shearing reagent according to the manufacturer’s instructions with certain modifications. ..

    Sonication:

    Article Title: Purβ promotes hepatic glucose production by increasing Adcy6 transcription
    Article Snippet: .. Their nuclei were isolated and subjected to sonication (M220 Focused-ultrasonicator; Covaris) to break genomic DNA into 500- to 1000-bp fragments using a chromatin shearing kit (520127 truChIP Chromatin Shearing Kit, Covaris). .. The samples were immunoprecipitated with Flag beads (A2220, Sigma).

    Article Title: Runx3 guards cytotoxic CD8+ effector T cells against deviation towards TFH cell lineage
    Article Snippet: .. The purified cells were cross-linked for 10 min with 1% formaldehyde in medium, were processed with a truChIP Chromatin Shearing Reagent Kit (Covaris) and sonicated for 5 min on Covaris S2 ultrasonicator. .. The sheared chromatin from 3–5 × 106 CD8+ cells was immunoprecipitated with 5 μg of purified anti-CBF-β antibody or control rabbit IgG and was washed as described .

    Lysis:

    Article Title: A Novel t(8;14)(q24;q11) Rearranged Human Cell Line as a Model for Mechanistic and Drug Discovery Studies of NOTCH1-Independent Human T-Cell Leukemia
    Article Snippet: .. Nuclei were isolated and chromatin was purified by chemical lysis (truChIP Chromatin Shearing Reagent KIT, Covaris, Woburn, MA, USA) and processed as previously described [ ]. .. Statistical analysis: We performed statistical analysis by Student’s t -test and Mann-Whitney U test where appropriate.

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  • Bioz Stars
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  • 95
    Covaris truchip chromatin shearing reagent kit
    Truchip Chromatin Shearing Reagent Kit, supplied by Covaris, used in various techniques. Bioz Stars score: 95/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truchip chromatin shearing reagent kit/product/Covaris
    Average 95 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    truchip chromatin shearing reagent kit - by Bioz Stars, 2021-02
    95/100 stars
      Buy from Supplier

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