millitubes  (Covaris)


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    Structured Review

    Covaris millitubes
    Millitubes, supplied by Covaris, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/millitubes/product/Covaris
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    millitubes - by Bioz Stars, 2019-09
    92/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Binding of estrogen receptors to switch sites and regulatory elements in the immunoglobulin heavy chain locus of activated B cells suggests a direct influence of estrogen on antibody expression
    Article Snippet: The pellet was resuspended in Covaris shearing buffer plus PIs at a concentration of 1 ml per initial 2 X 107 cells and sheared in the Covaris E210 (Covaris) in Covaris MilliTubes under the following conditions, 200 cycles/burst, 20 W for 30 minutes. .. The pellet was resuspended in Covaris shearing buffer plus PIs at a concentration of 1 ml per initial 2 X 107 cells and sheared in the Covaris E210 (Covaris) in Covaris MilliTubes under the following conditions, 200 cycles/burst, 20 W for 30 minutes.

    Isolation:

    Article Title: BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses
    Article Snippet: Cells were washed and incubated in nucleus isolation buffer (10 mM HEPES, pH 7.6, 85 mM KCl, 0.5% NP‐40). .. Nuclear pellets (~2 × 107 ) were re‐suspended in 1 ml shearing buffer (1× TE, pH 8, and 0.1% SDS) and transferred to Covaris millitubes and sonicated in Covaris S200 sonicator.

    Article Title: PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance
    Article Snippet: Chromatin was prepared from isolated acinar cells or cell lines using the truChIP chromatin-shearing reagent kit (Covaris, 520154). .. Chromatin was sheared in 1-mL millitubes (Covaris, 520135) using a Covaris S200 (5% duty cycle, intensity of 4, 200 cycles per burst, for 8 min) to obtain chromatin fragments 100–700 base pairs in length.

    Spectrophotometry:

    Article Title: In Vitro Cytokine Licensing Induces Persistent Permissive Chromatin at the IDO1 Promoter
    Article Snippet: 1.0 mL aliquots were transferred to milliTUBEs (520132, Covaris) and sonicated for 16 minutes at 4–10°C according to manufacturer’s instructions using a Covaris M220 focused ultrasonicator (500295, High Cell Protocol, Covaris) to achieve an average chromatin fragment length of ≤ 700 bp. .. 1.0 mL aliquots were transferred to milliTUBEs (520132, Covaris) and sonicated for 16 minutes at 4–10°C according to manufacturer’s instructions using a Covaris M220 focused ultrasonicator (500295, High Cell Protocol, Covaris) to achieve an average chromatin fragment length of ≤ 700 bp.

    Purification:

    Article Title: BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses
    Article Snippet: Nuclear pellets (~2 × 107 ) were re‐suspended in 1 ml shearing buffer (1× TE, pH 8, and 0.1% SDS) and transferred to Covaris millitubes and sonicated in Covaris S200 sonicator. .. Nuclear pellets (~2 × 107 ) were re‐suspended in 1 ml shearing buffer (1× TE, pH 8, and 0.1% SDS) and transferred to Covaris millitubes and sonicated in Covaris S200 sonicator.

    Concentration Assay:

    Article Title: In Vitro Cytokine Licensing Induces Persistent Permissive Chromatin at the IDO1 Promoter
    Article Snippet: 1.0 mL aliquots were transferred to milliTUBEs (520132, Covaris) and sonicated for 16 minutes at 4–10°C according to manufacturer’s instructions using a Covaris M220 focused ultrasonicator (500295, High Cell Protocol, Covaris) to achieve an average chromatin fragment length of ≤ 700 bp. .. DNA was quantitated following phenol:chloroform:isoamyl alcohol (PCI), pH 8.0, extraction using a nanodrop spectrophotometer (Thermo Scientific, Rockford, IL).

    Article Title: Binding of estrogen receptors to switch sites and regulatory elements in the immunoglobulin heavy chain locus of activated B cells suggests a direct influence of estrogen on antibody expression
    Article Snippet: Nuclei were centrifuged at 1500× g for 5 minutes and subjected to a series of washes in Covaris wash buffer and shearing buffer with PIs. .. The pellet was resuspended in Covaris shearing buffer plus PIs at a concentration of 1 ml per initial 2 X 107 cells and sheared in the Covaris E210 (Covaris) in Covaris MilliTubes under the following conditions, 200 cycles/burst, 20 W for 30 minutes. .. Sheared chromatin was diluted 1:3 with Covaris ChIP dilution buffer and immunoprecipitated with anti-estrogen receptor α antibody (Abcam #32063), 5 μg/2 X 107 cell equivalents, overnight with rotation at 4°C.

    Incubation:

    Article Title: A comprehensive analysis of 195 DNA methylomes reveals shared and cell-specific features of partially methylated domains
    Article Snippet: Nuclei from cell pellets containing about four million of cells have been extracted by sonication [ ] using the following parameters: 75 W peak power, 2% duty factor, 200 cycles/burst, and 180 s, using Covaris milliTubes and Covaris E220 sonicator. .. Nuclei from cell pellets containing about four million of cells have been extracted by sonication [ ] using the following parameters: 75 W peak power, 2% duty factor, 200 cycles/burst, and 180 s, using Covaris milliTubes and Covaris E220 sonicator.

    Article Title: BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses
    Article Snippet: Cells were washed and incubated in nucleus isolation buffer (10 mM HEPES, pH 7.6, 85 mM KCl, 0.5% NP‐40). .. Nuclear pellets (~2 × 107 ) were re‐suspended in 1 ml shearing buffer (1× TE, pH 8, and 0.1% SDS) and transferred to Covaris millitubes and sonicated in Covaris S200 sonicator.

    Article Title: Binding of estrogen receptors to switch sites and regulatory elements in the immunoglobulin heavy chain locus of activated B cells suggests a direct influence of estrogen on antibody expression
    Article Snippet: The pellet was resuspended in Covaris shearing buffer plus PIs at a concentration of 1 ml per initial 2 X 107 cells and sheared in the Covaris E210 (Covaris) in Covaris MilliTubes under the following conditions, 200 cycles/burst, 20 W for 30 minutes. .. Sheared chromatin was diluted 1:3 with Covaris ChIP dilution buffer and immunoprecipitated with anti-estrogen receptor α antibody (Abcam #32063), 5 μg/2 X 107 cell equivalents, overnight with rotation at 4°C.

    Immunoprecipitation:

    Article Title: BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses
    Article Snippet: Paragraph title: Immunoprecipitation and ChIP‐seq ... Nuclear pellets (~2 × 107 ) were re‐suspended in 1 ml shearing buffer (1× TE, pH 8, and 0.1% SDS) and transferred to Covaris millitubes and sonicated in Covaris S200 sonicator.

    Article Title: PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance
    Article Snippet: Chromatin was sheared in 1-mL millitubes (Covaris, 520135) using a Covaris S200 (5% duty cycle, intensity of 4, 200 cycles per burst, for 8 min) to obtain chromatin fragments 100–700 base pairs in length. .. Complexes were isolated using 50 μL of Dynabeads (Invitrogen, 10004D) previously blocked with 1 mg/mL BSA.

    Cell Culture:

    Article Title: Binding of estrogen receptors to switch sites and regulatory elements in the immunoglobulin heavy chain locus of activated B cells suggests a direct influence of estrogen on antibody expression
    Article Snippet: Cultured B cells were harvested and treated with 2 mM disuccinimidyl glutarate (DSG, ProteoChem) in DPBS (Lonza) with the following proteinase inhibitors (PIs); PMSF (Sigma), Pepstatin A (Sigma), and Leupeptin (Sigma). .. The pellet was resuspended in Covaris shearing buffer plus PIs at a concentration of 1 ml per initial 2 X 107 cells and sheared in the Covaris E210 (Covaris) in Covaris MilliTubes under the following conditions, 200 cycles/burst, 20 W for 30 minutes.

    Sonication:

    Article Title: A comprehensive analysis of 195 DNA methylomes reveals shared and cell-specific features of partially methylated domains
    Article Snippet: Hi-C experiments have been conducted as previously described [ ], with the following modifications. .. Nuclei from cell pellets containing about four million of cells have been extracted by sonication [ ] using the following parameters: 75 W peak power, 2% duty factor, 200 cycles/burst, and 180 s, using Covaris milliTubes and Covaris E220 sonicator. .. After nuclei permeabilization, chromatin has been digested overnight at 37∘ C using HindIII high fidelity (80 units per million cells; R3104S, NEB).

    Article Title: BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses
    Article Snippet: Cells were washed and incubated in nucleus isolation buffer (10 mM HEPES, pH 7.6, 85 mM KCl, 0.5% NP‐40). .. Nuclear pellets (~2 × 107 ) were re‐suspended in 1 ml shearing buffer (1× TE, pH 8, and 0.1% SDS) and transferred to Covaris millitubes and sonicated in Covaris S200 sonicator. .. Sonicated chromatin was precipitated with antibodies pre‐bound to Dynabeads Protein G (Thermo Fisher).

    Article Title: In Vitro Cytokine Licensing Induces Persistent Permissive Chromatin at the IDO1 Promoter
    Article Snippet: Cross-linked nuclei were lysed for 10 minutes on a rotator at 4°C, and washed with cold PBS. .. 1.0 mL aliquots were transferred to milliTUBEs (520132, Covaris) and sonicated for 16 minutes at 4–10°C according to manufacturer’s instructions using a Covaris M220 focused ultrasonicator (500295, High Cell Protocol, Covaris) to achieve an average chromatin fragment length of ≤ 700 bp. .. Sheared chromatin was spun at 1,700 × g for 5 minutes at 4°C, transferred to microcentrifuge tubes on ice and snap-frozen.

    Polymerase Chain Reaction:

    Article Title: BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses
    Article Snippet: Nuclear pellets (~2 × 107 ) were re‐suspended in 1 ml shearing buffer (1× TE, pH 8, and 0.1% SDS) and transferred to Covaris millitubes and sonicated in Covaris S200 sonicator. .. Nuclear pellets (~2 × 107 ) were re‐suspended in 1 ml shearing buffer (1× TE, pH 8, and 0.1% SDS) and transferred to Covaris millitubes and sonicated in Covaris S200 sonicator.

    Article Title: PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance
    Article Snippet: Chromatin was sheared in 1-mL millitubes (Covaris, 520135) using a Covaris S200 (5% duty cycle, intensity of 4, 200 cycles per burst, for 8 min) to obtain chromatin fragments 100–700 base pairs in length. .. Two nanograms of immunoprecipitated DNA was prepared as a standard Illumina library using the ThruPLEX DNA-seq kit (Rubicon) according to the manufacturer's protocol.

    Lysis:

    Article Title: A comprehensive analysis of 195 DNA methylomes reveals shared and cell-specific features of partially methylated domains
    Article Snippet: Nuclei from cell pellets containing about four million of cells have been extracted by sonication [ ] using the following parameters: 75 W peak power, 2% duty factor, 200 cycles/burst, and 180 s, using Covaris milliTubes and Covaris E220 sonicator. .. Nuclei from cell pellets containing about four million of cells have been extracted by sonication [ ] using the following parameters: 75 W peak power, 2% duty factor, 200 cycles/burst, and 180 s, using Covaris milliTubes and Covaris E220 sonicator.

    Chromatin Immunoprecipitation:

    Article Title: In Vitro Cytokine Licensing Induces Persistent Permissive Chromatin at the IDO1 Promoter
    Article Snippet: Paragraph title: Chromatin IP ... 1.0 mL aliquots were transferred to milliTUBEs (520132, Covaris) and sonicated for 16 minutes at 4–10°C according to manufacturer’s instructions using a Covaris M220 focused ultrasonicator (500295, High Cell Protocol, Covaris) to achieve an average chromatin fragment length of ≤ 700 bp.

    Article Title: Binding of estrogen receptors to switch sites and regulatory elements in the immunoglobulin heavy chain locus of activated B cells suggests a direct influence of estrogen on antibody expression
    Article Snippet: Paragraph title: 2.3 Chromatin preparation for ChIP library ... The pellet was resuspended in Covaris shearing buffer plus PIs at a concentration of 1 ml per initial 2 X 107 cells and sheared in the Covaris E210 (Covaris) in Covaris MilliTubes under the following conditions, 200 cycles/burst, 20 W for 30 minutes.

    Hi-C:

    Article Title: A comprehensive analysis of 195 DNA methylomes reveals shared and cell-specific features of partially methylated domains
    Article Snippet: Paragraph title: HepG2 Hi-C ... Nuclei from cell pellets containing about four million of cells have been extracted by sonication [ ] using the following parameters: 75 W peak power, 2% duty factor, 200 cycles/burst, and 180 s, using Covaris milliTubes and Covaris E220 sonicator.

    ChIP-sequencing:

    Article Title: BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses
    Article Snippet: Paragraph title: Immunoprecipitation and ChIP‐seq ... Nuclear pellets (~2 × 107 ) were re‐suspended in 1 ml shearing buffer (1× TE, pH 8, and 0.1% SDS) and transferred to Covaris millitubes and sonicated in Covaris S200 sonicator.

    Article Title: PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance
    Article Snippet: Paragraph title: ChIP-seq ... Chromatin was sheared in 1-mL millitubes (Covaris, 520135) using a Covaris S200 (5% duty cycle, intensity of 4, 200 cycles per burst, for 8 min) to obtain chromatin fragments 100–700 base pairs in length.

    DNA Sequencing:

    Article Title: PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance
    Article Snippet: Chromatin was sheared in 1-mL millitubes (Covaris, 520135) using a Covaris S200 (5% duty cycle, intensity of 4, 200 cycles per burst, for 8 min) to obtain chromatin fragments 100–700 base pairs in length. .. Complexes were isolated using 50 μL of Dynabeads (Invitrogen, 10004D) previously blocked with 1 mg/mL BSA.

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  • 96
    Covaris microtube 15 afa beads strip v2
    Microtube 15 Afa Beads Strip V2, supplied by Covaris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtube 15 afa beads strip v2/product/Covaris
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microtube 15 afa beads strip v2 - by Bioz Stars, 2019-09
    96/100 stars
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    Covaris acoustic shearing instrument
    Overview of data generation and collection. <t>*Illumina</t> acquired extracted <t>DNA</t> from ATCC for library construction, sequencing, and analysis.
    Acoustic Shearing Instrument, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acoustic shearing instrument/product/Covaris
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    acoustic shearing instrument - by Bioz Stars, 2019-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Overview of data generation and collection. *Illumina acquired extracted DNA from ATCC for library construction, sequencing, and analysis.

    Journal: Scientific Reports

    Article Title: A somatic reference standard for cancer genome sequencing

    doi: 10.1038/srep24607

    Figure Lengend Snippet: Overview of data generation and collection. *Illumina acquired extracted DNA from ATCC for library construction, sequencing, and analysis.

    Article Snippet: 500 ng of each DNA was prepared using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina® , cat# FC-121-3001) following the manufacturer’s protocol with modified Covaris DNA shearing settings: Duty cycle 10%, Cycles/Burst 200, Time 45 sec.

    Techniques: Sequencing