unshared dna  (Covaris)


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    Name:
    g TUBE 10
    Description:
    g TUBE is a single use device that shears genomic DNA into selected fragments sizes ranging from 6kb to 20kb The only equipment needed is a compatible bench top centrifuge 10 individually wrapped g TUBES for shearing DNA 6kb to 20 kb and one g TUBE Prep Station
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    Structured Review

    Covaris unshared dna
    g TUBE 10
    g TUBE is a single use device that shears genomic DNA into selected fragments sizes ranging from 6kb to 20kb The only equipment needed is a compatible bench top centrifuge 10 individually wrapped g TUBES for shearing DNA 6kb to 20 kb and one g TUBE Prep Station
    https://www.bioz.com/result/unshared dna/product/Covaris
    Average 88 stars, based on 138 article reviews
    Price from $9.99 to $1999.99
    unshared dna - by Bioz Stars, 2020-08
    88/100 stars

    Images

    1) Product Images from "Improved data analysis for the MinION nanopore sequencer"

    Article Title: Improved data analysis for the MinION nanopore sequencer

    Journal: Nature methods

    doi: 10.1038/nmeth.3290

    Resolution of CT47 repeat copy number estimate on human chromosome Xq24. (a) BAC end sequence alignments (RP11-482A22: AQ630638 and AZ517599) span a 247 kb region, including thirteen annotated CT47 genes (each defined within a 4.8 kb tandem repeat) and a 50 kb scaffold gap in the GRCh38/hg38 reference assembly. (b) Utilizing MinION long-reads obtained from RP11-482A22 high-molecular weight BAC DNA, nine reads span the length of the CT47-repeat region providing evidence for eight tandem copies of the CT47-repeat. Insert size estimate (170–175 kb, as determined by pulse-field gel electrophoresis) is noted as a dotted line, with flanking regions (upstream: 57 kb and downstream region: 73 kb, black line) and repeat region (37-to-42 kb, or 7.5-to-8.75 copies of the repeat, blue line). Single copy regions directly before the CT47 repeats are shown in orange (6.6 kb) and green (2.6 kb), repeat copies are labeled in blue, and grey lines describe read alignment into flanking region. The size of the repeat region are provided on the left (range 36 kb ’ 42 kb). (c) Shearing the BAC DNA to increase sequence coverage provided copy number estimates by read depth. All bases not included in the CT47 repeat unit are labeled as flanking region (grey distribution, mean: 46.2 base coverage). Base coverage across the CT47 repeats are summarized over one copy of the repeat to provide an estimate of the combined number (dark blue distribution, mean: 329.3 base coverage), and are similar to single copy estimates when normalized for eight copies (light blue distribution, mean: 41.15 base coverage).
    Figure Legend Snippet: Resolution of CT47 repeat copy number estimate on human chromosome Xq24. (a) BAC end sequence alignments (RP11-482A22: AQ630638 and AZ517599) span a 247 kb region, including thirteen annotated CT47 genes (each defined within a 4.8 kb tandem repeat) and a 50 kb scaffold gap in the GRCh38/hg38 reference assembly. (b) Utilizing MinION long-reads obtained from RP11-482A22 high-molecular weight BAC DNA, nine reads span the length of the CT47-repeat region providing evidence for eight tandem copies of the CT47-repeat. Insert size estimate (170–175 kb, as determined by pulse-field gel electrophoresis) is noted as a dotted line, with flanking regions (upstream: 57 kb and downstream region: 73 kb, black line) and repeat region (37-to-42 kb, or 7.5-to-8.75 copies of the repeat, blue line). Single copy regions directly before the CT47 repeats are shown in orange (6.6 kb) and green (2.6 kb), repeat copies are labeled in blue, and grey lines describe read alignment into flanking region. The size of the repeat region are provided on the left (range 36 kb ’ 42 kb). (c) Shearing the BAC DNA to increase sequence coverage provided copy number estimates by read depth. All bases not included in the CT47 repeat unit are labeled as flanking region (grey distribution, mean: 46.2 base coverage). Base coverage across the CT47 repeats are summarized over one copy of the repeat to provide an estimate of the combined number (dark blue distribution, mean: 329.3 base coverage), and are similar to single copy estimates when normalized for eight copies (light blue distribution, mean: 41.15 base coverage).

    Techniques Used: BAC Assay, Sequencing, Molecular Weight, Nucleic Acid Electrophoresis, Labeling

    2) Product Images from "Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing"

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    Journal: Standards in Genomic Sciences

    doi: 10.1186/s40793-017-0239-1

    PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures
    Figure Legend Snippet: PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures

    Techniques Used: Magnetic Beads

    3) Product Images from "Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing"

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    Journal: Standards in Genomic Sciences

    doi: 10.1186/s40793-017-0239-1

    PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures
    Figure Legend Snippet: PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures

    Techniques Used: Magnetic Beads

    4) Product Images from "Improved data analysis for the MinION nanopore sequencer"

    Article Title: Improved data analysis for the MinION nanopore sequencer

    Journal: Nature methods

    doi: 10.1038/nmeth.3290

    Resolution of CT47 repeat copy number estimate on human chromosome Xq24. (a) BAC end sequence alignments (RP11-482A22: AQ630638 and AZ517599) span a 247 kb region, including thirteen annotated CT47 genes (each defined within a 4.8 kb tandem repeat) and a 50 kb scaffold gap in the GRCh38/hg38 reference assembly. (b) Utilizing MinION long-reads obtained from RP11-482A22 high-molecular weight BAC DNA, nine reads span the length of the CT47-repeat region providing evidence for eight tandem copies of the CT47-repeat. Insert size estimate (170–175 kb, as determined by pulse-field gel electrophoresis) is noted as a dotted line, with flanking regions (upstream: 57 kb and downstream region: 73 kb, black line) and repeat region (37-to-42 kb, or 7.5-to-8.75 copies of the repeat, blue line). Single copy regions directly before the CT47 repeats are shown in orange (6.6 kb) and green (2.6 kb), repeat copies are labeled in blue, and grey lines describe read alignment into flanking region. The size of the repeat region are provided on the left (range 36 kb ’ 42 kb). (c) Shearing the BAC DNA to increase sequence coverage provided copy number estimates by read depth. All bases not included in the CT47 repeat unit are labeled as flanking region (grey distribution, mean: 46.2 base coverage). Base coverage across the CT47 repeats are summarized over one copy of the repeat to provide an estimate of the combined number (dark blue distribution, mean: 329.3 base coverage), and are similar to single copy estimates when normalized for eight copies (light blue distribution, mean: 41.15 base coverage).
    Figure Legend Snippet: Resolution of CT47 repeat copy number estimate on human chromosome Xq24. (a) BAC end sequence alignments (RP11-482A22: AQ630638 and AZ517599) span a 247 kb region, including thirteen annotated CT47 genes (each defined within a 4.8 kb tandem repeat) and a 50 kb scaffold gap in the GRCh38/hg38 reference assembly. (b) Utilizing MinION long-reads obtained from RP11-482A22 high-molecular weight BAC DNA, nine reads span the length of the CT47-repeat region providing evidence for eight tandem copies of the CT47-repeat. Insert size estimate (170–175 kb, as determined by pulse-field gel electrophoresis) is noted as a dotted line, with flanking regions (upstream: 57 kb and downstream region: 73 kb, black line) and repeat region (37-to-42 kb, or 7.5-to-8.75 copies of the repeat, blue line). Single copy regions directly before the CT47 repeats are shown in orange (6.6 kb) and green (2.6 kb), repeat copies are labeled in blue, and grey lines describe read alignment into flanking region. The size of the repeat region are provided on the left (range 36 kb ’ 42 kb). (c) Shearing the BAC DNA to increase sequence coverage provided copy number estimates by read depth. All bases not included in the CT47 repeat unit are labeled as flanking region (grey distribution, mean: 46.2 base coverage). Base coverage across the CT47 repeats are summarized over one copy of the repeat to provide an estimate of the combined number (dark blue distribution, mean: 329.3 base coverage), and are similar to single copy estimates when normalized for eight copies (light blue distribution, mean: 41.15 base coverage).

    Techniques Used: BAC Assay, Sequencing, Molecular Weight, Nucleic Acid Electrophoresis, Labeling

    5) Product Images from "Assessing structural variation in a personal genome—towards a human reference diploid genome"

    Article Title: Assessing structural variation in a personal genome—towards a human reference diploid genome

    Journal: BMC Genomics

    doi: 10.1186/s12864-015-1479-3

    Size distribution. All HS1011 SV events larger than 100 bp and less than 100,000 bp were compared to events from the Venter genome (HuRef) and an Asian Male (YH), both specifically characterized for SV content. In this size regime, the HS1011, HuRef, and YH samples contain 5044, 5127, and 5374 deletions (panel a ) and 4482, 4479, and 15525 insertions (panel b ), respectively. The YH SV distributions are based on de novo assembly of 35 bp single-end and paired end data. This assembly was used to identify SVs between 1 bp and 50 kbp. Initial events larger than 50 bp were filtered using discordant paired-end mapping of ~35 bp reads. Given the relative abundance of HS1011 sequence data (including both long reads and longer short reads as compared to the YH short reads), and given the differences in methods, it is unlikely that the ~3-fold difference in insertions between the YH set and the HS1011 and HuRef sets represents a significant lack of Parliament sensitivity.
    Figure Legend Snippet: Size distribution. All HS1011 SV events larger than 100 bp and less than 100,000 bp were compared to events from the Venter genome (HuRef) and an Asian Male (YH), both specifically characterized for SV content. In this size regime, the HS1011, HuRef, and YH samples contain 5044, 5127, and 5374 deletions (panel a ) and 4482, 4479, and 15525 insertions (panel b ), respectively. The YH SV distributions are based on de novo assembly of 35 bp single-end and paired end data. This assembly was used to identify SVs between 1 bp and 50 kbp. Initial events larger than 50 bp were filtered using discordant paired-end mapping of ~35 bp reads. Given the relative abundance of HS1011 sequence data (including both long reads and longer short reads as compared to the YH short reads), and given the differences in methods, it is unlikely that the ~3-fold difference in insertions between the YH set and the HS1011 and HuRef sets represents a significant lack of Parliament sensitivity.

    Techniques Used: End-sequence Profiling, Sequencing

    6) Product Images from "Firefly genomes illuminate parallel origins of bioluminescence in beetles"

    Article Title: Firefly genomes illuminate parallel origins of bioluminescence in beetles

    Journal: eLife

    doi: 10.7554/eLife.36495

    PFGE of P. pyralis HMW DNA used for PacBio sequencing. Lane 1 was used for further library prep and sequencing, Lanes 2–5 represent separate batches of P. pyralis HMW DNA that was not used for PacBio sequencing. Lane 1 was used as it had the highest DNA yield, and an equivalent DNA size distribution to the other samples.
    Figure Legend Snippet: PFGE of P. pyralis HMW DNA used for PacBio sequencing. Lane 1 was used for further library prep and sequencing, Lanes 2–5 represent separate batches of P. pyralis HMW DNA that was not used for PacBio sequencing. Lane 1 was used as it had the highest DNA yield, and an equivalent DNA size distribution to the other samples.

    Techniques Used: Sequencing

    7) Product Images from "A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits"

    Article Title: A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkv773

    Quantitative assessment of the enrichment process with qPCR. ( A ) Absolute concentration (copies/ul) of targeted (dark blue) and untargeted (light blue) regions of Phage Lambda genome with respect to the combination of the baits (‘S’-short, ‘M’-medium, ‘L’-long) and the DNA shearing size (5 Kbp or 10 Kbp) used during the enrichment. The same regions were also amplified using control (pre-enriched) DNA as template. ( B ) Ratio of the concentration of the targeted regions versus the concentration of the untargeted regions.
    Figure Legend Snippet: Quantitative assessment of the enrichment process with qPCR. ( A ) Absolute concentration (copies/ul) of targeted (dark blue) and untargeted (light blue) regions of Phage Lambda genome with respect to the combination of the baits (‘S’-short, ‘M’-medium, ‘L’-long) and the DNA shearing size (5 Kbp or 10 Kbp) used during the enrichment. The same regions were also amplified using control (pre-enriched) DNA as template. ( B ) Ratio of the concentration of the targeted regions versus the concentration of the untargeted regions.

    Techniques Used: Real-time Polymerase Chain Reaction, Concentration Assay, Amplification

    ( A ) Raw (per-base) coverage in whole genome sequencing of Phage Lambda . ( B ) Raw (per-base) coverage after enriching the sequencing library for a specific region (41 053 – 42 351 bp, dark gray column). ( C ) Normalized coverage across the genome (segregated in overlapping-by-100 bp 200 bp-sized windows). Enriched and whole genome library coverage is plotted with dashed and solid line, respectively. The higher coverage across the last 5 kb of the genome (light gray column) corresponds to the internal control DNA added to both libraries.
    Figure Legend Snippet: ( A ) Raw (per-base) coverage in whole genome sequencing of Phage Lambda . ( B ) Raw (per-base) coverage after enriching the sequencing library for a specific region (41 053 – 42 351 bp, dark gray column). ( C ) Normalized coverage across the genome (segregated in overlapping-by-100 bp 200 bp-sized windows). Enriched and whole genome library coverage is plotted with dashed and solid line, respectively. The higher coverage across the last 5 kb of the genome (light gray column) corresponds to the internal control DNA added to both libraries.

    Techniques Used: Sequencing

    8) Product Images from "Firefly genomes illuminate parallel origins of bioluminescence in beetles"

    Article Title: Firefly genomes illuminate parallel origins of bioluminescence in beetles

    Journal: eLife

    doi: 10.7554/eLife.36495

    PFGE of P. pyralis HMW DNA used for PacBio sequencing. Lane 1 was used for further library prep and sequencing, Lanes 2–5 represent separate batches of P. pyralis HMW DNA that was not used for PacBio sequencing. Lane 1 was used as it had the highest DNA yield, and an equivalent DNA size distribution to the other samples.
    Figure Legend Snippet: PFGE of P. pyralis HMW DNA used for PacBio sequencing. Lane 1 was used for further library prep and sequencing, Lanes 2–5 represent separate batches of P. pyralis HMW DNA that was not used for PacBio sequencing. Lane 1 was used as it had the highest DNA yield, and an equivalent DNA size distribution to the other samples.

    Techniques Used: Sequencing

    9) Product Images from "High-throughput long paired-end sequencing of a Fosmid library by PacBio"

    Article Title: High-throughput long paired-end sequencing of a Fosmid library by PacBio

    Journal: Plant Methods

    doi: 10.1186/s13007-019-0525-6

    The pipeline of Fosmid-size long paired-end library construction. The red area represents the vector, the blue area represents the large inserted genomic fragment, and the yellow area represents the Ampicillin resistance gene tag. The Fosmid clones were pooled together, and DNA was extracted for paired-end library construction. Pooled Fosmid plasmid DNA was sheared into ~ 15 kb fragments by g-TUBE (Covaris). It generated insert only, vector with single-ends and vector with paired-ends. Then, these DNA fragments were end repaired and gel purified for ligation with the Ampicillin resistance gene tag. Although all fragments could be ligated to the Ampicillin resistance gene tag, only those containing the chloramphenicol resistant gene and ori V ligated to an Amp tag were screened out with double resistance to chloramphenicol and ampicillin after transformation. Finally, the vector was removed by I-SceI and the paired-end fragments with the Amp tag were sequenced on PacBio
    Figure Legend Snippet: The pipeline of Fosmid-size long paired-end library construction. The red area represents the vector, the blue area represents the large inserted genomic fragment, and the yellow area represents the Ampicillin resistance gene tag. The Fosmid clones were pooled together, and DNA was extracted for paired-end library construction. Pooled Fosmid plasmid DNA was sheared into ~ 15 kb fragments by g-TUBE (Covaris). It generated insert only, vector with single-ends and vector with paired-ends. Then, these DNA fragments were end repaired and gel purified for ligation with the Ampicillin resistance gene tag. Although all fragments could be ligated to the Ampicillin resistance gene tag, only those containing the chloramphenicol resistant gene and ori V ligated to an Amp tag were screened out with double resistance to chloramphenicol and ampicillin after transformation. Finally, the vector was removed by I-SceI and the paired-end fragments with the Amp tag were sequenced on PacBio

    Techniques Used: Plasmid Preparation, Clone Assay, Generated, Purification, Ligation, Transformation Assay

    Related Articles

    Marker:

    Article Title: Genomic and probiotic characterization of SJP-SNU strain of Pichia kudriavzevii
    Article Snippet: .. Briefly, 10 μg of yeast genomic DNA was sheared with a Covaris® g-TUBE® device and size-selection for 15–50 kb was performed with a BluePippin system (0.75% DF Marker S1 high-pass 15–20 kb), both done according to the manufacturer’s protocols. .. SMRTbell template libraries were subsequently prepared using the commercial Template Preparation Kit from Pacific Biosciences Inc. and involved the sequential steps of DNA end repair, adapter ligation and exonuclease digestion of incompletely ligated products.

    Centrifugation:

    Article Title: Efficient DNA Fingerprinting Based on the Targeted Sequencing of Active Retrotransposon Insertion Sites Using a Bench-Top High-Throughput Sequencing Platform
    Article Snippet: .. MiSeq library construction Genomic DNA (5 μg) was separated into fragments of ∼6 kb by g-TUBE (Covaris) centrifugation and purified using a QIAquick PCR Purification Kit (Qiagen). .. The purified products were treated with DNA polymerase I and T4 DNA polymerase to convert the heterogeneous ends via their physical fragmentation into blunt ends.

    Magnetic Beads:

    Article Title: Expanding an expanded genome: long-read sequencing of Trypanosoma cruzi
    Article Snippet: .. Briefly, DNA was mechanically fragmented using a Covaris g-TUBE device, and concentrated with AMPure PB magnetic beads. .. The final long-insert PacBio libraries were size-selected for fragments larger than 10 kb using the BluePippin device.

    Next-Generation Sequencing:

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
    Article Snippet: .. The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes. ..

    Purification:

    Article Title: Efficient DNA Fingerprinting Based on the Targeted Sequencing of Active Retrotransposon Insertion Sites Using a Bench-Top High-Throughput Sequencing Platform
    Article Snippet: .. MiSeq library construction Genomic DNA (5 μg) was separated into fragments of ∼6 kb by g-TUBE (Covaris) centrifugation and purified using a QIAquick PCR Purification Kit (Qiagen). .. The purified products were treated with DNA polymerase I and T4 DNA polymerase to convert the heterogeneous ends via their physical fragmentation into blunt ends.

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
    Article Snippet: .. The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes. ..

    Polymerase Chain Reaction:

    Article Title: Efficient DNA Fingerprinting Based on the Targeted Sequencing of Active Retrotransposon Insertion Sites Using a Bench-Top High-Throughput Sequencing Platform
    Article Snippet: .. MiSeq library construction Genomic DNA (5 μg) was separated into fragments of ∼6 kb by g-TUBE (Covaris) centrifugation and purified using a QIAquick PCR Purification Kit (Qiagen). .. The purified products were treated with DNA polymerase I and T4 DNA polymerase to convert the heterogeneous ends via their physical fragmentation into blunt ends.

    Sequencing:

    Article Title: Complete Genome Sequence of emm1 Streptococcus pyogenes 10-85, a Strain Isolated from a Patient with Streptococcal Toxic Shock Syndrome in Japan
    Article Snippet: .. The genomic DNA was fragmented prior to PacBio RS II sequencing using the Covaris g-TUBE device (Woburn, MA), in accordance with the manufacturer’s instructions. .. PacBio RS II sequencing runs were performed using the PacBio SMRTbell template prep kit 1.0 and polymerase binding kit P6 after size selection using BluePippin (Sage Science, Beverly, MA) with a cutoff value of 15 kb.

    Concentration Assay:

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
    Article Snippet: .. The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes. ..

    Software:

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
    Article Snippet: .. The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes. ..

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    Covaris g tube device qc
    PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using <t>Covaris</t> <t>g-TUBE</t> and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures
    G Tube Device Qc, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g tube device qc/product/Covaris
    Average 99 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    g tube device qc - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    91
    Covaris tissuetube tt05 25
    PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using <t>Covaris</t> <t>g-TUBE</t> and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures
    Tissuetube Tt05 25, supplied by Covaris, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tissuetube tt05 25/product/Covaris
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tissuetube tt05 25 - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures

    Article Snippet: The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes.

    Techniques: Magnetic Beads

    The pipeline of Fosmid-size long paired-end library construction. The red area represents the vector, the blue area represents the large inserted genomic fragment, and the yellow area represents the Ampicillin resistance gene tag. The Fosmid clones were pooled together, and DNA was extracted for paired-end library construction. Pooled Fosmid plasmid DNA was sheared into ~ 15 kb fragments by g-TUBE (Covaris). It generated insert only, vector with single-ends and vector with paired-ends. Then, these DNA fragments were end repaired and gel purified for ligation with the Ampicillin resistance gene tag. Although all fragments could be ligated to the Ampicillin resistance gene tag, only those containing the chloramphenicol resistant gene and ori V ligated to an Amp tag were screened out with double resistance to chloramphenicol and ampicillin after transformation. Finally, the vector was removed by I-SceI and the paired-end fragments with the Amp tag were sequenced on PacBio

    Journal: Plant Methods

    Article Title: High-throughput long paired-end sequencing of a Fosmid library by PacBio

    doi: 10.1186/s13007-019-0525-6

    Figure Lengend Snippet: The pipeline of Fosmid-size long paired-end library construction. The red area represents the vector, the blue area represents the large inserted genomic fragment, and the yellow area represents the Ampicillin resistance gene tag. The Fosmid clones were pooled together, and DNA was extracted for paired-end library construction. Pooled Fosmid plasmid DNA was sheared into ~ 15 kb fragments by g-TUBE (Covaris). It generated insert only, vector with single-ends and vector with paired-ends. Then, these DNA fragments were end repaired and gel purified for ligation with the Ampicillin resistance gene tag. Although all fragments could be ligated to the Ampicillin resistance gene tag, only those containing the chloramphenicol resistant gene and ori V ligated to an Amp tag were screened out with double resistance to chloramphenicol and ampicillin after transformation. Finally, the vector was removed by I-SceI and the paired-end fragments with the Amp tag were sequenced on PacBio

    Article Snippet: A total of 400 μg of pooled Fosmid DNA was sheared into fragments by g-TUBE (Covaris), with a mean size ranging from 6 to 20 kb.

    Techniques: Plasmid Preparation, Clone Assay, Generated, Purification, Ligation, Transformation Assay