qiaamp fast dna stool mini kit (Qiagen)

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Name:

QIAamp Fast DNA Stool Mini Kit

Description:

For isolation of gDNA from stool samples Kit contents For 50 DNA preps 50 QIAamp Mini Spin Columns QIAGEN Proteinase K InhibitEXBuffer wash and elution buffers Collection Tubes 2 ml Benefits Streamlined faster purification protocol No use of hazardous organic chemicals Automatable on the QIAcube Efficient removal of PCR inhibitors High sensitivity for downstream assay

Catalog Number:

51604

Price:

269

Category:

QIAamp Fast DNA Stool Mini Kit

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Structured Review

Qiagen qiaamp fast dna stool mini kit

qiaamp fast dna stool mini kit - by Bioz Stars, 2021-04

99/100 stars

Images

1) Product Images from "Detection of Clostridium botulinum group III in environmental samples from farms by real-time PCR using four commercial DNA extraction kits"

Article Title: Detection of Clostridium botulinum group III in environmental samples from farms by real-time PCR using four commercial DNA extraction kits

Journal: BMC Research Notes

doi: 10.1186/s13104-018-3549-5

Number of samples in which C. botulinum group III was detected using real-time PCR according to the DNA extraction kit used and the analyzed matrix. Total: number of samples analyzed per matrix. QA QIAamp ® DNA Mini Kit (QIAGEN Inc., Valencia, CA, USA), QF QIAamp ® Fast DNA Stool Mini Kit (QIAGEN Inc., Valencia, CA, USA), PS PowerSoil ® DNA isolation kit (Mo Bio Laboratories Inc., Carlsbad, CA, USA), NS NucleoSpin ® Soil (Macherey–Nagel, Duren, Germany)

Figure Legend Snippet: Number of samples in which C. botulinum group III was detected using real-time PCR according to the DNA extraction kit used and the analyzed matrix. Total: number of samples analyzed per matrix. QA QIAamp ® DNA Mini Kit (QIAGEN Inc., Valencia, CA, USA), QF QIAamp ® Fast DNA Stool Mini Kit (QIAGEN Inc., Valencia, CA, USA), PS PowerSoil ® DNA isolation kit (Mo Bio Laboratories Inc., Carlsbad, CA, USA), NS NucleoSpin ® Soil (Macherey–Nagel, Duren, Germany)

Techniques Used: Real-time Polymerase Chain Reaction, DNA Extraction

Modification:

Article Title: Biases from different DNA extraction methods in intestine microbiome research based on 16S rDNA sequencing: a case in the koi carp, Cyprinus carpio var. Koi. Biases from different DNA extraction methods in intestine microbiome research based on 16S rDNA sequencing: a case in the koi carp, Cyprinus carpio var. Koi
Article Snippet: .. Specifically, a protocol was modified from the lysozyme method developed by our laboratory and named the Combination of Lysozyme and Ultrasonic Lysis method (CLU); the Zirmil‐beating Cell Disruption method (ZBC) referring to the research of Zoetendal et al. ( ) and a QIAamp Fast DNA Stool Mini Kit (QIA, Qiagen, Hilden, Germany), a common commercial kit, were also used. .. Koi carp were provided by the Gongwang koi fish‐breeding center in Tianjin, China.

Lysis:

DNA Extraction:

Article Title: Detection of Clostridium botulinum group III in environmental samples from farms by real-time PCR using four commercial DNA extraction kits
Article Snippet: .. The following kits were evaluated: PowerSoil® DNA isolation kit (Mo Bio Laboratories Inc., Carlsbad, CA, USA), QIAamp® Fast DNA Stool Mini Kit and QIAamp® DNA Mini Kit (QIAGEN Inc., Valencia, CA, USA), and NucleoSpin® Soil (Macherey–Nagel, Duren, Germany). .. For NucleoSpin® Soil, sample lysis was performed with the optional enhancer SX solution and SL1 buffer.

Amplification:

Article Title: Klebsiella oxytoca expands in cancer cachexia and acts as a gut pathobiont contributing to intestinal dysfunction
Article Snippet: All isolates were identified by full length 16S rRNA gene sequencing, a method that has been validated for identification of Klebsiella . .. Briefly, DNA of these bacteria was extracted using the QI Amp Fast DNA stool Minikit (Qiagen, Germany) and amplified using 8 F and 1391 R primers (primer sequences in Table ). .. The PCR products were purified and subjected to sequencing using the services of DNAVision (Belgium) or Eurofins Genomics (Germany).