qiaamp dna stool minikit 51304  (Qiagen)


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    Name:
    QIAamp DNA Stool Mini Kit
    Description:
    The QIAamp Fast DNA Stool Mini Kit enables rapid purification of high quality genomic DNA human and bacterial from fresh or frozen stool samples The novel InhibitEX Buffer replaces cumbersome inhibitor removal tablets to efficiently remove PCR inhibitors commonly present in stool samples Fewer protocol steps streamlines your workflow and reduces hands on time during extraction The QIAamp Fast DNA Stool Mini Kit may be used with a microcentrifuge and may be automated on the QIAcube
    Catalog Number:
    51504
    Price:
    None
    Category:
    RNA DNA purification
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    Structured Review

    Qiagen qiaamp dna stool minikit 51304
    QIAamp DNA Stool Mini Kit
    The QIAamp Fast DNA Stool Mini Kit enables rapid purification of high quality genomic DNA human and bacterial from fresh or frozen stool samples The novel InhibitEX Buffer replaces cumbersome inhibitor removal tablets to efficiently remove PCR inhibitors commonly present in stool samples Fewer protocol steps streamlines your workflow and reduces hands on time during extraction The QIAamp Fast DNA Stool Mini Kit may be used with a microcentrifuge and may be automated on the QIAcube
    https://www.bioz.com/result/qiaamp dna stool minikit 51304/product/Qiagen
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna stool minikit 51304 - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Centrifugation:

    Article Title: A qPCR Assay to Detect and Quantify Shiga Toxin-Producing E. coli (STEC) in Cattle and on Farms: A Potential Predictive Tool for STEC Culture-Positive Farms
    Article Snippet: .. Subsequently, 2.5 mL volumes were concentrated by centrifugation (14,000 g, for 5 min) and the pellet (corresponding with 0.25 g feces) was subjected to DNA extraction using the QIAamp DNA Stool Mini Kit (Qiagen Inc, Valencia, CA, US) according to the manufacturer’s instructions. ..

    Amplification:

    Article Title: Stages of pregnancy and weaning influence the gut microbiota diversity and function in sows
    Article Snippet: The total microbial genomic DNA from faeces was extracted using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. The protocols of PCR amplification, gel extraction and sequencing library construction used in this study were described by Li et al. ( ).

    Article Title: The Positive Effects of Grifola frondosa Heteropolysaccharide on NAFLD and Regulation of the Gut Microbiota
    Article Snippet: Cecal DNA Extraction and High-Throughput Sequencing Cecal DNA was prepared using a QIAamp-DNA Stool Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocols. .. The DNA sequences of cecal contents were individually amplified with primer pairs targeting the 16S rRNA gene.

    Article Title: Microbiome of Co-cultured Fish Exhibits Host Selection and Niche Differentiation at the Organ Scale
    Article Snippet: .. DNA Extraction and 16S rRNA Gene PCR Amplification Genomic DNA was extracted from the fish and feed samples using QIAamp DNA Stool Mini Kit (Qiagen, Hilden, NRW, Germany) and from the water samples using Mo PowerWater DNA Isolation kits (MoBio, Valencia, CA, United States) following the manufacturer’s protocol with minor modifications to increase DNA yield, including resuspension in 100 μl TE buffer solution. .. The V4-V5 region of the bacteria was PCR-amplified with the bar coded-primers (515F, 5′-GTGCCAGCMGCCGCGGTAA-3′ and 907R, 5′-CCG TCAATTCCTTTGAGTTT-3′).

    Article Title: Exploring possible associations of the intestine bacterial microbiome with the pre-weaned weight gaining performance of piglets in intensive pig production
    Article Snippet: Sample processing and sequencing From each sample, DNA was extracted from 0.25 g of sample by using QIAamp DNA Stool Mini Kit according to the manufacturer’s instruction (QIAGEN, German). .. Phusion® High-Fidelity PCR Master Mix (ThermoFisher, United States) was used for two rounds PCR amplification.

    Article Title: Housing Systems Influence Gut Microbiota Composition of Sows but Not of Their Piglets
    Article Snippet: Following homogenization, the DNA was extracted using the QIAamp DNA Stool Mini Kit according to the manufacturer’s instructions (Qiagen). .. The sequences in italics served as an index and adapter ligation while underlined sequences allowed for amplification over V3/V4 region of eubacterial 16S rRNA genes.

    Article Title: Differential Effects of Typical Korean Versus American-Style Diets on Gut Microbial Composition and Metabolic Profile in Healthy Overweight Koreans: A Randomized Crossover Trial
    Article Snippet: 16S rRNA Gene Sequencing and Microbiome Data Analysis Total bacterial DNA was isolated from fecal samples using the QIAamp® DNA stool Mini Kit (QIAGEN, Hilden, Germany) according to the kit protocol for pathogen detection with a few modifications. .. Bacterial 16S rRNA gene amplicons were amplified with universal primers, which anneal to the hypervariable region V1–V2 of the bacterial 16S rRNA gene.

    SYBR Green Assay:

    Article Title: Plant-derived exosomal microRNAs shape the gut microbiota
    Article Snippet: For gut bacteria identification, qPCR was performed from gut microbiota-derived DNA extracted with a QIAamp DNA Stool Mini Kit (Qiagen). .. Quantitation was performed using SsoAdvancedTM Universal SYBR Green Supermix (BioRad), and the bacterial specific primers are listed in . qPCR was performed using the BioRad CFX96 qPCR System with each reaction run in triplicate.

    Quantitation Assay:

    Article Title: Plant-derived exosomal microRNAs shape the gut microbiota
    Article Snippet: For gut bacteria identification, qPCR was performed from gut microbiota-derived DNA extracted with a QIAamp DNA Stool Mini Kit (Qiagen). .. Quantitation was performed using SsoAdvancedTM Universal SYBR Green Supermix (BioRad), and the bacterial specific primers are listed in . qPCR was performed using the BioRad CFX96 qPCR System with each reaction run in triplicate.

    Sequencing:

    Article Title: Stages of pregnancy and weaning influence the gut microbiota diversity and function in sows
    Article Snippet: Paragraph title: DNA extraction and 16S rRNA gene sequencing ... The total microbial genomic DNA from faeces was extracted using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

    Article Title: The Positive Effects of Grifola frondosa Heteropolysaccharide on NAFLD and Regulation of the Gut Microbiota
    Article Snippet: .. Cecal DNA Extraction and High-Throughput Sequencing Cecal DNA was prepared using a QIAamp-DNA Stool Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocols. .. The DNA sequences of cecal contents were individually amplified with primer pairs targeting the 16S rRNA gene.

    Article Title: Exploring possible associations of the intestine bacterial microbiome with the pre-weaned weight gaining performance of piglets in intensive pig production
    Article Snippet: .. Sample processing and sequencing From each sample, DNA was extracted from 0.25 g of sample by using QIAamp DNA Stool Mini Kit according to the manufacturer’s instruction (QIAGEN, German). .. The extracted DNA concentration and purity was monitored on 1.2% agarose gel electrophoresis.

    Article Title: Dynamic microbe and molecule networks in a mouse model of colitis-associated colorectal cancer
    Article Snippet: .. 16S rRNA gene library construction and sequencing Genomic DNA was extracted and purified with the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) and used for the preparation of 16S rRNA amplicons. .. PCR primers targeting the variable V3 region were used to amplify the 16S rRNA (forward primer: 5′- CCTACGGGAGGCAGCAG-3′, reverse primer: 5′-ATTACCGCGGCTGCTGG-3′) .

    Article Title: Multiplex Real-Time PCR Assay Using Scorpion Probes and DNA Capture for Genotype-Specific Detection of Giardia lamblia on Fecal Samples
    Article Snippet: DNA was then extracted using a QIAamp DNA Stool Mini kit (QIAGEN, Valencia, Calif.) per the manufacturer's instructions except that the suspension was incubated in the kit's stool lysis buffer at 95°C and a 3-min incubation with InhibitEx tablets was performed. .. For sequence-specific DNA capture, fecal samples were processed according to the protocol of Shuber et al. ( ).

    Article Title: Differential Effects of Typical Korean Versus American-Style Diets on Gut Microbial Composition and Metabolic Profile in Healthy Overweight Koreans: A Randomized Crossover Trial
    Article Snippet: .. 16S rRNA Gene Sequencing and Microbiome Data Analysis Total bacterial DNA was isolated from fecal samples using the QIAamp® DNA stool Mini Kit (QIAGEN, Hilden, Germany) according to the kit protocol for pathogen detection with a few modifications. .. Bacterial 16S rRNA gene amplicons were amplified with universal primers, which anneal to the hypervariable region V1–V2 of the bacterial 16S rRNA gene.

    Ligation:

    Article Title: Housing Systems Influence Gut Microbiota Composition of Sows but Not of Their Piglets
    Article Snippet: Following homogenization, the DNA was extracted using the QIAamp DNA Stool Mini Kit according to the manufacturer’s instructions (Qiagen). .. The sequences in italics served as an index and adapter ligation while underlined sequences allowed for amplification over V3/V4 region of eubacterial 16S rRNA genes.

    Light Microscopy:

    Article Title: Coleus amboinicus (Lour.) leaves as a modulator of ruminal methanogenesis and biohydrogenation in vitro
    Article Snippet: The protozoa numbers were counted under a light microscope (Zeiss, type Primo Star no. 5, Jena, Germany) using a drop of a mixed buffered ruminal fluid (1 mL) and 4% formaline (6 mL). .. For bacterial quantification, total DNA from the buffered ruminal fluid was extracted using QIAamp DNA Stool mini kit (Qiagen GmbH, Hilden, Germany).

    Polymerase Chain Reaction:

    Article Title: Stages of pregnancy and weaning influence the gut microbiota diversity and function in sows
    Article Snippet: The total microbial genomic DNA from faeces was extracted using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. The protocols of PCR amplification, gel extraction and sequencing library construction used in this study were described by Li et al. ( ).

    Article Title: The Positive Effects of Grifola frondosa Heteropolysaccharide on NAFLD and Regulation of the Gut Microbiota
    Article Snippet: Cecal DNA Extraction and High-Throughput Sequencing Cecal DNA was prepared using a QIAamp-DNA Stool Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocols. .. The V3–V4 region of the 16S rRNA gene (V3–V4 hypervariable regions) was amplified by PCR using specific primers (forward primer: 5′-CCTACGGRRBGCASCAGKVRVGAAT-3′; reverse primer: 5′-GGACTACNVGGGTWTCTAATCC-3′).

    Article Title: Microbiome of Co-cultured Fish Exhibits Host Selection and Niche Differentiation at the Organ Scale
    Article Snippet: .. DNA Extraction and 16S rRNA Gene PCR Amplification Genomic DNA was extracted from the fish and feed samples using QIAamp DNA Stool Mini Kit (Qiagen, Hilden, NRW, Germany) and from the water samples using Mo PowerWater DNA Isolation kits (MoBio, Valencia, CA, United States) following the manufacturer’s protocol with minor modifications to increase DNA yield, including resuspension in 100 μl TE buffer solution. .. The V4-V5 region of the bacteria was PCR-amplified with the bar coded-primers (515F, 5′-GTGCCAGCMGCCGCGGTAA-3′ and 907R, 5′-CCG TCAATTCCTTTGAGTTT-3′).

    Article Title: Exploring possible associations of the intestine bacterial microbiome with the pre-weaned weight gaining performance of piglets in intensive pig production
    Article Snippet: Sample processing and sequencing From each sample, DNA was extracted from 0.25 g of sample by using QIAamp DNA Stool Mini Kit according to the manufacturer’s instruction (QIAGEN, German). .. Phusion® High-Fidelity PCR Master Mix (ThermoFisher, United States) was used for two rounds PCR amplification.

    Article Title: Housing Systems Influence Gut Microbiota Composition of Sows but Not of Their Piglets
    Article Snippet: Following homogenization, the DNA was extracted using the QIAamp DNA Stool Mini Kit according to the manufacturer’s instructions (Qiagen). .. Prior to PCR, DNA samples were diluted to 5 ng/μl and used as a template in PCR with forward primer 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG -MID-GT- CCTACGGGNGGCWGCAG -3′ and reverse primer 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG -MID-GT- GACTACHVGGGTATCTAATCC -3′ .

    Article Title: Dynamic microbe and molecule networks in a mouse model of colitis-associated colorectal cancer
    Article Snippet: 16S rRNA gene library construction and sequencing Genomic DNA was extracted and purified with the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) and used for the preparation of 16S rRNA amplicons. .. PCR primers targeting the variable V3 region were used to amplify the 16S rRNA (forward primer: 5′- CCTACGGGAGGCAGCAG-3′, reverse primer: 5′-ATTACCGCGGCTGCTGG-3′) .

    Article Title: Influence of Leptin and Adiponectin Supplementation on Intraepithelial Lymphocyte and Microbiota Composition in Suckling Rats
    Article Snippet: DNA Extraction Cecal DNA was extracted using the QIAamp DNA Stool Mini Kit (Qiagen), as previously described ( ). .. Sterile glass beads (sized 1 mm Ø) were added together with stool lysis buffer to the samples and cell lysis was performed for 4 min at 25 Hz using a TissueLyser (Qiagen), followed by a heating step at 95°C for 5 min. After lysis, DNA-damaging substances and PCR inhibitors were neutralized using an InhibitEX tablets (provided with the kit) and the DNA was purified on QIAamp Mini spin columns.

    DNA Extraction:

    Article Title: Stages of pregnancy and weaning influence the gut microbiota diversity and function in sows
    Article Snippet: Paragraph title: DNA extraction and 16S rRNA gene sequencing ... The total microbial genomic DNA from faeces was extracted using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

    Article Title: The Positive Effects of Grifola frondosa Heteropolysaccharide on NAFLD and Regulation of the Gut Microbiota
    Article Snippet: .. Cecal DNA Extraction and High-Throughput Sequencing Cecal DNA was prepared using a QIAamp-DNA Stool Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocols. .. The DNA sequences of cecal contents were individually amplified with primer pairs targeting the 16S rRNA gene.

    Article Title: A qPCR Assay to Detect and Quantify Shiga Toxin-Producing E. coli (STEC) in Cattle and on Farms: A Potential Predictive Tool for STEC Culture-Positive Farms
    Article Snippet: .. Subsequently, 2.5 mL volumes were concentrated by centrifugation (14,000 g, for 5 min) and the pellet (corresponding with 0.25 g feces) was subjected to DNA extraction using the QIAamp DNA Stool Mini Kit (Qiagen Inc, Valencia, CA, US) according to the manufacturer’s instructions. ..

    Article Title: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
    Article Snippet: In this study, both 140 and 35 μL silica were tested and the amount of extracted fecal DNA was compared to the amount of extracted fecal DNA using the QIAamp DNA Stool Mini Kit (Qiagen). .. As shown in Table , faecal DNA extraction using easyMag® with 140 and 35 μL silica yielded an average of 30.9 μg/mL (Standard Deviation (SD) 12.5) and 18.5 μg/mL (SD 18.4) DNA, respectively using NanoDrop®, while faecal samples purified using QIAamp DNA Stool Mini Kit (Qiagen) yielded an average of 32.9 μg/mL (SD 24.1) DNA (Table ).

    Article Title: Microbiome of Co-cultured Fish Exhibits Host Selection and Niche Differentiation at the Organ Scale
    Article Snippet: .. DNA Extraction and 16S rRNA Gene PCR Amplification Genomic DNA was extracted from the fish and feed samples using QIAamp DNA Stool Mini Kit (Qiagen, Hilden, NRW, Germany) and from the water samples using Mo PowerWater DNA Isolation kits (MoBio, Valencia, CA, United States) following the manufacturer’s protocol with minor modifications to increase DNA yield, including resuspension in 100 μl TE buffer solution. .. The V4-V5 region of the bacteria was PCR-amplified with the bar coded-primers (515F, 5′-GTGCCAGCMGCCGCGGTAA-3′ and 907R, 5′-CCG TCAATTCCTTTGAGTTT-3′).

    Article Title: Multiplex Real-Time PCR Assay Using Scorpion Probes and DNA Capture for Genotype-Specific Detection of Giardia lamblia on Fecal Samples
    Article Snippet: Paragraph title: DNA extraction. ... DNA was then extracted using a QIAamp DNA Stool Mini kit (QIAGEN, Valencia, Calif.) per the manufacturer's instructions except that the suspension was incubated in the kit's stool lysis buffer at 95°C and a 3-min incubation with InhibitEx tablets was performed.

    Article Title: Influence of Leptin and Adiponectin Supplementation on Intraepithelial Lymphocyte and Microbiota Composition in Suckling Rats
    Article Snippet: .. DNA Extraction Cecal DNA was extracted using the QIAamp DNA Stool Mini Kit (Qiagen), as previously described ( ). ..

    Article Title: Recombinant porcine norovirus identified from piglet with diarrhea
    Article Snippet: Paragraph title: RNA and DNA extraction ... DNA was extracted using The QIAamp DNA Stool Mini Kit (QIAGEN, Germany) according to the manufacturer’s instructions.

    Isolation:

    Article Title: A qPCR Assay to Detect and Quantify Shiga Toxin-Producing E. coli (STEC) in Cattle and on Farms: A Potential Predictive Tool for STEC Culture-Positive Farms
    Article Snippet: The other 59 samples were analyzed as native fecal samples using qPCR and classical culture for STEC isolation. .. Subsequently, 2.5 mL volumes were concentrated by centrifugation (14,000 g, for 5 min) and the pellet (corresponding with 0.25 g feces) was subjected to DNA extraction using the QIAamp DNA Stool Mini Kit (Qiagen Inc, Valencia, CA, US) according to the manufacturer’s instructions.

    Article Title: Differential Effects of Typical Korean Versus American-Style Diets on Gut Microbial Composition and Metabolic Profile in Healthy Overweight Koreans: A Randomized Crossover Trial
    Article Snippet: .. 16S rRNA Gene Sequencing and Microbiome Data Analysis Total bacterial DNA was isolated from fecal samples using the QIAamp® DNA stool Mini Kit (QIAGEN, Hilden, Germany) according to the kit protocol for pathogen detection with a few modifications. .. Bacterial 16S rRNA gene amplicons were amplified with universal primers, which anneal to the hypervariable region V1–V2 of the bacterial 16S rRNA gene.

    Purification:

    Article Title: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
    Article Snippet: In this study, both 140 and 35 μL silica were tested and the amount of extracted fecal DNA was compared to the amount of extracted fecal DNA using the QIAamp DNA Stool Mini Kit (Qiagen). .. As shown in Table , faecal DNA extraction using easyMag® with 140 and 35 μL silica yielded an average of 30.9 μg/mL (Standard Deviation (SD) 12.5) and 18.5 μg/mL (SD 18.4) DNA, respectively using NanoDrop®, while faecal samples purified using QIAamp DNA Stool Mini Kit (Qiagen) yielded an average of 32.9 μg/mL (SD 24.1) DNA (Table ).

    Article Title: Dynamic microbe and molecule networks in a mouse model of colitis-associated colorectal cancer
    Article Snippet: .. 16S rRNA gene library construction and sequencing Genomic DNA was extracted and purified with the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) and used for the preparation of 16S rRNA amplicons. .. PCR primers targeting the variable V3 region were used to amplify the 16S rRNA (forward primer: 5′- CCTACGGGAGGCAGCAG-3′, reverse primer: 5′-ATTACCGCGGCTGCTGG-3′) .

    Article Title: Influence of Leptin and Adiponectin Supplementation on Intraepithelial Lymphocyte and Microbiota Composition in Suckling Rats
    Article Snippet: DNA Extraction Cecal DNA was extracted using the QIAamp DNA Stool Mini Kit (Qiagen), as previously described ( ). .. Sterile glass beads (sized 1 mm Ø) were added together with stool lysis buffer to the samples and cell lysis was performed for 4 min at 25 Hz using a TissueLyser (Qiagen), followed by a heating step at 95°C for 5 min. After lysis, DNA-damaging substances and PCR inhibitors were neutralized using an InhibitEX tablets (provided with the kit) and the DNA was purified on QIAamp Mini spin columns.

    Standard Deviation:

    Article Title: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
    Article Snippet: In this study, both 140 and 35 μL silica were tested and the amount of extracted fecal DNA was compared to the amount of extracted fecal DNA using the QIAamp DNA Stool Mini Kit (Qiagen). .. As shown in Table , faecal DNA extraction using easyMag® with 140 and 35 μL silica yielded an average of 30.9 μg/mL (Standard Deviation (SD) 12.5) and 18.5 μg/mL (SD 18.4) DNA, respectively using NanoDrop®, while faecal samples purified using QIAamp DNA Stool Mini Kit (Qiagen) yielded an average of 32.9 μg/mL (SD 24.1) DNA (Table ).

    Gel Extraction:

    Article Title: Stages of pregnancy and weaning influence the gut microbiota diversity and function in sows
    Article Snippet: The total microbial genomic DNA from faeces was extracted using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. The protocols of PCR amplification, gel extraction and sequencing library construction used in this study were described by Li et al. ( ).

    Agarose Gel Electrophoresis:

    Article Title: Exploring possible associations of the intestine bacterial microbiome with the pre-weaned weight gaining performance of piglets in intensive pig production
    Article Snippet: Sample processing and sequencing From each sample, DNA was extracted from 0.25 g of sample by using QIAamp DNA Stool Mini Kit according to the manufacturer’s instruction (QIAGEN, German). .. The extracted DNA concentration and purity was monitored on 1.2% agarose gel electrophoresis.

    Real-time Polymerase Chain Reaction:

    Article Title: A qPCR Assay to Detect and Quantify Shiga Toxin-Producing E. coli (STEC) in Cattle and on Farms: A Potential Predictive Tool for STEC Culture-Positive Farms
    Article Snippet: The other 59 samples were analyzed as native fecal samples using qPCR and classical culture for STEC isolation. .. Subsequently, 2.5 mL volumes were concentrated by centrifugation (14,000 g, for 5 min) and the pellet (corresponding with 0.25 g feces) was subjected to DNA extraction using the QIAamp DNA Stool Mini Kit (Qiagen Inc, Valencia, CA, US) according to the manufacturer’s instructions.

    Article Title: Plant-derived exosomal microRNAs shape the gut microbiota
    Article Snippet: .. For gut bacteria identification, qPCR was performed from gut microbiota-derived DNA extracted with a QIAamp DNA Stool Mini Kit (Qiagen). .. Quantitation was performed using SsoAdvancedTM Universal SYBR Green Supermix (BioRad), and the bacterial specific primers are listed in . qPCR was performed using the BioRad CFX96 qPCR System with each reaction run in triplicate.

    Sample Prep:

    Article Title: A qPCR Assay to Detect and Quantify Shiga Toxin-Producing E. coli (STEC) in Cattle and on Farms: A Potential Predictive Tool for STEC Culture-Positive Farms
    Article Snippet: Paragraph title: Sample Preparation and DNA Extraction ... Subsequently, 2.5 mL volumes were concentrated by centrifugation (14,000 g, for 5 min) and the pellet (corresponding with 0.25 g feces) was subjected to DNA extraction using the QIAamp DNA Stool Mini Kit (Qiagen Inc, Valencia, CA, US) according to the manufacturer’s instructions.

    Homogenization:

    Article Title: Housing Systems Influence Gut Microbiota Composition of Sows but Not of Their Piglets
    Article Snippet: .. Following homogenization, the DNA was extracted using the QIAamp DNA Stool Mini Kit according to the manufacturer’s instructions (Qiagen). ..

    Incubation:

    Article Title: Stool antigen immunodetection for diagnosis of Giardia duodenalis infection in human subjects with HIV and cancer
    Article Snippet: .. After the addition of proteinase K, the suspensions were incubated at 56°C for 4–6 h, followed by gDNA extraction using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. The concentrations and the purity of extracted gDNA samples were measured at 260/280 nm, respectively using a NanoDrop-2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, USA).

    Article Title: Multiplex Real-Time PCR Assay Using Scorpion Probes and DNA Capture for Genotype-Specific Detection of Giardia lamblia on Fecal Samples
    Article Snippet: .. DNA was then extracted using a QIAamp DNA Stool Mini kit (QIAGEN, Valencia, Calif.) per the manufacturer's instructions except that the suspension was incubated in the kit's stool lysis buffer at 95°C and a 3-min incubation with InhibitEx tablets was performed. ..

    Spectrophotometry:

    Article Title: Stool antigen immunodetection for diagnosis of Giardia duodenalis infection in human subjects with HIV and cancer
    Article Snippet: After the addition of proteinase K, the suspensions were incubated at 56°C for 4–6 h, followed by gDNA extraction using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. The concentrations and the purity of extracted gDNA samples were measured at 260/280 nm, respectively using a NanoDrop-2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, USA).

    Article Title: Coleus amboinicus (Lour.) leaves as a modulator of ruminal methanogenesis and biohydrogenation in vitro
    Article Snippet: For bacterial quantification, total DNA from the buffered ruminal fluid was extracted using QIAamp DNA Stool mini kit (Qiagen GmbH, Hilden, Germany). .. DNA concentration was measured with a NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, USA).

    Concentration Assay:

    Article Title: Stages of pregnancy and weaning influence the gut microbiota diversity and function in sows
    Article Snippet: The total microbial genomic DNA from faeces was extracted using a QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. The DNA concentration of each sample was measured with a NanoDrop® ND‐1000 instrument (NanoDrop Technologies Inc., Waltham, MA).

    Article Title: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
    Article Snippet: In this study, both 140 and 35 μL silica were tested and the amount of extracted fecal DNA was compared to the amount of extracted fecal DNA using the QIAamp DNA Stool Mini Kit (Qiagen). .. When DNA extracts were measured with the Qubit® system, the average concentration of DNA was measured to be 0.8 μg/mL (SD 0.5), 1.8 μg/mL (SD 2.0) and 6.3 μg/mL (SD 9.4), for easyMag® with 140, 35 μL silica and Qiagen respectively (Table ).

    Article Title: Exploring possible associations of the intestine bacterial microbiome with the pre-weaned weight gaining performance of piglets in intensive pig production
    Article Snippet: Sample processing and sequencing From each sample, DNA was extracted from 0.25 g of sample by using QIAamp DNA Stool Mini Kit according to the manufacturer’s instruction (QIAGEN, German). .. The extracted DNA concentration and purity was monitored on 1.2% agarose gel electrophoresis.

    Article Title: Dynamic microbe and molecule networks in a mouse model of colitis-associated colorectal cancer
    Article Snippet: 16S rRNA gene library construction and sequencing Genomic DNA was extracted and purified with the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) and used for the preparation of 16S rRNA amplicons. .. Each reaction mixture contained 25 μL 2× PrimerStar HS Premix (Takara, Dalian, China), 0.5 μL each of the forward and reverse primers (10 μM final concentration), 50 ng template DNA, 0.2 mM dNTPs and 1 U PrimeSTAR HS DNA Polymerase (Takara, Dalian, China) in a final volume of 50 μl.

    Article Title: Coleus amboinicus (Lour.) leaves as a modulator of ruminal methanogenesis and biohydrogenation in vitro
    Article Snippet: For bacterial quantification, total DNA from the buffered ruminal fluid was extracted using QIAamp DNA Stool mini kit (Qiagen GmbH, Hilden, Germany). .. DNA concentration was measured with a NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, USA).

    High Throughput Screening Assay:

    Article Title: The Positive Effects of Grifola frondosa Heteropolysaccharide on NAFLD and Regulation of the Gut Microbiota
    Article Snippet: .. Cecal DNA Extraction and High-Throughput Sequencing Cecal DNA was prepared using a QIAamp-DNA Stool Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocols. .. The DNA sequences of cecal contents were individually amplified with primer pairs targeting the 16S rRNA gene.

    Lysis:

    Article Title: Multiplex Real-Time PCR Assay Using Scorpion Probes and DNA Capture for Genotype-Specific Detection of Giardia lamblia on Fecal Samples
    Article Snippet: .. DNA was then extracted using a QIAamp DNA Stool Mini kit (QIAGEN, Valencia, Calif.) per the manufacturer's instructions except that the suspension was incubated in the kit's stool lysis buffer at 95°C and a 3-min incubation with InhibitEx tablets was performed. ..

    Article Title: Influence of Leptin and Adiponectin Supplementation on Intraepithelial Lymphocyte and Microbiota Composition in Suckling Rats
    Article Snippet: DNA Extraction Cecal DNA was extracted using the QIAamp DNA Stool Mini Kit (Qiagen), as previously described ( ). .. Sterile glass beads (sized 1 mm Ø) were added together with stool lysis buffer to the samples and cell lysis was performed for 4 min at 25 Hz using a TissueLyser (Qiagen), followed by a heating step at 95°C for 5 min. After lysis, DNA-damaging substances and PCR inhibitors were neutralized using an InhibitEX tablets (provided with the kit) and the DNA was purified on QIAamp Mini spin columns.

    Fluorescence In Situ Hybridization:

    Article Title: Microbiome of Co-cultured Fish Exhibits Host Selection and Niche Differentiation at the Organ Scale
    Article Snippet: .. DNA Extraction and 16S rRNA Gene PCR Amplification Genomic DNA was extracted from the fish and feed samples using QIAamp DNA Stool Mini Kit (Qiagen, Hilden, NRW, Germany) and from the water samples using Mo PowerWater DNA Isolation kits (MoBio, Valencia, CA, United States) following the manufacturer’s protocol with minor modifications to increase DNA yield, including resuspension in 100 μl TE buffer solution. .. The V4-V5 region of the bacteria was PCR-amplified with the bar coded-primers (515F, 5′-GTGCCAGCMGCCGCGGTAA-3′ and 907R, 5′-CCG TCAATTCCTTTGAGTTT-3′).

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    Qiagen qiaamp dna mini kit
    Influence of proteinase K digestion (56 °C overnight) on <t>DNA</t> extraction. Graph A shows the Ct values obtained by quantifying THP1 cell DNA derived from direct extraction with the NucliSENS easyMAG system and extraction performed on the same quantity of cells following overnight (ON) digestion with Proteinase K. Graph B shows Leishmania quantification after extraction with the NucliSENS easyMAG system both with and without PK and quantification after extraction using a <t>QIAamp</t> DNA Mini kit after ON digestion with PK.
    Qiaamp Dna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 3670 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp dna mini kit/product/Qiagen
    Average 90 stars, based on 3670 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna mini kit - by Bioz Stars, 2020-01
    90/100 stars
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    Influence of proteinase K digestion (56 °C overnight) on DNA extraction. Graph A shows the Ct values obtained by quantifying THP1 cell DNA derived from direct extraction with the NucliSENS easyMAG system and extraction performed on the same quantity of cells following overnight (ON) digestion with Proteinase K. Graph B shows Leishmania quantification after extraction with the NucliSENS easyMAG system both with and without PK and quantification after extraction using a QIAamp DNA Mini kit after ON digestion with PK.

    Journal: Parasite

    Article Title: Application of the NucliSENS easyMAG system for nucleic acid extraction: optimization of DNA extraction for molecular diagnosis of parasitic and fungal diseases

    doi: 10.1051/parasite/2013051

    Figure Lengend Snippet: Influence of proteinase K digestion (56 °C overnight) on DNA extraction. Graph A shows the Ct values obtained by quantifying THP1 cell DNA derived from direct extraction with the NucliSENS easyMAG system and extraction performed on the same quantity of cells following overnight (ON) digestion with Proteinase K. Graph B shows Leishmania quantification after extraction with the NucliSENS easyMAG system both with and without PK and quantification after extraction using a QIAamp DNA Mini kit after ON digestion with PK.

    Article Snippet: 2.7 QIAamp DNA Mini kit We compared the DNA yield obtained from cell suspensions using the NucliSENS easyMAG system and the QIAamp DNA Mini kit (Qiagen ref. 51034).

    Techniques: DNA Extraction, Derivative Assay