eccdna enrichment  (Qiagen)


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    Name:
    QIAamp DNA Blood Maxi Kit
    Description:
    For purification of up to 600 µg genomic mitochondrial or viral DNA from blood Kit contents Qiagen QIAamp DNA Blood Maxi Kit 10 preps 3 to 10mL Sample 500 to 2000L Elution Volume Whole Blood Body Fluids Sample Silica Technology Manual Processing Spin Column Format 300 to 600g Yield Ideal for PCR Blotting Applications For Purification of Genomic Mitochondrial or Viral DNA from Blood Includes 10 QIAamp Maxi Spin Columns Qiagen Protease Buffers 50mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors
    Catalog Number:
    51192
    Price:
    210
    Category:
    QIAamp DNA Blood Maxi Kit
    Buy from Supplier


    Structured Review

    Qiagen eccdna enrichment
    QIAamp DNA Blood Maxi Kit
    For purification of up to 600 µg genomic mitochondrial or viral DNA from blood Kit contents Qiagen QIAamp DNA Blood Maxi Kit 10 preps 3 to 10mL Sample 500 to 2000L Elution Volume Whole Blood Body Fluids Sample Silica Technology Manual Processing Spin Column Format 300 to 600g Yield Ideal for PCR Blotting Applications For Purification of Genomic Mitochondrial or Viral DNA from Blood Includes 10 QIAamp Maxi Spin Columns Qiagen Protease Buffers 50mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors
    https://www.bioz.com/result/eccdna enrichment/product/Qiagen
    Average 92 stars, based on 4399 article reviews
    Price from $9.99 to $1999.99
    eccdna enrichment - by Bioz Stars, 2020-04
    92/100 stars

    Images

    1) Product Images from "Molecular characterization of cell-free eccDNAs in human plasma"

    Article Title: Molecular characterization of cell-free eccDNAs in human plasma

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-11368-w

    Flow chart of eccDNA preparation and sequencing data analysis. cfDNA was extracted from human plasma. Plasmid-Safe ATP-dependent DNase was used to remove the linear DNA. The DNase-resistant DNA (circular DNA) was further amplified using MDA method. The MDA products were sheared into 100–300 bp fragments, which were then used for library preparation and sequencing analyses. By removing mitochondrial sequence, the remaining sequences were aligned to human genome sequences. A split-read based method (Split-align) was used to determine the eccDNAs.
    Figure Legend Snippet: Flow chart of eccDNA preparation and sequencing data analysis. cfDNA was extracted from human plasma. Plasmid-Safe ATP-dependent DNase was used to remove the linear DNA. The DNase-resistant DNA (circular DNA) was further amplified using MDA method. The MDA products were sheared into 100–300 bp fragments, which were then used for library preparation and sequencing analyses. By removing mitochondrial sequence, the remaining sequences were aligned to human genome sequences. A split-read based method (Split-align) was used to determine the eccDNAs.

    Techniques Used: Flow Cytometry, Sequencing, Plasmid Preparation, Amplification, Multiple Displacement Amplification

    Effect of DNase digestion and initial DNA input on eccDNA detection. ( A ) Number of uniquely detected eccDNAs in different groups in samples 1 and 2. Compared to the digested groups, the undigested groups generated extremely low eccDNAs (3–9 for each assay). In the digested groups, eccDNA detection rate is reduced with the decreasing of initial cfDNA input. ( B ) Ratio between mapped mitochondria and genome sequence reads (MT/genome) in different groups in samples 1 and 2. The ratio of MT/genome decreased significantly in the undigested groups.
    Figure Legend Snippet: Effect of DNase digestion and initial DNA input on eccDNA detection. ( A ) Number of uniquely detected eccDNAs in different groups in samples 1 and 2. Compared to the digested groups, the undigested groups generated extremely low eccDNAs (3–9 for each assay). In the digested groups, eccDNA detection rate is reduced with the decreasing of initial cfDNA input. ( B ) Ratio between mapped mitochondria and genome sequence reads (MT/genome) in different groups in samples 1 and 2. The ratio of MT/genome decreased significantly in the undigested groups.

    Techniques Used: Generated, Sequencing

    2) Product Images from "Molecular characterization of cell-free eccDNAs in human plasma"

    Article Title: Molecular characterization of cell-free eccDNAs in human plasma

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-11368-w

    Flow chart of eccDNA preparation and sequencing data analysis. cfDNA was extracted from human plasma. Plasmid-Safe ATP-dependent DNase was used to remove the linear DNA. The DNase-resistant DNA (circular DNA) was further amplified using MDA method. The MDA products were sheared into 100–300 bp fragments, which were then used for library preparation and sequencing analyses. By removing mitochondrial sequence, the remaining sequences were aligned to human genome sequences. A split-read based method (Split-align) was used to determine the eccDNAs.
    Figure Legend Snippet: Flow chart of eccDNA preparation and sequencing data analysis. cfDNA was extracted from human plasma. Plasmid-Safe ATP-dependent DNase was used to remove the linear DNA. The DNase-resistant DNA (circular DNA) was further amplified using MDA method. The MDA products were sheared into 100–300 bp fragments, which were then used for library preparation and sequencing analyses. By removing mitochondrial sequence, the remaining sequences were aligned to human genome sequences. A split-read based method (Split-align) was used to determine the eccDNAs.

    Techniques Used: Flow Cytometry, Sequencing, Plasmid Preparation, Amplification, Multiple Displacement Amplification

    Effect of DNase digestion and initial DNA input on eccDNA detection. ( A ) Number of uniquely detected eccDNAs in different groups in samples 1 and 2. Compared to the digested groups, the undigested groups generated extremely low eccDNAs (3–9 for each assay). In the digested groups, eccDNA detection rate is reduced with the decreasing of initial cfDNA input. ( B ) Ratio between mapped mitochondria and genome sequence reads (MT/genome) in different groups in samples 1 and 2. The ratio of MT/genome decreased significantly in the undigested groups.
    Figure Legend Snippet: Effect of DNase digestion and initial DNA input on eccDNA detection. ( A ) Number of uniquely detected eccDNAs in different groups in samples 1 and 2. Compared to the digested groups, the undigested groups generated extremely low eccDNAs (3–9 for each assay). In the digested groups, eccDNA detection rate is reduced with the decreasing of initial cfDNA input. ( B ) Ratio between mapped mitochondria and genome sequence reads (MT/genome) in different groups in samples 1 and 2. The ratio of MT/genome decreased significantly in the undigested groups.

    Techniques Used: Generated, Sequencing

    Study design to characterize eccDNAs in human plasma. cfDNAs from three plasma samples were divided into four groups. Groups 1, 2 and 3 were subjected to the ATP-dependent DNase digestion and MDA procedures while Group 4 went directly to the MDA procedure. Each group has a technical replicate. Water was used as a negative control. A total of 26 libraries were prepared for DNA sequencing. Another four libraries from one additional plasma cfDNA sample were sequenced as a validation.
    Figure Legend Snippet: Study design to characterize eccDNAs in human plasma. cfDNAs from three plasma samples were divided into four groups. Groups 1, 2 and 3 were subjected to the ATP-dependent DNase digestion and MDA procedures while Group 4 went directly to the MDA procedure. Each group has a technical replicate. Water was used as a negative control. A total of 26 libraries were prepared for DNA sequencing. Another four libraries from one additional plasma cfDNA sample were sequenced as a validation.

    Techniques Used: Multiple Displacement Amplification, Negative Control, DNA Sequencing

    Related Articles

    Amplification:

    Article Title: Elevated C-reactive protein levels and ARMS2/HTRA1 gene variants in subjects without age-related macular degeneration
    Article Snippet: In brief, genomic DNAs were extracted from peripheral blood using a kit (QIAamp DNA Blood Maxi; Qiagen Inc., Tokyo, Japan). .. The DNA fragments, including the variants, were amplified using PCR.

    Article Title: Shared Desmosome Gene Findings in Early and Late Onset Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy
    Article Snippet: Each participant had genomic DNA extracted from leukocytes present in whole blood using QIAmp DNA blood maxi kits (Qiagen, Valencia, CA). .. Intronic PKP2 , DSG2 , DSC2 , DSP , and JUP primers flanking each exon were used in polymerase chain aeaction (PCR) amplification.

    Real-time Polymerase Chain Reaction:

    Article Title: A randomized, double-blind, placebo-controlled trial of resveratrol for Alzheimer disease
    Article Snippet: The Biomarker Core performed APOE genotyping using real-time PCR restriction fragment length polymorphism analysis. .. Genomic DNA from blood was extracted using QIAamp DNA blood maxi kit (Qiagen, Venlo, Netherlands) and APOE genotyping performed using Applied Biosystems (Foster City, CA) TaqMan SNP Genotyping Assay.

    Article Title: Neuropsychiatric manifestations among HIV-1 infected African patients receiving efavirenz-based cART with or without tuberculosis treatment containing rifampicin
    Article Snippet: Genomic DNA was isolated from peripheral blood leukocytes using QIAamp DNA Maxi Kit (QIAGEN GmbH. .. Genotyping for SNPs were done by real - time PCR using pre-developed Taqman assay reagents for allelic discrimination (Applied Biosystems Genotyping Assays).

    Quantitation Assay:

    Article Title: A Novel Admixture-Based Pharmacogenetic Approach to Refine Warfarin Dosing in Caribbean Hispanics
    Article Snippet: Whole blood samples were processed for genomic DNA extraction using a QIAamp DNA Blood Maxi Kit (spin protocol; QIAGEN Inc., Venlo, Limburg) according to manufacturer’s instructions. .. DNA quantification was performed by using the NanoDrop® Spectrophotometer (ThermoScientific, Wilmington, DE, US) and fluorescent staining of double-stranded DNA (PicoGreen® dsDNA Quantitation Kit, Molecular Probes, Eugene, OR, US).

    Transplantation Assay:

    Article Title: Characterization of the major histocompatibility complex locus association with Behçet’s disease in Iran
    Article Snippet: HLA-B class I typing Blood samples were drawn into EDTA tubes and stored at −20°C until genomic DNA was extracted using QIAamp® DNA Blood Maxi kits (QIAGEN, Hilden, Germany) or a salting out procedure and diluted in Tris-EDTA buffer. .. The assays were performed at the Lisbon Center for Blood and Transplantation (Lisboa, Portugal) with lot#11 of LABType RSSO1B reagents (One Lambda, Canoga Park, CA, USA) according to manufacturer instructions.

    Hybridization:

    Article Title: Characterization of the major histocompatibility complex locus association with Behçet’s disease in Iran
    Article Snippet: HLA-B class I typing Blood samples were drawn into EDTA tubes and stored at −20°C until genomic DNA was extracted using QIAamp® DNA Blood Maxi kits (QIAGEN, Hilden, Germany) or a salting out procedure and diluted in Tris-EDTA buffer. .. A random subset (681 BD cases and 414 controls) of the total dataset was typed for HLA-B with a hybridization bead-based array using reverse-sequence specific oligo-probes (RSSOP) detected with a Luminex X-100 (Luminex Corp., Austin, TX, USA).

    Concentration Assay:

    Article Title: Genetic variation in KLK2 and KLK3 is associated with levels of hK2 and PSA in seminal plasma and in serum in young men
    Article Snippet: Genomic DNA was prepared from peripheral leukocytes using a QIAamp DNA Maxi Kit (Qiagen). .. DNA concentrations were determined by PicoGreen™ DNA assay (Molecular Probes), and all samples were adjusted to the same DNA concentration.

    Biomarker Assay:

    Article Title: A randomized, double-blind, placebo-controlled trial of resveratrol for Alzheimer disease
    Article Snippet: The Biomarker Core performed APOE genotyping using real-time PCR restriction fragment length polymorphism analysis. .. Genomic DNA from blood was extracted using QIAamp DNA blood maxi kit (Qiagen, Venlo, Netherlands) and APOE genotyping performed using Applied Biosystems (Foster City, CA) TaqMan SNP Genotyping Assay.

    DNA Sequencing:

    Article Title: Association of the Hermansky-Pudlak syndrome type 4 (HPS4) gene variants with cognitive function in patients with schizophrenia and healthy subjects
    Article Snippet: In brief, we extracted genomic DNA from the peripheral blood samples with a QIAamp DNA Blood Maxi Kit (QIAGEN Inc., Valencia, CA). .. The other three SNPs (rs61276843, rs713998, and rs2014410) were genotyped with direct DNA sequencing of polymerase chain reaction products with specific primer pairs, as previously reported [ ].

    Polymerase Chain Reaction:

    Article Title: Elevated C-reactive protein levels and ARMS2/HTRA1 gene variants in subjects without age-related macular degeneration
    Article Snippet: Genotyping The genotypes of ARMS2/HTRA1 variants were determined using direct sequencing and/or PCR-based assays [ ]. .. In brief, genomic DNAs were extracted from peripheral blood using a kit (QIAamp DNA Blood Maxi; Qiagen Inc., Tokyo, Japan).

    Article Title: Association of the Hermansky-Pudlak syndrome type 4 (HPS4) gene variants with cognitive function in patients with schizophrenia and healthy subjects
    Article Snippet: In brief, we extracted genomic DNA from the peripheral blood samples with a QIAamp DNA Blood Maxi Kit (QIAGEN Inc., Valencia, CA). .. The other three SNPs (rs61276843, rs713998, and rs2014410) were genotyped with direct DNA sequencing of polymerase chain reaction products with specific primer pairs, as previously reported [ ].

    Article Title: Shared Desmosome Gene Findings in Early and Late Onset Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy
    Article Snippet: Each participant had genomic DNA extracted from leukocytes present in whole blood using QIAmp DNA blood maxi kits (Qiagen, Valencia, CA). .. Intronic PKP2 , DSG2 , DSC2 , DSP , and JUP primers flanking each exon were used in polymerase chain aeaction (PCR) amplification.

    Article Title: Biological interaction of cigarette smoking on the association between genetic polymorphisms involved in inflammation and the risk of lung cancer: A case-control study in Japan
    Article Snippet: Genomic DNA was extracted from blood samples using the QIAmp DNA blood Maxi kit (Qiagen Inc., Valencia, CA, USA; #51192). .. Genotyping of the NF κ B1 -94 ATTG insertion/deletion polymorphism (rs28362491, II; insertion/insertion, ID; insertion/deletion, DD; deletion/deletion) was performed using the polymerase chain reaction restriction fragment-length polymorphism (PCR-RFLP) method previously described by Senol Tuncay et al ( ).

    Software:

    Article Title: Genetic variation in KLK2 and KLK3 is associated with levels of hK2 and PSA in seminal plasma and in serum in young men
    Article Snippet: Genomic DNA was prepared from peripheral leukocytes using a QIAamp DNA Maxi Kit (Qiagen). .. Genotypes were determined by Sequenom MassARRAY MALDI-TOF analysis and assay design was made using MassARRAY Assay Design 2.0 software (Sequenom) as previously described ( ).

    Article Title: Shared Desmosome Gene Findings in Early and Late Onset Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy
    Article Snippet: Each participant had genomic DNA extracted from leukocytes present in whole blood using QIAmp DNA blood maxi kits (Qiagen, Valencia, CA). .. The resultant PCR products were purified using the QIAquick PCR Purificatin Kit (Qiagen) followed by bidirectional sequence analysis using the ABI 3730 DNA Analyzer (Applied Biosystems, Foster City, CA) and chromatograms analyzed with Sequencer 4.1 software.

    DNA Extraction:

    Article Title: Adaptive Evolution of Toll-Like Receptors (TLRs) in the Family Suidae
    Article Snippet: Paragraph title: DNA extraction and sequencing ... Briefly, DNA was extracted from whole blood by using the QIAamp DNA blood spin kit (Qiagen Sciences) and quantity and quality parameters were performed on the Qubit 2.0 fluorometer (Invitrogen) and run on a 1% agarose gel.

    Article Title: Association of the Hermansky-Pudlak syndrome type 4 (HPS4) gene variants with cognitive function in patients with schizophrenia and healthy subjects
    Article Snippet: Genomic DNA extraction and genotyping from the peripheral blood samples that were collected from the total subjects were performed as previously described [ ]. .. In brief, we extracted genomic DNA from the peripheral blood samples with a QIAamp DNA Blood Maxi Kit (QIAGEN Inc., Valencia, CA).

    Article Title: A Novel Admixture-Based Pharmacogenetic Approach to Refine Warfarin Dosing in Caribbean Hispanics
    Article Snippet: .. Whole blood samples were processed for genomic DNA extraction using a QIAamp DNA Blood Maxi Kit (spin protocol; QIAGEN Inc., Venlo, Limburg) according to manufacturer’s instructions. .. DNA quantification was performed by using the NanoDrop® Spectrophotometer (ThermoScientific, Wilmington, DE, US) and fluorescent staining of double-stranded DNA (PicoGreen® dsDNA Quantitation Kit, Molecular Probes, Eugene, OR, US).

    Mutagenesis:

    Article Title: Shared Desmosome Gene Findings in Early and Late Onset Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy
    Article Snippet: Paragraph title: Mutation Analysis ... Each participant had genomic DNA extracted from leukocytes present in whole blood using QIAmp DNA blood maxi kits (Qiagen, Valencia, CA).

    Article Title: A Novel Admixture-Based Pharmacogenetic Approach to Refine Warfarin Dosing in Caribbean Hispanics
    Article Snippet: Whole blood samples were processed for genomic DNA extraction using a QIAamp DNA Blood Maxi Kit (spin protocol; QIAGEN Inc., Venlo, Limburg) according to manufacturer’s instructions. .. Isolated DNA specimens were first tested for relevant polymorphisms on candidate warfarin-related pharmacogenes using the DMETTM plus assay (Affymetrix®, Santa Clara, CA, USA) at the RCMI Core Lab of Molecular Genetics (UPR-MSC), and confirmed by either pre-developed TaqMan® Genotyping Assay Reagent kits for Allelic Discrimination (Applied Biosystems, CA, USA) or Tag-It™ Mutation Detection Assay for warfarin (LUMINEX xMAP® Technology) at the CLIA-certified Laboratory of Personalized Health (Genomas Inc., Hartford, CT).

    Isolation:

    Article Title: DNA methylation and socioeconomic status in a Mexican-American birth cohort
    Article Snippet: .. DNA were isolated from clots with a QIAamp Blood DNA Maxi kit (Qiagen, Inc., Santa Clarita, CA, USA) (further details described in Holland et al. 2006) [ ]. .. For bisulfite conversion of 500 ng of DNA, we used EpiTect Bisulfite Conversion Kits (Qiagen, Germantown, MD, USA).

    Article Title: Neuropsychiatric manifestations among HIV-1 infected African patients receiving efavirenz-based cART with or without tuberculosis treatment containing rifampicin
    Article Snippet: .. Genomic DNA was isolated from peripheral blood leukocytes using QIAamp DNA Maxi Kit (QIAGEN GmbH. ..

    Article Title: A Novel Admixture-Based Pharmacogenetic Approach to Refine Warfarin Dosing in Caribbean Hispanics
    Article Snippet: Whole blood samples were processed for genomic DNA extraction using a QIAamp DNA Blood Maxi Kit (spin protocol; QIAGEN Inc., Venlo, Limburg) according to manufacturer’s instructions. .. Isolated DNA specimens were first tested for relevant polymorphisms on candidate warfarin-related pharmacogenes using the DMETTM plus assay (Affymetrix®, Santa Clara, CA, USA) at the RCMI Core Lab of Molecular Genetics (UPR-MSC), and confirmed by either pre-developed TaqMan® Genotyping Assay Reagent kits for Allelic Discrimination (Applied Biosystems, CA, USA) or Tag-It™ Mutation Detection Assay for warfarin (LUMINEX xMAP® Technology) at the CLIA-certified Laboratory of Personalized Health (Genomas Inc., Hartford, CT).

    Detection Assay:

    Article Title: A Novel Admixture-Based Pharmacogenetic Approach to Refine Warfarin Dosing in Caribbean Hispanics
    Article Snippet: Whole blood samples were processed for genomic DNA extraction using a QIAamp DNA Blood Maxi Kit (spin protocol; QIAGEN Inc., Venlo, Limburg) according to manufacturer’s instructions. .. Isolated DNA specimens were first tested for relevant polymorphisms on candidate warfarin-related pharmacogenes using the DMETTM plus assay (Affymetrix®, Santa Clara, CA, USA) at the RCMI Core Lab of Molecular Genetics (UPR-MSC), and confirmed by either pre-developed TaqMan® Genotyping Assay Reagent kits for Allelic Discrimination (Applied Biosystems, CA, USA) or Tag-It™ Mutation Detection Assay for warfarin (LUMINEX xMAP® Technology) at the CLIA-certified Laboratory of Personalized Health (Genomas Inc., Hartford, CT).

    Purification:

    Article Title: Shared Desmosome Gene Findings in Early and Late Onset Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy
    Article Snippet: Each participant had genomic DNA extracted from leukocytes present in whole blood using QIAmp DNA blood maxi kits (Qiagen, Valencia, CA). .. The resultant PCR products were purified using the QIAquick PCR Purificatin Kit (Qiagen) followed by bidirectional sequence analysis using the ABI 3730 DNA Analyzer (Applied Biosystems, Foster City, CA) and chromatograms analyzed with Sequencer 4.1 software.

    Sequencing:

    Article Title: A novel locus for X-linked congenital cataract on Xq24
    Article Snippet: DNA was extracted from whole blood using the QIAamp DNA blood maxi kit (Qiagen Pty Ltd, Doncaster, VIC, Australia) from all 22 available family members. .. A novel marker, CA-AC004000, was obtained by searching the reference sequence of the clone, AC004000, for CA repeats.

    Article Title: Adaptive Evolution of Toll-Like Receptors (TLRs) in the Family Suidae
    Article Snippet: Paragraph title: DNA extraction and sequencing ... Briefly, DNA was extracted from whole blood by using the QIAamp DNA blood spin kit (Qiagen Sciences) and quantity and quality parameters were performed on the Qubit 2.0 fluorometer (Invitrogen) and run on a 1% agarose gel.

    Article Title: Elevated C-reactive protein levels and ARMS2/HTRA1 gene variants in subjects without age-related macular degeneration
    Article Snippet: Genotyping The genotypes of ARMS2/HTRA1 variants were determined using direct sequencing and/or PCR-based assays [ ]. .. In brief, genomic DNAs were extracted from peripheral blood using a kit (QIAamp DNA Blood Maxi; Qiagen Inc., Tokyo, Japan).

    Article Title: Shared Desmosome Gene Findings in Early and Late Onset Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy
    Article Snippet: Each participant had genomic DNA extracted from leukocytes present in whole blood using QIAmp DNA blood maxi kits (Qiagen, Valencia, CA). .. The resultant PCR products were purified using the QIAquick PCR Purificatin Kit (Qiagen) followed by bidirectional sequence analysis using the ABI 3730 DNA Analyzer (Applied Biosystems, Foster City, CA) and chromatograms analyzed with Sequencer 4.1 software.

    Salting Out:

    Article Title: Characterization of the major histocompatibility complex locus association with Behçet’s disease in Iran
    Article Snippet: .. HLA-B class I typing Blood samples were drawn into EDTA tubes and stored at −20°C until genomic DNA was extracted using QIAamp® DNA Blood Maxi kits (QIAGEN, Hilden, Germany) or a salting out procedure and diluted in Tris-EDTA buffer. ..

    TaqMan Assay:

    Article Title: Association of the Hermansky-Pudlak syndrome type 4 (HPS4) gene variants with cognitive function in patients with schizophrenia and healthy subjects
    Article Snippet: In brief, we extracted genomic DNA from the peripheral blood samples with a QIAamp DNA Blood Maxi Kit (QIAGEN Inc., Valencia, CA). .. Two SNPs (rs4822724 and rs9608491) were genotyped with a TaqMan® assay (Assay ID: C___2490761_10 and C__11747810_10, respectively, Applied Biosystems, Foster City, CA) with a TP800 Dice Real Time System (TaKaRa Bio Inc., Otsu, Japan).

    Article Title: Neuropsychiatric manifestations among HIV-1 infected African patients receiving efavirenz-based cART with or without tuberculosis treatment containing rifampicin
    Article Snippet: Genomic DNA was isolated from peripheral blood leukocytes using QIAamp DNA Maxi Kit (QIAGEN GmbH. .. Genotyping for SNPs were done by real - time PCR using pre-developed Taqman assay reagents for allelic discrimination (Applied Biosystems Genotyping Assays).

    Selection:

    Article Title: Biological interaction of cigarette smoking on the association between genetic polymorphisms involved in inflammation and the risk of lung cancer: A case-control study in Japan
    Article Snippet: Genomic DNA was extracted from blood samples using the QIAmp DNA blood Maxi kit (Qiagen Inc., Valencia, CA, USA; #51192). .. Generally, the concordance rate between PCR-RFLP genotyping and reverse transcription-quantitative PCR assays is considered to be high ( ); however, for quality control, the two assays were repeated on a random selection of 5% of all samples, resulting in 100% concordance for the replicates.

    Agarose Gel Electrophoresis:

    Article Title: Adaptive Evolution of Toll-Like Receptors (TLRs) in the Family Suidae
    Article Snippet: .. Briefly, DNA was extracted from whole blood by using the QIAamp DNA blood spin kit (Qiagen Sciences) and quantity and quality parameters were performed on the Qubit 2.0 fluorometer (Invitrogen) and run on a 1% agarose gel. .. Library construction and re-sequencing of individual members of the family Suidae were done with 1–3 ug of genomic DNA according to the Illumina library prepping protocols.

    Article Title: Elevated C-reactive protein levels and ARMS2/HTRA1 gene variants in subjects without age-related macular degeneration
    Article Snippet: In brief, genomic DNAs were extracted from peripheral blood using a kit (QIAamp DNA Blood Maxi; Qiagen Inc., Tokyo, Japan). .. The genotypes of the del/ins variants in the ARMS2/HTRA1 locus were studied based on the size of the PCR-amplified DNA fragments visualized in agarose gel electrophoresis and/or by direct sequencing, as described above.

    Spectrophotometry:

    Article Title: A Novel Admixture-Based Pharmacogenetic Approach to Refine Warfarin Dosing in Caribbean Hispanics
    Article Snippet: Whole blood samples were processed for genomic DNA extraction using a QIAamp DNA Blood Maxi Kit (spin protocol; QIAGEN Inc., Venlo, Limburg) according to manufacturer’s instructions. .. DNA quantification was performed by using the NanoDrop® Spectrophotometer (ThermoScientific, Wilmington, DE, US) and fluorescent staining of double-stranded DNA (PicoGreen® dsDNA Quantitation Kit, Molecular Probes, Eugene, OR, US).

    DNA Methylation Assay:

    Article Title: DNA methylation and socioeconomic status in a Mexican-American birth cohort
    Article Snippet: Paragraph title: DNA methylation in cord blood ... DNA were isolated from clots with a QIAamp Blood DNA Maxi kit (Qiagen, Inc., Santa Clarita, CA, USA) (further details described in Holland et al. 2006) [ ].

    Genotyping Assay:

    Article Title: A Novel Admixture-Based Pharmacogenetic Approach to Refine Warfarin Dosing in Caribbean Hispanics
    Article Snippet: Whole blood samples were processed for genomic DNA extraction using a QIAamp DNA Blood Maxi Kit (spin protocol; QIAGEN Inc., Venlo, Limburg) according to manufacturer’s instructions. .. Isolated DNA specimens were first tested for relevant polymorphisms on candidate warfarin-related pharmacogenes using the DMETTM plus assay (Affymetrix®, Santa Clara, CA, USA) at the RCMI Core Lab of Molecular Genetics (UPR-MSC), and confirmed by either pre-developed TaqMan® Genotyping Assay Reagent kits for Allelic Discrimination (Applied Biosystems, CA, USA) or Tag-It™ Mutation Detection Assay for warfarin (LUMINEX xMAP® Technology) at the CLIA-certified Laboratory of Personalized Health (Genomas Inc., Hartford, CT).

    Marker:

    Article Title: A novel locus for X-linked congenital cataract on Xq24
    Article Snippet: DNA was extracted from whole blood using the QIAamp DNA blood maxi kit (Qiagen Pty Ltd, Doncaster, VIC, Australia) from all 22 available family members. .. A novel marker, CA-AC004000, was obtained by searching the reference sequence of the clone, AC004000, for CA repeats.

    Staining:

    Article Title: A Novel Admixture-Based Pharmacogenetic Approach to Refine Warfarin Dosing in Caribbean Hispanics
    Article Snippet: Whole blood samples were processed for genomic DNA extraction using a QIAamp DNA Blood Maxi Kit (spin protocol; QIAGEN Inc., Venlo, Limburg) according to manufacturer’s instructions. .. DNA quantification was performed by using the NanoDrop® Spectrophotometer (ThermoScientific, Wilmington, DE, US) and fluorescent staining of double-stranded DNA (PicoGreen® dsDNA Quantitation Kit, Molecular Probes, Eugene, OR, US).

    TaqMan SNP Genotyping Assay:

    Article Title: A randomized, double-blind, placebo-controlled trial of resveratrol for Alzheimer disease
    Article Snippet: .. Genomic DNA from blood was extracted using QIAamp DNA blood maxi kit (Qiagen, Venlo, Netherlands) and APOE genotyping performed using Applied Biosystems (Foster City, CA) TaqMan SNP Genotyping Assay. .. The assay was run on a Bio-Rad (Hercules, CA) CFX96.

    Luminex:

    Article Title: Characterization of the major histocompatibility complex locus association with Behçet’s disease in Iran
    Article Snippet: HLA-B class I typing Blood samples were drawn into EDTA tubes and stored at −20°C until genomic DNA was extracted using QIAamp® DNA Blood Maxi kits (QIAGEN, Hilden, Germany) or a salting out procedure and diluted in Tris-EDTA buffer. .. A random subset (681 BD cases and 414 controls) of the total dataset was typed for HLA-B with a hybridization bead-based array using reverse-sequence specific oligo-probes (RSSOP) detected with a Luminex X-100 (Luminex Corp., Austin, TX, USA).

    Article Title: A Novel Admixture-Based Pharmacogenetic Approach to Refine Warfarin Dosing in Caribbean Hispanics
    Article Snippet: Whole blood samples were processed for genomic DNA extraction using a QIAamp DNA Blood Maxi Kit (spin protocol; QIAGEN Inc., Venlo, Limburg) according to manufacturer’s instructions. .. Isolated DNA specimens were first tested for relevant polymorphisms on candidate warfarin-related pharmacogenes using the DMETTM plus assay (Affymetrix®, Santa Clara, CA, USA) at the RCMI Core Lab of Molecular Genetics (UPR-MSC), and confirmed by either pre-developed TaqMan® Genotyping Assay Reagent kits for Allelic Discrimination (Applied Biosystems, CA, USA) or Tag-It™ Mutation Detection Assay for warfarin (LUMINEX xMAP® Technology) at the CLIA-certified Laboratory of Personalized Health (Genomas Inc., Hartford, CT).

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    Qiagen qiaamp dna maxi kit
    Qiaamp Dna Maxi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 71 article reviews
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