qiaamp dna blood mini kit 51104  (Qiagen)


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    Name:
    QIAamp DNA Blood Mini Kit
    Description:
    For purification of up to 12 µg genomic mitochondrial or viral DNA from blood and related body fluids Kit contents Qiagen QIAamp DNA Blood Mini Kit 50 preps 1 to 200L Sample 50 to 200L Elution Volume Whole Blood Body Fluids Sample Spin Column Format Silica Technology Manual Processing 20 to 40 min Time Run 4 to 12g Yield For Purification of Up to 12g Genomic Mitochondrial or Viral DNA from Blood and Related Body Fluids Includes 50 QIAamp Mini Spin Columns Qiagen Protease Reagents Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors for reliable results
    Catalog Number:
    51104
    Price:
    157
    Category:
    QIAamp DNA Blood Mini Kit
    Buy from Supplier


    Structured Review

    Qiagen qiaamp dna blood mini kit 51104
    QIAamp DNA Blood Mini Kit
    For purification of up to 12 µg genomic mitochondrial or viral DNA from blood and related body fluids Kit contents Qiagen QIAamp DNA Blood Mini Kit 50 preps 1 to 200L Sample 50 to 200L Elution Volume Whole Blood Body Fluids Sample Spin Column Format Silica Technology Manual Processing 20 to 40 min Time Run 4 to 12g Yield For Purification of Up to 12g Genomic Mitochondrial or Viral DNA from Blood and Related Body Fluids Includes 50 QIAamp Mini Spin Columns Qiagen Protease Reagents Buffers 2mL Collection Tubes Benefits Rapid purification of high quality ready to use DNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors for reliable results
    https://www.bioz.com/result/qiaamp dna blood mini kit 51104/product/Qiagen
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna blood mini kit 51104 - by Bioz Stars, 2020-01
    90/100 stars

    Related Products / Commonly Used Together

    gdna
    pten mutation
    blood leukocytes
    genomic dna

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    Related Articles

    Clone Assay:

    Article Title: Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman‐ARMS Followed a Strict Control System
    Article Snippet: HBV viral DNA was isolated from 200 μl patient serum samples using the QIAamp DNA blood mini kit (Qiagen Inc., Basel, Switzerland) and eluted into 50 μl buffer according to manufacturer's instructions. .. Full‐length HBV genome was cloned according to the method of Parekh et al. , .

    Amplification:

    Article Title: Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China
    Article Snippet: Paragraph title: HBV DNA preparation and amplification ... Viral DNA was extracted from 200 μL of serum samples by using a QIAamp DNA Blood Mini kit (Qiagen Inc., Germany).

    Article Title: The Efficacy and Safety of Tyrosine Kinase Inhibitors for Von Hippel–Lindau Disease: A Retrospective Study of 32 Patients
    Article Snippet: Examination of VHL Mutation Genomic DNA was extracted from peripheral blood of suspected individuals using QIAamp DNA Blood Mini Kit (QIAGEN, Germany) according to instructions. .. Three coding exons and flanking intronic regions were amplified by polymerase chain reaction using primers as described in our previous publication ( , ).

    Article Title: Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman‐ARMS Followed a Strict Control System
    Article Snippet: In this study, we developed Taqman amplification refractory mutation system (Taqman‐ARMS) to real time detect G1896A mutant. .. HBV viral DNA was isolated from 200 μl patient serum samples using the QIAamp DNA blood mini kit (Qiagen Inc., Basel, Switzerland) and eluted into 50 μl buffer according to manufacturer's instructions.

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. Using ZiFit targeter software[ , ] and Cas-OFFinder [ ] to detect potential off-target sites, eight sets of primers were designed to amplify the 8-potential off-target sites ( ) from the genomic DNA using the online Primer Quest tool (Integrated DNA Technologies) based on available sequences obtained from the NCBI GenBank database and double checked by in-silico PCR amplification using NCBI primer blast tool.

    Article Title: Vitamin D deficiency in head and neck cancer patients – prevalence, prognostic value and impact on immune function
    Article Snippet: For the detection of HPV-DNA, DNA was extracted from fresh-frozen tumor tissue using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. PCR amplification products were detected with SYBR Green as well as gel electrophoresis.

    Article Title: Molecular genetics of late onset glycogen storage disease II in Italy
    Article Snippet: Genomic DNA was extracted with the QIAamp DNA blood Mini Kit (Qiagen GmbH, Hilden, Germany). .. GAA gene was amplified as described ( ).

    Construct:

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: Paragraph title: Insertion construct design and P. berghei transfection ... Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen).

    SYBR Green Assay:

    Article Title: Vitamin D deficiency in head and neck cancer patients – prevalence, prognostic value and impact on immune function
    Article Snippet: For the detection of HPV-DNA, DNA was extracted from fresh-frozen tumor tissue using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. PCR amplification products were detected with SYBR Green as well as gel electrophoresis.

    Microarray:

    Article Title: Chromosomal Aberrations in Pediatric Patients with Developmental Delay/Intellectual Disability: A Single-Center Clinical Investigation
    Article Snippet: .. Chromosomal Microarray Analysis Whole genomic DNA was extracted from peripheral blood cells of each patient and his or her parents using QIAamp DNA Blood Mini Kit (Qiagen, Valencia, CA, USA) and subjected to CMA-single nucleotide polymorphism (SNP) array analysis by using the Affymetrix® CytoScan™ 750K Array (Affymetrix, Santa Clara, CA, USA). .. When the fragment size of absence of heterozygosity (AOH) was larger than one-third of the chromosome, analysis software UPD tool_0.2 was used to separate the AOH into uniparental disomy (UPD) or consanguinity by comparison with the parental results.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Vitamin D deficiency in head and neck cancer patients – prevalence, prognostic value and impact on immune function
    Article Snippet: HPV-DNA-PCR was performed using fresh-frozen tumor tissue, whereas the p16-Ki67 dual staining was performed on FFPE tumor tissue samples. .. For the detection of HPV-DNA, DNA was extracted from fresh-frozen tumor tissue using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: Capturing intra-tumor genetic heterogeneity by de novomutation profiling of circulating cell-free tumor DNA: a proof-of-principle
    Article Snippet: .. DNA from tumor samples was extracted using the RecoverAll™ Total Nucleic Acid Isolation Kit (Ambion, Austin, TX) for FFPE tissue, and germline DNA was extracted from peripheral blood leukocytes using the QIAamp DNA Blood Mini Kit (Qiagen, Valencia, CA), according to the manufacturers' instructions. .. Plasma-derived DNA was extracted with the QIAamp Circulating Nucleic Acid Kit (Qiagen), as per the manufacturer's protocol.

    In Silico:

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. Using ZiFit targeter software[ , ] and Cas-OFFinder [ ] to detect potential off-target sites, eight sets of primers were designed to amplify the 8-potential off-target sites ( ) from the genomic DNA using the online Primer Quest tool (Integrated DNA Technologies) based on available sequences obtained from the NCBI GenBank database and double checked by in-silico PCR amplification using NCBI primer blast tool.

    Expressing:

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: The gene fragment was inserted into the pDb.Dh.^Db vector upstream of a selectable marker expressing the pyramethamine-resistant human dihydrofolate reductase (hDHFR) enzyme [ ]. .. Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen).

    Modification:

    Article Title: Application of a methylation gene panel by quantitative PCR for lung cancers
    Article Snippet: The modified DNA was used as a template for qPCR analysis. .. Genomic DNA was extracted from sputum samples by using the QIAamp DNA blood mini kit (QIAgen, Venlo, the Netherlands).

    Hybridization:

    Article Title: Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China
    Article Snippet: Viral DNA was extracted from 200 μL of serum samples by using a QIAamp DNA Blood Mini kit (Qiagen Inc., Germany). .. The target DNA for hybridization was amplified by a nested PCR.

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen). .. Resistant parasite populations were assayed by Southern hybridization and PCR to check for gene disruption at the targeted locus.

    High Performance Liquid Chromatography:

    Article Title: Molecular genetics of late onset glycogen storage disease II in Italy
    Article Snippet: Genomic DNA was extracted with the QIAamp DNA blood Mini Kit (Qiagen GmbH, Hilden, Germany). .. PCR products were screened by denaturing High Performance Liquid Chromatography (dHPLC, Varian, Palo Alto, CA, USA) and in the presence of heteroduplex, sequenced on the ABI PRISM 3700 DNA Analyzer.

    Transfection:

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: Paragraph title: Insertion construct design and P. berghei transfection ... Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen).

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: .. Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. DNA concentration and purity at the absorbance ratio 260/280 and 260/230 were determined on a NanoDrop 2000c Spectrophotometer (Thermo-Fisher Scientific).

    Ligation:

    Article Title: The Efficacy and Safety of Tyrosine Kinase Inhibitors for Von Hippel–Lindau Disease: A Retrospective Study of 32 Patients
    Article Snippet: Examination of VHL Mutation Genomic DNA was extracted from peripheral blood of suspected individuals using QIAamp DNA Blood Mini Kit (QIAGEN, Germany) according to instructions. .. Large deletions and duplications were detected by multiplex ligation-dependent probe amplification (MLPA, P016-C2 kit, MRC-Holland, Amsterdam, the Netherlands).

    Infection:

    Article Title: Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman‐ARMS Followed a Strict Control System
    Article Snippet: So it is very important to accurately detect HBV infection with G1896A mutant. .. HBV viral DNA was isolated from 200 μl patient serum samples using the QIAamp DNA blood mini kit (Qiagen Inc., Basel, Switzerland) and eluted into 50 μl buffer according to manufacturer's instructions.

    Generated:

    Article Title: Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China
    Article Snippet: Viral DNA was extracted from 200 μL of serum samples by using a QIAamp DNA Blood Mini kit (Qiagen Inc., Germany). .. The second stage amplification generated 270-bp product with inner primers (sense primer, 5’-GGGTCACCATATTCTTGGGAA-3’; antisense primer, 5’-Cy5-TGAGGGCTCCACCCCA-3’).

    other:

    Article Title: DNA methylation of a NF-κB binding site in the aquaporin 5 promoter impacts on mortality in sepsis
    Article Snippet: The DNA and RNA were each immediately extracted from whole blood using the QIAamp® DNA Blood Mini Kit (Qiagen, Hilden, Germany) or RNeasy Mini Kit (Qiagen, Hilden, Germany), respectively, according to the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen). .. Resistant parasite populations were assayed by Southern hybridization and PCR to check for gene disruption at the targeted locus.

    Article Title: The Efficacy and Safety of Tyrosine Kinase Inhibitors for Von Hippel–Lindau Disease: A Retrospective Study of 32 Patients
    Article Snippet: Examination of VHL Mutation Genomic DNA was extracted from peripheral blood of suspected individuals using QIAamp DNA Blood Mini Kit (QIAGEN, Germany) according to instructions. .. Three coding exons and flanking intronic regions were amplified by polymerase chain reaction using primers as described in our previous publication ( , ).

    Article Title: Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman‐ARMS Followed a Strict Control System
    Article Snippet: HBV viral DNA was isolated from 200 μl patient serum samples using the QIAamp DNA blood mini kit (Qiagen Inc., Basel, Switzerland) and eluted into 50 μl buffer according to manufacturer's instructions. .. HBV DNA isolated by commercial kits was amplified by PCR in 50 μl of buffer containing 5 μl 10× LAMP buffer, 20 μM dNTP, 0.3 μM each primer, 2.5 mM Mg2+ , and 5 U of LAMP DNA polymerase (Beijing Cowin Bioscience Co., Ltd.).

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. Using ZiFit targeter software[ , ] and Cas-OFFinder [ ] to detect potential off-target sites, eight sets of primers were designed to amplify the 8-potential off-target sites ( ) from the genomic DNA using the online Primer Quest tool (Integrated DNA Technologies) based on available sequences obtained from the NCBI GenBank database and double checked by in-silico PCR amplification using NCBI primer blast tool.

    Article Title: Vitamin D deficiency in head and neck cancer patients – prevalence, prognostic value and impact on immune function
    Article Snippet: For the detection of HPV-DNA, DNA was extracted from fresh-frozen tumor tissue using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. PCR amplification products were detected with SYBR Green as well as gel electrophoresis.

    Article Title: Persistent Elevations in Circulating INS DNA Among Subjects with Longstanding Type 1 Diabetes
    Article Snippet: .. The assay for serum unmethylated and methylated INS DNA using digital droplet PCR has previously been described., Briefly, DNA was extracted from 50 μL of serum using the QiaAmp DNA Blood Mini kit (Qiagen) with 5 μg of poly-A DNA as carrier. .. Bisulfite conversion was performed using the EZ DNA Methylation-Lightning Kit (Zymo Research).

    Article Title: Molecular genetics of late onset glycogen storage disease II in Italy
    Article Snippet: Genomic DNA was extracted with the QIAamp DNA blood Mini Kit (Qiagen GmbH, Hilden, Germany). .. PCR products were screened by denaturing High Performance Liquid Chromatography (dHPLC, Varian, Palo Alto, CA, USA) and in the presence of heteroduplex, sequenced on the ABI PRISM 3700 DNA Analyzer.

    Binding Assay:

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: The plasmid designed to disrupt PbMSH2-2 included a 1380 bp gene fragment , which lacked sequence encoding the first 127 amino acids of the protein, including a portion of the putative DNA binding domain, and was also missing the C-terminal 269 amino acids, including the ATP binding domain and the mutS signature motif. .. Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen).

    DNA Extraction:

    Article Title: Performance of Whole-Genome Amplified DNA Isolated from Serum and Plasma on High-Density Single Nucleotide Polymorphism Arrays
    Article Snippet: Paragraph title: DNA Isolation from Serum and Plasma ... DNA was extracted from 200 μl of serum or plasma using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany).

    Article Title: Interleukin‐21 receptor gene polymorphism is associated with hepatitis B virus‐related hepatocellular carcinoma in Chinese patients, et al. Interleukin‐21 receptor gene polymorphism is associated with hepatitis B virus‐related hepatocellular carcinoma in Chinese patients
    Article Snippet: Paragraph title: Genomic DNA extraction ... Then, genomic DNA was extracted from 200 μL of blood using the QIAamp® DNA Blood Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

    Article Title: Another example of a KEL1 variant red cell phenotype due to a threonine to serine change a position 193 of Kell gp
    Article Snippet: Paragraph title: DNA isolation ... DNA was isolated from EDTA whole blood samples using spin columns (QIAamp DNA Blood Mini Kit, Qiagen, Valencia, CA) according to manufacturer’s instructions and stored at 2°C to 6°C until use.

    Article Title: Application of a methylation gene panel by quantitative PCR for lung cancers
    Article Snippet: Paragraph title: 3. DNA extraction and methylation methods ... Genomic DNA was extracted from sputum samples by using the QIAamp DNA blood mini kit (QIAgen, Venlo, the Netherlands).

    Article Title: Capturing intra-tumor genetic heterogeneity by de novomutation profiling of circulating cell-free tumor DNA: a proof-of-principle
    Article Snippet: Paragraph title: DNA extraction ... DNA from tumor samples was extracted using the RecoverAll™ Total Nucleic Acid Isolation Kit (Ambion, Austin, TX) for FFPE tissue, and germline DNA was extracted from peripheral blood leukocytes using the QIAamp DNA Blood Mini Kit (Qiagen, Valencia, CA), according to the manufacturers' instructions.

    Nucleic Acid Electrophoresis:

    Article Title: Vitamin D deficiency in head and neck cancer patients – prevalence, prognostic value and impact on immune function
    Article Snippet: For the detection of HPV-DNA, DNA was extracted from fresh-frozen tumor tissue using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. PCR amplification products were detected with SYBR Green as well as gel electrophoresis.

    Methylation:

    Article Title: Persistent Elevations in Circulating INS DNA Among Subjects with Longstanding Type 1 Diabetes
    Article Snippet: .. The assay for serum unmethylated and methylated INS DNA using digital droplet PCR has previously been described., Briefly, DNA was extracted from 50 μL of serum using the QiaAmp DNA Blood Mini kit (Qiagen) with 5 μg of poly-A DNA as carrier. .. Bisulfite conversion was performed using the EZ DNA Methylation-Lightning Kit (Zymo Research).

    Article Title: Application of a methylation gene panel by quantitative PCR for lung cancers
    Article Snippet: Paragraph title: 3. DNA extraction and methylation methods ... Genomic DNA was extracted from sputum samples by using the QIAamp DNA blood mini kit (QIAgen, Venlo, the Netherlands).

    Mutagenesis:

    Article Title: The Efficacy and Safety of Tyrosine Kinase Inhibitors for Von Hippel–Lindau Disease: A Retrospective Study of 32 Patients
    Article Snippet: .. Examination of VHL Mutation Genomic DNA was extracted from peripheral blood of suspected individuals using QIAamp DNA Blood Mini Kit (QIAGEN, Germany) according to instructions. .. Three coding exons and flanking intronic regions were amplified by polymerase chain reaction using primers as described in our previous publication ( , ).

    Article Title: Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman‐ARMS Followed a Strict Control System
    Article Snippet: In this study, we developed Taqman amplification refractory mutation system (Taqman‐ARMS) to real time detect G1896A mutant. .. HBV viral DNA was isolated from 200 μl patient serum samples using the QIAamp DNA blood mini kit (Qiagen Inc., Basel, Switzerland) and eluted into 50 μl buffer according to manufacturer's instructions.

    Article Title: Molecular genetics of late onset glycogen storage disease II in Italy
    Article Snippet: Paragraph title: GAA mutation analysis ... Genomic DNA was extracted with the QIAamp DNA blood Mini Kit (Qiagen GmbH, Hilden, Germany).

    Isolation:

    Article Title: Performance of Whole-Genome Amplified DNA Isolated from Serum and Plasma on High-Density Single Nucleotide Polymorphism Arrays
    Article Snippet: DNA was extracted from 200 μl of serum or plasma using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany). .. DNA was isolated in QIAamp spin columns, then eluted, dried, and reconstituted in a final volume of 100 μl of AE buffer.

    Article Title: Another example of a KEL1 variant red cell phenotype due to a threonine to serine change a position 193 of Kell gp
    Article Snippet: .. DNA was isolated from EDTA whole blood samples using spin columns (QIAamp DNA Blood Mini Kit, Qiagen, Valencia, CA) according to manufacturer’s instructions and stored at 2°C to 6°C until use. .. KEL1 Phenotyping was performed using commercial monoclonal anti-KEL1 reagents (Immucor, Norcross, GA) at direct agglutination according to manufacturers’ instructions.

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: .. Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen). .. Resistant parasite populations were assayed by Southern hybridization and PCR to check for gene disruption at the targeted locus.

    Article Title: Accurate Detection of Hepatitis B Virus G1896A Mutant by Developed Taqman‐ARMS Followed a Strict Control System
    Article Snippet: .. HBV viral DNA was isolated from 200 μl patient serum samples using the QIAamp DNA blood mini kit (Qiagen Inc., Basel, Switzerland) and eluted into 50 μl buffer according to manufacturer's instructions. .. Full‐length HBV genome was cloned according to the method of Parekh et al. , .

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: .. Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. DNA concentration and purity at the absorbance ratio 260/280 and 260/230 were determined on a NanoDrop 2000c Spectrophotometer (Thermo-Fisher Scientific).

    Article Title: Capturing intra-tumor genetic heterogeneity by de novomutation profiling of circulating cell-free tumor DNA: a proof-of-principle
    Article Snippet: .. DNA from tumor samples was extracted using the RecoverAll™ Total Nucleic Acid Isolation Kit (Ambion, Austin, TX) for FFPE tissue, and germline DNA was extracted from peripheral blood leukocytes using the QIAamp DNA Blood Mini Kit (Qiagen, Valencia, CA), according to the manufacturers' instructions. .. Plasma-derived DNA was extracted with the QIAamp Circulating Nucleic Acid Kit (Qiagen), as per the manufacturer's protocol.

    Immunohistochemistry:

    Article Title: Vitamin D deficiency in head and neck cancer patients – prevalence, prognostic value and impact on immune function
    Article Snippet: For the determination of HPV tumor status, we combined HPV-DNA-PCR and immunohistochemical p16-Ki67 dual staining. .. For the detection of HPV-DNA, DNA was extracted from fresh-frozen tumor tissue using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Sequencing:

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: The plasmid designed to disrupt PbMSH2-2 included a 1380 bp gene fragment , which lacked sequence encoding the first 127 amino acids of the protein, including a portion of the putative DNA binding domain, and was also missing the C-terminal 269 amino acids, including the ATP binding domain and the mutS signature motif. .. Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen).

    Article Title: The Efficacy and Safety of Tyrosine Kinase Inhibitors for Von Hippel–Lindau Disease: A Retrospective Study of 32 Patients
    Article Snippet: Examination of VHL Mutation Genomic DNA was extracted from peripheral blood of suspected individuals using QIAamp DNA Blood Mini Kit (QIAGEN, Germany) according to instructions. .. Direct sequencing was performed to detect missense mutations, splicing mutations, and small indels.

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: .. Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. DNA concentration and purity at the absorbance ratio 260/280 and 260/230 were determined on a NanoDrop 2000c Spectrophotometer (Thermo-Fisher Scientific).

    Article Title: Molecular genetics of late onset glycogen storage disease II in Italy
    Article Snippet: Genomic DNA was extracted with the QIAamp DNA blood Mini Kit (Qiagen GmbH, Hilden, Germany). .. Mutations were confirmed by sequencing duplicate PCR products and confirmed by the analysis of parental DNA.

    Lysis:

    Article Title: Performance of Whole-Genome Amplified DNA Isolated from Serum and Plasma on High-Density Single Nucleotide Polymorphism Arrays
    Article Snippet: DNA was extracted from 200 μl of serum or plasma using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany). .. Samples were treated with Qiagen protease, then buffer AL (lysis solution) at 56°C for 10 minutes using a TropiCooler (Boekel Scientific, Feasterville, PA).

    Nested PCR:

    Article Title: Detection of hepatitis B virus genotypes using oligonucleotide chip among hepatitis B virus carriers in Eastern China
    Article Snippet: Viral DNA was extracted from 200 μL of serum samples by using a QIAamp DNA Blood Mini kit (Qiagen Inc., Germany). .. The target DNA for hybridization was amplified by a nested PCR.

    Multiplex Ligation-dependent Probe Amplification:

    Article Title: The Efficacy and Safety of Tyrosine Kinase Inhibitors for Von Hippel–Lindau Disease: A Retrospective Study of 32 Patients
    Article Snippet: Examination of VHL Mutation Genomic DNA was extracted from peripheral blood of suspected individuals using QIAamp DNA Blood Mini Kit (QIAGEN, Germany) according to instructions. .. Large deletions and duplications were detected by multiplex ligation-dependent probe amplification (MLPA, P016-C2 kit, MRC-Holland, Amsterdam, the Netherlands).

    Plasmid Preparation:

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: The gene fragment was inserted into the pDb.Dh.^Db vector upstream of a selectable marker expressing the pyramethamine-resistant human dihydrofolate reductase (hDHFR) enzyme [ ]. .. Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen).

    Software:

    Article Title: Chromosomal Aberrations in Pediatric Patients with Developmental Delay/Intellectual Disability: A Single-Center Clinical Investigation
    Article Snippet: Chromosomal Microarray Analysis Whole genomic DNA was extracted from peripheral blood cells of each patient and his or her parents using QIAamp DNA Blood Mini Kit (Qiagen, Valencia, CA, USA) and subjected to CMA-single nucleotide polymorphism (SNP) array analysis by using the Affymetrix® CytoScan™ 750K Array (Affymetrix, Santa Clara, CA, USA). .. When the fragment size of absence of heterozygosity (AOH) was larger than one-third of the chromosome, analysis software UPD tool_0.2 was used to separate the AOH into uniparental disomy (UPD) or consanguinity by comparison with the parental results.

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. Using ZiFit targeter software[ , ] and Cas-OFFinder [ ] to detect potential off-target sites, eight sets of primers were designed to amplify the 8-potential off-target sites ( ) from the genomic DNA using the online Primer Quest tool (Integrated DNA Technologies) based on available sequences obtained from the NCBI GenBank database and double checked by in-silico PCR amplification using NCBI primer blast tool.

    Real-time Polymerase Chain Reaction:

    Article Title: Application of a methylation gene panel by quantitative PCR for lung cancers
    Article Snippet: The modified DNA was used as a template for qPCR analysis. .. Genomic DNA was extracted from sputum samples by using the QIAamp DNA blood mini kit (QIAgen, Venlo, the Netherlands).

    Multiplex Assay:

    Article Title: The Efficacy and Safety of Tyrosine Kinase Inhibitors for Von Hippel–Lindau Disease: A Retrospective Study of 32 Patients
    Article Snippet: Examination of VHL Mutation Genomic DNA was extracted from peripheral blood of suspected individuals using QIAamp DNA Blood Mini Kit (QIAGEN, Germany) according to instructions. .. Large deletions and duplications were detected by multiplex ligation-dependent probe amplification (MLPA, P016-C2 kit, MRC-Holland, Amsterdam, the Netherlands).

    Article Title: Persistent Elevations in Circulating INS DNA Among Subjects with Longstanding Type 1 Diabetes
    Article Snippet: The assay for serum unmethylated and methylated INS DNA using digital droplet PCR has previously been described., Briefly, DNA was extracted from 50 μL of serum using the QiaAmp DNA Blood Mini kit (Qiagen) with 5 μg of poly-A DNA as carrier. .. Samples were analyzed by ddPCR using a dual fluorescent probe-based multiplex assay.

    Spectrophotometry:

    Article Title: Performance of Whole-Genome Amplified DNA Isolated from Serum and Plasma on High-Density Single Nucleotide Polymorphism Arrays
    Article Snippet: DNA was extracted from 200 μl of serum or plasma using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany). .. Initial DNA concentrations and purity were determined using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE).

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. DNA concentration and purity at the absorbance ratio 260/280 and 260/230 were determined on a NanoDrop 2000c Spectrophotometer (Thermo-Fisher Scientific).

    Produced:

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: .. Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. DNA concentration and purity at the absorbance ratio 260/280 and 260/230 were determined on a NanoDrop 2000c Spectrophotometer (Thermo-Fisher Scientific).

    Concentration Assay:

    Article Title: Optimizing sgRNA length to improve target specificity and efficiency for the GGTA1 gene using the CRISPR/Cas9 gene editing system
    Article Snippet: Sanger sequencing of potential off-target sites Genomic DNA was isolated from the WT porcine fetal fibroblasts (PFF), GTKO transfected PFF, GTKO piglet tail fibroblasts (produced and procured under the University of Minnesota IACUC 1711-35291A) using the QIAamp DNA Blood Mini Kit (QIAGEN, Cat. No. 51304), according to manufacturer’s instructions. .. DNA concentration and purity at the absorbance ratio 260/280 and 260/230 were determined on a NanoDrop 2000c Spectrophotometer (Thermo-Fisher Scientific).

    Marker:

    Article Title: The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites
    Article Snippet: The gene fragment was inserted into the pDb.Dh.^Db vector upstream of a selectable marker expressing the pyramethamine-resistant human dihydrofolate reductase (hDHFR) enzyme [ ]. .. Total genomic DNA (gDNA) was isolated from parasite pellets using the QIAamp DNA blood mini kit (Qiagen).

    Staining:

    Article Title: Vitamin D deficiency in head and neck cancer patients – prevalence, prognostic value and impact on immune function
    Article Snippet: HPV-DNA-PCR was performed using fresh-frozen tumor tissue, whereas the p16-Ki67 dual staining was performed on FFPE tumor tissue samples. .. For the detection of HPV-DNA, DNA was extracted from fresh-frozen tumor tissue using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

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  • 90
    Qiagen qiaamp dna blood mini kit
    Qiaamp Dna Blood Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp dna blood mini kit/product/Qiagen
    Average 90 stars, based on 1268 article reviews
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    qiaamp dna blood mini kit - by Bioz Stars, 2020-01
    90/100 stars
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