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plasmid  (Addgene inc)


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    Structured Review

    Addgene inc plasmid
    Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/51026/U6-BbsI-crRNA+(Plasmid+%2351026)/pmc12256344-32-4-2
    Average 93 stars, based on 1 article reviews
    plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Spatiotemporal dynamics of NF-κB/Dorsal inhibitor IκBα/Cactus in Drosophila blastoderm embryos
    Article Snippet: pHD-DsRed , AddGene , Plasmid #51434. .. U6-BbsI-crRNA , AddGene , Plasmid #51026. ..

    Construct:

    Article Title: Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs
    Article Snippet: The PCR product was inserted 10 into the HindIII site of the pPV-EF1α-2xNLS-Cas11-Cas6-Cas3-A vector (Addgene ID: 11 134923) by In-Fusion reaction (Clontech). .. 12 13 To construct Cas3-crRNA and Cas9-sgRNA expression vectors, the synthesized 14 crRNA/sgRNA sequences (Hokkaido System Science) were inserted into the two BbsI 15 sites immediately downstream of the U6 promoter (pBSIIKS-U6v2-BbsI-C1, pBSIIKS-16 U6-BbsI-Cas9 or pPV-U6-crRNA-cloning-EF1α-BA (Addgene ID: 204623)). ..

    Expressing:

    Article Title: Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs
    Article Snippet: The PCR product was inserted 10 into the HindIII site of the pPV-EF1α-2xNLS-Cas11-Cas6-Cas3-A vector (Addgene ID: 11 134923) by In-Fusion reaction (Clontech). .. 12 13 To construct Cas3-crRNA and Cas9-sgRNA expression vectors, the synthesized 14 crRNA/sgRNA sequences (Hokkaido System Science) were inserted into the two BbsI 15 sites immediately downstream of the U6 promoter (pBSIIKS-U6v2-BbsI-C1, pBSIIKS-16 U6-BbsI-Cas9 or pPV-U6-crRNA-cloning-EF1α-BA (Addgene ID: 204623)). ..

    Synthesized:

    Article Title: Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs
    Article Snippet: The PCR product was inserted 10 into the HindIII site of the pPV-EF1α-2xNLS-Cas11-Cas6-Cas3-A vector (Addgene ID: 11 134923) by In-Fusion reaction (Clontech). .. 12 13 To construct Cas3-crRNA and Cas9-sgRNA expression vectors, the synthesized 14 crRNA/sgRNA sequences (Hokkaido System Science) were inserted into the two BbsI 15 sites immediately downstream of the U6 promoter (pBSIIKS-U6v2-BbsI-C1, pBSIIKS-16 U6-BbsI-Cas9 or pPV-U6-crRNA-cloning-EF1α-BA (Addgene ID: 204623)). ..



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    Image Search Results


    Biochemical and biophysical peptide inhibitor potency values for WHSC1 941–1240 and WHSC1L1 1054–1285 <xref ref-type= a ." width="100%" height="100%">

    Journal: PLoS ONE

    Article Title: Identification of a peptide inhibitor for the histone methyltransferase WHSC1

    doi: 10.1371/journal.pone.0197082

    Figure Lengend Snippet: Biochemical and biophysical peptide inhibitor potency values for WHSC1 941–1240 and WHSC1L1 1054–1285 a .

    Article Snippet: GST-tagged WHSC1 941–1240 (#51026) and Avi-tagged WHSC1 941–1240 (#111207) (Genbank #: NM_133330 were purchased from BPS Bioscience.

    Techniques: Sequencing

    Representative peptide inhibitor biochemical dose-response curves for (A) WHSC1 941–1240 and (B) WHSC1L1 1054–1285. Error bars represent the standard deviation of three independent replicates. Resulting IC 50 values are reported in .

    Journal: PLoS ONE

    Article Title: Identification of a peptide inhibitor for the histone methyltransferase WHSC1

    doi: 10.1371/journal.pone.0197082

    Figure Lengend Snippet: Representative peptide inhibitor biochemical dose-response curves for (A) WHSC1 941–1240 and (B) WHSC1L1 1054–1285. Error bars represent the standard deviation of three independent replicates. Resulting IC 50 values are reported in .

    Article Snippet: GST-tagged WHSC1 941–1240 (#51026) and Avi-tagged WHSC1 941–1240 (#111207) (Genbank #: NM_133330 were purchased from BPS Bioscience.

    Techniques: Standard Deviation

    Biochemical IC 50 values for norleucine peptide inhibitors derived from the H4K44 and H3K36 sequences <xref ref-type= a ." width="100%" height="100%">

    Journal: PLoS ONE

    Article Title: Identification of a peptide inhibitor for the histone methyltransferase WHSC1

    doi: 10.1371/journal.pone.0197082

    Figure Lengend Snippet: Biochemical IC 50 values for norleucine peptide inhibitors derived from the H4K44 and H3K36 sequences a .

    Article Snippet: GST-tagged WHSC1 941–1240 (#51026) and Avi-tagged WHSC1 941–1240 (#111207) (Genbank #: NM_133330 were purchased from BPS Bioscience.

    Techniques: Derivative Assay, Sequencing

    PTD2 biochemical IC 50 values for a panel of HMT enzymes.

    Journal: PLoS ONE

    Article Title: Identification of a peptide inhibitor for the histone methyltransferase WHSC1

    doi: 10.1371/journal.pone.0197082

    Figure Lengend Snippet: PTD2 biochemical IC 50 values for a panel of HMT enzymes.

    Article Snippet: GST-tagged WHSC1 941–1240 (#51026) and Avi-tagged WHSC1 941–1240 (#111207) (Genbank #: NM_133330 were purchased from BPS Bioscience.

    Techniques:

    WHSC1 was immobilized on a streptavidin-coated chip and peptide inhibitor was co-injected with SAM utilizing a 3-fold, 5-point dilution series ending at a 20 μM top concentration. Data reported in is presented as the standard deviation of three independent experiments.

    Journal: PLoS ONE

    Article Title: Identification of a peptide inhibitor for the histone methyltransferase WHSC1

    doi: 10.1371/journal.pone.0197082

    Figure Lengend Snippet: WHSC1 was immobilized on a streptavidin-coated chip and peptide inhibitor was co-injected with SAM utilizing a 3-fold, 5-point dilution series ending at a 20 μM top concentration. Data reported in is presented as the standard deviation of three independent experiments.

    Article Snippet: GST-tagged WHSC1 941–1240 (#51026) and Avi-tagged WHSC1 941–1240 (#111207) (Genbank #: NM_133330 were purchased from BPS Bioscience.

    Techniques: Injection, Concentration Assay, Standard Deviation

    Upper panel, calorimetric trace for ligand titration; lower panel, binding isotherm from calorimetric trace. WHSC1 concentration in the cell was 10 μM supplemented with 100 μM SAM. PTD2 concentration in the syringe was 100 μM.

    Journal: PLoS ONE

    Article Title: Identification of a peptide inhibitor for the histone methyltransferase WHSC1

    doi: 10.1371/journal.pone.0197082

    Figure Lengend Snippet: Upper panel, calorimetric trace for ligand titration; lower panel, binding isotherm from calorimetric trace. WHSC1 concentration in the cell was 10 μM supplemented with 100 μM SAM. PTD2 concentration in the syringe was 100 μM.

    Article Snippet: GST-tagged WHSC1 941–1240 (#51026) and Avi-tagged WHSC1 941–1240 (#111207) (Genbank #: NM_133330 were purchased from BPS Bioscience.

    Techniques: Titration, Binding Assay, Concentration Assay

    WHSC1 was immobilized on a streptavidin-coated chip and peptide inhibitor was either injected in the absence of cofactor (left panel), co-injected with SAH (middle panel), or co-injected with SFG (right panel) utilizing a 2-fold, 10-point dilution series ending at a 100 μM top concentration.

    Journal: PLoS ONE

    Article Title: Identification of a peptide inhibitor for the histone methyltransferase WHSC1

    doi: 10.1371/journal.pone.0197082

    Figure Lengend Snippet: WHSC1 was immobilized on a streptavidin-coated chip and peptide inhibitor was either injected in the absence of cofactor (left panel), co-injected with SAH (middle panel), or co-injected with SFG (right panel) utilizing a 2-fold, 10-point dilution series ending at a 100 μM top concentration.

    Article Snippet: GST-tagged WHSC1 941–1240 (#51026) and Avi-tagged WHSC1 941–1240 (#111207) (Genbank #: NM_133330 were purchased from BPS Bioscience.

    Techniques: Injection, Concentration Assay

    (A) Superposition of NSD family proteins (green/yellow = WHSC1L1-PTD2 (PDB code = 6CEN); cyan = WHSC1L1 (PDB code = 5UPD); magenta = WHSC1 (PDB code = 5LSU); purple = NSD1 (PDB code = 3OOI). All protein chains are shown as ribbons; SAM and PTD2 are depicted in stick representation. (B) Structure of WHSC1L1-PTD2-SAM ternary complex. Hydrogen bonds are indicated with dashed lines. (C) Superposition of WHSC1L1-PTD2 and SETD2-H3.3 K36M (grey; PDB code = 5JJY).

    Journal: PLoS ONE

    Article Title: Identification of a peptide inhibitor for the histone methyltransferase WHSC1

    doi: 10.1371/journal.pone.0197082

    Figure Lengend Snippet: (A) Superposition of NSD family proteins (green/yellow = WHSC1L1-PTD2 (PDB code = 6CEN); cyan = WHSC1L1 (PDB code = 5UPD); magenta = WHSC1 (PDB code = 5LSU); purple = NSD1 (PDB code = 3OOI). All protein chains are shown as ribbons; SAM and PTD2 are depicted in stick representation. (B) Structure of WHSC1L1-PTD2-SAM ternary complex. Hydrogen bonds are indicated with dashed lines. (C) Superposition of WHSC1L1-PTD2 and SETD2-H3.3 K36M (grey; PDB code = 5JJY).

    Article Snippet: GST-tagged WHSC1 941–1240 (#51026) and Avi-tagged WHSC1 941–1240 (#111207) (Genbank #: NM_133330 were purchased from BPS Bioscience.

    Techniques:

    Fig. 2. Myxospores of Angiococcus disciformis DSM 52716T (a), Archangium gephyra DSM 2261T (b), Cystobacter minus DSM 14751 (c), Cystobacter violaceus DSM 14727T (d) and Cystobacter fuscus DSM 52657 (e). Bars, 10 mm (a–d), 5 mm (e).

    Journal: International journal of systematic and evolutionary microbiology

    Article Title: Reclassification of Angiococcus disciformis, Cystobacter minus and Cystobacter violaceus as Archangium disciforme comb. nov., Archangium minus comb. nov. and Archangium violaceum comb. nov., unification of the families Archangiaceae and Cystobacteraceae, and emended descriptions of the families Myxococcaceae and Archangiaceae.

    doi: 10.1099/ijsem.0.000533

    Figure Lengend Snippet: Fig. 2. Myxospores of Angiococcus disciformis DSM 52716T (a), Archangium gephyra DSM 2261T (b), Cystobacter minus DSM 14751 (c), Cystobacter violaceus DSM 14727T (d) and Cystobacter fuscus DSM 52657 (e). Bars, 10 mm (a–d), 5 mm (e).

    Article Snippet: Reclassification of Angiococcus disciformis, Cystobacter minus and Cystobacter violaceus as Archangium disciforme comb. nov., Archangium minus comb. nov. and Archangium violaceum comb. nov., unification of the families Archangiaceae and Cystobacteraceae, and emended descriptions of the families Myxococcaceae and Archangiaceae Elke Lang,1 Peter Schumann,1 Brian J. Tindall,1 Kathrin I. Mohr2 and Cathrin Spröer1 Correspondence Elke Lang ela@dsmz.de 1Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124 Braunschweig, Germany 2Helmholtz Centre for Infection Research, Microbial Drugs, Inhoffenstrasse 7, 38124 Braunschweig, Germany The species Archangium gephyra, Angiococcus disciformis, Cystobacter minus and Cystobacter violaceus are currently classified in three different genera of the order Myxococcales.

    Techniques:

    Fig. 3. Fruiting bodies of Angiococcus disciformis DSM 52716T (a), Cystobacter minus DSM 14751 [b, c, d (side view)] and Cystobacter violaceus DSM 14727T (e). Bars, 100 mm (a, c), 200 mm (b, d, e).

    Journal: International journal of systematic and evolutionary microbiology

    Article Title: Reclassification of Angiococcus disciformis, Cystobacter minus and Cystobacter violaceus as Archangium disciforme comb. nov., Archangium minus comb. nov. and Archangium violaceum comb. nov., unification of the families Archangiaceae and Cystobacteraceae, and emended descriptions of the families Myxococcaceae and Archangiaceae.

    doi: 10.1099/ijsem.0.000533

    Figure Lengend Snippet: Fig. 3. Fruiting bodies of Angiococcus disciformis DSM 52716T (a), Cystobacter minus DSM 14751 [b, c, d (side view)] and Cystobacter violaceus DSM 14727T (e). Bars, 100 mm (a, c), 200 mm (b, d, e).

    Article Snippet: Reclassification of Angiococcus disciformis, Cystobacter minus and Cystobacter violaceus as Archangium disciforme comb. nov., Archangium minus comb. nov. and Archangium violaceum comb. nov., unification of the families Archangiaceae and Cystobacteraceae, and emended descriptions of the families Myxococcaceae and Archangiaceae Elke Lang,1 Peter Schumann,1 Brian J. Tindall,1 Kathrin I. Mohr2 and Cathrin Spröer1 Correspondence Elke Lang ela@dsmz.de 1Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124 Braunschweig, Germany 2Helmholtz Centre for Infection Research, Microbial Drugs, Inhoffenstrasse 7, 38124 Braunschweig, Germany The species Archangium gephyra, Angiococcus disciformis, Cystobacter minus and Cystobacter violaceus are currently classified in three different genera of the order Myxococcales.

    Techniques: