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c raciborskii fachb 1503 meryl5 20 bacillus velezensis cr 502  (ATCC)
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ATCC c raciborskii fachb 1503 meryl5 20 bacillus velezensis cr 502
C Raciborskii Fachb 1503 Meryl5 20 Bacillus Velezensis Cr 502, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibody for myhc slow novus nbp2-50299  (Novus Biologicals)
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(A) Representative, triple-labeled images of fiber typing based on immunoreactivity to primary antibodies for <t>MyHC</t> slow (red) and <t>MyHC</t> <t>2A</t> (green) isoforms with the inclusion of a primary antibody for laminin (blue) to clearly define the muscle fiber borders. (B) Scatterplot showing that the relative contribution of interstitial space to total DIAm cross-sectional area was unchanged between 6-month (purple) and 24-month (green) Fischer 344 rats (Student’s unpaired t -test, P > 0.05). (C) Scatterplot showing an increased proportion of type I fibers (circles) and a decreased proportion of type IIx/IIb fibers (triangles) in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (D) Scatterplot showing the mean fiber cross-sectional areas of type I (circles), type IIa (squares), and type IIx/IIb (triangles) fibers. Note the selective reduction in cross-sectional area of type IIx/IIb DIAm fibers in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (E) Scatterplot showing the relative contributions of each fiber type to total DIAm volume. Note the increased contribution of both type I and IIa DIAm fibers and the reduction in type IIx/IIb fibers to total DIAm volume in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). All data sets include female and male rats per age group (see ) with 15 fibers per type in each DIAm.
Primary Antibody For Myhc Slow Novus Nbp2 50299, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mt072917 gs5 bacillus velezensis cr  (ATCC)
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(A) Representative, triple-labeled images of fiber typing based on immunoreactivity to primary antibodies for <t>MyHC</t> slow (red) and <t>MyHC</t> <t>2A</t> (green) isoforms with the inclusion of a primary antibody for laminin (blue) to clearly define the muscle fiber borders. (B) Scatterplot showing that the relative contribution of interstitial space to total DIAm cross-sectional area was unchanged between 6-month (purple) and 24-month (green) Fischer 344 rats (Student’s unpaired t -test, P > 0.05). (C) Scatterplot showing an increased proportion of type I fibers (circles) and a decreased proportion of type IIx/IIb fibers (triangles) in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (D) Scatterplot showing the mean fiber cross-sectional areas of type I (circles), type IIa (squares), and type IIx/IIb (triangles) fibers. Note the selective reduction in cross-sectional area of type IIx/IIb DIAm fibers in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (E) Scatterplot showing the relative contributions of each fiber type to total DIAm volume. Note the increased contribution of both type I and IIa DIAm fibers and the reduction in type IIx/IIb fibers to total DIAm volume in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). All data sets include female and male rats per age group (see ) with 15 fibers per type in each DIAm.
Mt072917 Gs5 Bacillus Velezensis Cr, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mt072908 gl2 bacillus velezensis cr  (ATCC)
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(A) Representative, triple-labeled images of fiber typing based on immunoreactivity to primary antibodies for <t>MyHC</t> slow (red) and <t>MyHC</t> <t>2A</t> (green) isoforms with the inclusion of a primary antibody for laminin (blue) to clearly define the muscle fiber borders. (B) Scatterplot showing that the relative contribution of interstitial space to total DIAm cross-sectional area was unchanged between 6-month (purple) and 24-month (green) Fischer 344 rats (Student’s unpaired t -test, P > 0.05). (C) Scatterplot showing an increased proportion of type I fibers (circles) and a decreased proportion of type IIx/IIb fibers (triangles) in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (D) Scatterplot showing the mean fiber cross-sectional areas of type I (circles), type IIa (squares), and type IIx/IIb (triangles) fibers. Note the selective reduction in cross-sectional area of type IIx/IIb DIAm fibers in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (E) Scatterplot showing the relative contributions of each fiber type to total DIAm volume. Note the increased contribution of both type I and IIa DIAm fibers and the reduction in type IIx/IIb fibers to total DIAm volume in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). All data sets include female and male rats per age group (see ) with 15 fibers per type in each DIAm.
Mt072908 Gl2 Bacillus Velezensis Cr, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mt072911 gl5 bacillus velezensis cr  (ATCC)
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ATCC mt072911 gl5 bacillus velezensis cr
(A) Representative, triple-labeled images of fiber typing based on immunoreactivity to primary antibodies for <t>MyHC</t> slow (red) and <t>MyHC</t> <t>2A</t> (green) isoforms with the inclusion of a primary antibody for laminin (blue) to clearly define the muscle fiber borders. (B) Scatterplot showing that the relative contribution of interstitial space to total DIAm cross-sectional area was unchanged between 6-month (purple) and 24-month (green) Fischer 344 rats (Student’s unpaired t -test, P > 0.05). (C) Scatterplot showing an increased proportion of type I fibers (circles) and a decreased proportion of type IIx/IIb fibers (triangles) in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (D) Scatterplot showing the mean fiber cross-sectional areas of type I (circles), type IIa (squares), and type IIx/IIb (triangles) fibers. Note the selective reduction in cross-sectional area of type IIx/IIb DIAm fibers in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (E) Scatterplot showing the relative contributions of each fiber type to total DIAm volume. Note the increased contribution of both type I and IIa DIAm fibers and the reduction in type IIx/IIb fibers to total DIAm volume in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). All data sets include female and male rats per age group (see ) with 15 fibers per type in each DIAm.
Mt072911 Gl5 Bacillus Velezensis Cr, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egcg (cat no. 50299)  (Millipore)
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(A) Representative, triple-labeled images of fiber typing based on immunoreactivity to primary antibodies for <t>MyHC</t> slow (red) and <t>MyHC</t> <t>2A</t> (green) isoforms with the inclusion of a primary antibody for laminin (blue) to clearly define the muscle fiber borders. (B) Scatterplot showing that the relative contribution of interstitial space to total DIAm cross-sectional area was unchanged between 6-month (purple) and 24-month (green) Fischer 344 rats (Student’s unpaired t -test, P > 0.05). (C) Scatterplot showing an increased proportion of type I fibers (circles) and a decreased proportion of type IIx/IIb fibers (triangles) in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (D) Scatterplot showing the mean fiber cross-sectional areas of type I (circles), type IIa (squares), and type IIx/IIb (triangles) fibers. Note the selective reduction in cross-sectional area of type IIx/IIb DIAm fibers in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (E) Scatterplot showing the relative contributions of each fiber type to total DIAm volume. Note the increased contribution of both type I and IIa DIAm fibers and the reduction in type IIx/IIb fibers to total DIAm volume in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). All data sets include female and male rats per age group (see ) with 15 fibers per type in each DIAm.
Egcg (Cat No. 50299), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Representative, triple-labeled images of fiber typing based on immunoreactivity to primary antibodies for MyHC slow (red) and MyHC 2A (green) isoforms with the inclusion of a primary antibody for laminin (blue) to clearly define the muscle fiber borders. (B) Scatterplot showing that the relative contribution of interstitial space to total DIAm cross-sectional area was unchanged between 6-month (purple) and 24-month (green) Fischer 344 rats (Student’s unpaired t -test, P > 0.05). (C) Scatterplot showing an increased proportion of type I fibers (circles) and a decreased proportion of type IIx/IIb fibers (triangles) in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (D) Scatterplot showing the mean fiber cross-sectional areas of type I (circles), type IIa (squares), and type IIx/IIb (triangles) fibers. Note the selective reduction in cross-sectional area of type IIx/IIb DIAm fibers in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (E) Scatterplot showing the relative contributions of each fiber type to total DIAm volume. Note the increased contribution of both type I and IIa DIAm fibers and the reduction in type IIx/IIb fibers to total DIAm volume in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). All data sets include female and male rats per age group (see ) with 15 fibers per type in each DIAm.

Journal: Frontiers in Physiology

Article Title: Mitochondrial Fragmentation and Dysfunction in Type IIx/IIb Diaphragm Muscle Fibers in 24-Month Old Fischer 344 Rats

doi: 10.3389/fphys.2021.727585

Figure Lengend Snippet: (A) Representative, triple-labeled images of fiber typing based on immunoreactivity to primary antibodies for MyHC slow (red) and MyHC 2A (green) isoforms with the inclusion of a primary antibody for laminin (blue) to clearly define the muscle fiber borders. (B) Scatterplot showing that the relative contribution of interstitial space to total DIAm cross-sectional area was unchanged between 6-month (purple) and 24-month (green) Fischer 344 rats (Student’s unpaired t -test, P > 0.05). (C) Scatterplot showing an increased proportion of type I fibers (circles) and a decreased proportion of type IIx/IIb fibers (triangles) in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (D) Scatterplot showing the mean fiber cross-sectional areas of type I (circles), type IIa (squares), and type IIx/IIb (triangles) fibers. Note the selective reduction in cross-sectional area of type IIx/IIb DIAm fibers in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). (E) Scatterplot showing the relative contributions of each fiber type to total DIAm volume. Note the increased contribution of both type I and IIa DIAm fibers and the reduction in type IIx/IIb fibers to total DIAm volume in 24-month compared with 6-month rats (two-way ANOVA, * P < 0.05). All data sets include female and male rats per age group (see ) with 15 fibers per type in each DIAm.

Article Snippet: One section was double-reacted with a primary antibody for MyHC slow (Novus NBP2-50299) and MyHC 2A (Novus N1511) isoforms.

Techniques: Labeling