Journal: Theranostics

Article Title: Targeted opening of the blood-brain barrier using VCAM-1 functionalised microbubbles and “whole brain” ultrasound

doi: 10.7150/thno.93172

Figure Lengend Snippet: Timeline (A) and Schematic of the experimental set up (B) used for in vivo ultrasound exposure of a mouse model of breast cancer brain metastasis. VCAM-1 targeted microbubbles were destroyed by ultrasound to the heart, immediately upon injection for the control group, whereas in the experimental group, ultrasound destruction at the heart was performed 5 min later to destroy any circulating VCAM-1 microbubbles not bound to the target (panel A was created with Biorender®).

Article Snippet: The polyhistidine-tagged Mouse VCAM-1 protein (50163-M08H) is purchased from Sino Biological (Beijing, China).

Techniques: In Vivo, Injection, Control

Journal: Theranostics

Article Title: Targeted opening of the blood-brain barrier using VCAM-1 functionalised microbubbles and “whole brain” ultrasound

doi: 10.7150/thno.93172

Figure Lengend Snippet: Quantification of VCAM-1 expression at day 14. A) VCAM-1 (brown) immunohistochemistry results of mouse brain sections at day 14 post tumour cell implantation. B) Activated VCAM-1 was detected on the vessels of the left (tumour) hemisphere (red arrows). C) Minimal VCAM-1 expression was found on the vessels of the right (control) side. D) Quantification of VCAM-1 expression, left versus right hemispheres, in animals that were in study at day 14. Sections counterstained with cresyl violet; scale bars = 2 mm (A) and 100 µm (B,C).

Article Snippet: The polyhistidine-tagged Mouse VCAM-1 protein (50163-M08H) is purchased from Sino Biological (Beijing, China).

Techniques: Expressing, Immunohistochemistry, Control