Journal: eLife

Article Title: Plasticity of gene expression in the nervous system by exposure to environmental odorants that inhibit HDACs

doi: 10.7554/eLife.86823

Figure Lengend Snippet: ( A ) Antennal and whole-mount brain staining of Gr63a-Gal4 flies. Flies were exposed to diacetyl (headspace from 10 –2 soln) or air for 2–5 d. Brains and antenna were dissected on the indicated days, fixed, and then stained for neuropil marker nc82 (red) and anti-GFP (green). Magnification 25 x. ( B ) Mean of ab1C neurons expressing GFP after indicated days of odor exposure. d4on = 2,3-butanedione. n = 6, error bars = SEM. Schematic chemical structures of diacetyl, β-hydroxybutyrate, and sodium butyrate. ( C ) Dose–activity curves of class I HDACs: HDAC1, HDAC2, HDAC3, HDAC8, and class II HDAC6 treated with various concentrations of diacetyl. Percentage of HDAC activity is relative to the activity of each enzyme without diacetyl. IC50s are indicated in the chart areas. Error bars = SEM, n = 4–5. ( D ) Representative structures of odorants that inhibit HDACs (left), and average percentage inhibition of class I HDACs: HDAC1, HDAC3, and class II HDAC4, HDAC6 treated with 15 mM of indicated volatiles (right). Error bar = SD, each tested in duplicate.

Article Snippet: Commercial assay or kit , HDAC4 Fluorogenic Assay Kit , BPS Bioscience , 50064 , .

Techniques: Staining, Marker, Expressing, Activity Assay, Inhibition

Journal: eLife

Article Title: Plasticity of gene expression in the nervous system by exposure to environmental odorants that inhibit HDACs

doi: 10.7554/eLife.86823

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , HDAC4 Fluorogenic Assay Kit , BPS Bioscience , 50064 , .

Techniques: Sequencing, Activity Assay, Isolation, SYBR Green Assay, Protease Inhibitor, Western Blot