Journal: Journal of neuroinflammation

Article Title: Hypothalamic kisspeptin alleviates myasthenia gravis by regulating Th1/Th17/Treg balance through Inhibition of NF-κB signaling pathway.

doi: 10.1186/s12974-025-03486-4

Figure Lengend Snippet: Fig. 6 Altered hypothalamic Kiss1 and cFos expression in EAMG rats. (a) Representative immunofluorescence images of Kiss1 and cFos expression in the hypothalamic ARC and AVPV from CFA and EAMG groups. (b-d) Representative immunoblot images (b) and quantitative analysis (c-d) of hypothalamic Kiss1 and cFos levels in CFA and EAMG groups (n = 6). P-values were determined by unpaired t-test. Significance levels: ***p < 0.001, ****p < 0.0001

Article Snippet: Sections were blocked with PBS solution containing 10% goat serum for 1 h. Primary antibodies were incubated overnight at 4 °C: mouse anti-rat C5b-9 antibody (1:100, Santa Cruz, Heidelberg, Germany), Kiss1 (1:50, Invitrogen), or cFos (1:500, Novus, USA).

Techniques: Expressing, Immunofluorescence, Western Blot