covaris m220 focused ultrasonicatortm  (Covaris)


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    Name:
    M220 Focused ultrasonicator
    Description:
    designed for Next Gen Sequencing applications requiring fragment sizes between 150bp and 5kb Compact size and ease of use make the M220 Focused ultrasonicator the ideal DNA shearing solution for MiSeq and PGM users
    Catalog Number:
    500295pgd
    Price:
    None
    Size:
    33cm W 38 7cm D 27 3cm H
    Category:
    Instrument
    Quantity:
    1 0
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    Structured Review

    Covaris covaris m220 focused ultrasonicatortm
    M220 Focused ultrasonicator
    designed for Next Gen Sequencing applications requiring fragment sizes between 150bp and 5kb Compact size and ease of use make the M220 Focused ultrasonicator the ideal DNA shearing solution for MiSeq and PGM users
    https://www.bioz.com/result/covaris m220 focused ultrasonicatortm/product/Covaris
    Average 99 stars, based on 109 article reviews
    Price from $9.99 to $1999.99
    covaris m220 focused ultrasonicatortm - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples"

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples

    Journal: Genes

    doi: 10.3390/genes9010049

    Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].
    Figure Legend Snippet: Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Techniques Used: Next-Generation Sequencing, Sequencing, Amplification

    2) Product Images from "Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples"

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples

    Journal: Genes

    doi: 10.3390/genes9010049

    Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].
    Figure Legend Snippet: Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Techniques Used: Next-Generation Sequencing, Sequencing, Amplification

    Related Articles

    Selection:

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples
    Article Snippet: .. Nuclear DNA Sensitivity and Size Selection Study The single source male control DNA NA24129 (Coriell Institute for Medical Research, Camden, NJ, USA) was mechanically fragmented using the Covaris® M220 Focused-ultrasonicatorTM (Covaris, Woburn, MA, USA) to an average size of 175 bp with a range from 25 bp to 250 bp. .. To test short template sample analysis, size selection was carried out at ≤75 bp using the Pippin Prep® (Sage Science, Beverly, MA, USA).

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples
    Article Snippet: .. DNA Fragmentation and Library Preparation All DNA samples, except the samples in the nuclear DNA sensitivity and size selection study, were mechanically sheared to 250 bp following the manufacturer’s guideline for the Covaris® M220 Focused-ultrasonicatorTM for the 50 µL screw capped tube. .. DNA libraries were then prepared using KAPA Hyper Prep Kit for Illumina® platforms (KAPA Biosystems, Roche® , San Francisco, CA, USA) following the manufacturer’s protocol with optimized PCR cycle numbers ( ).

    Magnetic Beads:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. In preparation for Illumina MiSeq sequencing, 2 μg of gDNA was sheared to an average fragment length of 600 bp using a Covaris M220 ultrasonicator (Woburn, MA), cleaned and concentrated with Agencourt AMPure XP magnetic beads (cat. no. A63880, Beckman Coulter, Indianapolis, IN) as per the manufacturer’s instructions with one minor modification (80% ethanol used for both washes), and eluted in Tris-HCl, pH 8.0. .. DNA quality (260:280 and 260:230) was measured on a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE) and initial average fragment size was verified on the Agilent Tapestation 2200 (Santa Clara, CA) using the D1000 ScreenTape and reagents (cat. no. 5067–5582 and 5067–5583) with an external ladder.

    Next-Generation Sequencing:

    Article Title: Homology-mediated end-capping as a primary step of sister chromatid fusion in the breakage-fusion-bridge cycles
    Article Snippet: .. After fragmentation using the M220 focused ultrasonicator (Covaris), fragments between 100 and 250 bp were purified for NGS library construction using 5500 SOLiD™ Fragment Library Core Kit (Applied Biosystems). ..

    Purification:

    Article Title: Homology-mediated end-capping as a primary step of sister chromatid fusion in the breakage-fusion-bridge cycles
    Article Snippet: .. After fragmentation using the M220 focused ultrasonicator (Covaris), fragments between 100 and 250 bp were purified for NGS library construction using 5500 SOLiD™ Fragment Library Core Kit (Applied Biosystems). ..

    Sequencing:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. In preparation for Illumina MiSeq sequencing, 2 μg of gDNA was sheared to an average fragment length of 600 bp using a Covaris M220 ultrasonicator (Woburn, MA), cleaned and concentrated with Agencourt AMPure XP magnetic beads (cat. no. A63880, Beckman Coulter, Indianapolis, IN) as per the manufacturer’s instructions with one minor modification (80% ethanol used for both washes), and eluted in Tris-HCl, pH 8.0. .. DNA quality (260:280 and 260:230) was measured on a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE) and initial average fragment size was verified on the Agilent Tapestation 2200 (Santa Clara, CA) using the D1000 ScreenTape and reagents (cat. no. 5067–5582 and 5067–5583) with an external ladder.

    Modification:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. In preparation for Illumina MiSeq sequencing, 2 μg of gDNA was sheared to an average fragment length of 600 bp using a Covaris M220 ultrasonicator (Woburn, MA), cleaned and concentrated with Agencourt AMPure XP magnetic beads (cat. no. A63880, Beckman Coulter, Indianapolis, IN) as per the manufacturer’s instructions with one minor modification (80% ethanol used for both washes), and eluted in Tris-HCl, pH 8.0. .. DNA quality (260:280 and 260:230) was measured on a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE) and initial average fragment size was verified on the Agilent Tapestation 2200 (Santa Clara, CA) using the D1000 ScreenTape and reagents (cat. no. 5067–5582 and 5067–5583) with an external ladder.

    Sonication:

    Article Title: BET Bromodomain Inhibition as a Therapeutic Strategy in Ovarian Cancer by Downregulating FoxM1
    Article Snippet: .. Cell pellets were lysed and sonicated using Covaris M220 Ultrasonicator. .. Sonicated lysates were cleared and incubated overnight with magnetic beads bound with IgG, BRD4 (Abcam) or FoxM1 (Cell Signaling Technology) antibodies to enrich for DNA fragments associated with the indicated protein.

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  • 99
    Covaris covaris m220 focused ultrasonicatortm
    Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using <t>Covaris</t> ® <t>M220</t> Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].
    Covaris M220 Focused Ultrasonicatortm, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris m220 focused ultrasonicatortm/product/Covaris
    Average 99 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    covaris m220 focused ultrasonicatortm - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Journal: Genes

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples

    doi: 10.3390/genes9010049

    Figure Lengend Snippet: Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Article Snippet: Nuclear DNA Sensitivity and Size Selection Study The single source male control DNA NA24129 (Coriell Institute for Medical Research, Camden, NJ, USA) was mechanically fragmented using the Covaris® M220 Focused-ultrasonicatorTM (Covaris, Woburn, MA, USA) to an average size of 175 bp with a range from 25 bp to 250 bp.

    Techniques: Next-Generation Sequencing, Sequencing, Amplification