50 bp single end illumina hiseq2000 sequencing  (Illumina Inc)

 
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    Illumina Inc 50 bp single end illumina hiseq2000 sequencing
    50 Bp Single End Illumina Hiseq2000 Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/50 bp single end illumina hiseq2000 sequencing/product/Illumina Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    50 bp single end illumina hiseq2000 sequencing - by Bioz Stars, 2021-07
    86/100 stars

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    Sequencing:

    Article Title: De novo transcriptome assembly databases for the central nervous system of the medicinal leech
    Article Snippet: In order to better balance the single libraries inside the pools, we decide to quantify libraries with qPCR, following the NuGEN suggestion for pool preparation. .. Illumina sequencing The cDNA libraries were generated using messenger RNA-seq (mRNA-seq) assay for transcriptome sequencing on Illumina Hiseq2000 (Data Citation 1 and ). .. Three cDNA libraries were generated from the total RNA of ganglion number 19 and three cDNA libraries were generated from the pooled total RNA of ganglion 10 in equal amounts, and sequencing was performed in one lane to generate 50 bp single end (SE) reads.

    Article Title: Comparative mRNA and miRNA expression in European mouflon (Ovis musimon) and sheep (Ovis aries) provides novel insights into the genetic mechanisms for female reproductive success
    Article Snippet: The RNA concentration and RNA integrity number were measured using a Bioanalyzer 2100 (Agilent Technologies, Waldbronn, Germany). .. Libraries of mRNAs and miRNAs were prepared using the Illumina’s TruSeq library preparation kits and sequenced using Illumina Hiseq2000 at the Finnish Microarray and Sequencing Center, Turku, Finland. .. The mRNA libraries were sequenced using 100 base-pair (bp) paired-end sequencing chemistry, whereas the miRNAs were sequenced using the single-end 50 bp approach.

    Article Title: Complementarity and redundancy of IL-22-producing innate lymphoid cells
    Article Snippet: Quantitative PCR was then performed with the SensiMix SYBR no-Rox kit (Bioline). .. Approximately 5 × 105 lin− (CD3− CD19− ) CD45.2+ cells from each ILC subset were sorted from the intestines of Rorc GFP/+ and Rorc GFP/+ Tbx21+/− mice and two biological replicates were generated and subjected to 50 bp single-end sequencing on an Illumina HiSeq2000 at the Australian Genome Research Facility (Melbourne, Australia). .. More than 30 million reads were generated for each replicate and aligned with the GRCm38/mm10 build of the Mus musculus genome, using the Subread aligner .

    Generated:

    Article Title: De novo transcriptome assembly databases for the central nervous system of the medicinal leech
    Article Snippet: In order to better balance the single libraries inside the pools, we decide to quantify libraries with qPCR, following the NuGEN suggestion for pool preparation. .. Illumina sequencing The cDNA libraries were generated using messenger RNA-seq (mRNA-seq) assay for transcriptome sequencing on Illumina Hiseq2000 (Data Citation 1 and ). .. Three cDNA libraries were generated from the total RNA of ganglion number 19 and three cDNA libraries were generated from the pooled total RNA of ganglion 10 in equal amounts, and sequencing was performed in one lane to generate 50 bp single end (SE) reads.

    Article Title: Complementarity and redundancy of IL-22-producing innate lymphoid cells
    Article Snippet: Quantitative PCR was then performed with the SensiMix SYBR no-Rox kit (Bioline). .. Approximately 5 × 105 lin− (CD3− CD19− ) CD45.2+ cells from each ILC subset were sorted from the intestines of Rorc GFP/+ and Rorc GFP/+ Tbx21+/− mice and two biological replicates were generated and subjected to 50 bp single-end sequencing on an Illumina HiSeq2000 at the Australian Genome Research Facility (Melbourne, Australia). .. More than 30 million reads were generated for each replicate and aligned with the GRCm38/mm10 build of the Mus musculus genome, using the Subread aligner .

    RNA Sequencing Assay:

    Article Title: De novo transcriptome assembly databases for the central nervous system of the medicinal leech
    Article Snippet: In order to better balance the single libraries inside the pools, we decide to quantify libraries with qPCR, following the NuGEN suggestion for pool preparation. .. Illumina sequencing The cDNA libraries were generated using messenger RNA-seq (mRNA-seq) assay for transcriptome sequencing on Illumina Hiseq2000 (Data Citation 1 and ). .. Three cDNA libraries were generated from the total RNA of ganglion number 19 and three cDNA libraries were generated from the pooled total RNA of ganglion 10 in equal amounts, and sequencing was performed in one lane to generate 50 bp single end (SE) reads.

    Immunoprecipitation:

    Article Title: Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants
    Article Snippet: The libraries were sequenced by Fasteris SA (Geneva, Switzerland) on an Illumina HiSeq2000 in a 50 bp single-end run. .. The RNA that was extracted from a VcAGO3 immunoprecipitation was cloned as described before [ ] and sequenced on a MiSeq (Illumina) in a 66 bp single-end run. .. β-elimination treatment of small RNAs Total RNA from vegetative somatic cells and vegetative gonidia was mixed with 20 pmol of a random oligo RNA (5′-UUAGUGAGAGUCCAAUUAAUU-3′, Biomers).

    Clone Assay:

    Article Title: Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants
    Article Snippet: The libraries were sequenced by Fasteris SA (Geneva, Switzerland) on an Illumina HiSeq2000 in a 50 bp single-end run. .. The RNA that was extracted from a VcAGO3 immunoprecipitation was cloned as described before [ ] and sequenced on a MiSeq (Illumina) in a 66 bp single-end run. .. β-elimination treatment of small RNAs Total RNA from vegetative somatic cells and vegetative gonidia was mixed with 20 pmol of a random oligo RNA (5′-UUAGUGAGAGUCCAAUUAAUU-3′, Biomers).

    Microarray:

    Article Title: Comparative mRNA and miRNA expression in European mouflon (Ovis musimon) and sheep (Ovis aries) provides novel insights into the genetic mechanisms for female reproductive success
    Article Snippet: The RNA concentration and RNA integrity number were measured using a Bioanalyzer 2100 (Agilent Technologies, Waldbronn, Germany). .. Libraries of mRNAs and miRNAs were prepared using the Illumina’s TruSeq library preparation kits and sequenced using Illumina Hiseq2000 at the Finnish Microarray and Sequencing Center, Turku, Finland. .. The mRNA libraries were sequenced using 100 base-pair (bp) paired-end sequencing chemistry, whereas the miRNAs were sequenced using the single-end 50 bp approach.

    Purification:

    Article Title: N6-methyladenosine-dependent RNA structural switches regulate RNA-protein interactions
    Article Snippet: After that, the RNA was eluted by 1 hour incubation with 20 μl elution buffer (1× IP buffer and 6.7 mM m6 A, Sigma-Aldrich) under gentle shaking conditions, and purified by ethanol precipitation. .. The purified RNA sample (IP) as well as the input PAR-CLIP RNA sample (Input control) were used for library construction by Truseq small RNA sample preparation kit (Illumina). .. Libraries were prepared using TruSeq Small RNA Sample Preparation Kit (RS-200-0012, Illumina) according to the manufacturer's instructions, and then sequenced by Illumina Hiseq2000 with single end 50-bp read length.

    Sample Prep:

    Article Title: N6-methyladenosine-dependent RNA structural switches regulate RNA-protein interactions
    Article Snippet: After that, the RNA was eluted by 1 hour incubation with 20 μl elution buffer (1× IP buffer and 6.7 mM m6 A, Sigma-Aldrich) under gentle shaking conditions, and purified by ethanol precipitation. .. The purified RNA sample (IP) as well as the input PAR-CLIP RNA sample (Input control) were used for library construction by Truseq small RNA sample preparation kit (Illumina). .. Libraries were prepared using TruSeq Small RNA Sample Preparation Kit (RS-200-0012, Illumina) according to the manufacturer's instructions, and then sequenced by Illumina Hiseq2000 with single end 50-bp read length.

    Mouse Assay:

    Article Title: Complementarity and redundancy of IL-22-producing innate lymphoid cells
    Article Snippet: Quantitative PCR was then performed with the SensiMix SYBR no-Rox kit (Bioline). .. Approximately 5 × 105 lin− (CD3− CD19− ) CD45.2+ cells from each ILC subset were sorted from the intestines of Rorc GFP/+ and Rorc GFP/+ Tbx21+/− mice and two biological replicates were generated and subjected to 50 bp single-end sequencing on an Illumina HiSeq2000 at the Australian Genome Research Facility (Melbourne, Australia). .. More than 30 million reads were generated for each replicate and aligned with the GRCm38/mm10 build of the Mus musculus genome, using the Subread aligner .

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    Illumina Inc illumina hiseq2000 instrument
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