5×t4 pnk buffer a  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 5×t4 pnk buffer a
    5×T4 Pnk Buffer A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5×t4 pnk buffer a/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5×t4 pnk buffer a - by Bioz Stars, 2020-04
    86/100 stars

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    rna probe
    t4 pnk

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    Synthesized:

    Article Title: m6A-dependent regulation of messenger RNA stability
    Article Snippet: EMSA (Electrophoretic Mobility Shift Assay / Gel shift assay) The RNA probe was synthesized by a previously reported method with the sequence of 5’-AUGGGCCGUUCAUCUGCUAAAAGGXCUGCUUUUGGGGCUUGU-3’(X = A or m6 A). .. After the synthesis, the RNA probe was labeled in a reaction mixture of 2 µL RNA probe (1 µM), 5 µL 5×T4 PNK buffer A (Fermentas), 1 µL T4 PNK (Fermentas), 1 µL P-ATP and 41 µL RNase-free water (final RNA concentration 40 nM) at 37°C for 1 hour.

    Labeling:

    Article Title: m6A-dependent regulation of messenger RNA stability
    Article Snippet: .. After the synthesis, the RNA probe was labeled in a reaction mixture of 2 µL RNA probe (1 µM), 5 µL 5×T4 PNK buffer A (Fermentas), 1 µL T4 PNK (Fermentas), 1 µL P-ATP and 41 µL RNase-free water (final RNA concentration 40 nM) at 37°C for 1 hour. .. The mixture was then purified by RNase-free micro bio-spin columns with bio-gel P30 in Tris buffer (BioRad 732–6250) to remove hot ATP and other small molecules.

    Purification:

    Article Title: m6A-dependent regulation of messenger RNA stability
    Article Snippet: After the synthesis, the RNA probe was labeled in a reaction mixture of 2 µL RNA probe (1 µM), 5 µL 5×T4 PNK buffer A (Fermentas), 1 µL T4 PNK (Fermentas), 1 µL P-ATP and 41 µL RNase-free water (final RNA concentration 40 nM) at 37°C for 1 hour. .. The mixture was then purified by RNase-free micro bio-spin columns with bio-gel P30 in Tris buffer (BioRad 732–6250) to remove hot ATP and other small molecules.

    Concentration Assay:

    Article Title: m6A-dependent regulation of messenger RNA stability
    Article Snippet: .. After the synthesis, the RNA probe was labeled in a reaction mixture of 2 µL RNA probe (1 µM), 5 µL 5×T4 PNK buffer A (Fermentas), 1 µL T4 PNK (Fermentas), 1 µL P-ATP and 41 µL RNase-free water (final RNA concentration 40 nM) at 37°C for 1 hour. .. The mixture was then purified by RNase-free micro bio-spin columns with bio-gel P30 in Tris buffer (BioRad 732–6250) to remove hot ATP and other small molecules.

    Electrophoretic Mobility Shift Assay:

    Article Title: m6A-dependent regulation of messenger RNA stability
    Article Snippet: Paragraph title: EMSA (Electrophoretic Mobility Shift Assay / Gel shift assay) ... After the synthesis, the RNA probe was labeled in a reaction mixture of 2 µL RNA probe (1 µM), 5 µL 5×T4 PNK buffer A (Fermentas), 1 µL T4 PNK (Fermentas), 1 µL P-ATP and 41 µL RNase-free water (final RNA concentration 40 nM) at 37°C for 1 hour.

    Sequencing:

    Article Title: m6A-dependent regulation of messenger RNA stability
    Article Snippet: EMSA (Electrophoretic Mobility Shift Assay / Gel shift assay) The RNA probe was synthesized by a previously reported method with the sequence of 5’-AUGGGCCGUUCAUCUGCUAAAAGGXCUGCUUUUGGGGCUUGU-3’(X = A or m6 A). .. After the synthesis, the RNA probe was labeled in a reaction mixture of 2 µL RNA probe (1 µM), 5 µL 5×T4 PNK buffer A (Fermentas), 1 µL T4 PNK (Fermentas), 1 µL P-ATP and 41 µL RNase-free water (final RNA concentration 40 nM) at 37°C for 1 hour.

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    Thermo Fisher t4 pnk
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