5 xhoi 3 paci restriction sites  (Thermo Fisher)


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  • 92

    Structured Review

    Thermo Fisher 5 xhoi 3 paci restriction sites
    5 Xhoi 3 Paci Restriction Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 xhoi 3 paci restriction sites/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 xhoi 3 paci restriction sites - by Bioz Stars, 2020-04
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Frequent adaptive immune responses against arginase-1
    Article Snippet: .. The cDNA encoding Arginase-1 (accession nr. ) was synthesized and cloned into either pSP73-SphA64 (kindly provided by Dr. E. Gilboa, Duke University Medical Center, Durham, NC) using 5′XhoI/3′PacI restriction sites (Geneart/Life Technologies) or into the HLA class II targeting plasmid pGEM-sig-DC.LAMP (kindly provided by Dr. K. Thielemans, Medical School of the Vrije Universiteit Brussel) using 5′BamHI/3′BamHI restriction sites. .. Both plasmids were linearized with SpeI before serving as DNA template for in vitro transcription.

    In Vitro:

    Article Title: Frequent adaptive immune responses against arginase-1
    Article Snippet: The cDNA encoding Arginase-1 (accession nr. ) was synthesized and cloned into either pSP73-SphA64 (kindly provided by Dr. E. Gilboa, Duke University Medical Center, Durham, NC) using 5′XhoI/3′PacI restriction sites (Geneart/Life Technologies) or into the HLA class II targeting plasmid pGEM-sig-DC.LAMP (kindly provided by Dr. K. Thielemans, Medical School of the Vrije Universiteit Brussel) using 5′BamHI/3′BamHI restriction sites. .. Both plasmids were linearized with SpeI before serving as DNA template for in vitro transcription.

    Plasmid Preparation:

    Article Title: Frequent adaptive immune responses against arginase-1
    Article Snippet: .. The cDNA encoding Arginase-1 (accession nr. ) was synthesized and cloned into either pSP73-SphA64 (kindly provided by Dr. E. Gilboa, Duke University Medical Center, Durham, NC) using 5′XhoI/3′PacI restriction sites (Geneart/Life Technologies) or into the HLA class II targeting plasmid pGEM-sig-DC.LAMP (kindly provided by Dr. K. Thielemans, Medical School of the Vrije Universiteit Brussel) using 5′BamHI/3′BamHI restriction sites. .. Both plasmids were linearized with SpeI before serving as DNA template for in vitro transcription.

    Synthesized:

    Article Title: Frequent adaptive immune responses against arginase-1
    Article Snippet: .. The cDNA encoding Arginase-1 (accession nr. ) was synthesized and cloned into either pSP73-SphA64 (kindly provided by Dr. E. Gilboa, Duke University Medical Center, Durham, NC) using 5′XhoI/3′PacI restriction sites (Geneart/Life Technologies) or into the HLA class II targeting plasmid pGEM-sig-DC.LAMP (kindly provided by Dr. K. Thielemans, Medical School of the Vrije Universiteit Brussel) using 5′BamHI/3′BamHI restriction sites. .. Both plasmids were linearized with SpeI before serving as DNA template for in vitro transcription.