5 bromouridine 5 triphosphato brutp (Millipore)

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5 Bromouridine

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Millipore 5 bromouridine 5 triphosphato brutp

https://www.bioz.com/result/5 bromouridine 5 triphosphato brutp/product/Millipore
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

5 bromouridine 5 triphosphato brutp - by Bioz Stars, 2021-03

93/100 stars

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Labeling:

Article Title: Inhibition of cdk9 during Herpes Simplex Virus 1 Infection Impedes Viral Transcription
Article Snippet: Cells were stained with anti-ICP4 (P1101) at 1:500; anti-ICP27 (P1119) at 1:500; anti-RNAP II antibody ARNA3 (Research Diagnostics) at 1:50; anti-bromodeoxyuridine Ab-3 (Calbiochem) at 1:100; anti-HA (Sigma) at 1:500 and anti-FLAG (Sigma) at 1:500. .. Bromouridine labeling and in situ hybridization To label nascent, newly synthesized RNA, 4 mM 5-Bromouridine (BrU-Sigma-Aldrich) was added to the culture medium for 30 min at 37°C before fixation of the cells, which were subsequently stained with anti-BrU antibody (Ab3; Calbiochem). .. For poly(A)+ RNA hybridization, cells grown on coverslips in 24 well dishes were infected as described in the figure legends and were fixed with 3.7% formaldehyde and then overlaid with 70% ethanol at 4°C.

Article Title: A transient ischemic environment induces reversible compaction of chromatin
Article Snippet: ATP was determined using a bioluminescence assay utilizing recombinant firefly luciferase and its substrate D-luciferin (ATP Determination Kit, Invitrogen). .. Bromouridine labeling of nascent RNA Newly synthesized RNA was pulse labeled by incubating confluent 10-cm culture dishes with 2 mM bromouridine (BrU; Sigma Aldrich) for 1 hour, either under normal culture conditions, under OND, or after 1 hour of recovery from OND. ..

Article Title: Three Arginine Residues within the RGG Box Are Crucial for ICP27 Binding to Herpes Simplex Virus 1 GC-Rich Sequences and for Efficient Viral RNA Export ▿Three Arginine Residues within the RGG Box Are Crucial for ICP27 Binding to Herpes Simplex Virus 1 GC-Rich Sequences and for Efficient Viral RNA Export ▿ †
Article Snippet: HeLa cells were infected at a multiplicity of infection (MOI) of 10 with HSV-1 KOS or the ΔRGG, R138,150K, and R138,148,150K RGG box mutants. .. At 7 h after infection cells were labeled with 4 mM 5-bromouridine (BrU) (Sigma) for 30 min. .. Cells were washed twice with phosphate-buffered saline (PBS) and then incubated with complete medium without 5-BrU for 45 min before the cells were fixed with 3.7% formaldehyde.

In Situ Hybridization:

Synthesized:

Staining:

Multiple Displacement Amplification:

Article Title: Immunogenicity of mammary tumor cells can be induced by shikonin via direct binding-interference with hnRNPA1
Article Snippet: Fluorescence imaging was performed and captured on a LSM 780 inverted Confocal plus Super Resolution Microscope (Zeiss). .. BrU Incorporation 4T1, 4T1-hnRNPA1, MDA-MD-231 or MDA-MD-231-hnRNPA1 cells were grown overnight on coverslips, washed 3 times with HBSS and placed in 100 μl cell buffer containing 2 mM bromouridine (Sigma). ..

Immuno-Electron Microscopy:

Article Title: Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription
Article Snippet: For immunoelectron microscopy, cells were fixed with 4% paraformaldehyde in 0.1 M Sörensen phosphate buffer at 4°C for 1 h, washed, treated with 0.5M NH4 Cl in PBS, dehydrated with ethanol and embedded in LRWhite resin. .. In order to evaluate RNA transcription rate, some cells were pulse-labelled with 10 mM BrU (5-bromouridine, Sigma-Aldrich) for 10 min at 37°C, and then fixed and processed for immunoelectron microscopy as above. .. Ultrathin sections from Epon-embedded samples were conventionally stained with lead citrate and observed.

RNA Sequencing Assay:

Article Title: Molecular basis of differential 3′ splice site sensitivity to anti-tumor drugs targeting U2 snRNP
Article Snippet: .. BrU-IP and RNA-Seq analysis HeLa cells (800,000 in a 6 cm plate) were co-treated with 2 mM BrU (5-Bromouridine, Sigma-Aldrich) and 10 µM Sud C1, 2 µM Sud K, or 5 nM SSA A for 3 h. RNA was extracted using the Maxwell RNAeasy kit (Promega). .. Total RNA was kept as input and for standard RNA sequencing.

Infection:

Concentration Assay:

Article Title: Gene length as a biological timer to establish temporal transcriptional regulation
Article Snippet: .. Briefly, bromouridine (Bru) (Aldrich) was added to the media of starved or serum stimulated cells to a final concentration of 2 mM and incubated at 37°C for 30 min. BruUV-seq was performed as previously described using a UV dose of 100 J/m2 prior to Bru-labeling. .. Total RNA was isolated using TRIzol reagent (Invitrogen) and Bru-labeled RNA was isolated from total RNA by incubation with anti-BrdU antibodies (BD Biosciences) conjugated to magnetic Dynabeads (Invitrogen) under gentle agitation at room temperature for 1h. cDNA libraries were generated from the isolated Bru-labeled RNA using the Illumina TruSeq kit, modified as described previously to produce strand-specific and size-selected libraries which were then sequenced using the Illumina HiSeq 2500 platform (50 base, single-ended reads).