5 n ethylcarboxamidoadenosine neca (Millipore)

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5 N Ethylcarboxamido adenosine

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Catalog Number:

e2387

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Structured Review

Millipore 5 n ethylcarboxamidoadenosine neca
5 N Ethylcarboxamido adenosine

https://www.bioz.com/result/5 n ethylcarboxamidoadenosine neca/product/Millipore
Average 99 stars, based on 1925 article reviews
Price from $9.99 to $1999.99

5 n ethylcarboxamidoadenosine neca - by Bioz Stars, 2021-01

99/100 stars

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Images

1) Product Images from "Adenosine receptors and second messenger signaling pathways in rat cardiac fibroblasts"

Article Title: Adenosine receptors and second messenger signaling pathways in rat cardiac fibroblasts

Journal: American Journal of Physiology - Cell Physiology

doi: 10.1152/ajpcell.00290.2008

cAMP accumulation in response to adenosine (ADO) agonists. A : CF were preincubated with 10 μM rolipram for 15 min and then treated with 10 μM CADO, 100 μM 5′- N -ethylcarboxamidoadensoine (NECA), or 1 μM isoproterenol

Figure Legend Snippet: cAMP accumulation in response to adenosine (ADO) agonists. A : CF were preincubated with 10 μM rolipram for 15 min and then treated with 10 μM CADO, 100 μM 5′- N -ethylcarboxamidoadensoine (NECA), or 1 μM isoproterenol

Techniques Used:

2) Product Images from "Translocation of neuronal nitric oxide synthase to the plasma membrane by ATP is mediated by P2X and P2Y receptors"

Article Title: Translocation of neuronal nitric oxide synthase to the plasma membrane by ATP is mediated by P2X and P2Y receptors

Journal: Molecular Pain

doi: 10.1186/1744-8069-5-40

Signal pathways coupled to the ATP-induced nNOSNT-YFP translocation . Effect of kinase inhibitors on the translocation of nNOSNT-YFP induced by NMDA, forskolin, and ATP. After a 30-min incubation of NGF-differentiated PC12N cells with vehicle or with 100 μM NMDA, 100 μM ATP, and 10 μM forskolin (FK) in the presence of 0.1 μM calphostin C (Cal C), 1 μM KN-62, 1 μM PP2, 50 nM H-89, 25 μM LY294002 (LY), 25 μM PD98059 (PD), 20 μM SB203580 (SB) or 25 μM roscovitine (RV), the translocation of nNOSNT-YFP was observed by confocal microscopy and quantified as described in the legend for Figure 1B. Data are presented as the mean ± SEM (n = 4). * p

Figure Legend Snippet: Signal pathways coupled to the ATP-induced nNOSNT-YFP translocation . Effect of kinase inhibitors on the translocation of nNOSNT-YFP induced by NMDA, forskolin, and ATP. After a 30-min incubation of NGF-differentiated PC12N cells with vehicle or with 100 μM NMDA, 100 μM ATP, and 10 μM forskolin (FK) in the presence of 0.1 μM calphostin C (Cal C), 1 μM KN-62, 1 μM PP2, 50 nM H-89, 25 μM LY294002 (LY), 25 μM PD98059 (PD), 20 μM SB203580 (SB) or 25 μM roscovitine (RV), the translocation of nNOSNT-YFP was observed by confocal microscopy and quantified as described in the legend for Figure 1B. Data are presented as the mean ± SEM (n = 4). * p

Techniques Used: Translocation Assay, Incubation, Confocal Microscopy

3) Product Images from "Adenosine receptors and second messenger signaling pathways in rat cardiac fibroblasts"

Article Title: Adenosine receptors and second messenger signaling pathways in rat cardiac fibroblasts

Journal: American Journal of Physiology - Cell Physiology

doi: 10.1152/ajpcell.00290.2008

Effects of phosphodiesterase (PDE) inhibitors on cAMP accumulation. CF were pretreated in the absence or presence of 1 mM 3-isobutyl-1-methylxanthine (IBMX) or 10 μM rolipram for 15 min and then stimulated with or without 10 μM 2-chloroadenosine

Figure Legend Snippet: Effects of phosphodiesterase (PDE) inhibitors on cAMP accumulation. CF were pretreated in the absence or presence of 1 mM 3-isobutyl-1-methylxanthine (IBMX) or 10 μM rolipram for 15 min and then stimulated with or without 10 μM 2-chloroadenosine

Techniques Used:

Binding Assay:

Article Title: Docking Screens for Dual Inhibitors of Disparate Drug Targets for Parkinson’s Disease
Article Snippet: .. Nonspecific binding was determined in the presence of 50 μM NECA (Sigma E2387). .. A1 AR competition binding experiments were carried out in a multiscreen GF/C 96-well plate (Millipore, Madrid, Spain) pretreated with binding buffer (Hepes 20 mM, NaCl 100 mM, MgCl2 10 mM, 2 U/mL adenosine deaminase, pH = 7.4).

other:

Article Title: Hypoxia induces T-cell apoptosis by inhibiting chemokine C receptor 7 expression: the role of adenosine receptor A2
Article Snippet: 5′- N -ethyl-carboxamidoadenosine (NECA), a nonspecific agonist for all ARs, N 6 -cyclopentyladenosine (CPA), an agonist specific for A1 receptor, 2-[ p -2-(carboxy-ethyl)-phenylethylamino]-5′- N -ethylcarboxamidoadenosine , an agonist specific for A2a receptor, N 6 -(3-iodobenzyl)adenosine-5'- N -methylluronamide (IB-MECA), an agonist specific for A3 receptor, 5-amino-2-(2-furyl)-7-phenylethyl-pyrazolo-[4,3-e]-1,2,4-triazolo[1,5-c] pyrimidine , an antagonist specific for A2a receptor, phytohaemagglutinin, lipopolysaccharide (LPS), Ficoll and nylon were all obtained from Sigma (St. Louis, MO, USA).

Article Title: Effects of adenine nucleosides and nucleotides on neuromuscular transmission to the prostatic stroma of the rat
Article Snippet: The following drugs were used: adenosine (Sigma), adenosine deaminase (Sigma), adenosine 5′-diphosphate (ADP; Sigma), adenosine 5′-monophosphate (AMP; Sigma), adenosine 5′-triphosphate (ATP; Sigma), 2-p-[2-carboxyethyl]phenethyl-amino-5′-N-ethylcarboxamido-adenosine (CGS 21680; Sigma), N6 -cyclohexyladenosine (CHA; Sigma), N6 -cyclopentyladenosine (CPA; Sigma), 2-methylthio ATP (Sigma), 5′-(N-ethylcarboxamido)-adenosine (NECA; Sigma), noradrenaline, (Sigma), 8-phenyltheophylline (Sigma), (−)-N6 -(2-phenylisopropyl)-adenosine (R-PIA; Sigma), (+)-N6 -(2-phenylisopropyl)-adenosine (S-PIA; Sigma), reactive blue 2 (Tocris) and suramin (Sigma).