Structured Review

ATUM 5 biotinylated 80 mer poly dt oligo dna
5 Biotinylated 80 Mer Poly Dt Oligo Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more biotinylated 80 mer poly dt oligo dna/product/ATUM
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
5 biotinylated 80 mer poly dt oligo dna - by Bioz Stars, 2020-04
90/100 stars


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Article Title: FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5′-DNA end
Article Snippet: .. RAD51 transfer assay Chicken RAD51 (5.2 μM) was incubated with a 5′-biotinylated 80-mer poly dT oligo DNA (20.7 μM), a 5′-biotinylated 3′-tailed DNA (20.7 μM) or a 5′-biotinylated replication fork-like DNA (20.7 μM) conjugated to Dynabeads M-280 Streptavidin for 10 min at 37°C, in 11.6 μl of reaction buffer, containing 60 mM Tris-HCl (pH 8.0), 6% glycerol, 108 mM NaCl, 4.3 mM MgCl2 , 1.7 mM adenosine triphosphate (ATP) and 1.7 mM dithiothreitol. .. After the incubation, the I-D complex (2.7 μM), which was preincubated with or without the 49-mer dsDNA (20 μM) for 10 min at 37°C in 6 μl of 13 mM Tris-HCl (pH 8.0) buffer, containing 6.7% glycerol, 133 mM NaCl and 3.3 mM 2-mercaptoethanol, was added to the reaction mixture, followed by an incubation for 10 min at 37°C.