5 bb0002mutr xbai  (Thermo Fisher)


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  • 99
    Name:
    XbaI
    Description:
    5 T ↓C T A G A 3 3 A G A T C ↑T 5 Thermo Scientific XbaI restriction enzyme recognizes T CTAGA sites and cuts best at 37°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0683
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher 5 bb0002mutr xbai
    5 T ↓C T A G A 3 3 A G A T C ↑T 5 Thermo Scientific XbaI restriction enzyme recognizes T CTAGA sites and cuts best at 37°C in Tango buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/5 bb0002mutr xbai/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 bb0002mutr xbai - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei
    Article Snippet: .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240rev-CAT/EP2 (1240R ): the first 1240 bp of PAG1 were amplified by PCR from pG-1240-CAT/EP2 with the primers PAG1down3 and 1240up3 and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240-neo-CAT/EP2 (1240F -neo): the same strategy was used as for pG-640-neo-CAT/EP2, however the neo fragment was cloned into XbaI site of pG-1240-CAT/EP2. pG-PAG1end-CAT/EP2 (ORFF ): to obtain the fragment with the PAG1 sequence from its internal HindIII site at 1087 to position 2458, a PCR was performed with the template pBS-711-HN ( ) and the primers M13r (Invitrogen) and PAG1up2. .. The product was digested with HindIII and XbaI and cloned into the corresponding sites of pG-mcs-CAT/EP2. pG-PAG1end-rev-CAT/EP2 (ORFR ): the same strategy was used as for pG-PAG1end-CAT/EP2 using the primers PAGmid and PAG1up3. pG-662_1240-CAT/EP2 (MidF ): the PAG1 sequence from 662 to 1240 was amplified by PCR with the primers 640down and 1240up on template pG-1240-CAT/EP2.

    Amplification:

    Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei
    Article Snippet: .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240rev-CAT/EP2 (1240R ): the first 1240 bp of PAG1 were amplified by PCR from pG-1240-CAT/EP2 with the primers PAG1down3 and 1240up3 and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240-neo-CAT/EP2 (1240F -neo): the same strategy was used as for pG-640-neo-CAT/EP2, however the neo fragment was cloned into XbaI site of pG-1240-CAT/EP2. pG-PAG1end-CAT/EP2 (ORFF ): to obtain the fragment with the PAG1 sequence from its internal HindIII site at 1087 to position 2458, a PCR was performed with the template pBS-711-HN ( ) and the primers M13r (Invitrogen) and PAG1up2. .. The product was digested with HindIII and XbaI and cloned into the corresponding sites of pG-mcs-CAT/EP2. pG-PAG1end-rev-CAT/EP2 (ORFR ): the same strategy was used as for pG-PAG1end-CAT/EP2 using the primers PAGmid and PAG1up3. pG-662_1240-CAT/EP2 (MidF ): the PAG1 sequence from 662 to 1240 was amplified by PCR with the primers 640down and 1240up on template pG-1240-CAT/EP2.

    Article Title: Characterization of the PcCdc42 small G protein from Pneumocystis carinii, which interacts with the PcSte20 life cycle regulatory kinase
    Article Snippet: .. In addition, the 576-bp amplicon was also hybridized to Pc genomic DNA digested with the restriction enzymes Bam HI, Xho I, and Xba I (Invitrogen). .. In parallel reactions, rat genomic DNA (Bioline, Randolph, MA) was digested with the same restriction enzymes and hybridized in a similar fashion.

    Agarose Gel Electrophoresis:

    Article Title: The bandit, a New DNA Transposon from a Hookworm--Possible Horizontal Genetic Transfer between Host and Parasite
    Article Snippet: .. Briefly, gDNA was digested with the endonuclease Hin d III and Xba I (Fermentas, Sweden) and size separated through 0.8% agarose gel. .. Fragments ranging in size from 2–7 kilobase pairs (kb) were excised, eluted from the gel, and ligated into plasmid pBluescript SK (+/−) (Stratagene).

    Article Title: A Molecular Epidemiological Study of Mycobacterium simiae Isolated from AIDS Patients in Guadeloupe
    Article Snippet: .. Plugs were prepared as previously described , and bacterial DNA was digested with 30 U of Dra I or 60 U of Xba I (Gibco-BRL Life Technologies) at 37°C for 2 h. After digestion, the plugs were loaded into a 1% (wt/vol) agarose gel (Gibco BRL). .. Large restriction fragments were separated using the contour-clamped homogeneous electric field DRIII pulsed-field gel electrophoresis (PFGE) apparatus (Bio-Rad, Richmond, Calif.) for 24 h at 14°C and 6 V/cm with a switch time of 1 to 40 s for Dra I and for 20 h at 14°C and 6 V/cm with a switch time of 1 to 30 s for Xba I.

    Labeling:

    Article Title: Developmental Specificity of the Interaction between the Locus Control Region and Embryonic or Fetal Globin Genes in Transgenic Mice with an HS3 Core Deletion
    Article Snippet: .. Digestion with Xba I and Xho I released a 1.3-kb fragment that was labeled with a Decaprime II random labeling kit (Ambion). .. As an internal control during Southern blot hybridization, we digested pThy1.1/3′ A γ(753) with Pst I and Sca I to release a 2.6-kb A γ fragment and a 1.6-kb Thy1.1 fragment.

    Polymerase Chain Reaction:

    Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei
    Article Snippet: .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240rev-CAT/EP2 (1240R ): the first 1240 bp of PAG1 were amplified by PCR from pG-1240-CAT/EP2 with the primers PAG1down3 and 1240up3 and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240-neo-CAT/EP2 (1240F -neo): the same strategy was used as for pG-640-neo-CAT/EP2, however the neo fragment was cloned into XbaI site of pG-1240-CAT/EP2. pG-PAG1end-CAT/EP2 (ORFF ): to obtain the fragment with the PAG1 sequence from its internal HindIII site at 1087 to position 2458, a PCR was performed with the template pBS-711-HN ( ) and the primers M13r (Invitrogen) and PAG1up2. .. The product was digested with HindIII and XbaI and cloned into the corresponding sites of pG-mcs-CAT/EP2. pG-PAG1end-rev-CAT/EP2 (ORFR ): the same strategy was used as for pG-PAG1end-CAT/EP2 using the primers PAGmid and PAG1up3. pG-662_1240-CAT/EP2 (MidF ): the PAG1 sequence from 662 to 1240 was amplified by PCR with the primers 640down and 1240up on template pG-1240-CAT/EP2.

    Article Title: Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
    Article Snippet: .. For the RLFP assay, PCR products were digested with the restriction enzyme XbaI (Thermo Scientific). .. Cleaved DNA fragments were separated on 2 % agarose gels and the DNA concentration of each band was quantified using the ImageJ software.

    Generated:

    Article Title: Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis
    Article Snippet: .. Then the generated HspX-PPE44-EsxV, along with vector, was digested with BamHI and XbaI restriction enzymes and ligated into pcDNA3.1 (+) (Invitrogen, USA) in BamHI and XbaI restriction sites at the 5´and 3´ ends ( ). .. This plasmid was used to transform competent E. coli , Top-10 strain.

    Sequencing:

    Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei
    Article Snippet: .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240rev-CAT/EP2 (1240R ): the first 1240 bp of PAG1 were amplified by PCR from pG-1240-CAT/EP2 with the primers PAG1down3 and 1240up3 and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240-neo-CAT/EP2 (1240F -neo): the same strategy was used as for pG-640-neo-CAT/EP2, however the neo fragment was cloned into XbaI site of pG-1240-CAT/EP2. pG-PAG1end-CAT/EP2 (ORFF ): to obtain the fragment with the PAG1 sequence from its internal HindIII site at 1087 to position 2458, a PCR was performed with the template pBS-711-HN ( ) and the primers M13r (Invitrogen) and PAG1up2. .. The product was digested with HindIII and XbaI and cloned into the corresponding sites of pG-mcs-CAT/EP2. pG-PAG1end-rev-CAT/EP2 (ORFR ): the same strategy was used as for pG-PAG1end-CAT/EP2 using the primers PAGmid and PAG1up3. pG-662_1240-CAT/EP2 (MidF ): the PAG1 sequence from 662 to 1240 was amplified by PCR with the primers 640down and 1240up on template pG-1240-CAT/EP2.

    Plasmid Preparation:

    Article Title: Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei
    Article Snippet: .. The insert was excised with XhoI (cuts within the vector backbone 5′ to the PAG1 insert) and XbaI and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240rev-CAT/EP2 (1240R ): the first 1240 bp of PAG1 were amplified by PCR from pG-1240-CAT/EP2 with the primers PAG1down3 and 1240up3 and cloned into the XhoI and XbaI sites of pG-mcs-CAT/EP2. pG-1240-neo-CAT/EP2 (1240F -neo): the same strategy was used as for pG-640-neo-CAT/EP2, however the neo fragment was cloned into XbaI site of pG-1240-CAT/EP2. pG-PAG1end-CAT/EP2 (ORFF ): to obtain the fragment with the PAG1 sequence from its internal HindIII site at 1087 to position 2458, a PCR was performed with the template pBS-711-HN ( ) and the primers M13r (Invitrogen) and PAG1up2. .. The product was digested with HindIII and XbaI and cloned into the corresponding sites of pG-mcs-CAT/EP2. pG-PAG1end-rev-CAT/EP2 (ORFR ): the same strategy was used as for pG-PAG1end-CAT/EP2 using the primers PAGmid and PAG1up3. pG-662_1240-CAT/EP2 (MidF ): the PAG1 sequence from 662 to 1240 was amplified by PCR with the primers 640down and 1240up on template pG-1240-CAT/EP2.

    Article Title: Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis
    Article Snippet: .. Then the generated HspX-PPE44-EsxV, along with vector, was digested with BamHI and XbaI restriction enzymes and ligated into pcDNA3.1 (+) (Invitrogen, USA) in BamHI and XbaI restriction sites at the 5´and 3´ ends ( ). .. This plasmid was used to transform competent E. coli , Top-10 strain.