5 bb0002mutf kpni  (Thermo Fisher)


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  • 99
    Name:
    KpnI
    Description:
    5 G G T A C ↓C 3 3 C ↑C A T G G 5 Thermo Scientific KpnI restriction enzyme recognizes GGTAC C sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Isoschizomers Asp718I Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0523
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher 5 bb0002mutf kpni
    5 G G T A C ↓C 3 3 C ↑C A T G G 5 Thermo Scientific KpnI restriction enzyme recognizes GGTAC C sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Isoschizomers Asp718I Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/5 bb0002mutf kpni/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 bb0002mutf kpni - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Construction and Characterization of a 1,3-Propanediol Operon
    Article Snippet: .. This product was cut with Kpn I and Pst I and inserted into pSE380 (Invitrogen) cut with the same two enzymes to give pTC35. pTC35 was cut with Pst I and Sac I, both originally in the multiple cloning site of pSE380, so that the remainder of the dhaT gene (the Pst I- Sac I fragment of pTC3) could be inserted. .. The resulting plasmid contained the full dhaT gene under the control of the trc promoter.

    Article Title: Antiviral activity of recombinant ankyrin targeted to the capsid domain of HIV-1 Gag polyprotein
    Article Snippet: .. The PCR products of the second round were treated with Kpn I and Xho I (Fermentas) and cloned into corresponding sites of the pCEP4 vector. .. The sequence of AnkGAG 1D4-GFP and AnkA3 2D3-GFP, as well as all our other constructs, was verified by standard DNA sequencing.

    Synthesized:

    Article Title: Mucosal and systemic immune responses elicited by recombinant Lactococcus lactis expressing a fusion protein composed of pertussis toxin and filamentous hemagglutinin from Bordetella pertussis
    Article Snippet: .. The synthesized fragment (SECF1S1) was digested with Kpn I and Xba I restriction endonucleases (Thermo Fisher Scientific) and was inserted into the corresponding sites of the expression vector pNZ8149, giving rise to pNZ-SECF1S1. ..

    Sequencing:

    Article Title: PepGMV Rep-Protein Expression in Mammalian Cells
    Article Snippet: .. The insert presence was confirmed by double digestion with SpeI and KpnI enzymes (Fermentas).Gene correct orientation was confirmed by sequencing (Genetic Analyzer 3130, Applied Biosystems). .. Cell Culture and Transfection 3T3L1 mice fibroblast cells (ATCC No. CL-173) were grown in Dubelco’s Modified Eagle Medium (DMEM, Gibco) supplemented with penicillin (62.1 mg/L; Sigma), streptomycin (100 mg/L; Sigma), anphotericin (250 µg/L; Gibco), and 5% of calf serum (Biowest).

    Expressing:

    Article Title: Mucosal and systemic immune responses elicited by recombinant Lactococcus lactis expressing a fusion protein composed of pertussis toxin and filamentous hemagglutinin from Bordetella pertussis
    Article Snippet: .. The synthesized fragment (SECF1S1) was digested with Kpn I and Xba I restriction endonucleases (Thermo Fisher Scientific) and was inserted into the corresponding sites of the expression vector pNZ8149, giving rise to pNZ-SECF1S1. ..

    Polymerase Chain Reaction:

    Article Title: Antiviral activity of recombinant ankyrin targeted to the capsid domain of HIV-1 Gag polyprotein
    Article Snippet: .. The PCR products of the second round were treated with Kpn I and Xho I (Fermentas) and cloned into corresponding sites of the pCEP4 vector. .. The sequence of AnkGAG 1D4-GFP and AnkA3 2D3-GFP, as well as all our other constructs, was verified by standard DNA sequencing.

    Plasmid Preparation:

    Article Title: Antiviral activity of recombinant ankyrin targeted to the capsid domain of HIV-1 Gag polyprotein
    Article Snippet: .. The PCR products of the second round were treated with Kpn I and Xho I (Fermentas) and cloned into corresponding sites of the pCEP4 vector. .. The sequence of AnkGAG 1D4-GFP and AnkA3 2D3-GFP, as well as all our other constructs, was verified by standard DNA sequencing.

    Article Title: Antiviral activity of recombinant ankyrin targeted to the capsid domain of HIV-1 Gag polyprotein
    Article Snippet: .. This vector was first cleaved with Bsm B I and Kpn I (Fermentas) to generate the cohesive ends compatible with ankyrin repeats microgenes. .. The Kpn I cleavage, although not strictly necessary, was used to minimize the vector recircularization which would compete with ankyrin-repeat polymerisation.

    Article Title: PepGMV Rep-Protein Expression in Mammalian Cells
    Article Snippet: .. The pTracer™-SV40 vector (Invitrogen) was double digested with SpeI and KpnI enzymes (Fermentas) in order to linearize it and clone the Rep gene. .. Insert and vector purification was performed using the Silica Bead DNA Gel Extraction kit (Fermentas) and ligation reaction was done using the ligase T4 (Invitrogen) and a 4:1 insert/vector molar ratio.

    Article Title: Mucosal and systemic immune responses elicited by recombinant Lactococcus lactis expressing a fusion protein composed of pertussis toxin and filamentous hemagglutinin from Bordetella pertussis
    Article Snippet: .. The synthesized fragment (SECF1S1) was digested with Kpn I and Xba I restriction endonucleases (Thermo Fisher Scientific) and was inserted into the corresponding sites of the expression vector pNZ8149, giving rise to pNZ-SECF1S1. ..