ctni  (HyTest)


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    Name:
    Polyclonal anti cardiac troponin I cTnI
    Description:
    Cardiac markers
    Catalog Number:
    4T21/2
    Product Aliases:
    Anti-cTnI polyclonal
    Price:
    None
    Category:
    Polyclonal antibody
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    Structured Review

    HyTest ctni
    Polyclonal anti cardiac troponin I cTnI
    Cardiac markers
    https://www.bioz.com/result/ctni/product/HyTest
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ctni - by Bioz Stars, 2019-06
    96/100 stars

    Images

    1) Product Images from "Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction"

    Article Title: Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0136025

    E12.5. Early intramyocardial WT-1 positive cells. A-C. Control, transverse sections. B. E12.5 MLC-2a staining of right ventricle (RV) and left ventricle (LV) with complete epicardial covering and a visible subepicardial space in the LV and in the atrioventricular and interventricular sulcus (arrows). A, C: Enlargements of adjacent WT-1 stained sections of boxes in B, showing RV (A) and LV (C). The subepicardial layer is slightly broader on the LV side. WT-1+ cells are found in RV lateral wall (B, arrowheads) but not in the LV lateral wall (C). D-F: Fluorescent double stainings of WT-1 (green) and cTnI (red) at E12.5 confirm the first WT-1+ cells in the RV lateral wall, in the apical LV wall and the interventricular septum. G-L: WT1 CreERT2/+ , E12.5. There is a subepicardial layer in both ventricles with WT-1+ cells in the RV lateral wall (arrows in G and J), but not at the LV lateral wall (I and L). Some WT-1+ cells present at the base of both ventricles, and in the interventricular septum (IVS) (arrowheads in H). M-O: Tcf21 lacZ/+ mouse sections stained for LacZ in blue. N. LacZ staining of RV and LV comparable to B, showing epicardial covering and subepicardial space in the LV and in the atrioventricular and interventricular sulcus (arrows). M,O: Enlargements of boxed areas in E, showing RV (M) and LV (O). As in C, the subepicardial layer is slightly broader compared to the RV. No LacZ + cells where found in the RV lateral wall. Bars: B,E: 200μm, H, K: 500 μm, other bars: 50 μm.
    Figure Legend Snippet: E12.5. Early intramyocardial WT-1 positive cells. A-C. Control, transverse sections. B. E12.5 MLC-2a staining of right ventricle (RV) and left ventricle (LV) with complete epicardial covering and a visible subepicardial space in the LV and in the atrioventricular and interventricular sulcus (arrows). A, C: Enlargements of adjacent WT-1 stained sections of boxes in B, showing RV (A) and LV (C). The subepicardial layer is slightly broader on the LV side. WT-1+ cells are found in RV lateral wall (B, arrowheads) but not in the LV lateral wall (C). D-F: Fluorescent double stainings of WT-1 (green) and cTnI (red) at E12.5 confirm the first WT-1+ cells in the RV lateral wall, in the apical LV wall and the interventricular septum. G-L: WT1 CreERT2/+ , E12.5. There is a subepicardial layer in both ventricles with WT-1+ cells in the RV lateral wall (arrows in G and J), but not at the LV lateral wall (I and L). Some WT-1+ cells present at the base of both ventricles, and in the interventricular septum (IVS) (arrowheads in H). M-O: Tcf21 lacZ/+ mouse sections stained for LacZ in blue. N. LacZ staining of RV and LV comparable to B, showing epicardial covering and subepicardial space in the LV and in the atrioventricular and interventricular sulcus (arrows). M,O: Enlargements of boxed areas in E, showing RV (M) and LV (O). As in C, the subepicardial layer is slightly broader compared to the RV. No LacZ + cells where found in the RV lateral wall. Bars: B,E: 200μm, H, K: 500 μm, other bars: 50 μm.

    Techniques Used: Staining

    Related Articles

    Immunohistochemistry:

    Article Title: Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction
    Article Snippet: Paragraph title: Immunohistochemical procedures ... For immunofluorescence stainings, sections were incubated with primary antibodies against WT-1 (Wt1, clone 6F-H2, Millipore, CN-05-753 and 1/500 Abcam, ab899901) and cTnI (HyTest Ltd, CN-4T21_2) overnight at 4°C.

    Article Title: Development of the Hearts of Lizards and Snakes and Perspectives to Cardiac Evolution
    Article Snippet: Myocardial staining was performed as previously described . .. In all specimens of corn snake and sailfin lizard the myocardium was visualized by immunohistochemistry using a rabbit antibody to cardiac troponin I (cTnI) polyclonal antibody (HyTest ltd., dilution 1:500) binding of which was visualized by a fluorescently labelled secondary goat-anti-rabbit antibody coupled to Alexa 568 (Invitrogen, dilution 1:250). .. Two specimens of anole (stage 5 and 12) were cut in 7 µm sections and stained for the myocardial marker, cTnI, as described above, and were additionally stained for all nuclei with Sytox Green (1:40,000 Molecular Probes S-7020), and for incorporation of Bromodeoxyuridine (BrdU), a synthetic analogue of thymidine used to detect DNA replication, with a rat-monoclonal anti-BrdU (1:600, Immunosource).

    Amplification:

    Article Title: Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction
    Article Snippet: Slides were incubated with biotin-conjugated secondary antibody goat-anti-rabbit-biotin(1/200; Vector Laboratories, USA, BA-1000.The signal was amplified using ABC-reagent(Vector Laboratories, PK 6100), visualized with 3–3’di-aminobenzidine tetrahydrochloride(DAB, Sigma-Aldrich, D5637), and counterstained with 0.1% hematoxylin(Merck, Darmstadt, Germany). β-gal stainings were performed as previously described[ ]. .. For immunofluorescence stainings, sections were incubated with primary antibodies against WT-1 (Wt1, clone 6F-H2, Millipore, CN-05-753 and 1/500 Abcam, ab899901) and cTnI (HyTest Ltd, CN-4T21_2) overnight at 4°C.

    Article Title: Evolution and Development of Ventricular Septation in the Amniote Heart
    Article Snippet: Next, the sections were incubated with Tbx5 antibody (a kind gift from C.J.Hatcher, Ithaca, NY) and subsequently with horseradish peroxidase-conjugated secondary antibody, followed by Tyramide Signal Amplification (Perkin Elmer Life Science; #NEL700A). .. Subsequently, (sister) sections were incubated with antibodies directed against cardiac Troponin I (cTNI, HyTest Ltd, CN-4T21_2) followed by Alexa 555-conjugated secondary antibodies (Invitrogen, A-21432) to visualize the myocardium.

    Isolation:

    Article Title: Measurement and 3D-Visualization of Cell-Cycle Length Using Double Labelling with Two Thymidine Analogues Applied in Early Heart Development
    Article Snippet: After another hour, the embryos were isolated and washed in chicken physiological salt solution (0.719% NaCl). .. Each section was exposed overnight to a mixture of anti-IdU (mouse-monoclonal anti-BrdU; BD, 347580), anti-CldU (rat-monoclonal anti-BrdU; Serotec, OBT0030CX) and anti-cTnI (rabbit polyclonal; HyTest, 4T21/2) followed by incubation for at least 2 hrs with a mixture of the fluorescent antibodies, goat-anti-mouse-Alexa 680, goat-anti-rat-Alexa 568, goat-anti-rabbit-Alexa 405 (Invitrogen), and Sytox green 488 (Invitrogen).

    Cell Counting:

    Article Title: Measurement and 3D-Visualization of Cell-Cycle Length Using Double Labelling with Two Thymidine Analogues Applied in Early Heart Development
    Article Snippet: Each section was exposed overnight to a mixture of anti-IdU (mouse-monoclonal anti-BrdU; BD, 347580), anti-CldU (rat-monoclonal anti-BrdU; Serotec, OBT0030CX) and anti-cTnI (rabbit polyclonal; HyTest, 4T21/2) followed by incubation for at least 2 hrs with a mixture of the fluorescent antibodies, goat-anti-mouse-Alexa 680, goat-anti-rat-Alexa 568, goat-anti-rabbit-Alexa 405 (Invitrogen), and Sytox green 488 (Invitrogen). .. The myocardium was segmented based on cTnI expression; the dorsal mesoderm flanking the heart was manually segmented based on morphological landmarks.

    Immunofluorescence:

    Article Title: Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction
    Article Snippet: After overnight incubation at 3700B0C with 5-bromo-4chloro-3-indolyl-β-D-galactopyranoside (X-gal; 1mg/ml), hearts were stained against β-galactosidase (MP Biochemicals). .. For immunofluorescence stainings, sections were incubated with primary antibodies against WT-1 (Wt1, clone 6F-H2, Millipore, CN-05-753 and 1/500 Abcam, ab899901) and cTnI (HyTest Ltd, CN-4T21_2) overnight at 4°C. .. Tyramide Signal Amplification (PerkinElmer, CN- NEL749A001KT) was used to amplify the signal of the primary antibody against WT-1.

    Incubation:

    Article Title: Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction
    Article Snippet: After overnight incubation at 3700B0C with 5-bromo-4chloro-3-indolyl-β-D-galactopyranoside (X-gal; 1mg/ml), hearts were stained against β-galactosidase (MP Biochemicals). .. For immunofluorescence stainings, sections were incubated with primary antibodies against WT-1 (Wt1, clone 6F-H2, Millipore, CN-05-753 and 1/500 Abcam, ab899901) and cTnI (HyTest Ltd, CN-4T21_2) overnight at 4°C. .. Tyramide Signal Amplification (PerkinElmer, CN- NEL749A001KT) was used to amplify the signal of the primary antibody against WT-1.

    Article Title: Measurement and 3D-Visualization of Cell-Cycle Length Using Double Labelling with Two Thymidine Analogues Applied in Early Heart Development
    Article Snippet: Antigens were retrieved by 5 min of pressure cooking in antigen unmasking solution (Vector H3300). .. Each section was exposed overnight to a mixture of anti-IdU (mouse-monoclonal anti-BrdU; BD, 347580), anti-CldU (rat-monoclonal anti-BrdU; Serotec, OBT0030CX) and anti-cTnI (rabbit polyclonal; HyTest, 4T21/2) followed by incubation for at least 2 hrs with a mixture of the fluorescent antibodies, goat-anti-mouse-Alexa 680, goat-anti-rat-Alexa 568, goat-anti-rabbit-Alexa 405 (Invitrogen), and Sytox green 488 (Invitrogen). .. Image acquisition was performed with a fluorescent microscope using a 4 channel setup (Leica DM6000, Chromaphor).

    Article Title: Evolution and Development of Ventricular Septation in the Amniote Heart
    Article Snippet: Signal was visualized using Alexa 488-conjugated streptavidin (Invitrogen, S-11223). .. Subsequently, (sister) sections were incubated with antibodies directed against cardiac Troponin I (cTNI, HyTest Ltd, CN-4T21_2) followed by Alexa 555-conjugated secondary antibodies (Invitrogen, A-21432) to visualize the myocardium. .. Sections were mounted using ProLong Gold antifade reagent (Invitrogen, CN-P36930) with DAPI.

    Article Title: Development of the Hearts of Lizards and Snakes and Perspectives to Cardiac Evolution
    Article Snippet: In all specimens of corn snake and sailfin lizard the myocardium was visualized by immunohistochemistry using a rabbit antibody to cardiac troponin I (cTnI) polyclonal antibody (HyTest ltd., dilution 1:500) binding of which was visualized by a fluorescently labelled secondary goat-anti-rabbit antibody coupled to Alexa 568 (Invitrogen, dilution 1:250). .. 100 µl BrdU (10 mg BrdU (Sigma) per ml physiological salt solution (0.9% NaCl)) was injected through the shell into the egg yolk.

    Imaging:

    Article Title: Measurement and 3D-Visualization of Cell-Cycle Length Using Double Labelling with Two Thymidine Analogues Applied in Early Heart Development
    Article Snippet: Each section was exposed overnight to a mixture of anti-IdU (mouse-monoclonal anti-BrdU; BD, 347580), anti-CldU (rat-monoclonal anti-BrdU; Serotec, OBT0030CX) and anti-cTnI (rabbit polyclonal; HyTest, 4T21/2) followed by incubation for at least 2 hrs with a mixture of the fluorescent antibodies, goat-anti-mouse-Alexa 680, goat-anti-rat-Alexa 568, goat-anti-rabbit-Alexa 405 (Invitrogen), and Sytox green 488 (Invitrogen). .. Image acquisition was performed with a fluorescent microscope using a 4 channel setup (Leica DM6000, Chromaphor).

    Expressing:

    Article Title: Measurement and 3D-Visualization of Cell-Cycle Length Using Double Labelling with Two Thymidine Analogues Applied in Early Heart Development
    Article Snippet: Each section was exposed overnight to a mixture of anti-IdU (mouse-monoclonal anti-BrdU; BD, 347580), anti-CldU (rat-monoclonal anti-BrdU; Serotec, OBT0030CX) and anti-cTnI (rabbit polyclonal; HyTest, 4T21/2) followed by incubation for at least 2 hrs with a mixture of the fluorescent antibodies, goat-anti-mouse-Alexa 680, goat-anti-rat-Alexa 568, goat-anti-rabbit-Alexa 405 (Invitrogen), and Sytox green 488 (Invitrogen). .. Each section was exposed overnight to a mixture of anti-IdU (mouse-monoclonal anti-BrdU; BD, 347580), anti-CldU (rat-monoclonal anti-BrdU; Serotec, OBT0030CX) and anti-cTnI (rabbit polyclonal; HyTest, 4T21/2) followed by incubation for at least 2 hrs with a mixture of the fluorescent antibodies, goat-anti-mouse-Alexa 680, goat-anti-rat-Alexa 568, goat-anti-rabbit-Alexa 405 (Invitrogen), and Sytox green 488 (Invitrogen).

    Staining:

    Article Title: Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction
    Article Snippet: After overnight incubation at 3700B0C with 5-bromo-4chloro-3-indolyl-β-D-galactopyranoside (X-gal; 1mg/ml), hearts were stained against β-galactosidase (MP Biochemicals). .. For immunofluorescence stainings, sections were incubated with primary antibodies against WT-1 (Wt1, clone 6F-H2, Millipore, CN-05-753 and 1/500 Abcam, ab899901) and cTnI (HyTest Ltd, CN-4T21_2) overnight at 4°C.

    Article Title: Development of the Hearts of Lizards and Snakes and Perspectives to Cardiac Evolution
    Article Snippet: Paragraph title: Sectioning, staining, MRI scanning and 3D reconstructions ... In all specimens of corn snake and sailfin lizard the myocardium was visualized by immunohistochemistry using a rabbit antibody to cardiac troponin I (cTnI) polyclonal antibody (HyTest ltd., dilution 1:500) binding of which was visualized by a fluorescently labelled secondary goat-anti-rabbit antibody coupled to Alexa 568 (Invitrogen, dilution 1:250).

    Magnetic Resonance Imaging:

    Article Title: Development of the Hearts of Lizards and Snakes and Perspectives to Cardiac Evolution
    Article Snippet: Paragraph title: Sectioning, staining, MRI scanning and 3D reconstructions ... In all specimens of corn snake and sailfin lizard the myocardium was visualized by immunohistochemistry using a rabbit antibody to cardiac troponin I (cTnI) polyclonal antibody (HyTest ltd., dilution 1:500) binding of which was visualized by a fluorescently labelled secondary goat-anti-rabbit antibody coupled to Alexa 568 (Invitrogen, dilution 1:250).

    Binding Assay:

    Article Title: Development of the Hearts of Lizards and Snakes and Perspectives to Cardiac Evolution
    Article Snippet: Myocardial staining was performed as previously described . .. In all specimens of corn snake and sailfin lizard the myocardium was visualized by immunohistochemistry using a rabbit antibody to cardiac troponin I (cTnI) polyclonal antibody (HyTest ltd., dilution 1:500) binding of which was visualized by a fluorescently labelled secondary goat-anti-rabbit antibody coupled to Alexa 568 (Invitrogen, dilution 1:250). .. Two specimens of anole (stage 5 and 12) were cut in 7 µm sections and stained for the myocardial marker, cTnI, as described above, and were additionally stained for all nuclei with Sytox Green (1:40,000 Molecular Probes S-7020), and for incorporation of Bromodeoxyuridine (BrdU), a synthetic analogue of thymidine used to detect DNA replication, with a rat-monoclonal anti-BrdU (1:600, Immunosource).

    Injection:

    Article Title: Measurement and 3D-Visualization of Cell-Cycle Length Using Double Labelling with Two Thymidine Analogues Applied in Early Heart Development
    Article Snippet: After 3 hours, an injection with 100 µl of CldU solution followed. .. Each section was exposed overnight to a mixture of anti-IdU (mouse-monoclonal anti-BrdU; BD, 347580), anti-CldU (rat-monoclonal anti-BrdU; Serotec, OBT0030CX) and anti-cTnI (rabbit polyclonal; HyTest, 4T21/2) followed by incubation for at least 2 hrs with a mixture of the fluorescent antibodies, goat-anti-mouse-Alexa 680, goat-anti-rat-Alexa 568, goat-anti-rabbit-Alexa 405 (Invitrogen), and Sytox green 488 (Invitrogen).

    Article Title: Development of the Hearts of Lizards and Snakes and Perspectives to Cardiac Evolution
    Article Snippet: In all specimens of corn snake and sailfin lizard the myocardium was visualized by immunohistochemistry using a rabbit antibody to cardiac troponin I (cTnI) polyclonal antibody (HyTest ltd., dilution 1:500) binding of which was visualized by a fluorescently labelled secondary goat-anti-rabbit antibody coupled to Alexa 568 (Invitrogen, dilution 1:250). .. Antibody binding was then visualized using a fluorescently labelled secondary goat-anti-rat antibody coupled to Alexa 680 (Invitrogen, dilution 1:250).

    Plasmid Preparation:

    Article Title: Measurement and 3D-Visualization of Cell-Cycle Length Using Double Labelling with Two Thymidine Analogues Applied in Early Heart Development
    Article Snippet: Each section was exposed overnight to a mixture of anti-IdU (mouse-monoclonal anti-BrdU; BD, 347580), anti-CldU (rat-monoclonal anti-BrdU; Serotec, OBT0030CX) and anti-cTnI (rabbit polyclonal; HyTest, 4T21/2) followed by incubation for at least 2 hrs with a mixture of the fluorescent antibodies, goat-anti-mouse-Alexa 680, goat-anti-rat-Alexa 568, goat-anti-rabbit-Alexa 405 (Invitrogen), and Sytox green 488 (Invitrogen). .. Each section was exposed overnight to a mixture of anti-IdU (mouse-monoclonal anti-BrdU; BD, 347580), anti-CldU (rat-monoclonal anti-BrdU; Serotec, OBT0030CX) and anti-cTnI (rabbit polyclonal; HyTest, 4T21/2) followed by incubation for at least 2 hrs with a mixture of the fluorescent antibodies, goat-anti-mouse-Alexa 680, goat-anti-rat-Alexa 568, goat-anti-rabbit-Alexa 405 (Invitrogen), and Sytox green 488 (Invitrogen).

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  • 96
    HyTest mouse anti ctni mabs 19c7
    The <t>anti-cTnI</t> <t>mAbs</t> could stain HEK 293 cells and LO2 cells by IF. A-D: Representative confocal images of HEK 293 cells (bar = 40 μm). A: Negative control. B-D: HEK 293 cells were stained for cTnI (green) using 2F6.6, XY15 and XY10 mAbs, which
    Mouse Anti Ctni Mabs 19c7, supplied by HyTest, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ctni mabs 19c7/product/HyTest
    Average 96 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mouse anti ctni mabs 19c7 - by Bioz Stars, 2019-06
    96/100 stars
      Buy from Supplier

    93
    HyTest polyclonal anti cardiac troponin i ctni
    Myocardial Proliferation but No Regeneration in Pachón Hearts (A and B) No significant difference in the number of PCNA/Mef2-positive cells surrounding the wound in Pachón compared with surface fish hearts (A). Myocardial proliferation is highest at 7 dpa in both fish (B). n ≥ 4 per population per time point, two-way ANOVA with Sidak or Tukey test. (C and D) Twenty-four-hour BrdU administration at 6 dpa with heart isolation at 7 dpa or 24 hr BrdU at 7 dpa with isolation at 14 dpa (C). No difference in the number of BrdU-positive cells at 7 dpa (Pachón n = 5, surface fish n = 4) but reduced labeling in the Pachón (n = 6) compared with surface fish (n = 6) at 14 dpa (D). Similar number of cells labeled in Pachón at 7 and 14 dpa. One-way ANOVA with Tukey’s test. (E) Comparable low levels of CC3-positive cells in surface fish and Pachón. <t>cTnI,</t> cardiac troponin I. (F–K) PCNA counts on the basal side of the ventricle. n ≥ 4 per population per time point, two-way ANOVA with Sidak or Tukey test. Increased non-myocardial proliferation at the epicardial layer after injury in the Pachón compared to surface fish (F), and representative image of non-myocardial proliferation 7dpa, indicated by PCNA-positive/Mef2-negative cells at the epicardial layer (G). Sharp increase in non-myocardial proliferation at 14 dpa in the luminal cells of the ventricle in the Pachón versus surface fish (H), with representative image of non-myocardial proliferation 14 dpa in the trabecular area/luminal side (I). Increased myocardial proliferation on the other side of the ventricle in Pachón compared to surface fish at 30 dpa (J), with representative image of myocardial proliferation basal side indicated by PCNA/Mef2-positive cells at 30 dpa in surface fish and Pachón (K). (L and M) Increased non-myocardial cells in the Pachón heart at 30 dpa (L). Representative DAPI and mef2 staining in Pachón and surface fish at the base of the ventricle, 30 dpa (M). n = 4 per fish per time point, two-way ANOVA with Sidak or Tukey test. (N and O) Proliferation in the non-myocardial luminal cells is mostly located in Erg1-positive endocardial cells (N), followed by an increase in endocardial cells in Pachón at 30 dpa (O). Unpaired, two-tailed, equal-variance t test, p = 0.0274. Detailed numbers and statistics are provided in STAR Methods . Results are presented as mean ± SEM. All scale bars, 100 μm. CW, compact wall; Lu, lumen.
    Polyclonal Anti Cardiac Troponin I Ctni, supplied by HyTest, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti cardiac troponin i ctni/product/HyTest
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    polyclonal anti cardiac troponin i ctni - by Bioz Stars, 2019-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    The anti-cTnI mAbs could stain HEK 293 cells and LO2 cells by IF. A-D: Representative confocal images of HEK 293 cells (bar = 40 μm). A: Negative control. B-D: HEK 293 cells were stained for cTnI (green) using 2F6.6, XY15 and XY10 mAbs, which

    Journal:

    Article Title: Cardiac troponin I is abnormally expressed in non-small cell lung cancer tissues and human cancer cells

    doi:

    Figure Lengend Snippet: The anti-cTnI mAbs could stain HEK 293 cells and LO2 cells by IF. A-D: Representative confocal images of HEK 293 cells (bar = 40 μm). A: Negative control. B-D: HEK 293 cells were stained for cTnI (green) using 2F6.6, XY15 and XY10 mAbs, which

    Article Snippet: The mouse anti-cTnI mAbs 19C7, 16A11 and 84 were purchased from Hytest (Turku, Finland).

    Techniques: Staining, Negative Control

    The anti-cTnI mAbs could stain Huh-7 cells, MHCC-97L cells and MHCC-97H cells by IF. A-C: Representative confocal images of Huh-7 cells (bar = 40 μm). A: Negative control. B, C: Huh-7 cells were stained for cTnI (Fluorescein Avidin DCS, green)

    Journal:

    Article Title: Cardiac troponin I is abnormally expressed in non-small cell lung cancer tissues and human cancer cells

    doi:

    Figure Lengend Snippet: The anti-cTnI mAbs could stain Huh-7 cells, MHCC-97L cells and MHCC-97H cells by IF. A-C: Representative confocal images of Huh-7 cells (bar = 40 μm). A: Negative control. B, C: Huh-7 cells were stained for cTnI (Fluorescein Avidin DCS, green)

    Article Snippet: The mouse anti-cTnI mAbs 19C7, 16A11 and 84 were purchased from Hytest (Turku, Finland).

    Techniques: Staining, Negative Control, Avidin-Biotin Assay

    The anti-cTnI mAbs could stain different parts of BGC 823 cells by immunofluorescence with an epitope-dependent pattern. Representative confocal images of BGC 823 cells (bar = 40 μm). A: Negative control. B-H: BGC 823 cells were stained for cTnI

    Journal:

    Article Title: Cardiac troponin I is abnormally expressed in non-small cell lung cancer tissues and human cancer cells

    doi:

    Figure Lengend Snippet: The anti-cTnI mAbs could stain different parts of BGC 823 cells by immunofluorescence with an epitope-dependent pattern. Representative confocal images of BGC 823 cells (bar = 40 μm). A: Negative control. B-H: BGC 823 cells were stained for cTnI

    Article Snippet: The mouse anti-cTnI mAbs 19C7, 16A11 and 84 were purchased from Hytest (Turku, Finland).

    Techniques: Staining, Immunofluorescence, Negative Control

    The anti-cTnI mAbs could stain different parts of SPCA-1 cells by immunofluorescence with an epitope-dependent pattern. Representative confocal images of SPCA-1 cells (bar = 40 μm). A: Negative control. B-H: SPCA-1 cells were stained for cTnI

    Journal:

    Article Title: Cardiac troponin I is abnormally expressed in non-small cell lung cancer tissues and human cancer cells

    doi:

    Figure Lengend Snippet: The anti-cTnI mAbs could stain different parts of SPCA-1 cells by immunofluorescence with an epitope-dependent pattern. Representative confocal images of SPCA-1 cells (bar = 40 μm). A: Negative control. B-H: SPCA-1 cells were stained for cTnI

    Article Snippet: The mouse anti-cTnI mAbs 19C7, 16A11 and 84 were purchased from Hytest (Turku, Finland).

    Techniques: Staining, Immunofluorescence, Negative Control

    Vangl2 is expressed in the distal outflow region. A ) Cartoon showing the region encompassing the dorsal pericardial wall and the distal outflow tract, including the region we describe as the transition zone.  B–F ) Vangl2 protein (red), labelled by immunofluorescence, is expressed in the distal outflow region (B), localising to the basal part of the membrane of the cells (as shown by co-localisation with β-catenin, a baso-lateral marker; green) in the dorsal pericardial wall and transition zone (B,C,E), but is found diffusely in the cytoplasm more proximally (B,D,F).  G–H ) Cardiac troponin I staining (red; labelling cardiomyocytes) is initially weak within the distal outflow but is upregulated more proximally (I). Vangl2 (green) and cardiac troponin I are co-expressed in the transition zone (J - TZ and arrows) of the outflow tract with the membrane-localisation of Vangl2 gradually lost (H) as cardiac troponin I staining becomes stronger.  K–N ) Vangl2 and Isl1 are also co-expressed in the cells of the transition zone (N - TZ and arrows), with the loss of Vangl2 from the membrane proximally coinciding with the loss of nuclear Isl1 localisation (N - lower white arrowhead). All images shown are of Vangl2 f/+  embryos. A =  Apical, B =  Basal, D =  distal, endo  =  endocardium, myo  =  myocardium, P =  proximal, TZ =  transition zone. Scale bar  = 25 µm.

    Journal: PLoS Genetics

    Article Title: Vangl2-Regulated Polarisation of Second Heart Field-Derived Cells Is Required for Outflow Tract Lengthening during Cardiac Development

    doi: 10.1371/journal.pgen.1004871

    Figure Lengend Snippet: Vangl2 is expressed in the distal outflow region. A ) Cartoon showing the region encompassing the dorsal pericardial wall and the distal outflow tract, including the region we describe as the transition zone. B–F ) Vangl2 protein (red), labelled by immunofluorescence, is expressed in the distal outflow region (B), localising to the basal part of the membrane of the cells (as shown by co-localisation with β-catenin, a baso-lateral marker; green) in the dorsal pericardial wall and transition zone (B,C,E), but is found diffusely in the cytoplasm more proximally (B,D,F). G–H ) Cardiac troponin I staining (red; labelling cardiomyocytes) is initially weak within the distal outflow but is upregulated more proximally (I). Vangl2 (green) and cardiac troponin I are co-expressed in the transition zone (J - TZ and arrows) of the outflow tract with the membrane-localisation of Vangl2 gradually lost (H) as cardiac troponin I staining becomes stronger. K–N ) Vangl2 and Isl1 are also co-expressed in the cells of the transition zone (N - TZ and arrows), with the loss of Vangl2 from the membrane proximally coinciding with the loss of nuclear Isl1 localisation (N - lower white arrowhead). All images shown are of Vangl2 f/+ embryos. A =  Apical, B =  Basal, D =  distal, endo  =  endocardium, myo  =  myocardium, P =  proximal, TZ =  transition zone. Scale bar  = 25 µm.

    Article Snippet: Samples were blocked in 10% FCS and then incubated either overnight at 4°C, or at room temperature for 2 hours with the following antibodies: E-cadherin, β-catenin, N-Cadherin (BD Transduction Laboratories), fibronectin, Scrib, PKCζ (Santa Cruz), Isl1, MF20 (Developmental studies Hybridoma Bank, University of Iowa), GFP, alpha smooth muscle actin (Abcam), gamma tubulin, laminin (Sigma), cardiac troponin I (HyTest), desmin (Millipore).

    Techniques: Immunofluorescence, Marker, Staining

    Loss of Vangl2 results in loss of SHF progenitor phenotype and premature differentiation in the distal outflow tract of  Vangl2 flox/flox ; Isl1-Cre  embryos. A–F ) At E9.0, cardiac troponin I expression is low distally and increases proximally through the outflow tract of control embryos (A). In contrast, high-level expression is found more distally in  Vangl2 flox/flox ; Isl1-Cre  embryos (D, n = 2). Desmin, which is expressed at high level in cardiomyocytes and at lower level by smooth muscle cells (B) is also increased within the distal outflow tract of  Vangl2 flox/flox ; Isl1-Cre  embryos (E, n = 3). Whereas Isl1 is localised to the nucleus of control embryos throughout an extended region of the distal outflow tract, defining the transition zone (C - arrows), it is significantly reduced in the nuclei of cells in the distal outflow of  Vangl2 flox/flox ; Isl1-Cre  embryos (F – arrows point to the proximal extent of the staining, n = 3). G–J) Similar to E9.0, at E10.5, both αSMA (G,I, n = 3) and MF20 (H,J; staining myosin heavy chain, n = 3) are expressed more distally in the outflow tract of  Vangl2 flox/flox ; Isl1-Cre  embryos than in stage-matched littermates. K,L) Cartoon showing distribution of Vangl2 (bright green) at the boundary of the transition zone in the distal outflow tract of control embryos, where it is localised to the membrane through the transition zone, but is cytoplasmic (green stars) more proximally. Basolateral markers are represented in red and the MTOC, localising to the apical side of the cell, in dark green (K). In the absence of Vangl2, basolateral marker domains are expanded and the MTOC, although still apically positioned, is rotated in many cells. The wall is also thickened (L). M) Model showing how loss of epithelial phenotype of the cells within the distal outflow tract wall at E9.5 could result in a shortened outflow tract and double outlet right ventricle by E14.5. A =  Apical, B =  Basal, D =  distal, P =  proximal,  Vangl2 f   =   Vangl2 flox . Scale bar  = 100 µm.

    Journal: PLoS Genetics

    Article Title: Vangl2-Regulated Polarisation of Second Heart Field-Derived Cells Is Required for Outflow Tract Lengthening during Cardiac Development

    doi: 10.1371/journal.pgen.1004871

    Figure Lengend Snippet: Loss of Vangl2 results in loss of SHF progenitor phenotype and premature differentiation in the distal outflow tract of Vangl2 flox/flox ; Isl1-Cre embryos. A–F ) At E9.0, cardiac troponin I expression is low distally and increases proximally through the outflow tract of control embryos (A). In contrast, high-level expression is found more distally in Vangl2 flox/flox ; Isl1-Cre embryos (D, n = 2). Desmin, which is expressed at high level in cardiomyocytes and at lower level by smooth muscle cells (B) is also increased within the distal outflow tract of Vangl2 flox/flox ; Isl1-Cre embryos (E, n = 3). Whereas Isl1 is localised to the nucleus of control embryos throughout an extended region of the distal outflow tract, defining the transition zone (C - arrows), it is significantly reduced in the nuclei of cells in the distal outflow of Vangl2 flox/flox ; Isl1-Cre embryos (F – arrows point to the proximal extent of the staining, n = 3). G–J) Similar to E9.0, at E10.5, both αSMA (G,I, n = 3) and MF20 (H,J; staining myosin heavy chain, n = 3) are expressed more distally in the outflow tract of Vangl2 flox/flox ; Isl1-Cre embryos than in stage-matched littermates. K,L) Cartoon showing distribution of Vangl2 (bright green) at the boundary of the transition zone in the distal outflow tract of control embryos, where it is localised to the membrane through the transition zone, but is cytoplasmic (green stars) more proximally. Basolateral markers are represented in red and the MTOC, localising to the apical side of the cell, in dark green (K). In the absence of Vangl2, basolateral marker domains are expanded and the MTOC, although still apically positioned, is rotated in many cells. The wall is also thickened (L). M) Model showing how loss of epithelial phenotype of the cells within the distal outflow tract wall at E9.5 could result in a shortened outflow tract and double outlet right ventricle by E14.5. A =  Apical, B =  Basal, D =  distal, P =  proximal, Vangl2 f  =  Vangl2 flox . Scale bar  = 100 µm.

    Article Snippet: Samples were blocked in 10% FCS and then incubated either overnight at 4°C, or at room temperature for 2 hours with the following antibodies: E-cadherin, β-catenin, N-Cadherin (BD Transduction Laboratories), fibronectin, Scrib, PKCζ (Santa Cruz), Isl1, MF20 (Developmental studies Hybridoma Bank, University of Iowa), GFP, alpha smooth muscle actin (Abcam), gamma tubulin, laminin (Sigma), cardiac troponin I (HyTest), desmin (Millipore).

    Techniques: Expressing, Staining, Marker

    Detection of myocardial necrosis, endothelial dysfunction and inflammation in blood serum. Blood serum samples obtained at baseline, 50min after ischemia, 10min, 1hour and 2hours after reperfusion were analyzed. for myocardial necrosis by measuring cardiac troponin-I. Endothelial dysfunction was analyzed by measuring CD31 protein levels and inflammation was analyzed by measuring MCP-1, C3b/c and C5b9 levels.

    Journal: PLoS ONE

    Article Title: Dexrazoxane Shows No Protective Effect in the Acute Phase of Reperfusion during Myocardial Infarction in Pigs

    doi: 10.1371/journal.pone.0168541

    Figure Lengend Snippet: Detection of myocardial necrosis, endothelial dysfunction and inflammation in blood serum. Blood serum samples obtained at baseline, 50min after ischemia, 10min, 1hour and 2hours after reperfusion were analyzed. for myocardial necrosis by measuring cardiac troponin-I. Endothelial dysfunction was analyzed by measuring CD31 protein levels and inflammation was analyzed by measuring MCP-1, C3b/c and C5b9 levels.

    Article Snippet: Beads were washed three times, and incubated together with a cocktail of detection antibodies: anti cTn- I-biotin (pAb, HyTest), anti CD31-biotin (mAb, AbD Serotec), anti MCP-1-biotin (pAb, PeproTech), anti C3b/c-FITC (pAb, Dako) and anti C6-biotin (mAb, Quidel) in a final volume 25 μl/well.

    Techniques:

    Myocardial Proliferation but No Regeneration in Pachón Hearts (A and B) No significant difference in the number of PCNA/Mef2-positive cells surrounding the wound in Pachón compared with surface fish hearts (A). Myocardial proliferation is highest at 7 dpa in both fish (B). n ≥ 4 per population per time point, two-way ANOVA with Sidak or Tukey test. (C and D) Twenty-four-hour BrdU administration at 6 dpa with heart isolation at 7 dpa or 24 hr BrdU at 7 dpa with isolation at 14 dpa (C). No difference in the number of BrdU-positive cells at 7 dpa (Pachón n = 5, surface fish n = 4) but reduced labeling in the Pachón (n = 6) compared with surface fish (n = 6) at 14 dpa (D). Similar number of cells labeled in Pachón at 7 and 14 dpa. One-way ANOVA with Tukey’s test. (E) Comparable low levels of CC3-positive cells in surface fish and Pachón. cTnI, cardiac troponin I. (F–K) PCNA counts on the basal side of the ventricle. n ≥ 4 per population per time point, two-way ANOVA with Sidak or Tukey test. Increased non-myocardial proliferation at the epicardial layer after injury in the Pachón compared to surface fish (F), and representative image of non-myocardial proliferation 7dpa, indicated by PCNA-positive/Mef2-negative cells at the epicardial layer (G). Sharp increase in non-myocardial proliferation at 14 dpa in the luminal cells of the ventricle in the Pachón versus surface fish (H), with representative image of non-myocardial proliferation 14 dpa in the trabecular area/luminal side (I). Increased myocardial proliferation on the other side of the ventricle in Pachón compared to surface fish at 30 dpa (J), with representative image of myocardial proliferation basal side indicated by PCNA/Mef2-positive cells at 30 dpa in surface fish and Pachón (K). (L and M) Increased non-myocardial cells in the Pachón heart at 30 dpa (L). Representative DAPI and mef2 staining in Pachón and surface fish at the base of the ventricle, 30 dpa (M). n = 4 per fish per time point, two-way ANOVA with Sidak or Tukey test. (N and O) Proliferation in the non-myocardial luminal cells is mostly located in Erg1-positive endocardial cells (N), followed by an increase in endocardial cells in Pachón at 30 dpa (O). Unpaired, two-tailed, equal-variance t test, p = 0.0274. Detailed numbers and statistics are provided in STAR Methods . Results are presented as mean ± SEM. All scale bars, 100 μm. CW, compact wall; Lu, lumen.

    Journal: Cell Reports

    Article Title: Heart Regeneration in the Mexican Cavefish

    doi: 10.1016/j.celrep.2018.10.072

    Figure Lengend Snippet: Myocardial Proliferation but No Regeneration in Pachón Hearts (A and B) No significant difference in the number of PCNA/Mef2-positive cells surrounding the wound in Pachón compared with surface fish hearts (A). Myocardial proliferation is highest at 7 dpa in both fish (B). n ≥ 4 per population per time point, two-way ANOVA with Sidak or Tukey test. (C and D) Twenty-four-hour BrdU administration at 6 dpa with heart isolation at 7 dpa or 24 hr BrdU at 7 dpa with isolation at 14 dpa (C). No difference in the number of BrdU-positive cells at 7 dpa (Pachón n = 5, surface fish n = 4) but reduced labeling in the Pachón (n = 6) compared with surface fish (n = 6) at 14 dpa (D). Similar number of cells labeled in Pachón at 7 and 14 dpa. One-way ANOVA with Tukey’s test. (E) Comparable low levels of CC3-positive cells in surface fish and Pachón. cTnI, cardiac troponin I. (F–K) PCNA counts on the basal side of the ventricle. n ≥ 4 per population per time point, two-way ANOVA with Sidak or Tukey test. Increased non-myocardial proliferation at the epicardial layer after injury in the Pachón compared to surface fish (F), and representative image of non-myocardial proliferation 7dpa, indicated by PCNA-positive/Mef2-negative cells at the epicardial layer (G). Sharp increase in non-myocardial proliferation at 14 dpa in the luminal cells of the ventricle in the Pachón versus surface fish (H), with representative image of non-myocardial proliferation 14 dpa in the trabecular area/luminal side (I). Increased myocardial proliferation on the other side of the ventricle in Pachón compared to surface fish at 30 dpa (J), with representative image of myocardial proliferation basal side indicated by PCNA/Mef2-positive cells at 30 dpa in surface fish and Pachón (K). (L and M) Increased non-myocardial cells in the Pachón heart at 30 dpa (L). Representative DAPI and mef2 staining in Pachón and surface fish at the base of the ventricle, 30 dpa (M). n = 4 per fish per time point, two-way ANOVA with Sidak or Tukey test. (N and O) Proliferation in the non-myocardial luminal cells is mostly located in Erg1-positive endocardial cells (N), followed by an increase in endocardial cells in Pachón at 30 dpa (O). Unpaired, two-tailed, equal-variance t test, p = 0.0274. Detailed numbers and statistics are provided in STAR Methods . Results are presented as mean ± SEM. All scale bars, 100 μm. CW, compact wall; Lu, lumen.

    Article Snippet: The following primary antibodies were used: goat polyclonal against Cardiac Troponin I (cTnI, 1:200, Hytest, 4T21/2), rabbit polyclonal against Myocyte Enhancer Factor 2 (Mef2 C-21, 1:200, Santa Cruz, sc-313), Cleaved Caspase-3 (CC3 Asp175, 1:200; Cell Signaling Technology, 9661S), and mouse monoclonal antibodies against 5-bromo-2′-deoxyuridine (BrdU BU5.1, 1:200.

    Techniques: Fluorescence In Situ Hybridization, Isolation, Labeling, Staining, Two Tailed Test