mouse breast cancer cell line 4t1  (ATCC)

Journal: Scientific Reports

Article Title: Retarding breast tumor growth with nanoparticle-facilitated intravenous delivery of BRCA1 and BRCA2 tumor suppressor genes

doi: 10.1038/s41598-022-25511-9

Figure Lengend Snippet: Cell viability assessment of BRCA1 and BRCA2 plasmid delivery in (a) MCF-7 (b) MDA-MB-231 and (c) 4T1 cells treated with BRCA1 + NP, and BRCA2 + NP formulations for 48 h. The experiments were performed three times in each of the cell lines and values were presented as mean ± SD of triplicates in MTT assay. * indicated p < 0.05 compared to NPs control.

Article Snippet: Two human breast cancer cell lines MCF-7, and MDA-MB-231, one mouse breast cancer cell line 4T1 were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Plasmid Preparation, MTT Assay

Journal: Scientific Reports

Article Title: Retarding breast tumor growth with nanoparticle-facilitated intravenous delivery of BRCA1 and BRCA2 tumor suppressor genes

doi: 10.1038/s41598-022-25511-9

Figure Lengend Snippet: Cytotoxicity imposed by BRCA1 + NP and BRCA2 + NP formulations in three breast cancer cell lines.

Article Snippet: Two human breast cancer cell lines MCF-7, and MDA-MB-231, one mouse breast cancer cell line 4T1 were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques:

Journal: Scientific Reports

Article Title: Retarding breast tumor growth with nanoparticle-facilitated intravenous delivery of BRCA1 and BRCA2 tumor suppressor genes

doi: 10.1038/s41598-022-25511-9

Figure Lengend Snippet: Light microscopic images of two different cell lines following treatment with NP, BRCA1 + NP and BRCA2 + NP. Cells treated with BRCA1 + NP and BRCA2 + NP, revealed decrease in cellular density in MCF-7 (upper panel) and 4T1 (lower panel) cell line, compared to the untreated control cells (concentrated).

Article Snippet: Two human breast cancer cell lines MCF-7, and MDA-MB-231, one mouse breast cancer cell line 4T1 were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques:

Journal: Scientific Reports

Article Title: Retarding breast tumor growth with nanoparticle-facilitated intravenous delivery of BRCA1 and BRCA2 tumor suppressor genes

doi: 10.1038/s41598-022-25511-9

Figure Lengend Snippet: Tumor regression study following intravenous delivery of NP, BRCA1 + NP, and BRCA2 + NP in 4T1 cells-induced tumor mouse model. 4T1 cells were inoculated subcutaneously on the mammary pad of mice. On day 8, as the tumor reached an approximate volume of ~ 25 mm 3 , mice were treated intravenously through tail-vein injection with 100 μL of NP (8 M Ca 2+ ), BRCA1 + NP (40 µg of plasmid) and BRCA2 + NP (40 µg of plasmid); followed by the 2nd dose at day 10. The tumor outgrowth was monitored until day 22. Five mice were used per group and data were represented as mean ± SD. Compared to NP control group, statistical analysis was significant, * when p < 0.05. (* p < 0.05 (NP control and BRCA1 + NP), * p < 0.05 (NP control and BRCA2 + NP).

Article Snippet: Two human breast cancer cell lines MCF-7, and MDA-MB-231, one mouse breast cancer cell line 4T1 were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Injection, Plasmid Preparation

Journal: Scientific Reports

Article Title: Retarding breast tumor growth with nanoparticle-facilitated intravenous delivery of BRCA1 and BRCA2 tumor suppressor genes

doi: 10.1038/s41598-022-25511-9

Figure Lengend Snippet: Transgene delivery of BRCA1 + NP and BRCA2 + NP in 4T1-induced tumor mouse model inhibited tumor growth. ( a ) Tumor images captured following sacrifice at day 22. ( b ) Quantitation of tumor volumes in NP control, BRCA1 plasmid-treated mice and BRCA2 plasmid-treated mice. ( c ) Quantitation of tumor weights. Statistical analysis was found significant compared to NP control group, * when p < 0.05.

Article Snippet: Two human breast cancer cell lines MCF-7, and MDA-MB-231, one mouse breast cancer cell line 4T1 were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Quantitation Assay, Plasmid Preparation

Journal: Nature Communications

Article Title: Reprogramming the tumor immune microenvironment using engineered dual-drug loaded Salmonella

doi: 10.1038/s41467-024-50950-5

Figure Lengend Snippet: a Experimental scheme. Briefly, 4T1 cells were implanted into the mammary fat pad of BALB/c mice. When tumors reached approximately 140 mm 3 , mice received bacteria through i.v injection (day 0). Doxy (+) and CSF-1R antibody (αCSF-1R) were administered as shown. Pimary tumors and metastatic lungs were collected on day 15 and 35, respectively ( b , c ) and flow cytometry was performed on day 9 ( d , e ). b Average growth curves of 4T1 primary tumors ( n = 6 mice examined from two independent experimental replicates; * P = 0.0144, **** P < 0.0001; two-way ANOVA with Tukey’s multiple comparisons test). c Individual images (left) and metastatic nodule count (right) of lungs ( n = 6 exami n ed from two independent experimental replicates; **** P < 0.0001; ns not significant; unpaired two-tailed t -test). d Flow cytome t ry gating of M1 (F4/80 + CD86 + ) and M2 (F4/80 + CD206 + ) macrophages . e Ratio of M1/M2 macrophages in lungs (left) and tumors (right) ( n = 6 mice examined from two independent experimental replicates; **** P < 0.0001; ns, not significant; unpaired two-tailed t -test). f In vivo and ex vivo bioluminescent imaging of MDA-MB-231-FLuc-GFP lung metastasis. Left, images of the mice on day 0 (pre-treatment) and on day 14 (post-treatment) and the excised lungs on day 14; right, quantitation of bioluminescence levels in the lungs. g Frequency of macrophages phagocytosing tumor cells (F4/80 + GFP + ) in the metastatic lungs of ( f ) ( n = 3 mice/group, from one experiment, unpaired two-tailed t -test). h Experimental scheme. i Average growth curves of subcutaneous tumors ( n = 7 mice examined from two independent experimental replicates; **** P < 0.0001; two-way ANOVA with Tukey’s multiple comparisons test). j Kaplan-Meier survival curves ( n = 7 mice examined from two independent experimental replicates; **** P < 0.0001; Log-rank (Mantel-Cox) test). k Metastatic scores of livers. Metastatic nodules on the liver surface were counted at the experimental end point. The scores were classified as follows: 0, 0%; 1, <20%; 2, 20%–50%; 3, >50% ( n = 7 mice examined from two independent experimental replicates; **** P < 0.0001; unpaired two-tailed t -test).

Article Snippet: CT26 (mouse colon carcinoma, CRL-2638), 4T1 (mouse breast cancer, CRL-2539), and HEK293T (human epithelial cell, CRL-3216) cell lines were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Bacteria, Injection, Flow Cytometry, Two Tailed Test, In Vivo, Ex Vivo, Imaging, Quantitation Assay

Journal: Pharmaceutics

Article Title: Study of Cytotoxicity and Internalization of Redox-Responsive Iron Oxide Nanoparticles on PC-3 and 4T1 Cancer Cell Lines

doi: 10.3390/pharmaceutics15010127

Figure Lengend Snippet: Evaluation of cytotoxicity of MNP-PEG and MNP-SS on PC-3 ( A ) and 4T1 ( B ) cells. Differences were considered statistically significant at: *— p < 0.05, **— p < 0.01, ***— p < 0.001.

Article Snippet: PC-3 human prostate cancer cells and 4T1 mouse breast cancer cells (American Type Culture Collection, Manassas, VA, USA) were cultured in a 5% CO 2 atmosphere at 37 °C in DMEM/F12 medium containing 10% fetal bovine serum (Sigma-Aldrich, Burlington, VT, USA), 1% L-glutamine (Gibco, Waltham, MA, USA), and 1% antibiotics (penicillin and streptomycin).

Techniques:

Journal: Pharmaceutics

Article Title: Study of Cytotoxicity and Internalization of Redox-Responsive Iron Oxide Nanoparticles on PC-3 and 4T1 Cancer Cell Lines

doi: 10.3390/pharmaceutics15010127

Figure Lengend Snippet: TEM images of the 4T1 cells after 2 h incubation with MNP-SS ( A – D ) and MNP-PEG ( E – H ): ( A )—cytoplasm of 4T1 cell with a vesicle filled with MNP-SS nanoparticles; the vesicle is enlarged in ( B , C )—outer membrane and cytoplasm of the 4T1 cell with attached clumps of MNP-SS nanoparticles and just formed vesicles; the vesicle is enlarged in ( D )—filled with nanoparticles; ( E )—4T1 cell plasmalemma with attached MNP-PEG nanoparticles, enlarged in ( F – H )—MNP-PEG nanoparticles in the vicinity of the 4T1 cell plasmalemma.

Article Snippet: PC-3 human prostate cancer cells and 4T1 mouse breast cancer cells (American Type Culture Collection, Manassas, VA, USA) were cultured in a 5% CO 2 atmosphere at 37 °C in DMEM/F12 medium containing 10% fetal bovine serum (Sigma-Aldrich, Burlington, VT, USA), 1% L-glutamine (Gibco, Waltham, MA, USA), and 1% antibiotics (penicillin and streptomycin).

Techniques: Incubation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Sushi domain-containing protein 4 binds to epithelial growth factor receptor and initiates autophagy in an EGFR phosphorylation independent manner

doi: 10.1186/s13046-022-02565-1

Figure Lengend Snippet: SUSD4 expression suppresses tumor growth and affect EGFR dynamics. Tumor growth monitored in a syngeneic mouse model. 4 T1-Luc2 cells stably expressing mouse SUSD4, or mock control cells were injected into the mammary fat pad of BALB/c mice ( n = 10 mice per group). In first experiment ( A ) 2 × 10 6 cells were injected per mouse and in the second ( B ) 5 × 10 6 cells were injected per mouse (Error bars represents standard error of the mean). ( C ) Migration and invasion assay ( D ) of 5 × 10 4 4 T1-Luc2 cells incubated for 48 h under serum. (E) Immunodetection of SUSD4 from lysates of BT-20 cells stably expressing human SUSD4 or mock control cells treated with deglycosylation enzymes to assess SUSD4 N-glycosylation status. (F) Protein profile comparison between SUSD4-expressing BT-20 cells and mock control cells using the Human XL Oncology Array ( n = 2 technical repeats). (G) Expression of SUSD4 and EGFR, independent of each other, in individual cell types present in breast cancer tumors. Representative immunoblots depicting total EGFR levels and phospho-EGFR at Tyr1045 (H) or Tyr1086 (I) in SUSD4-expressing BT-20 or MDA-MB-468 cells and corresponding mock control cells. Densitometric analysis of total EGFR and phospho-EGFR levels together with the ratio of phosphorylated EGFR to total EGFR in BT-20 cells (J) and MDA-MB-468 cells (K). (L) EGFR ubiquitination assessed by immunoprecipitation of EGFR in serum-starved EGF-treated BT-20 cell expressing SUSD4 or mock control cells followed by immunoblot detection of ubiquitin. All results were repeated in at least three independent biological experiments unless otherwise specified. The p -value was estimated with t test when two groups were compared and with Two-way ANOVA test when two or more groups were compared for two independent parameters * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001

Article Snippet: The 4 T1-Luc2 mouse breast cancer cells (ATCC) were harvested with Versene (Thermo Fisher Scientific), counted, washed two times in PBS, and resuspended in PBS.

Techniques: Expressing, Stable Transfection, Injection, Migration, Invasion Assay, Incubation, Immunodetection, Western Blot, Immunoprecipitation

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: “Derived Multiple Allogeneic Protein Paracrine Signaling (d-MAPPS)” Enhances T Cell-Driven Immune Response to Murine Mammary Carcinoma

doi: 10.1155/2022/3655595

Figure Lengend Snippet: d-MAPPS delayed mammary tumor appearance and inhibited tumor growth. (a) Mean value of time period in days from inoculation of 4T1 breast cancer cells to the appearance of palpable primary tumor in 4T1+saline-treated (black bars) and 4T1+d-MAPPS-treated BALB/c mice (white bars). (b) Percentage of 4T1+saline- and 4T1+d-MAPPS-treated BALB/c mice after 36 days of follow-up. (c) Light-microscopic pictures (magnification, ×10) through liver (upper panels), lung (middle panels), and brain tissue sections (lower panels) showing metastatic colonies (black arrows). (d) Survival of 4T1+saline-treated (blue line) and 4T1+d-MAPPS-treated mice (red line). The mean value of primary tumor (e) volume and (f) weight at day 36. Data are presented as mean + /−SEM ( n = 16 mice/group; ∗∗∗ p < 0.001).

Article Snippet: Mice from experimental groups received 5 × 10 4 mouse breast cancer 4T1 cells (purchased from American Type Culture Collection (ATCC, USA)) into the fourth mammary fat pad [ ].

Techniques:

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: “Derived Multiple Allogeneic Protein Paracrine Signaling (d-MAPPS)” Enhances T Cell-Driven Immune Response to Murine Mammary Carcinoma

doi: 10.1155/2022/3655595

Figure Lengend Snippet: d-MAPPS enhanced concentration of antitumorigenic and reduced concentration of immunosuppressive cytokines in serum and tissue samples of tumor-bearing mice. Concentration of antitumorigenic (CXCL16, IL-27, IFN- γ , and IL-17) and immunosuppressive cytokines (TGF- β and IL-10) in (a–f) serum and (g) tumor tissue samples of BALB/c mice from experimental groups (4T1+saline-treated (black bars) and 4T1+d-MAPPS-treated animals (white bars)) and control groups (saline-treated (dark grey bars) and d-MAPPS-treated animals (light grey bars)). Data are presented as mean + /−SEM ( n = 16 mice/experimental group and n = 8/control group; ∗∗∗ p < 0.001).

Article Snippet: Mice from experimental groups received 5 × 10 4 mouse breast cancer 4T1 cells (purchased from American Type Culture Collection (ATCC, USA)) into the fourth mammary fat pad [ ].

Techniques: Concentration Assay

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: “Derived Multiple Allogeneic Protein Paracrine Signaling (d-MAPPS)” Enhances T Cell-Driven Immune Response to Murine Mammary Carcinoma

doi: 10.1155/2022/3655595

Figure Lengend Snippet: d-MAPPS did not significantly alter phenotype and function of tumor-infiltrated NK cells and macrophages. Flow cytometry analysis and intracellular staining of (a) tumor-infiltrated CD178+, (b) granzyme B+, (c) IFN- γ +, IL-17+ NK1.1+NK cells and (e) IL-12+, (f) TNF- α +, (g) IL-10+, (h) CD80+, (i) CD86+, and (j) I-A+ F4/80+ macrophages in the tumors of 4T1+saline-treated (black bars) and 4T1+d-MAPPS-treated mice (white bars). Data are presented as mean + /−SEM ( n = 16 mice/group).

Article Snippet: Mice from experimental groups received 5 × 10 4 mouse breast cancer 4T1 cells (purchased from American Type Culture Collection (ATCC, USA)) into the fourth mammary fat pad [ ].

Techniques: Flow Cytometry, Staining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: “Derived Multiple Allogeneic Protein Paracrine Signaling (d-MAPPS)” Enhances T Cell-Driven Immune Response to Murine Mammary Carcinoma

doi: 10.1155/2022/3655595

Figure Lengend Snippet: d-MAPPS improved antigen-presenting properties of tumor-infiltrated dendritic cells. Flow cytometry analysis and intracellular staining of tumor-infiltrated (a) I-A-expressing TNF- α -producing CD11c+, (b) I-A-expressing CD11c+, (c) CD80- and CD86-expressing CD11c+, (d) IL-12-producing CD11c+, and (e) IL-10-producing CD11c+ DCs in the tumors of 4T1+saline-treated (black bars) and 4T1+d-MAPPS-treated mice (white bars). Data are presented as mean + /−SEM ( n = 16 mice/group; ∗∗∗ p < 0.001).

Article Snippet: Mice from experimental groups received 5 × 10 4 mouse breast cancer 4T1 cells (purchased from American Type Culture Collection (ATCC, USA)) into the fourth mammary fat pad [ ].

Techniques: Flow Cytometry, Staining, Expressing

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: “Derived Multiple Allogeneic Protein Paracrine Signaling (d-MAPPS)” Enhances T Cell-Driven Immune Response to Murine Mammary Carcinoma

doi: 10.1155/2022/3655595

Figure Lengend Snippet: d-MAPPS increased antitumorigenic CD4+ Th1 and Th17 cells and decreased immunosuppressive T regulatory cells in the tumors of 4T1-treated mice. Total number of (a) IFN- γ -producing CD4+ Th1 cells, (b) IL-17-producing CD4+ Th17 cells, (c) IL-4-producing CD4+ Th2 cells, (d) IL-10-producing CD4+ T cells, and (e) FoxP3-expressing CD4+ Tregs in the tumors of 4T1+saline-treated (black bars) and 4T1+d-MAPPS-treated mice (white bars), as determined by the flow cytometry analysis and intracellular staining of tumor-infiltrated mononuclear cells. Data are presented as mean + /−SEM ( n = 16 mice/group; ∗ p < 0.05; ∗∗∗ p < 0.001).

Article Snippet: Mice from experimental groups received 5 × 10 4 mouse breast cancer 4T1 cells (purchased from American Type Culture Collection (ATCC, USA)) into the fourth mammary fat pad [ ].

Techniques: Expressing, Flow Cytometry, Staining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: “Derived Multiple Allogeneic Protein Paracrine Signaling (d-MAPPS)” Enhances T Cell-Driven Immune Response to Murine Mammary Carcinoma

doi: 10.1155/2022/3655595

Figure Lengend Snippet: d-MAPPS increased number of tumoricidal CD8+ CTLs and decreased presence of immunosuppressive CD8+ T cells in the tumors of 4T1-treated mice. Total number of (a) tumorotoxic CD178+CD8+ CTLs, (b) granzyme B-expressing CD8+ CTLs, (c) IFN- γ -producing CD8+ Th1, and (d) IL-17-producing CD8+ Th17 cells and percentage of (e) immunosuppressive IL-10-producing CD8+ and (f) FoxP3-expressing CD8+ T regulatory cells in the tumors of 4T1+saline-treated (black bars) and 4T1+d-MAPPS-treated mice (white bars), as determined by the flow cytometry analysis and intracellular staining of tumor-infiltrated mononuclear cells. Data are presented as mean + /−SEM ( n = 16 mice/group; ∗ p < 0.05; ∗∗∗ p < 0.001).

Article Snippet: Mice from experimental groups received 5 × 10 4 mouse breast cancer 4T1 cells (purchased from American Type Culture Collection (ATCC, USA)) into the fourth mammary fat pad [ ].

Techniques: Expressing, Flow Cytometry, Staining