Journal: Scientific Reports

Article Title: Self-propelling, protein-bound magnetic nanobots for efficient in vitro drug delivery in triple negative breast cancer cells

doi: 10.1038/s41598-024-83393-5

Figure Lengend Snippet: Anticancer activity of Fe, Fe-GSH, Fe-GSH-Protein, Fe-GSH-Protein-Dox ( A ), Fe-GSH-Protein-Dox ( C ), and Dox by MTT assay. MDA-MB-231 and 4T1 cells were treated with Dox at a concentration range of 3.5 to 200 µg/mL ( n = 3). The IC 50 value of Fe, Fe-GSH, Fe-GSH-Protein, Fe-GSH-Protein-Dox ( A ), Fe-GSH-Protein-Dox ( C ), and Dox for cultured 4T1 ( A , B ) and MDA-MB-231 ( C , D ) cells at 24 h and 48 h.

Article Snippet: The human epithelial breast cancer cell line (MDA-MB-231) and mouse epithelial breast cancer cell line (4T1) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Activity Assay, MTT Assay, Concentration Assay, Cell Culture

Journal: Scientific Reports

Article Title: Self-propelling, protein-bound magnetic nanobots for efficient in vitro drug delivery in triple negative breast cancer cells

doi: 10.1038/s41598-024-83393-5

Figure Lengend Snippet: Cellular internalization study of free Dox and Fe-GSH-Protein-Dox ( A ) in 4T1 cells and the results were observed after 1 and 4 h of incubation (A).The histogram and a bar graph were used to demonstrate the amount of NPs taken up by 4T1 cell lines using flow cytometry, with a statistical significance of p < 0.01 ( B ).

Article Snippet: The human epithelial breast cancer cell line (MDA-MB-231) and mouse epithelial breast cancer cell line (4T1) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Incubation, Flow Cytometry

Journal: Scientific Reports

Article Title: Self-propelling, protein-bound magnetic nanobots for efficient in vitro drug delivery in triple negative breast cancer cells

doi: 10.1038/s41598-024-83393-5

Figure Lengend Snippet: Internalization mechanism of Fe-GSH-Protein-Dox ( A ). Cellular uptake of the Fe-GSH-Protein-Dox ( A ) was checked following treatment with endocytosis inhibitors. After 4 h treatment with Fe-GSH-Protein-Dox ( A ), MDA-MB-231 cells and 4T1 cells were observed for fluorescence using confocal microscopy ( A ) and ( B ). Fe-GSH-Protein-Dox (A)with Chlorpromazine, Fe-GSH-Protein-Dox ( A ) with Nystatin, and Fe-GSH-Protein-Dox ( A ) with Amiloride.

Article Snippet: The human epithelial breast cancer cell line (MDA-MB-231) and mouse epithelial breast cancer cell line (4T1) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Fluorescence, Confocal Microscopy

Journal: Scientific Reports

Article Title: Self-propelling, protein-bound magnetic nanobots for efficient in vitro drug delivery in triple negative breast cancer cells

doi: 10.1038/s41598-024-83393-5

Figure Lengend Snippet: Intracellular ROS generation in MDA-MB-231 and 4T1 cells treated with free Dox and Fe-GSH-Protein-Dox (A) by confocal microscopy ( A , C ) and Flow cytometry analysis of MDA-MB-231 and 4T1 cell lines ( B and D ).

Article Snippet: The human epithelial breast cancer cell line (MDA-MB-231) and mouse epithelial breast cancer cell line (4T1) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Confocal Microscopy, Flow Cytometry

Journal: Scientific Reports

Article Title: Self-propelling, protein-bound magnetic nanobots for efficient in vitro drug delivery in triple negative breast cancer cells

doi: 10.1038/s41598-024-83393-5

Figure Lengend Snippet: Cell cycle analysis. The arrest of cell cycle due to the treatment of Dox, Fe-GSH-Protein-Dox ( A ) on MDA-MB-231 and 4T1 cell lines was observed using flow cytometry ( A ) and ( B ).

Article Snippet: The human epithelial breast cancer cell line (MDA-MB-231) and mouse epithelial breast cancer cell line (4T1) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Cell Cycle Assay, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Indocyanine Blue (ICB) as a Functional Alternative to Indocyanine Green (ICG) for Enhanced 700 nm NIR Imaging

doi: 10.3390/ijms252413547

Figure Lengend Snippet: Tumor targeting of ICB in 4T1 breast tumor-bearing animal models. ( a ) Mice weighing 25 g were injected with 50 nmol of ICB, and their tumor signals were observed up to 48 h post-injection. ( b ) Simultaneous intraoperative dual-channel imaging of tumors and lymphatic vessels (LV; red arrows). A total of 7.5 nmol of ICB (red) was injected intratumorally (black arrows) 4 h post-injection of cRGD-ZW800-1 (green). Abbreviations used are In, intestine; LV, lymphatic vessel; Tu, tumor.

Article Snippet: The mouse breast cancer cell line 4T1 cells were purchased from ATCC (Manassas, VA, USA).

Techniques: Injection, Imaging