4t1 atcc crl 2539  (ATCC)

Journal: PLOS ONE

Article Title: A novel TREX1 inhibitor, VB-85680, upregulates cellular interferon responses

doi: 10.1371/journal.pone.0305962

Figure Lengend Snippet: A) TREX1 Western blot demonstrating overexpression of both wild-type mouse TREX1 and mouse TREX1 carrying the D18N inactivating mutation. Endogenous mouse TREX1 present in a J774 cellular lysate is shown as reference to confirm the size of the upper band as full-length mouse TREX1. α-tubulin was used as a loading control. B–D) Exonuclease assays performed with HEK293T mouse TREX1 overexpression or empty vector lysates demonstrate the effects of VB-85680 and VB-85662 on B) wild-type mouse TREX1 (5 ng/μL total protein), C) Catalytically inactive mouse TREX1 D18N (100 ng/μL total protein) and, D) endogenous exonuclease activity (100 ng/μL total protein). E) Western blot for endogenous levels of cGAS,STING and TREX1 in both 4T1 and THP1-dual cells. F) Endogenous exonuclease activity in 4T1 cell lysates is inhibited by VB-85680, but not by VB-85662. Compounds were titrated in the presence of 6 μg 4T1 cytosolic lysate using the 3’ to 5’ Exonuclease Activity Assay from BioVision. G) Inhibition of endogenous human TREX1 exonuclease activity by VB-85680 in THP1-Dual™ and THP1-Dual™ TREX1 knockout cell lysates. The exonuclease assay was carried out as described in 1E . Error bars for all experiments represent +/- SD.

Article Snippet: Briefly, THP1-Dual™ (Invivogen thpd-nfis), THP1-Dual™ TREX1 KO (Invivogen thpd-nfis) or 4T1 (ATCC CRL-2539) cells were suspended at a density of 1 million cells/mL in the lysis buffer provided.

Techniques: Western Blot, Over Expression, Mutagenesis, Control, Plasmid Preparation, Activity Assay, Inhibition, Knock-Out

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

doi: 10.3389/fbioe.2024.1430637

Figure Lengend Snippet: 4T1 (grey) and CHO-K1 (blue) cell membrane permeabilization to Yo-Pro1 fluorescent marker. Experiments were performed using No.1 EP buffer. μs PEF—1.2 kV/cm × 100 μs × 8, 1 Hz and nsPEF—2.5–10 kV/cm × 300 ns × 250, 1 MHz. Asterisk (*) corresponds to statistically significant ( p < 0.05) difference.

Article Snippet: Murine 4T1 (ATCC-CRL-2539), a mammary gland tumor cell line of BALB/c origin and Chinese Hamster Ovary CHO-K1 (ATCC-CCL-61) cell lines were grown in RPMI 1640 medium supplemented with glutamine, 100 U/mL of penicillin, 100 mg/mL of streptomycin, and 10% of fetal bovine serum (FBS).

Techniques: Membrane, Marker

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

doi: 10.3389/fbioe.2024.1430637

Figure Lengend Snippet: (A) CHO-K1 cell line electrotransfection efficiency dependence on EP buffers composition (No. 1, two and 5) with Luciferase-pcDNA3 plasmid, where µsPEF 1.2 kV/cm × 100 μs × 8, 1 Hz and nsPEF 5 kV/cm × 300 ns × 250, 1 MHz. (B) 4T1 cell line electrotransfection efficiency during µsPEF (1.2 kV/cm × 100 μs × 8, 1 Hz) and nsPEF (2.5–10 kV/cm × 300 ns × 250, 1 MHz), with Luciferase-pcDNA3 plasmid (EP buffer No.1). Asterisk (*) corresponds to statistically significant ( p < 0.05) difference.

Article Snippet: Murine 4T1 (ATCC-CRL-2539), a mammary gland tumor cell line of BALB/c origin and Chinese Hamster Ovary CHO-K1 (ATCC-CCL-61) cell lines were grown in RPMI 1640 medium supplemented with glutamine, 100 U/mL of penicillin, 100 mg/mL of streptomycin, and 10% of fetal bovine serum (FBS).

Techniques: Luciferase, Plasmid Preparation

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

doi: 10.3389/fbioe.2024.1430637

Figure Lengend Snippet: CHO-K1 (blue) and 4T1 (grey) normalized cell viability after electroporation with Luciferase-pcDNA3 plasmid. Both experiments were performed using No.1 EP buffer. Asterisk (*) corresponds to statistically significant ( p < 0.05) difference.

Article Snippet: Murine 4T1 (ATCC-CRL-2539), a mammary gland tumor cell line of BALB/c origin and Chinese Hamster Ovary CHO-K1 (ATCC-CCL-61) cell lines were grown in RPMI 1640 medium supplemented with glutamine, 100 U/mL of penicillin, 100 mg/mL of streptomycin, and 10% of fetal bovine serum (FBS).

Techniques: Electroporation, Luciferase, Plasmid Preparation

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses

doi: 10.3389/fbioe.2024.1430637

Figure Lengend Snippet: BALB/c murine imaging, where 4T1-luc clone was used for tumor induction. Images were taken at day 0 (when tumor volume reached 50 mm 3 ) and day 14. The color bar on the right side of the image illustrates the correlation between color and light intensity, measured in arbitrary units (counts), for the entire animal images. Images were taken with IVIS Spectrum device and analyzed by Living Image software.

Article Snippet: Murine 4T1 (ATCC-CRL-2539), a mammary gland tumor cell line of BALB/c origin and Chinese Hamster Ovary CHO-K1 (ATCC-CCL-61) cell lines were grown in RPMI 1640 medium supplemented with glutamine, 100 U/mL of penicillin, 100 mg/mL of streptomycin, and 10% of fetal bovine serum (FBS).

Techniques: Imaging, Software

Journal: British Journal of Cancer

Article Title: The in vitro dynamics of pseudo-vascular network formation

doi: 10.1038/s41416-024-02722-7

Figure Lengend Snippet: Growth metrics for each cell line compared to the literature.

Article Snippet: MDA-MB-231 (HTB-26™, ATCC, UK) and 4T1 (CRL-2539™, ATCC, UK) cell lines were used as mammary breast carcinomas of human and murine origin respectively.

Techniques:

Journal: British Journal of Cancer

Article Title: The in vitro dynamics of pseudo-vascular network formation

doi: 10.1038/s41416-024-02722-7

Figure Lengend Snippet: a Illustration of the meniscus effect within a cell culture well. b Binary masks of whole well with 4T1-T cells forming tubular structures for 3 representative timepoints. Notice that the cellularity of the periphery of the well is low for all timepoints shown. Scale bar is set to 1 mm. c For two morphological objects (number of isolated segments and pieces) for the 4T1-T cell line, average object value (y-axis) versus time (x-axis) is shown for each well (grey dots). Coloured lines correspond to the fit of the third-order polynomial linear model estimated by means of three estimators: the linear regression (dotted lines), the robust regression (dashed lines) and the quantile regression (solid line). Coloured points correspond to wells with outliers.

Article Snippet: MDA-MB-231 (HTB-26™, ATCC, UK) and 4T1 (CRL-2539™, ATCC, UK) cell lines were used as mammary breast carcinomas of human and murine origin respectively.

Techniques: Cell Culture, Isolation