Structured Review

GE Healthcare 4mb glutathione sepharose
4mb Glutathione Sepharose, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4mb glutathione sepharose/product/GE Healthcare
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
4mb glutathione sepharose - by Bioz Stars, 2020-09
99/100 stars

Related Products / Commonly Used Together

glutathione - s - transferase
src
sh3 domains
p85

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Related Articles

Transfection:

Article Title: HCMV Encoded Glycoprotein M (UL100) Interacts with Rab11 Effector Protein FIP4
Article Snippet: .. Two days post transfection cells were lysed divided for equal fractions and incubated with sepharose beads containing purified GST-gM-CT. After precipitation and extensive washing, proteins remaining on the glutathione sepharose beads were resolved by SDS-PAGE, transferred to membranes and probed with anti-HA, anti-myc antibody and developed with HRP. (1.1M, tif) .. This work was supported by grants from the HHS, NIH (AI49537, AI50189 to WJB).

Affinity Chromatography:

Article Title: BRCA1 interacts with components of the histone deacetylase complex
Article Snippet: .. GST fusion proteins were purified by affinity chromatography with glutathione-Sepharose (Amersham Pharmacia) beads as described , and protein concentration was estimated on a Coomassie-blue stained SDS/PAGE gel. .. Purified pGST-HD (huntingtin) was a gift of H. Reddy (National Human Genome Research Institute).

Isolation:

Article Title: TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production
Article Snippet: .. A mock isolation using recombinant GST bound to 50 µl glutathione sepharose (GE Healthcare) served as a control. .. After batch binding beads were washed twice with 750 mM KOAc, 0.5% Nonidet P40 and twice with PBS.

Purification:

Article Title: BRCA1 interacts with components of the histone deacetylase complex
Article Snippet: .. GST fusion proteins were purified by affinity chromatography with glutathione-Sepharose (Amersham Pharmacia) beads as described , and protein concentration was estimated on a Coomassie-blue stained SDS/PAGE gel. .. Purified pGST-HD (huntingtin) was a gift of H. Reddy (National Human Genome Research Institute).

Article Title: HCMV Encoded Glycoprotein M (UL100) Interacts with Rab11 Effector Protein FIP4
Article Snippet: .. Two days post transfection cells were lysed divided for equal fractions and incubated with sepharose beads containing purified GST-gM-CT. After precipitation and extensive washing, proteins remaining on the glutathione sepharose beads were resolved by SDS-PAGE, transferred to membranes and probed with anti-HA, anti-myc antibody and developed with HRP. (1.1M, tif) .. This work was supported by grants from the HHS, NIH (AI49537, AI50189 to WJB).

Article Title: TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction
Article Snippet: .. For the GST-LC3B[G120] affinity-isolation assay, HEK293 cells expressing TP53INP2[NLSΔ], TP53INP2[Δ1-28],[NLSΔ] TP53INP2W35,I38A [NLSΔ], TP53INP2[Δ67-111],[NLSΔ] or TP53INP2[Δ112-144],[NLSΔ] were lysed and the cell lysate was incubated with purified GST or GST-LC3B[G120] proteins at 4°C for 4 h. Then the glutathione-sepharose 4B beads were added to the mixture followed by incubation at 4°C for 2 h. Immunocomplexes were washed and used for western blot. .. Protein bands were detected with Coomassie Brilliant Blue and quantified using the ImageJ software.

Incubation:

Article Title: HCMV Encoded Glycoprotein M (UL100) Interacts with Rab11 Effector Protein FIP4
Article Snippet: .. Two days post transfection cells were lysed divided for equal fractions and incubated with sepharose beads containing purified GST-gM-CT. After precipitation and extensive washing, proteins remaining on the glutathione sepharose beads were resolved by SDS-PAGE, transferred to membranes and probed with anti-HA, anti-myc antibody and developed with HRP. (1.1M, tif) .. This work was supported by grants from the HHS, NIH (AI49537, AI50189 to WJB).

Article Title: The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes
Article Snippet: .. Then, GST and GST fusion proteins were incubated with glutathione-Sepharose 4B (Amersham Biosciences) for 2 h at 4 °C followed by extensively washing with PBS containing 1% Triton. .. The GST fusion proteins were then eluted by glutathione, and the purity of the proteins was examined by electrophoresis.

Article Title: Regulated protein degradation controls PKA function and cell-type differentiation in Dictyostelium
Article Snippet: .. Samples were centrifuged at 15,000 rpm for 10 min. We incubated the supernatant with 80 μL of GST beads (glutathione–Sepharose 4B, purchased from Amersham Pharmacia Biotech) for 1 h at 4°C with gentle rocking. .. The beads were washed with NP-40-containing lysis buffer three times and with 1× PBS twice, and the fusion protein was eluted from the beads by boiling in 1× SDS sample buffer.

Article Title: TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction
Article Snippet: .. For the GST-LC3B[G120] affinity-isolation assay, HEK293 cells expressing TP53INP2[NLSΔ], TP53INP2[Δ1-28],[NLSΔ] TP53INP2W35,I38A [NLSΔ], TP53INP2[Δ67-111],[NLSΔ] or TP53INP2[Δ112-144],[NLSΔ] were lysed and the cell lysate was incubated with purified GST or GST-LC3B[G120] proteins at 4°C for 4 h. Then the glutathione-sepharose 4B beads were added to the mixture followed by incubation at 4°C for 2 h. Immunocomplexes were washed and used for western blot. .. Protein bands were detected with Coomassie Brilliant Blue and quantified using the ImageJ software.

Article Title: The CRL4Cdt2 Ubiquitin Ligase Mediates the Proteolysis of Cyclin-Dependent Kinase Inhibitor Xic1 through a Direct Association with PCNA ▿
Article Snippet: .. For glutathione S -transferase (GST) pulldown assays, GST-Xic1, GST-hp21, and GST-hp27 fusion proteins (5 μg) were bound to glutathione-Sepharose 4B (GE Healthcare) and incubated with [35 S]methionine-labeled XCdt2 (4 μl) for 1.5 h at 23°C. .. For GST-XCdt21-400 , GST-XCdt2401-710 , and GST-XPCNA, beads were incubated with purified MBP-Xic1 or XPCNA (5, 15, 25, or 50 μg).

Expressing:

Article Title: TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction
Article Snippet: .. For the GST-LC3B[G120] affinity-isolation assay, HEK293 cells expressing TP53INP2[NLSΔ], TP53INP2[Δ1-28],[NLSΔ] TP53INP2W35,I38A [NLSΔ], TP53INP2[Δ67-111],[NLSΔ] or TP53INP2[Δ112-144],[NLSΔ] were lysed and the cell lysate was incubated with purified GST or GST-LC3B[G120] proteins at 4°C for 4 h. Then the glutathione-sepharose 4B beads were added to the mixture followed by incubation at 4°C for 2 h. Immunocomplexes were washed and used for western blot. .. Protein bands were detected with Coomassie Brilliant Blue and quantified using the ImageJ software.

Protein Concentration:

Article Title: BRCA1 interacts with components of the histone deacetylase complex
Article Snippet: .. GST fusion proteins were purified by affinity chromatography with glutathione-Sepharose (Amersham Pharmacia) beads as described , and protein concentration was estimated on a Coomassie-blue stained SDS/PAGE gel. .. Purified pGST-HD (huntingtin) was a gift of H. Reddy (National Human Genome Research Institute).

Western Blot:

Article Title: TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction
Article Snippet: .. For the GST-LC3B[G120] affinity-isolation assay, HEK293 cells expressing TP53INP2[NLSΔ], TP53INP2[Δ1-28],[NLSΔ] TP53INP2W35,I38A [NLSΔ], TP53INP2[Δ67-111],[NLSΔ] or TP53INP2[Δ112-144],[NLSΔ] were lysed and the cell lysate was incubated with purified GST or GST-LC3B[G120] proteins at 4°C for 4 h. Then the glutathione-sepharose 4B beads were added to the mixture followed by incubation at 4°C for 2 h. Immunocomplexes were washed and used for western blot. .. Protein bands were detected with Coomassie Brilliant Blue and quantified using the ImageJ software.

Recombinant:

Article Title: TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production
Article Snippet: .. A mock isolation using recombinant GST bound to 50 µl glutathione sepharose (GE Healthcare) served as a control. .. After batch binding beads were washed twice with 750 mM KOAc, 0.5% Nonidet P40 and twice with PBS.

SDS Page:

Article Title: BRCA1 interacts with components of the histone deacetylase complex
Article Snippet: .. GST fusion proteins were purified by affinity chromatography with glutathione-Sepharose (Amersham Pharmacia) beads as described , and protein concentration was estimated on a Coomassie-blue stained SDS/PAGE gel. .. Purified pGST-HD (huntingtin) was a gift of H. Reddy (National Human Genome Research Institute).

Article Title: HCMV Encoded Glycoprotein M (UL100) Interacts with Rab11 Effector Protein FIP4
Article Snippet: .. Two days post transfection cells were lysed divided for equal fractions and incubated with sepharose beads containing purified GST-gM-CT. After precipitation and extensive washing, proteins remaining on the glutathione sepharose beads were resolved by SDS-PAGE, transferred to membranes and probed with anti-HA, anti-myc antibody and developed with HRP. (1.1M, tif) .. This work was supported by grants from the HHS, NIH (AI49537, AI50189 to WJB).

Staining:

Article Title: BRCA1 interacts with components of the histone deacetylase complex
Article Snippet: .. GST fusion proteins were purified by affinity chromatography with glutathione-Sepharose (Amersham Pharmacia) beads as described , and protein concentration was estimated on a Coomassie-blue stained SDS/PAGE gel. .. Purified pGST-HD (huntingtin) was a gift of H. Reddy (National Human Genome Research Institute).