Journal: Journal of Textile Design Research and Practice

Article Title: Sensory Textile-Bacterial Hybrids: Textile-Bacteria Fusion to Impart Forest-Associated Scents

doi: 10.1080/20511787.2024.2394267

Figure Lengend Snippet: Figure 4 Streptomyces violaceruber bacterial growth curve showing mid-log and stationary growth phases.

Article Snippet: Sensory Textile-Bacterial Hybrids Jo ur na lo f Te xt ile D es ig n R es ea rc h an d P ra ct ic e 7 Cultivation of Bacterial Strain, Streptomyces Violaceruber [DSMZ 40783] Streptomyces violaceruber (DSMZ 40783), a soil microorganism was initially selected.

Techniques:

Journal: Metabolites

Article Title: Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers.

doi: 10.3390/metabo13030374

Figure Lengend Snippet: Figure 1. Quantitative profiles of extracellular metabolites reported as extracted ion chromatogram (EIC) area and identified in K. rhizophila, S. coelicolor, and S. violaceoruber cultivations, in S. coelicolor and S. violaceoruber (double) co-cultures, and in S. coelicolor, S. violaceoruber, and K. rhizophila (triple) co-cultures. The values are reported as the mean of three cultivations; SE values are also reported as bars. Asterisks indicate quantitative group differences that are statistically significant (p < 0.05) according to the one-way ANOVA test.

Article Snippet: Streptomyces coelicolor M145 and Kocuria rhizophila were from our laboratory collection; Streptomyces violaceoruber DSM 40783 was purchased from DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).

Techniques:

Journal: Metabolites

Article Title: Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers.

doi: 10.3390/metabo13030374

Figure Lengend Snippet: Figure 2. Tomato shoot (A) and root (B) responses to the PGPB treatments after 12 DAT analyzed with one-way ANOVA followed by Tukey’s post hoc test (p < 0.05). Different letters indicate sta- tistically significant differences among treatments in pairwise comparisons. Ct: control; VIOL: S. violaceoruber; M145: S. coelicolor; KOC: K. rhizophila; VIOL + KOC: S. violaceoruber and K. rhi- zophila mixes; M145 + KOC: S. coelicolor and K. rhizophila mixture; VIOL + M145: S. violaceoruber and S. coelicolor mixture.

Article Snippet: Streptomyces coelicolor M145 and Kocuria rhizophila were from our laboratory collection; Streptomyces violaceoruber DSM 40783 was purchased from DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).

Techniques: Control

Journal: Cellular Microbiology

Article Title: Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection

doi: 10.1111/cmi.12587

Figure Lengend Snippet: Relative expression of inflammatory mediators genes: TNFA (A), IL6 (B), IL1B (C), IL12A (D), IL2 (E) and TGFBRII (F) mRNA in patients with chronic gastritis H. pylori ‐negative (Hp−) and H. pylori ‐positive before (Hp+) and after eradication treatment (ER) groups. Data are presented as RQ values, and each point represents one individual. Line symbolizes RQ median for each group. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.

Article Snippet: Initially, sections were incubated with specific primary antibodies at 4 °C overnight: rabbit polyclonal TNF alpha antibody (NB600‐587, 1:200 dilution; Novus Biologicals ), rabbit polyclonal IL1 beta antibody (NB600‐633, 1:200 dilution; Novus Biologicals ), rabbit polyclonal IL6 antibody (NB600‐1131, 1:400 dilution; Novus Biologicals ), rabbit monoclonal IL2 antibody (NBP1‐40687, 1:250 dilution; Novus Biologicals ), rabbit polyclonal TGF beta Receptor II antibody (NBP1‐19434, 1:100 dilution; Novus Biologicals ) and rabbit monoclonal IL‐12 alpha antibody (TA310616, 1:500 dilution; OriGene ).

Techniques: Expressing

Journal: Cellular Microbiology

Article Title: Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection

doi: 10.1111/cmi.12587

Figure Lengend Snippet: Immunoexpression of the proteins IL‐12p40, IL‐2 and TGF‐β‐RII, considering negative (absent and weak) and positive (moderate and strong): normal gastric mucosa (A, B, C, respectively), chronic gastritis not infected with H. pylori (D, E, F, respectively) and chronic gastritis Hp+ before (G, H, I, respectively) and after eradication treatment (J, K, L, respectively). In normal mucosa, IL‐12p40 (A), IL‐2 (B) and TGF‐β‐RII (C) presented mainly weak immunostaining in the cytoplasm of foveolar epithelium (arrow). The Hp− group presented predominance of weak or absent immunostaining in the epithelium (arrow) to IL‐12p40 (D) and IL‐2 (E) and strong to TGF‐β‐RII (F). For IL‐12p40 and IL‐2, Hp+ group (G and H, respectively) showed strong immunostaining in the cytoplasm of foveolar epithelium (arrow) and inflammatory cells (arrowhead) with a reduction in the IL‐2 expression in epithelium (K) and reduction in immunostaining intensity for IL‐12‐p40 after eradication (J). For TGF‐β‐RII, Hp+ group (I) presented weak immunostaining in the cytoplasm of foveolar epithelium and strong in inflammatory cells (arrowhead) with reduction after eradication only in the inflammatory cells (arrowhead) (L). Counterstain: Haematoxylin. Bars: 50 µm. Quantitative image analysis (M‐O) showed the cytoplasm immunostaining intensity (median ± interquartile range). a.u. arbitrary unit; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.

Article Snippet: Initially, sections were incubated with specific primary antibodies at 4 °C overnight: rabbit polyclonal TNF alpha antibody (NB600‐587, 1:200 dilution; Novus Biologicals ), rabbit polyclonal IL1 beta antibody (NB600‐633, 1:200 dilution; Novus Biologicals ), rabbit polyclonal IL6 antibody (NB600‐1131, 1:400 dilution; Novus Biologicals ), rabbit monoclonal IL2 antibody (NBP1‐40687, 1:250 dilution; Novus Biologicals ), rabbit polyclonal TGF beta Receptor II antibody (NBP1‐19434, 1:100 dilution; Novus Biologicals ) and rabbit monoclonal IL‐12 alpha antibody (TA310616, 1:500 dilution; OriGene ).

Techniques: Infection, Immunostaining, Expressing

Journal: Cellular Microbiology

Article Title: Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection

doi: 10.1111/cmi.12587

Figure Lengend Snippet: Protein interaction network showing miRNAs and their predicted gene targets. The protein interaction network (red lines) shows the interaction between proteins encoded by target genes that are predicted to be regulated by miRNAs. Predicted interactions between miRNAs and target genes are shown by the continuous gray lines. Dotted gray line represents some interaction possibilities obtained by experimental validation in this study between, for example, miR‐103 and the gene IL12A and between mir‐223 and genes IL2 , TNFA and IL1B. Ellipses represent target genes/proteins; blue triangles represent miRNAs.

Article Snippet: Initially, sections were incubated with specific primary antibodies at 4 °C overnight: rabbit polyclonal TNF alpha antibody (NB600‐587, 1:200 dilution; Novus Biologicals ), rabbit polyclonal IL1 beta antibody (NB600‐633, 1:200 dilution; Novus Biologicals ), rabbit polyclonal IL6 antibody (NB600‐1131, 1:400 dilution; Novus Biologicals ), rabbit monoclonal IL2 antibody (NBP1‐40687, 1:250 dilution; Novus Biologicals ), rabbit polyclonal TGF beta Receptor II antibody (NBP1‐19434, 1:100 dilution; Novus Biologicals ) and rabbit monoclonal IL‐12 alpha antibody (TA310616, 1:500 dilution; OriGene ).

Techniques: Biomarker Discovery