hcov  (Sino Biological)


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    Name:
    Human coronavirus Spike Protein
    Description:
    A DNA sequence encoding the human coronavirus HCoV NL63 spike protein S1 S2 ECD APF29071 1 Met1 Pro1296 was expressed with a polyhistidine tag at the C terminus
    Catalog Number:
    40604-V08B
    Price:
    None
    Category:
    recombinant protein
    Host:
    Baculovirus-Insect Cells
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    Structured Review

    Sino Biological hcov
    A DNA sequence encoding the human coronavirus HCoV NL63 spike protein S1 S2 ECD APF29071 1 Met1 Pro1296 was expressed with a polyhistidine tag at the C terminus
    https://www.bioz.com/result/hcov/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov - by Bioz Stars, 2021-06
    95/100 stars

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    Related Articles

    Purification:

    Article Title: Cross-reactive antibody against human coronavirus OC43 spike protein correlates with disease severity in COVID-19 patients: a retrospective study
    Article Snippet: Plain enzyme-linked immunosorbent assay (ELISA)We developed plain ELISA protocols for detecting IgG against S proteins of seasonal HCoVs and SARS-CoV-2 as reported elsewhere, which was used to detect IgG against S protein of SARS-CoV-2 [ ]. .. The purified ectodomain of S proteins of HCoV-NL63 (Met1–Pro1296), -229E (Cys16–Trp1115), -OC43 (Met1–Pro1304), -HKU1 (Met1–Pro1295), and SARS-CoV-2 (Val 16–Pro1213), which were expressed in insect cells (Sino Biological, Beijing, China), were used as coating antigens (20 ng/well), respectively. .. Horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Sigma-Aldrich, St. Louis, MO, USA) diluted as 1:60,000 was used as the secondary antibody.

    Recombinant:

    Article Title: Direct activation of the alternative complement pathway by SARS-CoV-2 spike proteins is blocked by factor D inhibition
    Article Snippet: Human CoV proteins Recombinant proteins expressed with the Escherichia coli system include SARS-CoV-2 S1 subunit protein (receptor-binding domain [RBD]) (S1; RayBiotech), S2 subunit (S2; RayBiotech), and nucleocapsid protein (N; ABclonal Technology). .. Recombinant proteins expressed with the baculovirus-insect cell system include human CoV spike protein (HCoV-OC43 S; Sino Biological). ..

    other:

    Article Title: Small-Molecule In Vitro Inhibitors of the Coronavirus Spike – ACE2 Protein-Protein Interaction as Blockers of Viral Attachment and Entry for SARS-CoV-2
    Article Snippet: SARS-CoV bound with about similar potency as SARS-CoV-2 (13.9 nM; 1843 ng/mL), whereas HCoV-NL63 had significantly lower affinity (45.8 nM, 3610 ng/mL) ( ).

    Immunofluorescence:

    Article Title: High prevalence of pre-existing serological cross-reactivity against SARS-CoV-2 in sub-Sahara Africa
    Article Snippet: .. Immunofluorescence assay against SARS-CoV-2 and other human coronaviruses To detect the presence of serological cross-reactivity against SARS-CoV-2 and other human coronaviruses, we used an immunofluorescence assay (IFA) against the spike and nucleocapsid proteins of SARS, SARS-CoV-2, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E. .. Briefly, HEK-293 T cells (ATCC, USA) were transfected with mammalian expression plasmids encoding either the spike or nucleocapsid proteins of the respective coronaviruses (Addgene and Sino Biological, USA).

    Staining:

    Article Title: Interferon-β and mycophenolic acid are potent inhibitors of Middle East respiratory syndrome coronavirus in cell-based assays
    Article Snippet: .. MERS-CoV was detected with a rabbit polyclonal antibody to the HCoV-EMC/2012 S protein (Sino Biological) followed by staining with Alexa Fluor 594-conjugated goat anti-rabbit IgG (H+L) antibody (Life Technologies). ..

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    Sino Biological hcov nl63
    Concentration-response curves for binding of CoV spike protein domains to human ACE2 in cell-free ELISA-type assays. Binding curves and corresponding EC 50 s are shown for SARS-CoV-2 (RBD and S1), SARS-CoV (S1 S2), and <t>HCoV-NL63</t> (S1). They were obtained using Fc-conjugated hACE2 coated on the plate and His-tagged S1, S1S2, or RBD added in increasing amounts as shown with the amount bound detected using an anti-His–HRP conjugate (mean ± SD for two experiments in duplicates).
    Hcov Nl63, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov nl63/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov nl63 - by Bioz Stars, 2021-06
    95/100 stars
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    97
    Sino Biological human coronavirus spike protein
    Epitope-specificities and cross-reactivity of SARS-CoV-2 antibodies. The percentage of mAbs from each donor specific for the SARS-CoV-2 <t>spike</t> subdomains and their cross-reactivity was determined by BLI. (A) mAbs were grouped into the antibodies that bound RBD in the S1 subunit (S1: RBD, blue), mAbs that bound S1 outside of RBD (S1: non-RBD, teal), mAbs that bound the S2 ECD (S2 ECD, yellow) or those that bound S2P but did not bind either S1 or S2 (S2P: Non-S1/Non-S2. (B) The percentage of mAbs that bind to SARS-CoV-1, MERS and the four common <t>human</t> <t>coronavirus</t> was also measured by BLI. Significant differences were determined using two-way ANOVA with Tukey’s multiple comparison test, *p
    Human Coronavirus Spike Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human coronavirus spike protein/product/Sino Biological
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronavirus spike protein - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

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    Concentration-response curves for binding of CoV spike protein domains to human ACE2 in cell-free ELISA-type assays. Binding curves and corresponding EC 50 s are shown for SARS-CoV-2 (RBD and S1), SARS-CoV (S1 S2), and HCoV-NL63 (S1). They were obtained using Fc-conjugated hACE2 coated on the plate and His-tagged S1, S1S2, or RBD added in increasing amounts as shown with the amount bound detected using an anti-His–HRP conjugate (mean ± SD for two experiments in duplicates).

    Journal: bioRxiv

    Article Title: Small-Molecule In Vitro Inhibitors of the Coronavirus Spike – ACE2 Protein-Protein Interaction as Blockers of Viral Attachment and Entry for SARS-CoV-2

    doi: 10.1101/2020.10.22.351056

    Figure Lengend Snippet: Concentration-response curves for binding of CoV spike protein domains to human ACE2 in cell-free ELISA-type assays. Binding curves and corresponding EC 50 s are shown for SARS-CoV-2 (RBD and S1), SARS-CoV (S1 S2), and HCoV-NL63 (S1). They were obtained using Fc-conjugated hACE2 coated on the plate and His-tagged S1, S1S2, or RBD added in increasing amounts as shown with the amount bound detected using an anti-His–HRP conjugate (mean ± SD for two experiments in duplicates).

    Article Snippet: SARS-CoV bound with about similar potency as SARS-CoV-2 (13.9 nM; 1843 ng/mL), whereas HCoV-NL63 had significantly lower affinity (45.8 nM, 3610 ng/mL) ( ).

    Techniques: Concentration Assay, Binding Assay, Enzyme-linked Immunosorbent Assay

    mAb epitope mapping and cross-reactivity BLI. Related to Figure 3. The binding of the 198 SARS-CoV-2-specific mAbs was assessed by BLI for epitope and cross-reactivity. (A, B) All spike-specific mAbs isolated from COVID+ donors were tested by BLI for binding to SARS-CoV-2 S2P (A) and SARS-CoV-2 RBD (B). (C-H) The number of amino acid mutations (sum of heavy chain and light chain mutations) for each for each SARS-CoV-2 mAb that bound (blue dots) or didn’t bind (teal dots) SARS-COV-1 S-2P (C), SARS-COV-1 RBD (D) , MERS S-2P (E) , OC43 (F) , HKU1 (G) , NL63 (H) , or 229E (EI) . Statistics were assessed by Mann-Whitney test, no comparisons were significant.

    Journal: bioRxiv

    Article Title: Isolation and Characterization of Cross-Neutralizing Coronavirus Antibodies from COVID-19+ Subjects

    doi: 10.1101/2021.03.23.436684

    Figure Lengend Snippet: mAb epitope mapping and cross-reactivity BLI. Related to Figure 3. The binding of the 198 SARS-CoV-2-specific mAbs was assessed by BLI for epitope and cross-reactivity. (A, B) All spike-specific mAbs isolated from COVID+ donors were tested by BLI for binding to SARS-CoV-2 S2P (A) and SARS-CoV-2 RBD (B). (C-H) The number of amino acid mutations (sum of heavy chain and light chain mutations) for each for each SARS-CoV-2 mAb that bound (blue dots) or didn’t bind (teal dots) SARS-COV-1 S-2P (C), SARS-COV-1 RBD (D) , MERS S-2P (E) , OC43 (F) , HKU1 (G) , NL63 (H) , or 229E (EI) . Statistics were assessed by Mann-Whitney test, no comparisons were significant.

    Article Snippet: HCoV-OC43, HCoV-HKU1, HCoV-NL63 and HCoV-229E S1+S2 ECTs (Cat#’s 40607-V08B, 40606-V08B, 40604-V08B, 40605-V08D), SARS-HCoV-2 S1 domain (CAT#: 40591-V08B1), SARS-CoV-2 S1 N-terminal domain (CAT#40591-V41H) SARS-HCoV-2 S2 extra-cellular domain (CAT#: 40590-V08B) and SARS-CoV2 RBD (CAT#: 40150-V05H) were purchased from SinoBiologicals.

    Techniques: Binding Assay, Isolation, MANN-WHITNEY

    SARS-CoV-2 spike proteins induce complement-mediated cell killing that can be blocked by complement inhibitors in the mHam assay. TF1 PIGA null cells were treated with 20% NHS preincubated with diluted SARS-CoV-2 spike protein subunit 1 (S1), subunit 2 (S2), N proteins (N), and HCoV-OC43 S proteins (2.5 µg/mL to 20 µg/mL), and then measured for cell killing. Complement-mediated cell killing (%) was markedly increased in a dose-dependent manner with addition of S1 (A) and S2 (B). Increasing the concentration of N protein (C) and HCoV-OC43 S protein (D) did not increase the cell death (%) from baseline NHS level. Complement inhibition with 1 µM factor D inhibitor or 50 µg of anti-C5 monoclonal antibody completely blocked the cell killing induced by 20 µg/mL S1 (E) and S2 (F). The dotted line at 20% nonviable cells was established as a threshold for a positive mHam based on a receiver operative curve. All experiments were repeated at least 3 times. Statistical significance was calculated between each CoV protein–treated group and the NHS-treated group (* P

    Journal: Blood

    Article Title: Direct activation of the alternative complement pathway by SARS-CoV-2 spike proteins is blocked by factor D inhibition

    doi: 10.1182/blood.2020008248

    Figure Lengend Snippet: SARS-CoV-2 spike proteins induce complement-mediated cell killing that can be blocked by complement inhibitors in the mHam assay. TF1 PIGA null cells were treated with 20% NHS preincubated with diluted SARS-CoV-2 spike protein subunit 1 (S1), subunit 2 (S2), N proteins (N), and HCoV-OC43 S proteins (2.5 µg/mL to 20 µg/mL), and then measured for cell killing. Complement-mediated cell killing (%) was markedly increased in a dose-dependent manner with addition of S1 (A) and S2 (B). Increasing the concentration of N protein (C) and HCoV-OC43 S protein (D) did not increase the cell death (%) from baseline NHS level. Complement inhibition with 1 µM factor D inhibitor or 50 µg of anti-C5 monoclonal antibody completely blocked the cell killing induced by 20 µg/mL S1 (E) and S2 (F). The dotted line at 20% nonviable cells was established as a threshold for a positive mHam based on a receiver operative curve. All experiments were repeated at least 3 times. Statistical significance was calculated between each CoV protein–treated group and the NHS-treated group (* P

    Article Snippet: Recombinant proteins expressed with the baculovirus-insect cell system include human CoV spike protein (HCoV-OC43 S; Sino Biological).

    Techniques: Concentration Assay, Inhibition

    SARS-CoV-2 spike proteins induce C5b-9 deposition on the cell surface mainly through the alternative pathway. Flow cytometry demonstrated C5b-9 deposition on TF1 PIGA null cells after adding NHS preincubated with SARS-CoV-2 S1, S2, N, and HCoV-OC43 S proteins (2.5 µg/mL to 20 µg/mL) in either all pathway buffer (GVB ++ pH 7.4) or APC-specific buffer (GVB 0 10 mM MgEGTA pH 6.4). (A) SARS-CoV-2 S1, S2, and HCoV-OC43 S proteins elevated C5b-9 deposition in a dose-dependent manner in GVB ++ buffer, whereas N protein did not increase C5b-9 from the baseline NHS level. (B) Both SARS-CoV-2 S1 and S2 led to marked increase of C5b-9 depositions in APC-specific buffer. By contrast, SARS-CoV-2 N and HCoV-OC43 S proteins showed minimal C5b-9 increase in APC-specific buffer. All experiments were repeated 6 times. Statistical significance was calculated between each 20 µg/mL CoV protein-treated group and the NHS-treated group (* P

    Journal: Blood

    Article Title: Direct activation of the alternative complement pathway by SARS-CoV-2 spike proteins is blocked by factor D inhibition

    doi: 10.1182/blood.2020008248

    Figure Lengend Snippet: SARS-CoV-2 spike proteins induce C5b-9 deposition on the cell surface mainly through the alternative pathway. Flow cytometry demonstrated C5b-9 deposition on TF1 PIGA null cells after adding NHS preincubated with SARS-CoV-2 S1, S2, N, and HCoV-OC43 S proteins (2.5 µg/mL to 20 µg/mL) in either all pathway buffer (GVB ++ pH 7.4) or APC-specific buffer (GVB 0 10 mM MgEGTA pH 6.4). (A) SARS-CoV-2 S1, S2, and HCoV-OC43 S proteins elevated C5b-9 deposition in a dose-dependent manner in GVB ++ buffer, whereas N protein did not increase C5b-9 from the baseline NHS level. (B) Both SARS-CoV-2 S1 and S2 led to marked increase of C5b-9 depositions in APC-specific buffer. By contrast, SARS-CoV-2 N and HCoV-OC43 S proteins showed minimal C5b-9 increase in APC-specific buffer. All experiments were repeated 6 times. Statistical significance was calculated between each 20 µg/mL CoV protein-treated group and the NHS-treated group (* P

    Article Snippet: Recombinant proteins expressed with the baculovirus-insect cell system include human CoV spike protein (HCoV-OC43 S; Sino Biological).

    Techniques: Flow Cytometry

    Epitope-specificities and cross-reactivity of SARS-CoV-2 antibodies. The percentage of mAbs from each donor specific for the SARS-CoV-2 spike subdomains and their cross-reactivity was determined by BLI. (A) mAbs were grouped into the antibodies that bound RBD in the S1 subunit (S1: RBD, blue), mAbs that bound S1 outside of RBD (S1: non-RBD, teal), mAbs that bound the S2 ECD (S2 ECD, yellow) or those that bound S2P but did not bind either S1 or S2 (S2P: Non-S1/Non-S2. (B) The percentage of mAbs that bind to SARS-CoV-1, MERS and the four common human coronavirus was also measured by BLI. Significant differences were determined using two-way ANOVA with Tukey’s multiple comparison test, *p

    Journal: bioRxiv

    Article Title: Isolation and Characterization of Cross-Neutralizing Coronavirus Antibodies from COVID-19+ Subjects

    doi: 10.1101/2021.03.23.436684

    Figure Lengend Snippet: Epitope-specificities and cross-reactivity of SARS-CoV-2 antibodies. The percentage of mAbs from each donor specific for the SARS-CoV-2 spike subdomains and their cross-reactivity was determined by BLI. (A) mAbs were grouped into the antibodies that bound RBD in the S1 subunit (S1: RBD, blue), mAbs that bound S1 outside of RBD (S1: non-RBD, teal), mAbs that bound the S2 ECD (S2 ECD, yellow) or those that bound S2P but did not bind either S1 or S2 (S2P: Non-S1/Non-S2. (B) The percentage of mAbs that bind to SARS-CoV-1, MERS and the four common human coronavirus was also measured by BLI. Significant differences were determined using two-way ANOVA with Tukey’s multiple comparison test, *p

    Article Snippet: HCoV-OC43, HCoV-HKU1, HCoV-NL63 and HCoV-229E S1+S2 ECTs (Cat#’s 40607-V08B, 40606-V08B, 40604-V08B, 40605-V08D), SARS-HCoV-2 S1 domain (CAT#: 40591-V08B1), SARS-CoV-2 S1 N-terminal domain (CAT#40591-V41H) SARS-HCoV-2 S2 extra-cellular domain (CAT#: 40590-V08B) and SARS-CoV2 RBD (CAT#: 40150-V05H) were purchased from SinoBiologicals.

    Techniques: