human coronaviruses  (Sino Biological)


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    Name:
    Human coronavirus HCoV 229E Spike S1 Protein S1 Subunit His Tag
    Description:
    A DNA sequence encoding the human coronavirus HCoV 229E spike protein S1 Subunit APT69883 1 Cys16 Asn536 was expressed with a polyhistidine tag at the C terminus
    Catalog Number:
    40601-V08H
    Price:
    None
    Category:
    recombinant protein
    Host:
    HEK293 Cells
    Buy from Supplier


    Structured Review

    Sino Biological human coronaviruses
    Immunofluorescence assay (IFA) against SARS, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E spike or nucleocapsid expressing cells. Representative pictures of IFA with pre-COVID-19 pandemic cross-reactive plasma samples 21854. Sample 21854 strongly recognized the spike protein of HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E, but not SARS and MERS. Sample 21854 only recognized the nucleocapsid of HCoV-NL63 and not the other human <t>coronaviruses.</t> White arrows indicate positive cells. Scale bar represent 50 µm.
    A DNA sequence encoding the human coronavirus HCoV 229E spike protein S1 Subunit APT69883 1 Cys16 Asn536 was expressed with a polyhistidine tag at the C terminus
    https://www.bioz.com/result/human coronaviruses/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronaviruses - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "High prevalence of pre-existing serological cross-reactivity against SARS-CoV-2 in sub-Sahara Africa"

    Article Title: High prevalence of pre-existing serological cross-reactivity against SARS-CoV-2 in sub-Sahara Africa

    Journal: International Journal of Infectious Diseases

    doi: 10.1016/j.ijid.2020.10.104

    Immunofluorescence assay (IFA) against SARS, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E spike or nucleocapsid expressing cells. Representative pictures of IFA with pre-COVID-19 pandemic cross-reactive plasma samples 21854. Sample 21854 strongly recognized the spike protein of HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E, but not SARS and MERS. Sample 21854 only recognized the nucleocapsid of HCoV-NL63 and not the other human coronaviruses. White arrows indicate positive cells. Scale bar represent 50 µm.
    Figure Legend Snippet: Immunofluorescence assay (IFA) against SARS, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E spike or nucleocapsid expressing cells. Representative pictures of IFA with pre-COVID-19 pandemic cross-reactive plasma samples 21854. Sample 21854 strongly recognized the spike protein of HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E, but not SARS and MERS. Sample 21854 only recognized the nucleocapsid of HCoV-NL63 and not the other human coronaviruses. White arrows indicate positive cells. Scale bar represent 50 µm.

    Techniques Used: Immunofluorescence, Expressing

    Related Articles

    Immunofluorescence:

    Article Title: High prevalence of pre-existing serological cross-reactivity against SARS-CoV-2 in sub-Sahara Africa
    Article Snippet: .. Immunofluorescence assay against SARS-CoV-2 and other human coronaviruses To detect the presence of serological cross-reactivity against SARS-CoV-2 and other human coronaviruses, we used an immunofluorescence assay (IFA) against the spike and nucleocapsid proteins of SARS, SARS-CoV-2, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E. .. Briefly, HEK-293 T cells (ATCC, USA) were transfected with mammalian expression plasmids encoding either the spike or nucleocapsid proteins of the respective coronaviruses (Addgene and Sino Biological, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Immune Responses to SARS-CoV-2 Infection in Hospitalized Pediatric and Adult Patients
    Article Snippet: .. ELISA Assay Recombinant SARS Cov-2 spike protein (ACROBiosystems #S1N-C52H3), HKU1 S1 protein (Sino Biological #40021-V08H-100), 229E S1 protein (Sino Biological # 40601-V08H-100) or NL63 S1 protein (Sino Biological # 40600-V08H-100) at a concentration of 100 ng in 50μl PBS) were added to 96-well MaxiSorp plates (Thermo Scientific #442404) and incubated overnight at 4°C. .. Plates were washed three times with 250 μL ELISA wash buffer PBS-T (PBS with 0.1% Tween-20) and incubated with 200 μl of blocking solution (PBS with 0.1% Tween-20, 3% milk powder) at room temperature (RT) for 1.5 hours.

    Recombinant:

    Article Title: Immune Responses to SARS-CoV-2 Infection in Hospitalized Pediatric and Adult Patients
    Article Snippet: .. ELISA Assay Recombinant SARS Cov-2 spike protein (ACROBiosystems #S1N-C52H3), HKU1 S1 protein (Sino Biological #40021-V08H-100), 229E S1 protein (Sino Biological # 40601-V08H-100) or NL63 S1 protein (Sino Biological # 40600-V08H-100) at a concentration of 100 ng in 50μl PBS) were added to 96-well MaxiSorp plates (Thermo Scientific #442404) and incubated overnight at 4°C. .. Plates were washed three times with 250 μL ELISA wash buffer PBS-T (PBS with 0.1% Tween-20) and incubated with 200 μl of blocking solution (PBS with 0.1% Tween-20, 3% milk powder) at room temperature (RT) for 1.5 hours.

    Concentration Assay:

    Article Title: Immune Responses to SARS-CoV-2 Infection in Hospitalized Pediatric and Adult Patients
    Article Snippet: .. ELISA Assay Recombinant SARS Cov-2 spike protein (ACROBiosystems #S1N-C52H3), HKU1 S1 protein (Sino Biological #40021-V08H-100), 229E S1 protein (Sino Biological # 40601-V08H-100) or NL63 S1 protein (Sino Biological # 40600-V08H-100) at a concentration of 100 ng in 50μl PBS) were added to 96-well MaxiSorp plates (Thermo Scientific #442404) and incubated overnight at 4°C. .. Plates were washed three times with 250 μL ELISA wash buffer PBS-T (PBS with 0.1% Tween-20) and incubated with 200 μl of blocking solution (PBS with 0.1% Tween-20, 3% milk powder) at room temperature (RT) for 1.5 hours.

    Incubation:

    Article Title: Immune Responses to SARS-CoV-2 Infection in Hospitalized Pediatric and Adult Patients
    Article Snippet: .. ELISA Assay Recombinant SARS Cov-2 spike protein (ACROBiosystems #S1N-C52H3), HKU1 S1 protein (Sino Biological #40021-V08H-100), 229E S1 protein (Sino Biological # 40601-V08H-100) or NL63 S1 protein (Sino Biological # 40600-V08H-100) at a concentration of 100 ng in 50μl PBS) were added to 96-well MaxiSorp plates (Thermo Scientific #442404) and incubated overnight at 4°C. .. Plates were washed three times with 250 μL ELISA wash buffer PBS-T (PBS with 0.1% Tween-20) and incubated with 200 μl of blocking solution (PBS with 0.1% Tween-20, 3% milk powder) at room temperature (RT) for 1.5 hours.

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  • 93
    Sino Biological human coronaviruses
    Immunofluorescence assay (IFA) against SARS, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E spike or nucleocapsid expressing cells. Representative pictures of IFA with pre-COVID-19 pandemic cross-reactive plasma samples 21854. Sample 21854 strongly recognized the spike protein of HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E, but not SARS and MERS. Sample 21854 only recognized the nucleocapsid of HCoV-NL63 and not the other human <t>coronaviruses.</t> White arrows indicate positive cells. Scale bar represent 50 µm.
    Human Coronaviruses, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human coronaviruses/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronaviruses - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    86
    Sino Biological human coronavirus spike s1 protein
    Immunofluorescence assay (IFA) against SARS, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E spike or nucleocapsid expressing cells. Representative pictures of IFA with pre-COVID-19 pandemic cross-reactive plasma samples 21854. Sample 21854 strongly recognized the spike protein of HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E, but not SARS and MERS. Sample 21854 only recognized the nucleocapsid of HCoV-NL63 and not the other human <t>coronaviruses.</t> White arrows indicate positive cells. Scale bar represent 50 µm.
    Human Coronavirus Spike S1 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human coronavirus spike s1 protein/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronavirus spike s1 protein - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Immunofluorescence assay (IFA) against SARS, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E spike or nucleocapsid expressing cells. Representative pictures of IFA with pre-COVID-19 pandemic cross-reactive plasma samples 21854. Sample 21854 strongly recognized the spike protein of HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E, but not SARS and MERS. Sample 21854 only recognized the nucleocapsid of HCoV-NL63 and not the other human coronaviruses. White arrows indicate positive cells. Scale bar represent 50 µm.

    Journal: International Journal of Infectious Diseases

    Article Title: High prevalence of pre-existing serological cross-reactivity against SARS-CoV-2 in sub-Sahara Africa

    doi: 10.1016/j.ijid.2020.10.104

    Figure Lengend Snippet: Immunofluorescence assay (IFA) against SARS, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E spike or nucleocapsid expressing cells. Representative pictures of IFA with pre-COVID-19 pandemic cross-reactive plasma samples 21854. Sample 21854 strongly recognized the spike protein of HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E, but not SARS and MERS. Sample 21854 only recognized the nucleocapsid of HCoV-NL63 and not the other human coronaviruses. White arrows indicate positive cells. Scale bar represent 50 µm.

    Article Snippet: Immunofluorescence assay against SARS-CoV-2 and other human coronaviruses To detect the presence of serological cross-reactivity against SARS-CoV-2 and other human coronaviruses, we used an immunofluorescence assay (IFA) against the spike and nucleocapsid proteins of SARS, SARS-CoV-2, MERS, HCoV-OC43, HCoV-HKU-1, HCoV-NL63 and HCoV-229E.

    Techniques: Immunofluorescence, Expressing