rbd  (Sino Biological)


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    Name:
    SARS CoV 2 2019 nCoV Spike RBD Gene ORF cDNA clone expression plasmid C Myc tag Codon Optimized COVID 19 Spike S RBD Research
    Description:
    Full length Clone DNA of SARS CoV 2 2019 nCoV Spike RBD
    Catalog Number:
    VG40592-CM
    Price:
    195.0
    Category:
    cDNA Clone
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Product Aliases:
    coronavirus spike cDNA ORF Clone 2019-nCoV, cov spike cDNA ORF Clone 2019-nCoV, ncov RBD cDNA ORF Clone 2019-nCoV, ncov s1 cDNA ORF Clone 2019-nCoV, ncov s2 cDNA ORF Clone 2019-nCoV, ncov spike cDNA ORF Clone 2019-nCoV, NCP-CoV RBD cDNA ORF Clone 2019-nCoV, NCP-CoV s1 cDNA ORF Clone 2019-nCoV, NCP-CoV s2 cDNA ORF Clone 2019-nCoV, NCP-CoV Spike cDNA ORF Clone 2019-nCoV, novel coronavirus RBD cDNA ORF Clone 2019-nCoV, novel coronavirus s1 cDNA ORF Clone 2019-nCoV, novel coronavirus s2 cDNA ORF Clone 2019-nCoV, novel coronavirus spike cDNA ORF Clone 2019-nCoV, RBD cDNA ORF Clone 2019-nCoV, S1 cDNA ORF Clone 2019-nCoV, S2 cDNA ORF Clone 2019-nCoV, Spike RBD cDNA ORF Clone 2019-nCoV
    Molecule Name:
    Spike
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    Structured Review

    Sino Biological rbd
    SARS CoV 2 2019 nCoV Spike RBD Gene ORF cDNA clone expression plasmid C Myc tag Codon Optimized COVID 19 Spike S RBD Research
    Full length Clone DNA of SARS CoV 2 2019 nCoV Spike RBD
    https://www.bioz.com/result/rbd/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rbd - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes"

    Article Title: A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes

    Journal: Nature Communications

    doi: 10.1038/s41467-021-22926-2

    Structural analysis of P4A1 Fab and SARS-CoV-2 RBD complex. a The overall P4A1-Fab-RBD complex structure superimposed with the hACE2-RBD complex. The P4A1 heavy chain (colored slate blue), light chain (colored salmon red), and hACE (colored pale green) are displayed in cartoon representation. The SARS-CoV-2 RBD is colored in gray and displayed in surface representation. b The epitope of P4A1 shown in surface representation. The CDR loops of heavy chain (HCDR) and light chain (LCDR) are colored in purple and magenta, respectively. The epitopes from the heavy chain and light chain are colored in slate blue and salmon red, respectively. The only residue K417, which contacts with both heavy chain and light chain, is colored in pink. The light-chain frame region 3 (LFR3) is colored in orange. The identical residues on RBD shared in P4A1 and hACE2 binding are labeled in red. The residues are numbered according to SARS-CoV-2 RBD. c The detailed interactions between SARS-CoV-2 RBD with HCDR, LCDR, and LFR3. The residues are shown in sticks with identical colors to ( b ).
    Figure Legend Snippet: Structural analysis of P4A1 Fab and SARS-CoV-2 RBD complex. a The overall P4A1-Fab-RBD complex structure superimposed with the hACE2-RBD complex. The P4A1 heavy chain (colored slate blue), light chain (colored salmon red), and hACE (colored pale green) are displayed in cartoon representation. The SARS-CoV-2 RBD is colored in gray and displayed in surface representation. b The epitope of P4A1 shown in surface representation. The CDR loops of heavy chain (HCDR) and light chain (LCDR) are colored in purple and magenta, respectively. The epitopes from the heavy chain and light chain are colored in slate blue and salmon red, respectively. The only residue K417, which contacts with both heavy chain and light chain, is colored in pink. The light-chain frame region 3 (LFR3) is colored in orange. The identical residues on RBD shared in P4A1 and hACE2 binding are labeled in red. The residues are numbered according to SARS-CoV-2 RBD. c The detailed interactions between SARS-CoV-2 RBD with HCDR, LCDR, and LFR3. The residues are shown in sticks with identical colors to ( b ).

    Techniques Used: Binding Assay, Labeling

    Related Articles

    Injection:

    Article Title: A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes
    Article Snippet: The antibody was immobilized onto a protein A sensor chip (GE Healthcare, Catalog #29139131-AA). .. Serially diluted SARS-CoV-2 RBD (WT, AcroBiosystems, Catalog #SPD-C52H3), RBD (V367F, AcroBiosystems, Catalog #SPD-S52H4), RBD (N354D/D364Y, AcroBiosystems, Catalog #SPD-S52H3), RBD (R408I, AcroBiosystems, Catalog #SPD-S52H8), RBD (W436R, AcroBiosystems, Catalog #SPD-S52H7), RBD (N439K, Sino Biological, Catalog #40592-V08H14), RBD (Y453F, Sino Biological, Catalog #40592-V08H80), RBD (S477N, Sino Biological, Catalog #40592-V08H46), RBD (F490S, Sino Biological, Catalog #40592-V08H41), RBD (S494P, Sino Biological, Catalog #40592-V08H18), RBD (N501Y, Sino Biological, Catalog #40592-V08H82), or SARS-CoV-2 spike S1 domain (D614G, Sino Biological, Catalog #40591-V08H3) were injected through flow cells for 60 or 180 s of association followed by a 150 or 800 s dissociation phase at a flow rate of 30 μL min−1 . ..

    Expressing:

    Article Title: Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without
    Article Snippet: Examination of sera from convalescent Covid-19 patients revealed potent neutralizing capacity against particle-cell fusion, but only moderate or low activity against cell-cell fusion. .. Expression plasmidsAn expression plasmid for codon-optimized SARS-CoV-2 S with a C-terminal HA tag (pCG-SARS-CoV-2-S-HA) was kindly provided by Karl-Klaus Conzelmann ( ).The Plasmid pCG-SARS-CoV-2-SΔ19 encoding the SΔ19 variant was generated by PCR amplification of the truncated S sequence, inserting PacI and SpeI restriction sites as well as a stop codon by PCR with primers 5’-TTATTAATTAAATGTTCGTGTTTCTGGTG-3’ and 5’-TATACTAGTTCTAGCAGCAGCTGCC-3’. .. The PCR fragment was inserted into the pCG backbone by restriction cloning.

    Plasmid Preparation:

    Article Title: Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without
    Article Snippet: Examination of sera from convalescent Covid-19 patients revealed potent neutralizing capacity against particle-cell fusion, but only moderate or low activity against cell-cell fusion. .. Expression plasmidsAn expression plasmid for codon-optimized SARS-CoV-2 S with a C-terminal HA tag (pCG-SARS-CoV-2-S-HA) was kindly provided by Karl-Klaus Conzelmann ( ).The Plasmid pCG-SARS-CoV-2-SΔ19 encoding the SΔ19 variant was generated by PCR amplification of the truncated S sequence, inserting PacI and SpeI restriction sites as well as a stop codon by PCR with primers 5’-TTATTAATTAAATGTTCGTGTTTCTGGTG-3’ and 5’-TATACTAGTTCTAGCAGCAGCTGCC-3’. .. The PCR fragment was inserted into the pCG backbone by restriction cloning.

    Variant Assay:

    Article Title: Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without
    Article Snippet: Examination of sera from convalescent Covid-19 patients revealed potent neutralizing capacity against particle-cell fusion, but only moderate or low activity against cell-cell fusion. .. Expression plasmidsAn expression plasmid for codon-optimized SARS-CoV-2 S with a C-terminal HA tag (pCG-SARS-CoV-2-S-HA) was kindly provided by Karl-Klaus Conzelmann ( ).The Plasmid pCG-SARS-CoV-2-SΔ19 encoding the SΔ19 variant was generated by PCR amplification of the truncated S sequence, inserting PacI and SpeI restriction sites as well as a stop codon by PCR with primers 5’-TTATTAATTAAATGTTCGTGTTTCTGGTG-3’ and 5’-TATACTAGTTCTAGCAGCAGCTGCC-3’. .. The PCR fragment was inserted into the pCG backbone by restriction cloning.

    Generated:

    Article Title: Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without
    Article Snippet: Examination of sera from convalescent Covid-19 patients revealed potent neutralizing capacity against particle-cell fusion, but only moderate or low activity against cell-cell fusion. .. Expression plasmidsAn expression plasmid for codon-optimized SARS-CoV-2 S with a C-terminal HA tag (pCG-SARS-CoV-2-S-HA) was kindly provided by Karl-Klaus Conzelmann ( ).The Plasmid pCG-SARS-CoV-2-SΔ19 encoding the SΔ19 variant was generated by PCR amplification of the truncated S sequence, inserting PacI and SpeI restriction sites as well as a stop codon by PCR with primers 5’-TTATTAATTAAATGTTCGTGTTTCTGGTG-3’ and 5’-TATACTAGTTCTAGCAGCAGCTGCC-3’. .. The PCR fragment was inserted into the pCG backbone by restriction cloning.

    Polymerase Chain Reaction:

    Article Title: Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without
    Article Snippet: Examination of sera from convalescent Covid-19 patients revealed potent neutralizing capacity against particle-cell fusion, but only moderate or low activity against cell-cell fusion. .. Expression plasmidsAn expression plasmid for codon-optimized SARS-CoV-2 S with a C-terminal HA tag (pCG-SARS-CoV-2-S-HA) was kindly provided by Karl-Klaus Conzelmann ( ).The Plasmid pCG-SARS-CoV-2-SΔ19 encoding the SΔ19 variant was generated by PCR amplification of the truncated S sequence, inserting PacI and SpeI restriction sites as well as a stop codon by PCR with primers 5’-TTATTAATTAAATGTTCGTGTTTCTGGTG-3’ and 5’-TATACTAGTTCTAGCAGCAGCTGCC-3’. .. The PCR fragment was inserted into the pCG backbone by restriction cloning.

    Amplification:

    Article Title: Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without
    Article Snippet: Examination of sera from convalescent Covid-19 patients revealed potent neutralizing capacity against particle-cell fusion, but only moderate or low activity against cell-cell fusion. .. Expression plasmidsAn expression plasmid for codon-optimized SARS-CoV-2 S with a C-terminal HA tag (pCG-SARS-CoV-2-S-HA) was kindly provided by Karl-Klaus Conzelmann ( ).The Plasmid pCG-SARS-CoV-2-SΔ19 encoding the SΔ19 variant was generated by PCR amplification of the truncated S sequence, inserting PacI and SpeI restriction sites as well as a stop codon by PCR with primers 5’-TTATTAATTAAATGTTCGTGTTTCTGGTG-3’ and 5’-TATACTAGTTCTAGCAGCAGCTGCC-3’. .. The PCR fragment was inserted into the pCG backbone by restriction cloning.

    Sequencing:

    Article Title: Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without
    Article Snippet: Examination of sera from convalescent Covid-19 patients revealed potent neutralizing capacity against particle-cell fusion, but only moderate or low activity against cell-cell fusion. .. Expression plasmidsAn expression plasmid for codon-optimized SARS-CoV-2 S with a C-terminal HA tag (pCG-SARS-CoV-2-S-HA) was kindly provided by Karl-Klaus Conzelmann ( ).The Plasmid pCG-SARS-CoV-2-SΔ19 encoding the SΔ19 variant was generated by PCR amplification of the truncated S sequence, inserting PacI and SpeI restriction sites as well as a stop codon by PCR with primers 5’-TTATTAATTAAATGTTCGTGTTTCTGGTG-3’ and 5’-TATACTAGTTCTAGCAGCAGCTGCC-3’. .. The PCR fragment was inserted into the pCG backbone by restriction cloning.

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  • About
  • News
  • Press Release
  • Team
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  • Bioz Stars
  • Bioz vStars
  • 94
    Sino Biological rbd
    Structural analysis of P4A1 Fab and <t>SARS-CoV-2</t> <t>RBD</t> complex. a The overall P4A1-Fab-RBD complex structure superimposed with the hACE2-RBD complex. The P4A1 heavy chain (colored slate blue), light chain (colored salmon red), and hACE (colored pale green) are displayed in cartoon representation. The SARS-CoV-2 RBD is colored in gray and displayed in surface representation. b The epitope of P4A1 shown in surface representation. The CDR loops of heavy chain (HCDR) and light chain (LCDR) are colored in purple and magenta, respectively. The epitopes from the heavy chain and light chain are colored in slate blue and salmon red, respectively. The only residue K417, which contacts with both heavy chain and light chain, is colored in pink. The light-chain frame region 3 (LFR3) is colored in orange. The identical residues on RBD shared in P4A1 and hACE2 binding are labeled in red. The residues are numbered according to SARS-CoV-2 RBD. c The detailed interactions between SARS-CoV-2 RBD with HCDR, LCDR, and LFR3. The residues are shown in sticks with identical colors to ( b ).
    Rbd, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rbd/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rbd - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    86
    Sino Biological mink variant
    Structural analysis of P4A1 Fab and <t>SARS-CoV-2</t> <t>RBD</t> complex. a The overall P4A1-Fab-RBD complex structure superimposed with the hACE2-RBD complex. The P4A1 heavy chain (colored slate blue), light chain (colored salmon red), and hACE (colored pale green) are displayed in cartoon representation. The SARS-CoV-2 RBD is colored in gray and displayed in surface representation. b The epitope of P4A1 shown in surface representation. The CDR loops of heavy chain (HCDR) and light chain (LCDR) are colored in purple and magenta, respectively. The epitopes from the heavy chain and light chain are colored in slate blue and salmon red, respectively. The only residue K417, which contacts with both heavy chain and light chain, is colored in pink. The light-chain frame region 3 (LFR3) is colored in orange. The identical residues on RBD shared in P4A1 and hACE2 binding are labeled in red. The residues are numbered according to SARS-CoV-2 RBD. c The detailed interactions between SARS-CoV-2 RBD with HCDR, LCDR, and LFR3. The residues are shown in sticks with identical colors to ( b ).
    Mink Variant, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mink variant/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mink variant - by Bioz Stars, 2021-06
    86/100 stars
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    Image Search Results


    Structural analysis of P4A1 Fab and SARS-CoV-2 RBD complex. a The overall P4A1-Fab-RBD complex structure superimposed with the hACE2-RBD complex. The P4A1 heavy chain (colored slate blue), light chain (colored salmon red), and hACE (colored pale green) are displayed in cartoon representation. The SARS-CoV-2 RBD is colored in gray and displayed in surface representation. b The epitope of P4A1 shown in surface representation. The CDR loops of heavy chain (HCDR) and light chain (LCDR) are colored in purple and magenta, respectively. The epitopes from the heavy chain and light chain are colored in slate blue and salmon red, respectively. The only residue K417, which contacts with both heavy chain and light chain, is colored in pink. The light-chain frame region 3 (LFR3) is colored in orange. The identical residues on RBD shared in P4A1 and hACE2 binding are labeled in red. The residues are numbered according to SARS-CoV-2 RBD. c The detailed interactions between SARS-CoV-2 RBD with HCDR, LCDR, and LFR3. The residues are shown in sticks with identical colors to ( b ).

    Journal: Nature Communications

    Article Title: A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes

    doi: 10.1038/s41467-021-22926-2

    Figure Lengend Snippet: Structural analysis of P4A1 Fab and SARS-CoV-2 RBD complex. a The overall P4A1-Fab-RBD complex structure superimposed with the hACE2-RBD complex. The P4A1 heavy chain (colored slate blue), light chain (colored salmon red), and hACE (colored pale green) are displayed in cartoon representation. The SARS-CoV-2 RBD is colored in gray and displayed in surface representation. b The epitope of P4A1 shown in surface representation. The CDR loops of heavy chain (HCDR) and light chain (LCDR) are colored in purple and magenta, respectively. The epitopes from the heavy chain and light chain are colored in slate blue and salmon red, respectively. The only residue K417, which contacts with both heavy chain and light chain, is colored in pink. The light-chain frame region 3 (LFR3) is colored in orange. The identical residues on RBD shared in P4A1 and hACE2 binding are labeled in red. The residues are numbered according to SARS-CoV-2 RBD. c The detailed interactions between SARS-CoV-2 RBD with HCDR, LCDR, and LFR3. The residues are shown in sticks with identical colors to ( b ).

    Article Snippet: Serially diluted SARS-CoV-2 RBD (WT, AcroBiosystems, Catalog #SPD-C52H3), RBD (V367F, AcroBiosystems, Catalog #SPD-S52H4), RBD (N354D/D364Y, AcroBiosystems, Catalog #SPD-S52H3), RBD (R408I, AcroBiosystems, Catalog #SPD-S52H8), RBD (W436R, AcroBiosystems, Catalog #SPD-S52H7), RBD (N439K, Sino Biological, Catalog #40592-V08H14), RBD (Y453F, Sino Biological, Catalog #40592-V08H80), RBD (S477N, Sino Biological, Catalog #40592-V08H46), RBD (F490S, Sino Biological, Catalog #40592-V08H41), RBD (S494P, Sino Biological, Catalog #40592-V08H18), RBD (N501Y, Sino Biological, Catalog #40592-V08H82), or SARS-CoV-2 spike S1 domain (D614G, Sino Biological, Catalog #40591-V08H3) were injected through flow cells for 60 or 180 s of association followed by a 150 or 800 s dissociation phase at a flow rate of 30 μL min−1 .

    Techniques: Binding Assay, Labeling