cov 2 spike rbd  (Sino Biological)


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    Name:
    SARS CoV 2 2019 nCoV Spike RBD His V483A Recombinant Protein COVID 19 Spike RBD Research
    Description:
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 V483A Arg319 Phe541 was expressed with a polyhistidine tag at the C terminus
    Catalog Number:
    40592-v08h5
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus spike Protein 2019-nCoV, cov spike Protein 2019-nCoV, ncov RBD Protein 2019-nCoV, ncov s1 Protein 2019-nCoV, ncov s2 Protein 2019-nCoV, ncov spike Protein 2019-nCoV, NCP-CoV RBD Protein 2019-nCoV, NCP-CoV s1 Protein 2019-nCoV, NCP-CoV s2 Protein 2019-nCoV, NCP-CoV Spike Protein 2019-nCoV, novel coronavirus RBD Protein 2019-nCoV, novel coronavirus s1 Protein 2019-nCoV, novel coronavirus s2 Protein 2019-nCoV, novel coronavirus spike Protein 2019-nCoV, RBD Protein 2019-nCoV, S1 Protein 2019-nCoV, S2 Protein 2019-nCoV, Spike RBD Protein 2019-nCoV
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological cov 2 spike rbd
    Large-scale mutagenesis of ACE2’s peptidase domain. (a) Analysis of how amino acid substitutions across positions 18-615 affect binding of the <t>SARS-CoV-2</t> spike protein. The plot quantifies the importance of each site by taking the mean of the absolute value of all mutation effects observed at that site. The grey line represents the mean absolute value of the mutation effect and the blue line shows the moving average to highlight general regions of ACE2 that are important for binding. Key structural landmarks are highlighted with shaded regions along the length of the sequence. (b) Mutation effect heat maps for three different regions of ACE2. Red denotes mutations that increase ACE2 spike binding; blue denotes reduced binding. Overall, we observe the effects of 3571 amino acid substitutions across 597 positions in ACE2’s peptidase domain. (c) The mean absolute mutation effect mapped onto the three-dimensional ACE2 structure (PDB ID: 6LZG). Residues near the spike interface are important for binding, in addition to many sites located on the distal lobe of the protein domain. (d) The most important region of ACE2 structure is composed of a tightly packed cluster of residues located over 30 Å from the spike interface.
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 V483A Arg319 Phe541 was expressed with a polyhistidine tag at the C terminus
    https://www.bioz.com/result/cov 2 spike rbd/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cov 2 spike rbd - by Bioz Stars, 2021-03
    94/100 stars

    Images

    1) Product Images from "Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein"

    Article Title: Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein

    Journal: bioRxiv

    doi: 10.1101/2020.09.17.301861

    Large-scale mutagenesis of ACE2’s peptidase domain. (a) Analysis of how amino acid substitutions across positions 18-615 affect binding of the SARS-CoV-2 spike protein. The plot quantifies the importance of each site by taking the mean of the absolute value of all mutation effects observed at that site. The grey line represents the mean absolute value of the mutation effect and the blue line shows the moving average to highlight general regions of ACE2 that are important for binding. Key structural landmarks are highlighted with shaded regions along the length of the sequence. (b) Mutation effect heat maps for three different regions of ACE2. Red denotes mutations that increase ACE2 spike binding; blue denotes reduced binding. Overall, we observe the effects of 3571 amino acid substitutions across 597 positions in ACE2’s peptidase domain. (c) The mean absolute mutation effect mapped onto the three-dimensional ACE2 structure (PDB ID: 6LZG). Residues near the spike interface are important for binding, in addition to many sites located on the distal lobe of the protein domain. (d) The most important region of ACE2 structure is composed of a tightly packed cluster of residues located over 30 Å from the spike interface.
    Figure Legend Snippet: Large-scale mutagenesis of ACE2’s peptidase domain. (a) Analysis of how amino acid substitutions across positions 18-615 affect binding of the SARS-CoV-2 spike protein. The plot quantifies the importance of each site by taking the mean of the absolute value of all mutation effects observed at that site. The grey line represents the mean absolute value of the mutation effect and the blue line shows the moving average to highlight general regions of ACE2 that are important for binding. Key structural landmarks are highlighted with shaded regions along the length of the sequence. (b) Mutation effect heat maps for three different regions of ACE2. Red denotes mutations that increase ACE2 spike binding; blue denotes reduced binding. Overall, we observe the effects of 3571 amino acid substitutions across 597 positions in ACE2’s peptidase domain. (c) The mean absolute mutation effect mapped onto the three-dimensional ACE2 structure (PDB ID: 6LZG). Residues near the spike interface are important for binding, in addition to many sites located on the distal lobe of the protein domain. (d) The most important region of ACE2 structure is composed of a tightly packed cluster of residues located over 30 Å from the spike interface.

    Techniques Used: Mutagenesis, Binding Assay, Sequencing

    Related Articles

    Mutagenesis:

    Article Title: Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein
    Article Snippet: Yeast cells were subsequently washed and resuspended in ice cold PBS for sorting on a FACS Aria III (Becton Dickinson, Franklin Lakes, NJ) located in the University of Wisconsin-Madison Carbone Cancer Center Flow Cytometry Laboratory. .. Sorting gates were set such that ACE2 mutant library members at or above approximately the 80th percentile with respect to binding to the CoV-2 spike RBD were isolated (Supplementary Figures 2 & 3). .. Yeast cells isolated during sorting were cultured overnight in low pH SDCAA media at 30°C with shaking at 250 rpm and the following morning 1/10th of the culture volume was expanded in low pH SDCAA to an OD of 0.1, shaken at 30°C and 250 rpm, and harvested by centrifugation after the OD had reached 0.4.

    Binding Assay:

    Article Title: Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein
    Article Snippet: Yeast cells were subsequently washed and resuspended in ice cold PBS for sorting on a FACS Aria III (Becton Dickinson, Franklin Lakes, NJ) located in the University of Wisconsin-Madison Carbone Cancer Center Flow Cytometry Laboratory. .. Sorting gates were set such that ACE2 mutant library members at or above approximately the 80th percentile with respect to binding to the CoV-2 spike RBD were isolated (Supplementary Figures 2 & 3). .. Yeast cells isolated during sorting were cultured overnight in low pH SDCAA media at 30°C with shaking at 250 rpm and the following morning 1/10th of the culture volume was expanded in low pH SDCAA to an OD of 0.1, shaken at 30°C and 250 rpm, and harvested by centrifugation after the OD had reached 0.4.

    Article Title: Methylene Blue Inhibits the SARS-CoV-2 Spike–ACE2 Protein-Protein Interaction–a Mechanism that can Contribute to its Antiviral Activity Against COVID-19
    Article Snippet: This was followed by blocking with 200 μL/well of SuperBlock (PBS) (Thermo Fisher Scientific) for 1 h at room temperature. .. Then, plates were washed twice using washing solution (PBS pH 7.4, 0.05% Tween-20) and tapped dry before the addition of the tagged ligand (SARS-CoV-2 S1 or RBD) and test compounds diluted in binding buffer (100 mM HEPES, pH 7.2) to give a total volume of 100 μL/well. .. After 1 h incubation, three washes were conducted, and a further 1 h incubation with anti-His HRP conjugate (BioLegend; San Diego, CA, Unites States; catalog no. 652504) diluted (1:2,500) in SuperBlock (PBS) was used to detect the bound His-tagged ligand.

    Article Title: Methylene Blue Inhibits In Vitro the SARS-CoV-2 Spike – ACE2 Protein-Protein Interaction – A Mechanism That Can Contribute to Its Antiviral Activity Against COVID-19
    Article Snippet: This was followed by blocking with 200 μL/well of SuperBlock (PBS) (Thermo Fisher Scientific) for 1 h at RT. .. Then, plates were washed twice using washing solution (PBS pH 7.4, 0.05% Tween-20) and tapped dry before the addition of the tagged ligand (SARS-CoV-2 S1 or RBD) and test compounds diluted in binding buffer (100 mM HEPES, pH 7.2) to give a total volume of 100 μL/well. .. After 1 h incubation, three washes were conducted, and a further 1 h incubation with anti-His HRP conjugate diluted (1:2500) in SuperBlock (PBS) was used to detect the bound His-tagged ligand.

    Isolation:

    Article Title: Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein
    Article Snippet: Yeast cells were subsequently washed and resuspended in ice cold PBS for sorting on a FACS Aria III (Becton Dickinson, Franklin Lakes, NJ) located in the University of Wisconsin-Madison Carbone Cancer Center Flow Cytometry Laboratory. .. Sorting gates were set such that ACE2 mutant library members at or above approximately the 80th percentile with respect to binding to the CoV-2 spike RBD were isolated (Supplementary Figures 2 & 3). .. Yeast cells isolated during sorting were cultured overnight in low pH SDCAA media at 30°C with shaking at 250 rpm and the following morning 1/10th of the culture volume was expanded in low pH SDCAA to an OD of 0.1, shaken at 30°C and 250 rpm, and harvested by centrifugation after the OD had reached 0.4.

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    • cov 2 spike rbd  (Sino Biological)
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    Sino Biological cov 2 spike rbd
    Large-scale mutagenesis of ACE2’s peptidase domain. (a) Analysis of how amino acid substitutions across positions 18-615 affect binding of the <t>SARS-CoV-2</t> spike protein. The plot quantifies the importance of each site by taking the mean of the absolute value of all mutation effects observed at that site. The grey line represents the mean absolute value of the mutation effect and the blue line shows the moving average to highlight general regions of ACE2 that are important for binding. Key structural landmarks are highlighted with shaded regions along the length of the sequence. (b) Mutation effect heat maps for three different regions of ACE2. Red denotes mutations that increase ACE2 spike binding; blue denotes reduced binding. Overall, we observe the effects of 3571 amino acid substitutions across 597 positions in ACE2’s peptidase domain. (c) The mean absolute mutation effect mapped onto the three-dimensional ACE2 structure (PDB ID: 6LZG). Residues near the spike interface are important for binding, in addition to many sites located on the distal lobe of the protein domain. (d) The most important region of ACE2 structure is composed of a tightly packed cluster of residues located over 30 Å from the spike interface.
    Cov 2 Spike Rbd, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cov 2 spike rbd/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cov 2 spike rbd - by Bioz Stars, 2021-03
    94/100 stars
    		  Buy from Supplier

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    Large-scale mutagenesis of ACE2’s peptidase domain. (a) Analysis of how amino acid substitutions across positions 18-615 affect binding of the SARS-CoV-2 spike protein. The plot quantifies the importance of each site by taking the mean of the absolute value of all mutation effects observed at that site. The grey line represents the mean absolute value of the mutation effect and the blue line shows the moving average to highlight general regions of ACE2 that are important for binding. Key structural landmarks are highlighted with shaded regions along the length of the sequence. (b) Mutation effect heat maps for three different regions of ACE2. Red denotes mutations that increase ACE2 spike binding; blue denotes reduced binding. Overall, we observe the effects of 3571 amino acid substitutions across 597 positions in ACE2’s peptidase domain. (c) The mean absolute mutation effect mapped onto the three-dimensional ACE2 structure (PDB ID: 6LZG). Residues near the spike interface are important for binding, in addition to many sites located on the distal lobe of the protein domain. (d) The most important region of ACE2 structure is composed of a tightly packed cluster of residues located over 30 Å from the spike interface.

    Journal: bioRxiv

    Article Title: Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein

    doi: 10.1101/2020.09.17.301861

    Figure Lengend Snippet: Large-scale mutagenesis of ACE2’s peptidase domain. (a) Analysis of how amino acid substitutions across positions 18-615 affect binding of the SARS-CoV-2 spike protein. The plot quantifies the importance of each site by taking the mean of the absolute value of all mutation effects observed at that site. The grey line represents the mean absolute value of the mutation effect and the blue line shows the moving average to highlight general regions of ACE2 that are important for binding. Key structural landmarks are highlighted with shaded regions along the length of the sequence. (b) Mutation effect heat maps for three different regions of ACE2. Red denotes mutations that increase ACE2 spike binding; blue denotes reduced binding. Overall, we observe the effects of 3571 amino acid substitutions across 597 positions in ACE2’s peptidase domain. (c) The mean absolute mutation effect mapped onto the three-dimensional ACE2 structure (PDB ID: 6LZG). Residues near the spike interface are important for binding, in addition to many sites located on the distal lobe of the protein domain. (d) The most important region of ACE2 structure is composed of a tightly packed cluster of residues located over 30 Å from the spike interface.

    Article Snippet: Sorting gates were set such that ACE2 mutant library members at or above approximately the 80th percentile with respect to binding to the CoV-2 spike RBD were isolated (Supplementary Figures 2 & 3).

    Techniques: Mutagenesis, Binding Assay, Sequencing