sars cov 2 rbd protein  (Sino Biological)


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    Name:
    SARS CoV 2 2019 nCoV Spike RBD A522V His Recombinant Protein
    Description:
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike RBD A522V His Recombinant Protein YP 009724390 1 Arg319 Phe541 A522V was expressed with a polyhistidine tag at the C terminus
    Catalog Number:
    40592-v08h16
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus spike Protein 2019-nCoV, cov spike Protein 2019-nCoV, ncov RBD Protein 2019-nCoV, ncov s1 Protein 2019-nCoV, ncov s2 Protein 2019-nCoV, ncov spike Protein 2019-nCoV, NCP-CoV RBD Protein 2019-nCoV, NCP-CoV s1 Protein 2019-nCoV, NCP-CoV s2 Protein 2019-nCoV, NCP-CoV Spike Protein 2019-nCoV, novel coronavirus RBD Protein 2019-nCoV, novel coronavirus s1 Protein 2019-nCoV, novel coronavirus s2 Protein 2019-nCoV, novel coronavirus spike Protein 2019-nCoV, RBD Protein 2019-nCoV, S1 Protein 2019-nCoV, S2 Protein 2019-nCoV, Spike RBD Protein 2019-nCoV
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological sars cov 2 rbd protein
    Neutralization activity of sera from mice immunized with RBD monomer or RBD-conjugated nanoparticles. (A) <t>SARS-CoV-2</t> pseudovirus neutralization assay showing the NT 90 . (B) SARS-CoV-2 live virus neutralization assay showing the FRNT 90 . The statistical significance of the difference of neutralizing titers of mice immunized with immunogen combined with either AddaVax or SAS as the adjuvant was calculated using the unpaired two-tailed nonparametric Mann–Whitney U test. * p
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike RBD A522V His Recombinant Protein YP 009724390 1 Arg319 Phe541 A522V was expressed with a polyhistidine tag at the C terminus
    https://www.bioz.com/result/sars cov 2 rbd protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 rbd protein - by Bioz Stars, 2021-03
    94/100 stars

    Images

    1) Product Images from "Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates"

    Article Title: Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates

    Journal: ACS Nano

    doi: 10.1021/acsnano.0c08379

    Neutralization activity of sera from mice immunized with RBD monomer or RBD-conjugated nanoparticles. (A) SARS-CoV-2 pseudovirus neutralization assay showing the NT 90 . (B) SARS-CoV-2 live virus neutralization assay showing the FRNT 90 . The statistical significance of the difference of neutralizing titers of mice immunized with immunogen combined with either AddaVax or SAS as the adjuvant was calculated using the unpaired two-tailed nonparametric Mann–Whitney U test. * p
    Figure Legend Snippet: Neutralization activity of sera from mice immunized with RBD monomer or RBD-conjugated nanoparticles. (A) SARS-CoV-2 pseudovirus neutralization assay showing the NT 90 . (B) SARS-CoV-2 live virus neutralization assay showing the FRNT 90 . The statistical significance of the difference of neutralizing titers of mice immunized with immunogen combined with either AddaVax or SAS as the adjuvant was calculated using the unpaired two-tailed nonparametric Mann–Whitney U test. * p

    Techniques Used: Neutralization, Activity Assay, Mouse Assay, Two Tailed Test, MANN-WHITNEY

    Related Articles

    Recombinant:

    Article Title: SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2
    Article Snippet: A highly purified commercial preparation of RBD protein was used in some studies (SINO Biologics, Suppl. .. Fig. S2A) as well as recombinant RBD protein expressed in ExpiHEK cells (Suppl. .. Fig. S2D), both of which were judged > 98% pure by SDS-PAGE and SEC (Suppl.

    Article Title: Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response
    Article Snippet: 3.2 S1-4 protein possessed antigenicity in vitro The antigenicity of S1-4 protein was demonstrated by WB analysis with a commercial recombinant RBD protein (Val16 - Arg685 of spike protein; 76.45 kDa) expressed in baculovirus-insect cells by Sino Biological (Beijing, China) as positive control ( ). .. Ten micrograms of S1-4 protein and commercial recombinant RBD protein were loaded onto the first three gels ( A – 4C), with the exception of 30 µg of the two proteins in the fourth gel ( D). .. Whether it was Rabbit monoclonal antibody against RBD ( B), Rat polyclonal antibody against inactivated SARS-CoV-2 ( C), or convalescent serum of COVID-19 patients ( D) as the primary antibody, there was an obvious band at about 30 kDa, suggesting the high antigenicity of S1-4 protein.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates
    Article Snippet: The thermal transition midpoint (T m ) and aggregation results from start to finish (T agg) were reported and analyzed in PR.ThermControl software (NanoTemper Technologies). .. Enzyme-Linked Immunosorbent Assay (ELISA) ELISA was performed to examine the binding ability of the purified SARS-CoV-2 RBD protein to the receptor ACE2 and the RBD-specific CB6 antibody. .. Purified RBD monomer and RBD-conjugated NPs were diluted to a concentration of 1 μg/mL to coat 96-well microplates (Corning) (100 μL/well) in triplicate overnight at 4 °C.

    Binding Assay:

    Article Title: Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates
    Article Snippet: The thermal transition midpoint (T m ) and aggregation results from start to finish (T agg) were reported and analyzed in PR.ThermControl software (NanoTemper Technologies). .. Enzyme-Linked Immunosorbent Assay (ELISA) ELISA was performed to examine the binding ability of the purified SARS-CoV-2 RBD protein to the receptor ACE2 and the RBD-specific CB6 antibody. .. Purified RBD monomer and RBD-conjugated NPs were diluted to a concentration of 1 μg/mL to coat 96-well microplates (Corning) (100 μL/well) in triplicate overnight at 4 °C.

    Purification:

    Article Title: Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates
    Article Snippet: The thermal transition midpoint (T m ) and aggregation results from start to finish (T agg) were reported and analyzed in PR.ThermControl software (NanoTemper Technologies). .. Enzyme-Linked Immunosorbent Assay (ELISA) ELISA was performed to examine the binding ability of the purified SARS-CoV-2 RBD protein to the receptor ACE2 and the RBD-specific CB6 antibody. .. Purified RBD monomer and RBD-conjugated NPs were diluted to a concentration of 1 μg/mL to coat 96-well microplates (Corning) (100 μL/well) in triplicate overnight at 4 °C.

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  • 94
    Sino Biological sars cov 2 rbd protein
    Neutralization activity of sera from mice immunized with RBD monomer or RBD-conjugated nanoparticles. (A) <t>SARS-CoV-2</t> pseudovirus neutralization assay showing the NT 90 . (B) SARS-CoV-2 live virus neutralization assay showing the FRNT 90 . The statistical significance of the difference of neutralizing titers of mice immunized with immunogen combined with either AddaVax or SAS as the adjuvant was calculated using the unpaired two-tailed nonparametric Mann–Whitney U test. * p
    Sars Cov 2 Rbd Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 rbd protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 rbd protein - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

    94
    Sino Biological sars cov 2 2019 ncov spike rbd a522v his recombinant protein
    Neutralization activity of sera from mice immunized with RBD monomer or RBD-conjugated nanoparticles. (A) <t>SARS-CoV-2</t> pseudovirus neutralization assay showing the NT 90 . (B) SARS-CoV-2 live virus neutralization assay showing the FRNT 90 . The statistical significance of the difference of neutralizing titers of mice immunized with immunogen combined with either AddaVax or SAS as the adjuvant was calculated using the unpaired two-tailed nonparametric Mann–Whitney U test. * p
    Sars Cov 2 2019 Ncov Spike Rbd A522v His Recombinant Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 2019 ncov spike rbd a522v his recombinant protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 2019 ncov spike rbd a522v his recombinant protein - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

    Image Search Results


    Neutralization activity of sera from mice immunized with RBD monomer or RBD-conjugated nanoparticles. (A) SARS-CoV-2 pseudovirus neutralization assay showing the NT 90 . (B) SARS-CoV-2 live virus neutralization assay showing the FRNT 90 . The statistical significance of the difference of neutralizing titers of mice immunized with immunogen combined with either AddaVax or SAS as the adjuvant was calculated using the unpaired two-tailed nonparametric Mann–Whitney U test. * p

    Journal: ACS Nano

    Article Title: Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates

    doi: 10.1021/acsnano.0c08379

    Figure Lengend Snippet: Neutralization activity of sera from mice immunized with RBD monomer or RBD-conjugated nanoparticles. (A) SARS-CoV-2 pseudovirus neutralization assay showing the NT 90 . (B) SARS-CoV-2 live virus neutralization assay showing the FRNT 90 . The statistical significance of the difference of neutralizing titers of mice immunized with immunogen combined with either AddaVax or SAS as the adjuvant was calculated using the unpaired two-tailed nonparametric Mann–Whitney U test. * p

    Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) ELISA was performed to examine the binding ability of the purified SARS-CoV-2 RBD protein to the receptor ACE2 and the RBD-specific CB6 antibody.

    Techniques: Neutralization, Activity Assay, Mouse Assay, Two Tailed Test, MANN-WHITNEY