sars cov 2 spike receptor binding domain rbd fc recombinant proteins  (Sino Biological)


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    SARS CoV 2 2019 nCoV Spike RBD Fc Recombinant Protein HPLC verified COVID 19 Spike RBD Research
    Description:
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 Arg319 Phe541 was expressed with the Fc region of human IgG1 at the C terminus
    Catalog Number:
    40592-V02H
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus spike Protein 2019-nCoV, cov spike Protein 2019-nCoV, ncov RBD Protein 2019-nCoV, ncov s1 Protein 2019-nCoV, ncov s2 Protein 2019-nCoV, ncov spike Protein 2019-nCoV, NCP-CoV RBD Protein 2019-nCoV, NCP-CoV s1 Protein 2019-nCoV, NCP-CoV s2 Protein 2019-nCoV, NCP-CoV Spike Protein 2019-nCoV, novel coronavirus RBD Protein 2019-nCoV, novel coronavirus s1 Protein 2019-nCoV, novel coronavirus s2 Protein 2019-nCoV, novel coronavirus spike Protein 2019-nCoV, RBD Protein 2019-nCoV, S1 Protein 2019-nCoV, S2 Protein 2019-nCoV, Spike RBD Protein 2019-nCoV
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological sars cov 2 spike receptor binding domain rbd fc recombinant proteins
    Nanodecoys inhibit pseudovirus and authentic <t>SARS-CoV-2</t> infection. Inhibitory activity of nanodecoys against PsV ( A ) SARS-CoV-2, ( B ) SARS-CoV, ( C ) WIV1, and ( D ) Rs3367 infection. ( E ) Immunofluorescence images of SARS-CoV-2−infected Vero-E6 cells after treatment with nanodecoys. (Scale bars, 100 µm.) Cell nuclei and N protein of SARS-CoV-2 were labeled with DAPI (blue) and Alexa 488 (green), respectively. ( F ) Inhibitory activity of nanodecoys against SARS-CoV-2 infection on Vero-E6 cells. The nanodecoys used in this experiment contained an equal amount of ACE2 as compared with ACE2-Ves. Data points represent mean ± SD ( n = 3). As compared with the 293T-Ves group, ns and *** indicate no statistical difference and P
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 Arg319 Phe541 was expressed with the Fc region of human IgG1 at the C terminus
    https://www.bioz.com/result/sars cov 2 spike receptor binding domain rbd fc recombinant proteins/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 spike receptor binding domain rbd fc recombinant proteins - by Bioz Stars, 2021-07
    94/100 stars

    Images

    1) Product Images from "Decoy nanoparticles protect against COVID-19 by concurrently adsorbing viruses and inflammatory cytokines"

    Article Title: Decoy nanoparticles protect against COVID-19 by concurrently adsorbing viruses and inflammatory cytokines

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2014352117

    Nanodecoys inhibit pseudovirus and authentic SARS-CoV-2 infection. Inhibitory activity of nanodecoys against PsV ( A ) SARS-CoV-2, ( B ) SARS-CoV, ( C ) WIV1, and ( D ) Rs3367 infection. ( E ) Immunofluorescence images of SARS-CoV-2−infected Vero-E6 cells after treatment with nanodecoys. (Scale bars, 100 µm.) Cell nuclei and N protein of SARS-CoV-2 were labeled with DAPI (blue) and Alexa 488 (green), respectively. ( F ) Inhibitory activity of nanodecoys against SARS-CoV-2 infection on Vero-E6 cells. The nanodecoys used in this experiment contained an equal amount of ACE2 as compared with ACE2-Ves. Data points represent mean ± SD ( n = 3). As compared with the 293T-Ves group, ns and *** indicate no statistical difference and P
    Figure Legend Snippet: Nanodecoys inhibit pseudovirus and authentic SARS-CoV-2 infection. Inhibitory activity of nanodecoys against PsV ( A ) SARS-CoV-2, ( B ) SARS-CoV, ( C ) WIV1, and ( D ) Rs3367 infection. ( E ) Immunofluorescence images of SARS-CoV-2−infected Vero-E6 cells after treatment with nanodecoys. (Scale bars, 100 µm.) Cell nuclei and N protein of SARS-CoV-2 were labeled with DAPI (blue) and Alexa 488 (green), respectively. ( F ) Inhibitory activity of nanodecoys against SARS-CoV-2 infection on Vero-E6 cells. The nanodecoys used in this experiment contained an equal amount of ACE2 as compared with ACE2-Ves. Data points represent mean ± SD ( n = 3). As compared with the 293T-Ves group, ns and *** indicate no statistical difference and P

    Techniques Used: Infection, Activity Assay, Immunofluorescence, Labeling

    2) Product Images from "Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19"

    Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19

    Journal: Clinical Science (London, England : 1979)

    doi: 10.1042/CS20201511

    Effects of candesartan and captopril on spike protein internalization in cultures of human alveolar type-II A549 cells Effects of candesartan and captopril on SARS-CoV-2 Spike RBD-Fc internalization rate by measuring cytoplasmic fluorescence intensities of spike protein using confocal laser microscopy ( A ). ACE2-GFP (green), spike protein (red) and merge (yellow) fluorescence in untreated controls ( B–D ), and cultures treated with spike protein alone ( E–G ) or spike protein and candesartan ( H–J ) or spike protein and captopril ( K–M ). * P
    Figure Legend Snippet: Effects of candesartan and captopril on spike protein internalization in cultures of human alveolar type-II A549 cells Effects of candesartan and captopril on SARS-CoV-2 Spike RBD-Fc internalization rate by measuring cytoplasmic fluorescence intensities of spike protein using confocal laser microscopy ( A ). ACE2-GFP (green), spike protein (red) and merge (yellow) fluorescence in untreated controls ( B–D ), and cultures treated with spike protein alone ( E–G ) or spike protein and candesartan ( H–J ) or spike protein and captopril ( K–M ). * P

    Techniques Used: Fluorescence, Microscopy

    Diagram summarizing major results and conclusions Candesartan and captopril increase ACE2 levels and anti-inflammatory RAS arm activity, and inhibit the proinflammatory RAS axis. This leads to anti-inflammatory, anti-fibrotic and anti-thrombotic effects in the lung, and counteracts the opposite effects of aging and metabolic syndrome (obesity, hyperglycemia and hypertension) and SARS-CoV-2/spike protein on the same RAS components. The candesartan/captopril-induced increase in ACE2 levels leads to an increase in levels of viral receptors. However, a simultaneous candesartan/captopril-induced reduction in ADAM17 activity reduces viral entry and cell infection.
    Figure Legend Snippet: Diagram summarizing major results and conclusions Candesartan and captopril increase ACE2 levels and anti-inflammatory RAS arm activity, and inhibit the proinflammatory RAS axis. This leads to anti-inflammatory, anti-fibrotic and anti-thrombotic effects in the lung, and counteracts the opposite effects of aging and metabolic syndrome (obesity, hyperglycemia and hypertension) and SARS-CoV-2/spike protein on the same RAS components. The candesartan/captopril-induced increase in ACE2 levels leads to an increase in levels of viral receptors. However, a simultaneous candesartan/captopril-induced reduction in ADAM17 activity reduces viral entry and cell infection.

    Techniques Used: Activity Assay, Infection

    3) Product Images from "Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19"

    Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19

    Journal: Clinical Science (London, England : 1979)

    doi: 10.1042/CS20201511

    Effects of candesartan and captopril on spike protein internalization in cultures of human alveolar type-II A549 cells Effects of candesartan and captopril on SARS-CoV-2 Spike RBD-Fc internalization rate by measuring cytoplasmic fluorescence intensities of spike protein using confocal laser microscopy ( A ). ACE2-GFP (green), spike protein (red) and merge (yellow) fluorescence in untreated controls ( B–D ), and cultures treated with spike protein alone ( E–G ) or spike protein and candesartan ( H–J ) or spike protein and captopril ( K–M ). * P
    Figure Legend Snippet: Effects of candesartan and captopril on spike protein internalization in cultures of human alveolar type-II A549 cells Effects of candesartan and captopril on SARS-CoV-2 Spike RBD-Fc internalization rate by measuring cytoplasmic fluorescence intensities of spike protein using confocal laser microscopy ( A ). ACE2-GFP (green), spike protein (red) and merge (yellow) fluorescence in untreated controls ( B–D ), and cultures treated with spike protein alone ( E–G ) or spike protein and candesartan ( H–J ) or spike protein and captopril ( K–M ). * P

    Techniques Used: Fluorescence, Microscopy

    Diagram summarizing major results and conclusions Candesartan and captopril increase ACE2 levels and anti-inflammatory RAS arm activity, and inhibit the proinflammatory RAS axis. This leads to anti-inflammatory, anti-fibrotic and anti-thrombotic effects in the lung, and counteracts the opposite effects of aging and metabolic syndrome (obesity, hyperglycemia and hypertension) and SARS-CoV-2/spike protein on the same RAS components. The candesartan/captopril-induced increase in ACE2 levels leads to an increase in levels of viral receptors. However, a simultaneous candesartan/captopril-induced reduction in ADAM17 activity reduces viral entry and cell infection.
    Figure Legend Snippet: Diagram summarizing major results and conclusions Candesartan and captopril increase ACE2 levels and anti-inflammatory RAS arm activity, and inhibit the proinflammatory RAS axis. This leads to anti-inflammatory, anti-fibrotic and anti-thrombotic effects in the lung, and counteracts the opposite effects of aging and metabolic syndrome (obesity, hyperglycemia and hypertension) and SARS-CoV-2/spike protein on the same RAS components. The candesartan/captopril-induced increase in ACE2 levels leads to an increase in levels of viral receptors. However, a simultaneous candesartan/captopril-induced reduction in ADAM17 activity reduces viral entry and cell infection.

    Techniques Used: Activity Assay, Infection

    4) Product Images from "Variable Induction of Pro-inflammatory Cytokines by Commercial SARS CoV-2 Spike Protein Reagents: Potential Impacts of LPS on In Vitro Modeling and Pathogenic Mechanisms In Vivo"

    Article Title: Variable Induction of Pro-inflammatory Cytokines by Commercial SARS CoV-2 Spike Protein Reagents: Potential Impacts of LPS on In Vitro Modeling and Pathogenic Mechanisms In Vivo

    Journal: bioRxiv

    doi: 10.1101/2021.05.26.445843

    SARS CoV-2 spike protein-induced cytokine production is independent of its binding to ACE2. ( A ) The binding activities of coronaviral spike Fc fusion proteins to ACE2 were measured using an ELISA. The binding activities of 16, 80, 400 and 2000 ng/mL of the indicated proteins were presented as absorbance at 450 nm (O.D. 450). ( B ) Inhibition of S1-Fc binding to ACE2 by soluble ACE2 or a neutralizing anti-S1 antibody. 400 ng/mL of S1-Fc was preincubated with or without 2 μg/mL of soluble ACE2, 1 μg/mL of neutralizing (nAb) or non-neutralizing (non-nAb) anti-S1 antibody before incubation with plate-bound ACE2. Statistical analyses were performed using a two-tailed, Student’s T-test. *** depicts p
    Figure Legend Snippet: SARS CoV-2 spike protein-induced cytokine production is independent of its binding to ACE2. ( A ) The binding activities of coronaviral spike Fc fusion proteins to ACE2 were measured using an ELISA. The binding activities of 16, 80, 400 and 2000 ng/mL of the indicated proteins were presented as absorbance at 450 nm (O.D. 450). ( B ) Inhibition of S1-Fc binding to ACE2 by soluble ACE2 or a neutralizing anti-S1 antibody. 400 ng/mL of S1-Fc was preincubated with or without 2 μg/mL of soluble ACE2, 1 μg/mL of neutralizing (nAb) or non-neutralizing (non-nAb) anti-S1 antibody before incubation with plate-bound ACE2. Statistical analyses were performed using a two-tailed, Student’s T-test. *** depicts p

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Inhibition, Incubation, Two Tailed Test

    Cytokine responses in human PBMC induced by various commercial coronavirus spike proteins. ( A ) Rested PBMC were cultured with or without 2.0 μg/mL of raxibacumab (human anti-anthrax PA IgG used as a negative control), MERS S1-Fc, SARS CoV-1 S1-Fc, SARS CoV-2 S1-Fc from Vendor #2 (V#2 S1-Fc) and Vendor #1 (lot 24529-2003, V#1 S1-Fc 2003), and RBD-Fc from Vendor #2 (V#2 RBD-Fc) and Vendor #1 (lot 24530-2003, V#1 RBD-Fc 2003) for 24 hours. ( B ) Rested PBMC were cultured with or without plate-bound streptavidin (STAV) together with or without S1-biotin and RBD-biotin purchased from Vendor #2. The levels of IL-6 and TNFα were measured using the CBA human inflammatory cytokine kit and flow cytometric analysis. IL-8 levels were measured using an ELISA kit. The concentrations of the cytokines were calculated based on the standard curves, and the induction of cytokines were presented as stimulation indices. Data shown are statistical results (mean ± SE) generated from 8 ( A ) or 3 ( B ) healthy donors. Statistical analyses were performed by Excel using a two-tailed, Student’s T-test. *, **, *** and **** depict p
    Figure Legend Snippet: Cytokine responses in human PBMC induced by various commercial coronavirus spike proteins. ( A ) Rested PBMC were cultured with or without 2.0 μg/mL of raxibacumab (human anti-anthrax PA IgG used as a negative control), MERS S1-Fc, SARS CoV-1 S1-Fc, SARS CoV-2 S1-Fc from Vendor #2 (V#2 S1-Fc) and Vendor #1 (lot 24529-2003, V#1 S1-Fc 2003), and RBD-Fc from Vendor #2 (V#2 RBD-Fc) and Vendor #1 (lot 24530-2003, V#1 RBD-Fc 2003) for 24 hours. ( B ) Rested PBMC were cultured with or without plate-bound streptavidin (STAV) together with or without S1-biotin and RBD-biotin purchased from Vendor #2. The levels of IL-6 and TNFα were measured using the CBA human inflammatory cytokine kit and flow cytometric analysis. IL-8 levels were measured using an ELISA kit. The concentrations of the cytokines were calculated based on the standard curves, and the induction of cytokines were presented as stimulation indices. Data shown are statistical results (mean ± SE) generated from 8 ( A ) or 3 ( B ) healthy donors. Statistical analyses were performed by Excel using a two-tailed, Student’s T-test. *, **, *** and **** depict p

    Techniques Used: Cell Culture, Negative Control, Crocin Bleaching Assay, Enzyme-linked Immunosorbent Assay, Generated, Two Tailed Test

    Dose-dependent cytokine responses of human PBMC induced by SARS CoV-2 S1-Fc fusion protein. Rested PBMC from two healthy donors (HD1 and HD2) were cultured for 48 hours with 0, 0.4, 1.0 and 2.5 μg/mL of S1-Fc purchased from Vendor #1 (lot 24056-2002-2). The levels of IL-1β, IL-6, IL-8, IL-10, IL-12 and TNFα in the supernatants of cultured PBMC were assessed using the CBA human inflammatory cytokine kit and flow cytometric analysis. Data shown are representative of the flow cytometric results from two independent experiments with total three healthy donors.
    Figure Legend Snippet: Dose-dependent cytokine responses of human PBMC induced by SARS CoV-2 S1-Fc fusion protein. Rested PBMC from two healthy donors (HD1 and HD2) were cultured for 48 hours with 0, 0.4, 1.0 and 2.5 μg/mL of S1-Fc purchased from Vendor #1 (lot 24056-2002-2). The levels of IL-1β, IL-6, IL-8, IL-10, IL-12 and TNFα in the supernatants of cultured PBMC were assessed using the CBA human inflammatory cytokine kit and flow cytometric analysis. Data shown are representative of the flow cytometric results from two independent experiments with total three healthy donors.

    Techniques Used: Cell Culture, Crocin Bleaching Assay

    Related Articles

    Transfection:

    Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19
    Article Snippet: Cells were transiently transfected with 2 μg of ACE2 cDNA (ACE2 tGFP-tagged, RG208442, Origene) using a commercial transfection reagent, Turbofect (R0533, Thermo Scientific). .. SARS-CoV-2 Spike RBD-Fc protein internalization assayHuman alveolar type-II pneumocyte cell lines, A549, transiently transfected with tGFP ACE2 were treated or not treated with Candesartan or Captopril for 24 h. Then, 1 µg/ml of SARS-CoV-2 Spike RBD-Fc protein (40592-V02H, Sino Biological) was added to the cells for 3 h at 37°C. .. Cells were fixed and incubated overnight at 4°C with a mouse monoclonal antibody against human IgG-Fc (ab99757, abcam, 1:500) diluted in DPBS containing 1% BSA, 2% normal goat serum and 0.05% Triton X-100.

    Recombinant:

    Article Title: Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men
    Article Snippet: .. After 24 hours, 0.5 μM/mL human Fc-tagged recombinant spike-RBD protein with (Sino Biological Inc. 40592-V02H) was added to the medium and incubated for 30 minutes. .. The cells were subsequently fixed, permeabilized and stained with antibodies against ACE2 and human Fc receptor.

    Article Title: Decoy nanoparticles protect against COVID-19 by concurrently adsorbing viruses and inflammatory cytokines
    Article Snippet: Immunofluorescence.To confirm the ACE2 on engineered 293T cells, parental and engineered 293T cells were plated into glass-bottomed dishes. .. After overnight culture, the cells were incubated with 1 μg/mL SARS-CoV-2 spike receptor-binding domain (RBD)-Fc recombinant proteins (Sino Biological) at 25 °C for 30 min. .. The cells were then stained with phycoerythrin (PE)-conjugated donkey anti-human IgG (Jackson ImmunoResearch) at 4 °C for 20 min. After being stained with DAPI, the cells were finally observed under confocal laser scanning microscopy (CLSM; ZEISS LSM700).

    Article Title: Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men
    Article Snippet: .. After 24 h, 0.5 μM/mL human Fc-tagged recombinant spike-RBD protein (Sino Biological Inc. 40592-V02H) was added to the medium and incubated for 30 min. .. The cells were subsequently fixed, permeabilized, and stained with antibodies against ACE2 and human Fc receptor, as described previously, followed by high-throughput imaging and quantification using the In Cell Analyzer 2000 (GE Healthcare, USA).

    Incubation:

    Article Title: Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men
    Article Snippet: .. After 24 hours, 0.5 μM/mL human Fc-tagged recombinant spike-RBD protein with (Sino Biological Inc. 40592-V02H) was added to the medium and incubated for 30 minutes. .. The cells were subsequently fixed, permeabilized and stained with antibodies against ACE2 and human Fc receptor.

    Article Title: Decoy nanoparticles protect against COVID-19 by concurrently adsorbing viruses and inflammatory cytokines
    Article Snippet: Immunofluorescence.To confirm the ACE2 on engineered 293T cells, parental and engineered 293T cells were plated into glass-bottomed dishes. .. After overnight culture, the cells were incubated with 1 μg/mL SARS-CoV-2 spike receptor-binding domain (RBD)-Fc recombinant proteins (Sino Biological) at 25 °C for 30 min. .. The cells were then stained with phycoerythrin (PE)-conjugated donkey anti-human IgG (Jackson ImmunoResearch) at 4 °C for 20 min. After being stained with DAPI, the cells were finally observed under confocal laser scanning microscopy (CLSM; ZEISS LSM700).

    Article Title: Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men
    Article Snippet: .. After 24 h, 0.5 μM/mL human Fc-tagged recombinant spike-RBD protein (Sino Biological Inc. 40592-V02H) was added to the medium and incubated for 30 min. .. The cells were subsequently fixed, permeabilized, and stained with antibodies against ACE2 and human Fc receptor, as described previously, followed by high-throughput imaging and quantification using the In Cell Analyzer 2000 (GE Healthcare, USA).

    other:

    Article Title: Variable Induction of Pro-inflammatory Cytokines by Commercial SARS CoV-2 Spike Protein Reagents: Potential Impacts of LPS on In Vitro Modeling and Pathogenic Mechanisms In Vivo
    Article Snippet: S1-Fc (Catalog# 40591-V02H, lot LC14AP1605), RBD-Fc (Catalog# 40592-V02H, lot LC14MC2602), S1-biotin (Catalog# 40591-V27HB, lot LC14AU101), RBD-biotin (Catalog# 40592-V27B-B, lot MF14AP2302), ACE2 (Catalog# 10108-H08H, lot MB14JN0906), anti-S1 neutralizing antibody (Catalog# 40591-MM43, lot HB14AP2001) and anti-S1 non-neutralizing antibody (Catalog # 40591-MM42, lot HB14AP0701) were also purchased from Sino Biological (Vendor #2).

    Clone Assay:

    Article Title: Antibodies that potently inhibit or enhance SARS-CoV-2 spike protein-ACE2 interaction isolated from synthetic single-chain antibody libraries
    Article Snippet: The library was panned using either SARS-CoV-2 RBD (Sino Biological, Cat: 40592-V08H; screens RU167 and RU169) or soluble S1 ectodomain trimer (Sino Biological, Cat: 40591-V08H; RU171) that was biotinylated using ChromaLINK Biotin (Vector Laboratories, Cat: B-1001) and attached to MyOne Streptavidin C1 Dynabeads (ThermoFisher, Cat: 65002). .. RBD or S1-binding clones were moved into the ReD cell-display platform (Beasley et al. 2015) and cells were membrane permeabilized using 0.5% n-octyl b-d-thioglucopyranoside (Anatrace, Cat: 0314) and labeled for FACS using either SARS-CoV-2 RBD-Fc (Sino Biological, Cat: 40592-V02H; screens RU167 and RU169) or soluble S1 ectodomain trimer (Sino Biological, Cat: 40591-V08H; RU171) that had been fluorophore labeled with either ATTO 488 NHS ester (Merck, Cat: 41698) or Dy-549P1 (Dyomics, Cat: 549P1-01). ..

    Labeling:

    Article Title: Antibodies that potently inhibit or enhance SARS-CoV-2 spike protein-ACE2 interaction isolated from synthetic single-chain antibody libraries
    Article Snippet: The library was panned using either SARS-CoV-2 RBD (Sino Biological, Cat: 40592-V08H; screens RU167 and RU169) or soluble S1 ectodomain trimer (Sino Biological, Cat: 40591-V08H; RU171) that was biotinylated using ChromaLINK Biotin (Vector Laboratories, Cat: B-1001) and attached to MyOne Streptavidin C1 Dynabeads (ThermoFisher, Cat: 65002). .. RBD or S1-binding clones were moved into the ReD cell-display platform (Beasley et al. 2015) and cells were membrane permeabilized using 0.5% n-octyl b-d-thioglucopyranoside (Anatrace, Cat: 0314) and labeled for FACS using either SARS-CoV-2 RBD-Fc (Sino Biological, Cat: 40592-V02H; screens RU167 and RU169) or soluble S1 ectodomain trimer (Sino Biological, Cat: 40591-V08H; RU171) that had been fluorophore labeled with either ATTO 488 NHS ester (Merck, Cat: 41698) or Dy-549P1 (Dyomics, Cat: 549P1-01). ..

    FACS:

    Article Title: Antibodies that potently inhibit or enhance SARS-CoV-2 spike protein-ACE2 interaction isolated from synthetic single-chain antibody libraries
    Article Snippet: The library was panned using either SARS-CoV-2 RBD (Sino Biological, Cat: 40592-V08H; screens RU167 and RU169) or soluble S1 ectodomain trimer (Sino Biological, Cat: 40591-V08H; RU171) that was biotinylated using ChromaLINK Biotin (Vector Laboratories, Cat: B-1001) and attached to MyOne Streptavidin C1 Dynabeads (ThermoFisher, Cat: 65002). .. RBD or S1-binding clones were moved into the ReD cell-display platform (Beasley et al. 2015) and cells were membrane permeabilized using 0.5% n-octyl b-d-thioglucopyranoside (Anatrace, Cat: 0314) and labeled for FACS using either SARS-CoV-2 RBD-Fc (Sino Biological, Cat: 40592-V02H; screens RU167 and RU169) or soluble S1 ectodomain trimer (Sino Biological, Cat: 40591-V08H; RU171) that had been fluorophore labeled with either ATTO 488 NHS ester (Merck, Cat: 41698) or Dy-549P1 (Dyomics, Cat: 549P1-01). ..

    Binding Assay:

    Article Title: Detection of SARS-CoV-2 neutralizing antibodies with a cell-free PCR assay
    Article Snippet: The SARS-CoV-2 spike protein (S1) containing amino acids 1-674 with an Fc-tag at the C-terminus (#31806) expressed in HEK293 cells was purchased from the Native Antigen Company (Oxford, United Kingdom). .. The SARS-CoV-2 spike protein receptor binding domain (RBD) containing amino acids 319-541 with an Fc-tag at the Cterminus (#40592-V02H) and the human receptor angiotensin-converting enzyme 2 (ACE2) protein containing amino acids 1-740 with an Fc-tag at the C-terminus (10108- H05H) expressed in HEK293 cells were obtained from Sino Biologicals (Beijing, China). ..

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    Sino Biological sars cov 2 spike receptor binding domain rbd fc recombinant proteins
    Nanodecoys inhibit pseudovirus and authentic <t>SARS-CoV-2</t> infection. Inhibitory activity of nanodecoys against PsV ( A ) SARS-CoV-2, ( B ) SARS-CoV, ( C ) WIV1, and ( D ) Rs3367 infection. ( E ) Immunofluorescence images of SARS-CoV-2−infected Vero-E6 cells after treatment with nanodecoys. (Scale bars, 100 µm.) Cell nuclei and N protein of SARS-CoV-2 were labeled with DAPI (blue) and Alexa 488 (green), respectively. ( F ) Inhibitory activity of nanodecoys against SARS-CoV-2 infection on Vero-E6 cells. The nanodecoys used in this experiment contained an equal amount of ACE2 as compared with ACE2-Ves. Data points represent mean ± SD ( n = 3). As compared with the 293T-Ves group, ns and *** indicate no statistical difference and P
    Sars Cov 2 Spike Receptor Binding Domain Rbd Fc Recombinant Proteins, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 spike receptor binding domain rbd fc recombinant proteins/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Sino Biological rbd fc
    Scheme describing the assays employed in this paper. (A) The recombinant protein constructs ACE2-His-Avi (ACE2-Avi) (Acro Biosystems) and <t>SARS-CoV-2</t> spike protein receptor binding domain-Fc <t>(RBD-Fc)</t> (Sino Biological) were used to model ACE2–RBD binding. (B) AlphaLISA assay system used to monitor ACE2–RBD interacts. Streptavidin donor beads recognize the Avi tag on ACE2. The protein A acceptor beads recognize the Fc tag on RBD. When in proximity the donor beads can be excited with light at 680 nm. This generates singlet oxygen which diffuses to the acceptor beads, causing the acceptor beads to luminesce at 615 nm. (C) The TruHits counterscreen uses streptavidindonor beads which directly interact with biotin acceptor beads. Because no intermediary molecule is needed to bring the donor and acceptor beads in proximity, the TruHits assay can identify compounds which directly interfere with the AlphaLISA readout.
    Rbd Fc, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rbd fc/product/Sino Biological
    Average 86 stars, based on 1 article reviews
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    Sino Biological sars cov 2 2019 ncov spike rbd fc recombinant protein hplc verified covid 19 spike rbd research
    Combined prognostic biomarker and serology detection. ( A ) Dose-response curve for recombinant IP-10 spiked into FBS. Each data point represents the average ( n = 3), and error bars represent the SEM. The limit of detection (LOD) for IP-10 is 0.12 ng/ml. ( B ) Dose-response curve for <t>anti–SARS-CoV-2</t> antibodies spiked into FBS. The highest concentration is 10 μg/ml of anti-S1/RBD and 10 μg/ml of anti-N antibodies. Each data point represents the average ( n = 3) with SEM. ( C ) Correlation between DA-D4 readout for IP-10 with an ELISA performed separately. Samples with a letter designate samples from one individual at different time points, where b occurs later in disease than a. All samples were tested in duplicate on the DA-D4 (with SD shown) except 2b (due to insufficient volume). The solid line shows linear regression. ( D ) Antibody reactivity against S1, RBD, and N for sample tested in (C) (with SD shown). NC, negative control pooled healthy plasma.
    Sars Cov 2 2019 Ncov Spike Rbd Fc Recombinant Protein Hplc Verified Covid 19 Spike Rbd Research, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 2019 ncov spike rbd fc recombinant protein hplc verified covid 19 spike rbd research/product/Sino Biological
    Average 86 stars, based on 1 article reviews
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    Nanodecoys inhibit pseudovirus and authentic SARS-CoV-2 infection. Inhibitory activity of nanodecoys against PsV ( A ) SARS-CoV-2, ( B ) SARS-CoV, ( C ) WIV1, and ( D ) Rs3367 infection. ( E ) Immunofluorescence images of SARS-CoV-2−infected Vero-E6 cells after treatment with nanodecoys. (Scale bars, 100 µm.) Cell nuclei and N protein of SARS-CoV-2 were labeled with DAPI (blue) and Alexa 488 (green), respectively. ( F ) Inhibitory activity of nanodecoys against SARS-CoV-2 infection on Vero-E6 cells. The nanodecoys used in this experiment contained an equal amount of ACE2 as compared with ACE2-Ves. Data points represent mean ± SD ( n = 3). As compared with the 293T-Ves group, ns and *** indicate no statistical difference and P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Decoy nanoparticles protect against COVID-19 by concurrently adsorbing viruses and inflammatory cytokines

    doi: 10.1073/pnas.2014352117

    Figure Lengend Snippet: Nanodecoys inhibit pseudovirus and authentic SARS-CoV-2 infection. Inhibitory activity of nanodecoys against PsV ( A ) SARS-CoV-2, ( B ) SARS-CoV, ( C ) WIV1, and ( D ) Rs3367 infection. ( E ) Immunofluorescence images of SARS-CoV-2−infected Vero-E6 cells after treatment with nanodecoys. (Scale bars, 100 µm.) Cell nuclei and N protein of SARS-CoV-2 were labeled with DAPI (blue) and Alexa 488 (green), respectively. ( F ) Inhibitory activity of nanodecoys against SARS-CoV-2 infection on Vero-E6 cells. The nanodecoys used in this experiment contained an equal amount of ACE2 as compared with ACE2-Ves. Data points represent mean ± SD ( n = 3). As compared with the 293T-Ves group, ns and *** indicate no statistical difference and P

    Article Snippet: After overnight culture, the cells were incubated with 1 μg/mL SARS-CoV-2 spike receptor-binding domain (RBD)-Fc recombinant proteins (Sino Biological) at 25 °C for 30 min.

    Techniques: Infection, Activity Assay, Immunofluorescence, Labeling

    Effects of candesartan and captopril on spike protein internalization in cultures of human alveolar type-II A549 cells Effects of candesartan and captopril on SARS-CoV-2 Spike RBD-Fc internalization rate by measuring cytoplasmic fluorescence intensities of spike protein using confocal laser microscopy ( A ). ACE2-GFP (green), spike protein (red) and merge (yellow) fluorescence in untreated controls ( B–D ), and cultures treated with spike protein alone ( E–G ) or spike protein and candesartan ( H–J ) or spike protein and captopril ( K–M ). * P

    Journal: Clinical Science (London, England : 1979)

    Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19

    doi: 10.1042/CS20201511

    Figure Lengend Snippet: Effects of candesartan and captopril on spike protein internalization in cultures of human alveolar type-II A549 cells Effects of candesartan and captopril on SARS-CoV-2 Spike RBD-Fc internalization rate by measuring cytoplasmic fluorescence intensities of spike protein using confocal laser microscopy ( A ). ACE2-GFP (green), spike protein (red) and merge (yellow) fluorescence in untreated controls ( B–D ), and cultures treated with spike protein alone ( E–G ) or spike protein and candesartan ( H–J ) or spike protein and captopril ( K–M ). * P

    Article Snippet: SARS-CoV-2 Spike RBD-Fc protein internalization assayHuman alveolar type-II pneumocyte cell lines, A549, transiently transfected with tGFP ACE2 were treated or not treated with Candesartan or Captopril for 24 h. Then, 1 µg/ml of SARS-CoV-2 Spike RBD-Fc protein (40592-V02H, Sino Biological) was added to the cells for 3 h at 37°C.

    Techniques: Fluorescence, Microscopy

    Diagram summarizing major results and conclusions Candesartan and captopril increase ACE2 levels and anti-inflammatory RAS arm activity, and inhibit the proinflammatory RAS axis. This leads to anti-inflammatory, anti-fibrotic and anti-thrombotic effects in the lung, and counteracts the opposite effects of aging and metabolic syndrome (obesity, hyperglycemia and hypertension) and SARS-CoV-2/spike protein on the same RAS components. The candesartan/captopril-induced increase in ACE2 levels leads to an increase in levels of viral receptors. However, a simultaneous candesartan/captopril-induced reduction in ADAM17 activity reduces viral entry and cell infection.

    Journal: Clinical Science (London, England : 1979)

    Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19

    doi: 10.1042/CS20201511

    Figure Lengend Snippet: Diagram summarizing major results and conclusions Candesartan and captopril increase ACE2 levels and anti-inflammatory RAS arm activity, and inhibit the proinflammatory RAS axis. This leads to anti-inflammatory, anti-fibrotic and anti-thrombotic effects in the lung, and counteracts the opposite effects of aging and metabolic syndrome (obesity, hyperglycemia and hypertension) and SARS-CoV-2/spike protein on the same RAS components. The candesartan/captopril-induced increase in ACE2 levels leads to an increase in levels of viral receptors. However, a simultaneous candesartan/captopril-induced reduction in ADAM17 activity reduces viral entry and cell infection.

    Article Snippet: SARS-CoV-2 Spike RBD-Fc protein internalization assayHuman alveolar type-II pneumocyte cell lines, A549, transiently transfected with tGFP ACE2 were treated or not treated with Candesartan or Captopril for 24 h. Then, 1 µg/ml of SARS-CoV-2 Spike RBD-Fc protein (40592-V02H, Sino Biological) was added to the cells for 3 h at 37°C.

    Techniques: Activity Assay, Infection

    Scheme describing the assays employed in this paper. (A) The recombinant protein constructs ACE2-His-Avi (ACE2-Avi) (Acro Biosystems) and SARS-CoV-2 spike protein receptor binding domain-Fc (RBD-Fc) (Sino Biological) were used to model ACE2–RBD binding. (B) AlphaLISA assay system used to monitor ACE2–RBD interacts. Streptavidin donor beads recognize the Avi tag on ACE2. The protein A acceptor beads recognize the Fc tag on RBD. When in proximity the donor beads can be excited with light at 680 nm. This generates singlet oxygen which diffuses to the acceptor beads, causing the acceptor beads to luminesce at 615 nm. (C) The TruHits counterscreen uses streptavidindonor beads which directly interact with biotin acceptor beads. Because no intermediary molecule is needed to bring the donor and acceptor beads in proximity, the TruHits assay can identify compounds which directly interfere with the AlphaLISA readout.

    Journal: ACS Pharmacology & Translational Science

    Article Title: Targeting ACE2–RBD Interaction as a Platform for COVID-19 Therapeutics: Development and Drug-Repurposing Screen of an AlphaLISA Proximity Assay

    doi: 10.1021/acsptsci.0c00161

    Figure Lengend Snippet: Scheme describing the assays employed in this paper. (A) The recombinant protein constructs ACE2-His-Avi (ACE2-Avi) (Acro Biosystems) and SARS-CoV-2 spike protein receptor binding domain-Fc (RBD-Fc) (Sino Biological) were used to model ACE2–RBD binding. (B) AlphaLISA assay system used to monitor ACE2–RBD interacts. Streptavidin donor beads recognize the Avi tag on ACE2. The protein A acceptor beads recognize the Fc tag on RBD. When in proximity the donor beads can be excited with light at 680 nm. This generates singlet oxygen which diffuses to the acceptor beads, causing the acceptor beads to luminesce at 615 nm. (C) The TruHits counterscreen uses streptavidindonor beads which directly interact with biotin acceptor beads. Because no intermediary molecule is needed to bring the donor and acceptor beads in proximity, the TruHits assay can identify compounds which directly interfere with the AlphaLISA readout.

    Article Snippet: RBD-Fc (SARS-CoV-2 spike protein residues 319–541, C-terminal Fc tag; catalog no.40592-V02H), ACE2-His (ACE2 residues 1–740, C-terminal His tag; catalog no. 10108-H08H), and S1-His (spike protein residues 1–667, C-terminal His tag; catalog no. 40150-V08B1) were acquired from Sino Biological (Wayne, PA).

    Techniques: Recombinant, Construct, Binding Assay

    Combined prognostic biomarker and serology detection. ( A ) Dose-response curve for recombinant IP-10 spiked into FBS. Each data point represents the average ( n = 3), and error bars represent the SEM. The limit of detection (LOD) for IP-10 is 0.12 ng/ml. ( B ) Dose-response curve for anti–SARS-CoV-2 antibodies spiked into FBS. The highest concentration is 10 μg/ml of anti-S1/RBD and 10 μg/ml of anti-N antibodies. Each data point represents the average ( n = 3) with SEM. ( C ) Correlation between DA-D4 readout for IP-10 with an ELISA performed separately. Samples with a letter designate samples from one individual at different time points, where b occurs later in disease than a. All samples were tested in duplicate on the DA-D4 (with SD shown) except 2b (due to insufficient volume). The solid line shows linear regression. ( D ) Antibody reactivity against S1, RBD, and N for sample tested in (C) (with SD shown). NC, negative control pooled healthy plasma.

    Journal: Science Advances

    Article Title: Multiplexed, quantitative serological profiling of COVID-19 from blood by a point-of-care test

    doi: 10.1126/sciadv.abg4901

    Figure Lengend Snippet: Combined prognostic biomarker and serology detection. ( A ) Dose-response curve for recombinant IP-10 spiked into FBS. Each data point represents the average ( n = 3), and error bars represent the SEM. The limit of detection (LOD) for IP-10 is 0.12 ng/ml. ( B ) Dose-response curve for anti–SARS-CoV-2 antibodies spiked into FBS. The highest concentration is 10 μg/ml of anti-S1/RBD and 10 μg/ml of anti-N antibodies. Each data point represents the average ( n = 3) with SEM. ( C ) Correlation between DA-D4 readout for IP-10 with an ELISA performed separately. Samples with a letter designate samples from one individual at different time points, where b occurs later in disease than a. All samples were tested in duplicate on the DA-D4 (with SD shown) except 2b (due to insufficient volume). The solid line shows linear regression. ( D ) Antibody reactivity against S1, RBD, and N for sample tested in (C) (with SD shown). NC, negative control pooled healthy plasma.

    Article Snippet: Capture spots of the following proteins were printed as ~170-μm-diameter spots using a Scienion S11 sciFLEXARRAYER (Scienion AG) inkjet printer: spike S1 (Sino Biological, catalog #40591-V05H1), spike RBD (Sino Biological, catalog #40592-V02H), and nucleocapsid protein (Leinco, catalog #S854).

    Techniques: Biomarker Assay, Recombinant, Concentration Assay, Enzyme-linked Immunosorbent Assay, Negative Control