sars cov 2 2019 ncov spike rbd mfc recombinant protein hplc verified covid 19 spike rbd research  (Sino Biological)


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    Name:
    SARS CoV 2 2019 nCoV Spike RBD mFc Recombinant Protein HPLC verified COVID 19 Spike RBD Research
    Description:
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 Arg319 Phe541 was expressed with the Fc region of mouse IgG1 at the C terminus
    Catalog Number:
    40592-V05H
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus spike Protein 2019-nCoV, cov spike Protein 2019-nCoV, ncov RBD Protein 2019-nCoV, ncov s1 Protein 2019-nCoV, ncov s2 Protein 2019-nCoV, ncov spike Protein 2019-nCoV, NCP-CoV RBD Protein 2019-nCoV, NCP-CoV s1 Protein 2019-nCoV, NCP-CoV s2 Protein 2019-nCoV, NCP-CoV Spike Protein 2019-nCoV, novel coronavirus RBD Protein 2019-nCoV, novel coronavirus s1 Protein 2019-nCoV, novel coronavirus s2 Protein 2019-nCoV, novel coronavirus spike Protein 2019-nCoV, RBD Protein 2019-nCoV, S1 Protein 2019-nCoV, S2 Protein 2019-nCoV, Spike RBD Protein 2019-nCoV
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological sars cov 2 2019 ncov spike rbd mfc recombinant protein hplc verified covid 19 spike rbd research
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 Arg319 Phe541 was expressed with the Fc region of mouse IgG1 at the C terminus
    https://www.bioz.com/result/sars cov 2 2019 ncov spike rbd mfc recombinant protein hplc verified covid 19 spike rbd research/product/Sino Biological
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 2019 ncov spike rbd mfc recombinant protein hplc verified covid 19 spike rbd research - by Bioz Stars, 2021-06
    97/100 stars

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    Related Articles

    Recombinant:

    Article Title: A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes
    Article Snippet: Antibody binding and competition with receptor ACE2The binding affinity of antibodies to S protein was analyzed by ELISA. .. The 384-well plate (Corning, Catalog #3700) was coated overnight at 4 °C with PBS containing 30 μL 20 nM of the SARS-CoV-2 Spike S1+S2 ECD, his Tag protein (Sino Biological, Catalog #40589-V08B1), or SARS-CoV-2 Spike RBD-mFc recombinant protein (Sino Biological, Catalog #40592-V05H) or S1-mFc (Sino Biological, Catalog #40591-V05H1). ..

    Article Title: Structure and function analysis of a potent human neutralizing antibody CA521FALA against SARS-CoV-2
    Article Snippet: Mice were bred and kept under specific-pathogen-free conditions. .. Three mice (named Q1–Q3) were immunized in 10-day intervals with the mixture of recombinant RBD protein (Cat 40592-V05H, Sino Biological), Spike S1 protein (Cat 40591-V02H, Sino Biological) and Spike S2 protein (Cat 40590-V08B, Sino Biological) and boosted by 105 μg mixtures (35 μg for each protein) after three rounds immunization. .. Two mice (named Q6 and Q7) were immunized in 10-day intervals with recombinant Spike S1 + S2 (Cat 40589-V08B1, Sino Biological) and boosted by 40 μg recombinant Spike S1 + S2 after 3 rounds of immunization.

    Immunofluorescence:

    Article Title: A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in VeroE6 cells
    Article Snippet: .. Primary antibodies for immunofluorescence studiesanti-S IgG rabbit monoclonal antibody (40592-V05H, Sino Biological), dilution: 1:200. anti-N IgG rabbit monoclonal antibody (40143-R019, Sino Biological), dilution: 1:200. anti-ACE2 IgG rabbit monoclonal antibody (ab15348, AbCam), dilution: 1:200. anti-clathrin IgG mouse monoclonal antibody (sc-12734, SantaCruz), dilution: 1:200. anti-caveolin-1 IgG mouse monoclonal antibody (sc-70516, SantaCruz), dilution: 1:200. anti-CD71 IgG mouse monoclonal antibody (sc-65882, SantaCruz), dilution: 1:100. anti-α-tubulin IgG mouse monoclonal antibody (T5168, Merck), dilution: 1:1000. .. Secondary antibodies for immunofluorescence studies and combinationsdonkey anti-rabbit IgG Alexa488-labeled monoclonal antibody (a21206, ThermoFisher), dilution: 1:500 (confocal, airyscan, and dSTORM-TIRF experiments). donkey anti-rabbit IgG Alexa647-labeled monoclonal antibody (a31573, ThermoFisher), dilution: 1:500 (confocal, airyscan, and dSTORM-TIRF experiments). donkey anti-mouse IgG Alexa488-labeled monoclonal antibody (a21202, ThermoFisher), dilution: 1:500 (confocal, airyscan, and dSTORM-TIRF experiments). donkey anti-mouse IgG Alexa647-labeled monoclonal antibody (a31571, ThermoFisher), dilution: 1:500 (confocal, airyscan, and dSTORM-TIRF experiments). goat anti-rabbit IgG Atto647N-labeled monoclonal antibody (40839, Merck), dilution: 1:200 (τ-STED experiments). goat anti-mouse IgG Atto594-labeled monoclonal antibody (76085, Merck), dilution: 1:200 (τ-STED experiments).

    Mouse Assay:

    Article Title: Structure and function analysis of a potent human neutralizing antibody CA521FALA against SARS-CoV-2
    Article Snippet: Mice were bred and kept under specific-pathogen-free conditions. .. Three mice (named Q1–Q3) were immunized in 10-day intervals with the mixture of recombinant RBD protein (Cat 40592-V05H, Sino Biological), Spike S1 protein (Cat 40591-V02H, Sino Biological) and Spike S2 protein (Cat 40590-V08B, Sino Biological) and boosted by 105 μg mixtures (35 μg for each protein) after three rounds immunization. .. Two mice (named Q6 and Q7) were immunized in 10-day intervals with recombinant Spike S1 + S2 (Cat 40589-V08B1, Sino Biological) and boosted by 40 μg recombinant Spike S1 + S2 after 3 rounds of immunization.

    Article Title: Structure and function analysis of a potent human neutralizing antibody CA521FALA against SARS-CoV-2
    Article Snippet: Two mice (named Q6 and Q7) were immunized in 10-day intervals with recombinant Spike S1 + S2 (Cat 40589-V08B1, Sino Biological) and boosted by 40 μg recombinant Spike S1 + S2 after 3 rounds of immunization. .. Another two mice (named Q14, Q15) were immunized in 10-day intervals with mixtures of Spike S1 protein (Cat 40591-V02H, Sino Biological) and RBD protein (Cat 40592-V05H, Sino Biological) and boosted by 70 μg mixtures (35 μg for each protein) after three rounds immunization. .. Genes of these proteins were derived from the SARS-CoV-2 strain (Wuhan-1 strain, GenBank: MN_908947).

    Article Title: Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells
    Article Snippet: Transposon vectors (SBI System Biosciences, PB514B-2) containing the hACE2 gene and transposase plasmids were co-transfected into BHK21 cells and then selected through puromycin resistance and red fluorescence. .. SARS-CoV-2 spike-specific antibody was generated through the immunization of Balb/c mice with SARS-CoV-2 spike protein (RBD, Fc Tag, 40592-V05H, Sino Biological Inc.). .. MAbs against the SARS-CoV-2 RBD were produced with hybridoma technology and were characterized by pseudotyped virus assay.

    Binding Assay:

    Article Title: Single-cell sequencing of plasma cells from COVID-19 patients reveals highly expanded clonal lineages produce specific and neutralizing antibodies to SARS-CoV-2
    Article Snippet: Enriched cells were then selected for antigen binding by incubating with SARS-CoV-2 S1-mFc 1:75 (Sinobiological, Cat: 40591-V05H1), S2-mFc 1:75 (Sinobiological, Cat: 40590-V05B) on ice for 20 min, followed by a single wash and a secondary staining with anti-mFc IgG1: PE and/or APC [clone RMG1-1] at 1:100 (Biolegend, Cat: 406608/406610). .. After enrichment for S1, cells were also tested for binding to SARS-CoV-2 RBD-mFc 1:375 (Sinobiological, Cat: 40592-V05H), and the same secondary staining. .. FACS was analyzed using FlowJo X software.

    Staining:

    Article Title: Single-cell sequencing of plasma cells from COVID-19 patients reveals highly expanded clonal lineages produce specific and neutralizing antibodies to SARS-CoV-2
    Article Snippet: Enriched cells were then selected for antigen binding by incubating with SARS-CoV-2 S1-mFc 1:75 (Sinobiological, Cat: 40591-V05H1), S2-mFc 1:75 (Sinobiological, Cat: 40590-V05B) on ice for 20 min, followed by a single wash and a secondary staining with anti-mFc IgG1: PE and/or APC [clone RMG1-1] at 1:100 (Biolegend, Cat: 406608/406610). .. After enrichment for S1, cells were also tested for binding to SARS-CoV-2 RBD-mFc 1:375 (Sinobiological, Cat: 40592-V05H), and the same secondary staining. .. FACS was analyzed using FlowJo X software.

    Generated:

    Article Title: Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells
    Article Snippet: Transposon vectors (SBI System Biosciences, PB514B-2) containing the hACE2 gene and transposase plasmids were co-transfected into BHK21 cells and then selected through puromycin resistance and red fluorescence. .. SARS-CoV-2 spike-specific antibody was generated through the immunization of Balb/c mice with SARS-CoV-2 spike protein (RBD, Fc Tag, 40592-V05H, Sino Biological Inc.). .. MAbs against the SARS-CoV-2 RBD were produced with hybridoma technology and were characterized by pseudotyped virus assay.

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    Sino Biological sars cov 2 2019 ncov spike rbd mfc recombinant protein hplc verified covid 19 spike rbd research
    Sars Cov 2 2019 Ncov Spike Rbd Mfc Recombinant Protein Hplc Verified Covid 19 Spike Rbd Research, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 2019 ncov spike rbd mfc recombinant protein hplc verified covid 19 spike rbd research/product/Sino Biological
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 2019 ncov spike rbd mfc recombinant protein hplc verified covid 19 spike rbd research - by Bioz Stars, 2021-06
    97/100 stars
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