baculovirus expressed sars cov 2 spike s1 his  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    SARS CoV 2 2019 nCoV Spike S1 His Recombinant Protein Biotinylated
    Description:
    A DNA sequence encoding the SARS CoV 2 2019 nCoV spike protein S1 Subunit YP 009724390 1 Val16 Arg685 was expressed with a polyhistidine tag at the C terminus The purified protein was biotinylated in vitro
    Catalog Number:
    40591-V08H-B
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus spike Protein 2019-nCoV, cov spike Protein 2019-nCoV, ncov RBD Protein 2019-nCoV, ncov s1 Protein 2019-nCoV, ncov s2 Protein 2019-nCoV, ncov spike Protein 2019-nCoV, NCP-CoV RBD Protein 2019-nCoV, NCP-CoV s1 Protein 2019-nCoV, NCP-CoV s2 Protein 2019-nCoV, NCP-CoV Spike Protein 2019-nCoV, novel coronavirus RBD Protein 2019-nCoV, novel coronavirus s1 Protein 2019-nCoV, novel coronavirus s2 Protein 2019-nCoV, novel coronavirus spike Protein 2019-nCoV, RBD Protein 2019-nCoV, S1 Protein 2019-nCoV, S2 Protein 2019-nCoV, Spike RBD Protein 2019-nCoV
    Host:
    HEK293 Cells
    Buy from Supplier


    Structured Review

    Sino Biological baculovirus expressed sars cov 2 spike s1 his
    A DNA sequence encoding the SARS CoV 2 2019 nCoV spike protein S1 Subunit YP 009724390 1 Val16 Arg685 was expressed with a polyhistidine tag at the C terminus The purified protein was biotinylated in vitro
    https://www.bioz.com/result/baculovirus expressed sars cov 2 spike s1 his/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    baculovirus expressed sars cov 2 spike s1 his - by Bioz Stars, 2021-04
    94/100 stars

    Images

    Related Articles

    Concentration Assay:

    Article Title: Factors of Severity in Patients with COVID-19: Cytokine/Chemokine Concentrations, Viral Load, and Antibody Responses
    Article Snippet: We measured the levels of human anti–SARS-CoV-2 IgG and IgM by using laboratory-developed ELISA. .. SARS-CoV-2 S1-His protein (Sino Biological, Beijing, China) was coated onto 96-well plates (MaxiSorp, Thermo Fisher Scientific, Waltham, MA) at a concentration of 2 μg/mL in PBS. .. Plasma samples were used at dilutions of 1:100, 1:1,000, and 1:10,000. horseradish peroxidase-conjugated antihuman IgG (Jackson Immunoresearch, West Grove, PA) and IgM (MilliporeSigma, Burlington, MA) were used as secondary antibodies.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19
    Article Snippet: Saliva S1 protein ELISA Saliva pooled under the tongue was drooled into a 50 mL tube and stored at −80 °C until analyzed. .. Immuno MaxiSorp 96-well ELISA plates (Thermo Fisher Scientific) were coated overnight at 4 °C with 2 µg/mL recombinant SARS-CoV-2/2019-nCoV S1 protein (Sino Biologicals) diluted in PBS. .. Wells were blocked with 10% skim milk in PBST (PBS + 0.1% Tween 20) at room temperature for 1 h. Two-fold serial dilutions of saliva samples in PBST were transferred to the ELISA plates (in duplicate) and incubated at room temperature for 1 h. Saliva from an asymptomatic individual confirmed negative for SARS-CoV-2 by clinical testing was used as a negative control.

    Recombinant:

    Article Title: Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19
    Article Snippet: Saliva S1 protein ELISA Saliva pooled under the tongue was drooled into a 50 mL tube and stored at −80 °C until analyzed. .. Immuno MaxiSorp 96-well ELISA plates (Thermo Fisher Scientific) were coated overnight at 4 °C with 2 µg/mL recombinant SARS-CoV-2/2019-nCoV S1 protein (Sino Biologicals) diluted in PBS. .. Wells were blocked with 10% skim milk in PBST (PBS + 0.1% Tween 20) at room temperature for 1 h. Two-fold serial dilutions of saliva samples in PBST were transferred to the ELISA plates (in duplicate) and incubated at room temperature for 1 h. Saliva from an asymptomatic individual confirmed negative for SARS-CoV-2 by clinical testing was used as a negative control.

    Article Title: Identification of Human Single-Domain Antibodies against SARS-CoV-2
    Article Snippet: Biolayer Interferometry (BLI) Binding Assays BLI was carried out on an OctetRED96 device (Pall FortéBio) and assays were carried out in 96-well black plates. .. For the binding kinetics of single-domain antibodies with SARS-CoV-2 S1, the recombinant S1-His protein (Sinobiological Inc) at 15 μg/mL buffered in sodium acetate (pH 5.0) was immobilized onto activated AR2G biosensors (Pall FortéBio) and incubated with threefold serial dilutions of single-domain antibodies in kinetics buffer (PBS buffer supplemented with 0.02% Tween 20). .. The experiments included the following steps at 37°C: (1) equilibration (60 s); (2) activation of AR2G by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride/N-hydroxysucci-nimide (300 s); (3) immobilization of S1 protein onto sensors (100 s); (4) quenching with ethanolamine (300 s); (5) baseline in kinetics buffer (120 s); (6) association of antibodies for measurement of k on (300-600 s); and (7) dissociation of antibodies for measurement of k off (300-600 s).

    Binding Assay:

    Article Title: Identification of Human Single-Domain Antibodies against SARS-CoV-2
    Article Snippet: Biolayer Interferometry (BLI) Binding Assays BLI was carried out on an OctetRED96 device (Pall FortéBio) and assays were carried out in 96-well black plates. .. For the binding kinetics of single-domain antibodies with SARS-CoV-2 S1, the recombinant S1-His protein (Sinobiological Inc) at 15 μg/mL buffered in sodium acetate (pH 5.0) was immobilized onto activated AR2G biosensors (Pall FortéBio) and incubated with threefold serial dilutions of single-domain antibodies in kinetics buffer (PBS buffer supplemented with 0.02% Tween 20). .. The experiments included the following steps at 37°C: (1) equilibration (60 s); (2) activation of AR2G by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride/N-hydroxysucci-nimide (300 s); (3) immobilization of S1 protein onto sensors (100 s); (4) quenching with ethanolamine (300 s); (5) baseline in kinetics buffer (120 s); (6) association of antibodies for measurement of k on (300-600 s); and (7) dissociation of antibodies for measurement of k off (300-600 s).

    Incubation:

    Article Title: Identification of Human Single-Domain Antibodies against SARS-CoV-2
    Article Snippet: Biolayer Interferometry (BLI) Binding Assays BLI was carried out on an OctetRED96 device (Pall FortéBio) and assays were carried out in 96-well black plates. .. For the binding kinetics of single-domain antibodies with SARS-CoV-2 S1, the recombinant S1-His protein (Sinobiological Inc) at 15 μg/mL buffered in sodium acetate (pH 5.0) was immobilized onto activated AR2G biosensors (Pall FortéBio) and incubated with threefold serial dilutions of single-domain antibodies in kinetics buffer (PBS buffer supplemented with 0.02% Tween 20). .. The experiments included the following steps at 37°C: (1) equilibration (60 s); (2) activation of AR2G by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride/N-hydroxysucci-nimide (300 s); (3) immobilization of S1 protein onto sensors (100 s); (4) quenching with ethanolamine (300 s); (5) baseline in kinetics buffer (120 s); (6) association of antibodies for measurement of k on (300-600 s); and (7) dissociation of antibodies for measurement of k off (300-600 s).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Sino Biological baculovirus expressed sars cov 2 spike s1 his
    Baculovirus Expressed Sars Cov 2 Spike S1 His, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/baculovirus expressed sars cov 2 spike s1 his/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    baculovirus expressed sars cov 2 spike s1 his - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    Image Search Results