d614g s1  (Sino Biological)


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    Name:
    SARS CoV 2 2019 nCoV Spike S1 D614G ECD Fc Recombinant Protein
    Description:
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike S1 D614G Fc Recombinant Protein YP 009724390 1 Val16 Arg685 D614G was expressed with the Fc region of human IgG1 at the C terminus
    Catalog Number:
    40591-V02H3
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus spike Protein 2019-nCoV, cov spike Protein 2019-nCoV, ncov RBD Protein 2019-nCoV, ncov s1 Protein 2019-nCoV, ncov s2 Protein 2019-nCoV, ncov spike Protein 2019-nCoV, NCP-CoV RBD Protein 2019-nCoV, NCP-CoV s1 Protein 2019-nCoV, NCP-CoV s2 Protein 2019-nCoV, NCP-CoV Spike Protein 2019-nCoV, novel coronavirus RBD Protein 2019-nCoV, novel coronavirus s1 Protein 2019-nCoV, novel coronavirus s2 Protein 2019-nCoV, novel coronavirus spike Protein 2019-nCoV, RBD Protein 2019-nCoV, S1 Protein 2019-nCoV, S2 Protein 2019-nCoV, Spike RBD Protein 2019-nCoV
    Host:
    HEK293 Cells
    		Buy from Supplier

    Structured Review

    Sino Biological d614g s1
    Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) <t>D614G-S1</t> binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike S1 D614G Fc Recombinant Protein YP 009724390 1 Val16 Arg685 D614G was expressed with the Fc region of human IgG1 at the C terminus
    https://www.bioz.com/result/d614g s1/product/Sino Biological
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d614g s1 - by Bioz Stars, 2021-05
    97/100 stars

    Images

    1) Product Images from "Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection"

    Article Title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection

    Journal: ACS Nano

    doi: 10.1021/acsnano.0c06836

    Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.
    Figure Legend Snippet: Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.

    Techniques Used: Binding Assay, Western Blot, Immunofluorescence, Microscopy, Staining

    2) Product Images from "Membrane Nanoparticles Derived from ACE2-rich Cells Block SARS-CoV-2 Infection"

    Article Title: Membrane Nanoparticles Derived from ACE2-rich Cells Block SARS-CoV-2 Infection

    Journal: bioRxiv

    doi: 10.1101/2020.08.12.247338

    Inhibition of HEK-293T-hACE2 NPs on SARS-CoV-2 D614G-S1. (a) Binding kinetics for ACE2 and D614G-S1 loaded on SA biosensors. The fitting curves are in red. (b) Binding kinetics for increasing concentrations of HEK-293T-hACE2 NPs and D614G-S1 loaded on SA biosensors. (c) Immunofluorescence microscopy revealing the protection of HEK-293T-hACE2 NPs on HK-2 cells exposed to D614G-S1 (green). Nuclei are stained using DAPI (blue). Scale bar indicates 20 μm.
    Figure Legend Snippet: Inhibition of HEK-293T-hACE2 NPs on SARS-CoV-2 D614G-S1. (a) Binding kinetics for ACE2 and D614G-S1 loaded on SA biosensors. The fitting curves are in red. (b) Binding kinetics for increasing concentrations of HEK-293T-hACE2 NPs and D614G-S1 loaded on SA biosensors. (c) Immunofluorescence microscopy revealing the protection of HEK-293T-hACE2 NPs on HK-2 cells exposed to D614G-S1 (green). Nuclei are stained using DAPI (blue). Scale bar indicates 20 μm.

    Techniques Used: Inhibition, Binding Assay, Immunofluorescence, Microscopy, Staining

    3) Product Images from "Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection"

    Article Title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection

    Journal: ACS Nano

    doi: 10.1021/acsnano.0c06836

    Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.
    Figure Legend Snippet: Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.

    Techniques Used: Binding Assay, Western Blot, Immunofluorescence, Microscopy, Staining

    4) Product Images from "Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection"

    Article Title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection

    Journal: ACS Nano

    doi: 10.1021/acsnano.0c06836

    Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.
    Figure Legend Snippet: Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.

    Techniques Used: Binding Assay, Western Blot, Immunofluorescence, Microscopy, Staining

    5) Product Images from "Membrane Nanoparticles Derived from ACE2-rich Cells Block SARS-CoV-2 Infection"

    Article Title: Membrane Nanoparticles Derived from ACE2-rich Cells Block SARS-CoV-2 Infection

    Journal: bioRxiv

    doi: 10.1101/2020.08.12.247338

    Inhibition of HEK-293T-hACE2 NPs on SARS-CoV-2 D614G-S1. (a) Binding kinetics for ACE2 and D614G-S1 loaded on SA biosensors. The fitting curves are in red. (b) Binding kinetics for increasing concentrations of HEK-293T-hACE2 NPs and D614G-S1 loaded on SA biosensors. (c) Immunofluorescence microscopy revealing the protection of HEK-293T-hACE2 NPs on HK-2 cells exposed to D614G-S1 (green). Nuclei are stained using DAPI (blue). Scale bar indicates 20 μm.
    Figure Legend Snippet: Inhibition of HEK-293T-hACE2 NPs on SARS-CoV-2 D614G-S1. (a) Binding kinetics for ACE2 and D614G-S1 loaded on SA biosensors. The fitting curves are in red. (b) Binding kinetics for increasing concentrations of HEK-293T-hACE2 NPs and D614G-S1 loaded on SA biosensors. (c) Immunofluorescence microscopy revealing the protection of HEK-293T-hACE2 NPs on HK-2 cells exposed to D614G-S1 (green). Nuclei are stained using DAPI (blue). Scale bar indicates 20 μm.

    Techniques Used: Inhibition, Binding Assay, Immunofluorescence, Microscopy, Staining

    6) Product Images from "Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection"

    Article Title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection

    Journal: ACS Nano

    doi: 10.1021/acsnano.0c06836

    Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.
    Figure Legend Snippet: Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.

    Techniques Used: Binding Assay, Western Blot, Immunofluorescence, Microscopy, Staining

    Related Articles

    other:

    Article Title: Membrane Nanoparticles Derived from ACE2-rich Cells Block SARS-CoV-2 Infection
    Article Snippet: Biotinylated SARS-CoV-2 RBD and D614G-S1 were obtained with a G-MM-IGT biotinylation kit (Genemore, Shanghai, CHN) was immobilized on SA biosensors at 15 μg mL−1 .

    Article Title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection
    Article Snippet: To determine the amounts of S1 and D614G-S1 recruited to HK-2 cells, cells were seeded into a 6-well plate at a density of 1 × 106 cells per well.

    Article Title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection
    Article Snippet: Biotinylated RBD and D614G-S1 were obtained with a G-MM-IGT biotinylation kit (Genemore, Shanghai, CHN) and immobilized on SA biosensors at 15 μg mL–1 .

    Article Title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection
    Article Snippet: In the sham group, immobilized RBD and D614G-S1 were immersed in PBS.

    Article Title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection
    Article Snippet: Association was performed with shaking at 1,000 rpm for 300 s. To determine the affinity of ACE2 for D614G-S1, ACE2 was prepared in PBS at concentrations of 100, 75, 50, 25, and 10 nM.

    Purification:

    Article Title: BNT162b vaccines are immunogenic and protect non-human primates against SARS-CoV-2
    Article Snippet: A recombinant SARS-CoV-2 RBD containing a C-terminal Avitag™ (Acro Biosystems) was used as a target antigen in Luminex immunoassays. .. Purified recombinant SARS-CoV-2 S1 including a histidine tag (Sino Biological) was used in ELISA to detect SARS-CoV-2 S-specific IgG in mice. .. Purified recombinant SARS-CoV-2 S1 and RBD with histidine tags (both Sino Biological) were used for surface plasmon resonance (SPR) spectroscopy.

    Recombinant:

    Article Title: BNT162b vaccines are immunogenic and protect non-human primates against SARS-CoV-2
    Article Snippet: A recombinant SARS-CoV-2 RBD containing a C-terminal Avitag™ (Acro Biosystems) was used as a target antigen in Luminex immunoassays. .. Purified recombinant SARS-CoV-2 S1 including a histidine tag (Sino Biological) was used in ELISA to detect SARS-CoV-2 S-specific IgG in mice. .. Purified recombinant SARS-CoV-2 S1 and RBD with histidine tags (both Sino Biological) were used for surface plasmon resonance (SPR) spectroscopy.

    Article Title: Therapeutic activity of an inhaled potent SARS-CoV-2 neutralizing human monoclonal antibody in hamsters
    Article Snippet: Binding characterizationELISA plates (Nunc MaxiSorp; Thermo Fisher Scientific, Grand Island, NY) were coated with recombinant SARS-CoV-2 proteins at 1□μg/ml. .. Recombinant proteins used include SARS-CoV-2 S1+S2 (Sino Biological, Wayne, PA), SARS-CoV-2 D614G S1 (Sino Biological), SARS-CoV-1 S (BEI Resources), SARS-CoV-2 Nucleocapsid (Sino Biological), and HepG2 whole cell lysate (Abcam, Cambridge, MA). .. Purified hmAbs were diluted in PBS, and binding was detected with HRP-conjugated anti-human IgG (Jackson ImmunoResearch, West Grove, PA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: BNT162b vaccines are immunogenic and protect non-human primates against SARS-CoV-2
    Article Snippet: A recombinant SARS-CoV-2 RBD containing a C-terminal Avitag™ (Acro Biosystems) was used as a target antigen in Luminex immunoassays. .. Purified recombinant SARS-CoV-2 S1 including a histidine tag (Sino Biological) was used in ELISA to detect SARS-CoV-2 S-specific IgG in mice. .. Purified recombinant SARS-CoV-2 S1 and RBD with histidine tags (both Sino Biological) were used for surface plasmon resonance (SPR) spectroscopy.

    Mouse Assay:

    Article Title: BNT162b vaccines are immunogenic and protect non-human primates against SARS-CoV-2
    Article Snippet: A recombinant SARS-CoV-2 RBD containing a C-terminal Avitag™ (Acro Biosystems) was used as a target antigen in Luminex immunoassays. .. Purified recombinant SARS-CoV-2 S1 including a histidine tag (Sino Biological) was used in ELISA to detect SARS-CoV-2 S-specific IgG in mice. .. Purified recombinant SARS-CoV-2 S1 and RBD with histidine tags (both Sino Biological) were used for surface plasmon resonance (SPR) spectroscopy.

    Binding Assay:

    Article Title: Membrane Nanoparticles Derived from ACE2-rich Cells Block SARS-CoV-2 Infection
    Article Snippet: .. Association was performed at a shaking speed of 1000 rpm and ran for 300 s. To determine the affinity of ACE2 binding to D614G-S1, ACE2 was prepared in PBS with concentrations of 100, 75, 50, 25, and 10 nM. .. The running times for association and disassociation were both 300 s. The binding data were processed using Fortebio Data Analysis 7.0 software.

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    • d614g s1  (Sino Biological)
    97
    Sino Biological d614g s1
    Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) <t>D614G-S1</t> binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.
    D614g S1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d614g s1/product/Sino Biological
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d614g s1 - by Bioz Stars, 2021-05
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.

    Journal: ACS Nano

    Article Title: Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection

    doi: 10.1021/acsnano.0c06836

    Figure Lengend Snippet: Inhibitory effect of ACE2-NPs on S1 recruitment. (a) Binding kinetics for NPs and SARS-CoV-2 RBD loaded on SA biosensors. (b) Western blotting detection of S1 and (e) D614G-S1 binding to HK-2 in the absence and presence of NPs. β-actin was used as the reference. (c) Immunofluorescence microscopy revealing the protective effect of NPs on cells exposed to S1 (green). The region of interest in the S1-treated group is magnified in the inset graph. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. (d) Binding kinetics for increasing concentrations of ACE2 and D614G-S1 loaded on SA biosensors. The fitted curves are colored red. The fitting coefficient ( R 2 ) is 0.96.

    Article Snippet: In the sham group, immobilized RBD and D614G-S1 were immersed in PBS.

    Techniques: Binding Assay, Western Blot, Immunofluorescence, Microscopy, Staining

    Inhibition of HEK-293T-hACE2 NPs on SARS-CoV-2 D614G-S1. (a) Binding kinetics for ACE2 and D614G-S1 loaded on SA biosensors. The fitting curves are in red. (b) Binding kinetics for increasing concentrations of HEK-293T-hACE2 NPs and D614G-S1 loaded on SA biosensors. (c) Immunofluorescence microscopy revealing the protection of HEK-293T-hACE2 NPs on HK-2 cells exposed to D614G-S1 (green). Nuclei are stained using DAPI (blue). Scale bar indicates 20 μm.

    Journal: bioRxiv

    Article Title: Membrane Nanoparticles Derived from ACE2-rich Cells Block SARS-CoV-2 Infection

    doi: 10.1101/2020.08.12.247338

    Figure Lengend Snippet: Inhibition of HEK-293T-hACE2 NPs on SARS-CoV-2 D614G-S1. (a) Binding kinetics for ACE2 and D614G-S1 loaded on SA biosensors. The fitting curves are in red. (b) Binding kinetics for increasing concentrations of HEK-293T-hACE2 NPs and D614G-S1 loaded on SA biosensors. (c) Immunofluorescence microscopy revealing the protection of HEK-293T-hACE2 NPs on HK-2 cells exposed to D614G-S1 (green). Nuclei are stained using DAPI (blue). Scale bar indicates 20 μm.

    Article Snippet: Association was performed at a shaking speed of 1000 rpm and ran for 300 s. To determine the affinity of ACE2 binding to D614G-S1, ACE2 was prepared in PBS with concentrations of 100, 75, 50, 25, and 10 nM.

    Techniques: Inhibition, Binding Assay, Immunofluorescence, Microscopy, Staining