sars cov 2 2019 ncov spike s2 ecd fc recombinant protein  (Sino Biological)


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    Name:
    SARS CoV 2 2019 nCoV Spike S2 ECD Fc Recombinant Protein
    Description:
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein S2 ECD YP 009724390 1 Ser686 Pro1213 was expressed with the Fc region of human IgG1 at the C terminus
    Catalog Number:
    40590-V02H
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus spike Protein 2019-nCoV, cov spike Protein 2019-nCoV, ncov RBD Protein 2019-nCoV, ncov s1 Protein 2019-nCoV, ncov s2 Protein 2019-nCoV, ncov spike Protein 2019-nCoV, NCP-CoV RBD Protein 2019-nCoV, NCP-CoV s1 Protein 2019-nCoV, NCP-CoV s2 Protein 2019-nCoV, NCP-CoV Spike Protein 2019-nCoV, novel coronavirus RBD Protein 2019-nCoV, novel coronavirus s1 Protein 2019-nCoV, novel coronavirus s2 Protein 2019-nCoV, novel coronavirus spike Protein 2019-nCoV, RBD Protein 2019-nCoV, S1 Protein 2019-nCoV, S2 Protein 2019-nCoV, Spike RBD Protein 2019-nCoV
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological sars cov 2 2019 ncov spike s2 ecd fc recombinant protein
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein S2 ECD YP 009724390 1 Ser686 Pro1213 was expressed with the Fc region of human IgG1 at the C terminus
    https://www.bioz.com/result/sars cov 2 2019 ncov spike s2 ecd fc recombinant protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 2019 ncov spike s2 ecd fc recombinant protein - by Bioz Stars, 2021-04
    94/100 stars

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    Related Articles

    other:

    Article Title: An all-solid-state heterojunction oxide transistor for the rapid detection of biomolecules and SARS-CoV-2 spike S1 protein
    Article Snippet: For the SARS-CoV-2 spike protein sensing, the tri-channel transistors were first treated with UV-irradiation for 10 min, APTES solution (2 wt%) in toluene was pipetted onto the oxide surface and left for 15 min, followed by rinsing with toluene and annealing at 120 °C for 1 h. A glutaraldehyde (GA) linker was added to the terminal amino (-NH2 ) groups of APTES using a solution of 0.8% GA in DI water for 10 min at room temperature, followed by rinsing with DI water and dried with a stream of N2 gas.

    In Vitro:

    Article Title: Tackling the COVID-19 “cytokine storm” with microRNA mimics directly targeting the 3’UTR of pro-inflammatory mRNAs
    Article Snippet: .. The experimental approach that might be employed is described in and it is based on the induction of pro-inflammatory mRNAs following exposure of in vitro growing cell lines to the SARS-CoV-2 Spike protein. .. This treatment leads to fast and reproducible increase of the expression of pro-inflammatory mRNA, as reported by Wang et al. who reported an upregulation of IL-6 and TNF-alpha induced by SARS-coronavirus spike protein in murine macrophages via NF-kB pathway.

    Stable Transfection:

    Article Title: High-throughput detection of antibodies targeting the SARS-CoV-2 Spike in longitudinal convalescent plasma samples
    Article Snippet: 293T cells were transfected with 10 μg of Spike expressor and 2 μg of a green fluorescent protein (GFP) expressor (pIRES-GFP) for 2×106 293T cells using the standard calcium phosphate method. .. For the generation of 293T cells stably expressing the SARS-CoV-2 Spike protein, transgenic lentiviruses were produced in 293T using a third-generation lentiviral vector system. .. Briefly, 293T cells were co-transfected with two packaging plasmids (pLP1 and pLP2), an envelope plasmid (pSVCMV-IN-VSV-G) and a lentiviral transfer plasmid coding for a GFP-tagged SARS-CoV-2 Spike (pLV-SARS-CoV-2 S C-GFPSpark tag) (Sino Biological).

    Expressing:

    Article Title: High-throughput detection of antibodies targeting the SARS-CoV-2 Spike in longitudinal convalescent plasma samples
    Article Snippet: 293T cells were transfected with 10 μg of Spike expressor and 2 μg of a green fluorescent protein (GFP) expressor (pIRES-GFP) for 2×106 293T cells using the standard calcium phosphate method. .. For the generation of 293T cells stably expressing the SARS-CoV-2 Spike protein, transgenic lentiviruses were produced in 293T using a third-generation lentiviral vector system. .. Briefly, 293T cells were co-transfected with two packaging plasmids (pLP1 and pLP2), an envelope plasmid (pSVCMV-IN-VSV-G) and a lentiviral transfer plasmid coding for a GFP-tagged SARS-CoV-2 Spike (pLV-SARS-CoV-2 S C-GFPSpark tag) (Sino Biological).

    Transgenic Assay:

    Article Title: High-throughput detection of antibodies targeting the SARS-CoV-2 Spike in longitudinal convalescent plasma samples
    Article Snippet: 293T cells were transfected with 10 μg of Spike expressor and 2 μg of a green fluorescent protein (GFP) expressor (pIRES-GFP) for 2×106 293T cells using the standard calcium phosphate method. .. For the generation of 293T cells stably expressing the SARS-CoV-2 Spike protein, transgenic lentiviruses were produced in 293T using a third-generation lentiviral vector system. .. Briefly, 293T cells were co-transfected with two packaging plasmids (pLP1 and pLP2), an envelope plasmid (pSVCMV-IN-VSV-G) and a lentiviral transfer plasmid coding for a GFP-tagged SARS-CoV-2 Spike (pLV-SARS-CoV-2 S C-GFPSpark tag) (Sino Biological).

    Produced:

    Article Title: High-throughput detection of antibodies targeting the SARS-CoV-2 Spike in longitudinal convalescent plasma samples
    Article Snippet: 293T cells were transfected with 10 μg of Spike expressor and 2 μg of a green fluorescent protein (GFP) expressor (pIRES-GFP) for 2×106 293T cells using the standard calcium phosphate method. .. For the generation of 293T cells stably expressing the SARS-CoV-2 Spike protein, transgenic lentiviruses were produced in 293T using a third-generation lentiviral vector system. .. Briefly, 293T cells were co-transfected with two packaging plasmids (pLP1 and pLP2), an envelope plasmid (pSVCMV-IN-VSV-G) and a lentiviral transfer plasmid coding for a GFP-tagged SARS-CoV-2 Spike (pLV-SARS-CoV-2 S C-GFPSpark tag) (Sino Biological).

    Plasmid Preparation:

    Article Title: High-throughput detection of antibodies targeting the SARS-CoV-2 Spike in longitudinal convalescent plasma samples
    Article Snippet: 293T cells were transfected with 10 μg of Spike expressor and 2 μg of a green fluorescent protein (GFP) expressor (pIRES-GFP) for 2×106 293T cells using the standard calcium phosphate method. .. For the generation of 293T cells stably expressing the SARS-CoV-2 Spike protein, transgenic lentiviruses were produced in 293T using a third-generation lentiviral vector system. .. Briefly, 293T cells were co-transfected with two packaging plasmids (pLP1 and pLP2), an envelope plasmid (pSVCMV-IN-VSV-G) and a lentiviral transfer plasmid coding for a GFP-tagged SARS-CoV-2 Spike (pLV-SARS-CoV-2 S C-GFPSpark tag) (Sino Biological).

    Incubation:

    Article Title: Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins
    Article Snippet: To prepare the AuNPs with specific antibodies [SARS-CoV-2 (2019-nCoV) Spike S1-His recombinant protein (Sino Biological Inc.)], we used both lyophilized 99% bovine serum albumin (BSA) (Sigma-Aldrich) and pH~7.4 phosphate buffer solution (PBS) to dissolve the immunoreagents. .. For real-time characterization, after washing the chips with PBS, antibody-modified structures were incubated in PBS containing 0.1 wt % BSA for 30 min. Next, different concentrations of SARS-CoV-2 recombinant spike protein ranging from 2 to 50 fM in PBS was prepared using serial dilution. ..

    Recombinant:

    Article Title: Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins
    Article Snippet: To prepare the AuNPs with specific antibodies [SARS-CoV-2 (2019-nCoV) Spike S1-His recombinant protein (Sino Biological Inc.)], we used both lyophilized 99% bovine serum albumin (BSA) (Sigma-Aldrich) and pH~7.4 phosphate buffer solution (PBS) to dissolve the immunoreagents. .. For real-time characterization, after washing the chips with PBS, antibody-modified structures were incubated in PBS containing 0.1 wt % BSA for 30 min. Next, different concentrations of SARS-CoV-2 recombinant spike protein ranging from 2 to 50 fM in PBS was prepared using serial dilution. ..

    Serial Dilution:

    Article Title: Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins
    Article Snippet: To prepare the AuNPs with specific antibodies [SARS-CoV-2 (2019-nCoV) Spike S1-His recombinant protein (Sino Biological Inc.)], we used both lyophilized 99% bovine serum albumin (BSA) (Sigma-Aldrich) and pH~7.4 phosphate buffer solution (PBS) to dissolve the immunoreagents. .. For real-time characterization, after washing the chips with PBS, antibody-modified structures were incubated in PBS containing 0.1 wt % BSA for 30 min. Next, different concentrations of SARS-CoV-2 recombinant spike protein ranging from 2 to 50 fM in PBS was prepared using serial dilution. ..

    Immunohistochemistry:

    Article Title: Neuropathology of patients with COVID-19 in Germany: a post-mortem case series
    Article Snippet: To normalise for input, quantitative β-globin PCR was done with a commercial TaqMan primer kit (catalogue number Hs00758889_s1; Thermo Fisher Scientific, Waltham, MA, USA), and the amount of DNA was normalised with use of a KAPA Human Genomic DNA Quantification and QC DNA Standard (catalogue number 07960638001; KAPA Biosystems, Cape Town, South Africa). .. Immunohistochemical detection of SARS-CoV-2 spike protein and nucleoproteinAntibodies for detecting SARS-CoV-2 in FFPE tissues were first validated on SARS-CoV-2-infected (Hamburg isolate) and non-infected Vero cells that were processed to FFPE blocks ( ). .. In specimens with sufficient high-quality tissue, we tested for the presence of the virus with immunohistochemistry using antibodies against viral nucleocapsid protein (catalogue numbers 40143-R001 [dilution 1:5000] and 40143-T62 [dilution 1:1000]; Sino Biological, Eschborn, Germany) and spike protein (clone 1A9, catalogue number GTX632604; GeneTex, Irvine, USA; dilution 1:300).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Neuropathology of patients with COVID-19 in Germany: a post-mortem case series
    Article Snippet: To normalise for input, quantitative β-globin PCR was done with a commercial TaqMan primer kit (catalogue number Hs00758889_s1; Thermo Fisher Scientific, Waltham, MA, USA), and the amount of DNA was normalised with use of a KAPA Human Genomic DNA Quantification and QC DNA Standard (catalogue number 07960638001; KAPA Biosystems, Cape Town, South Africa). .. Immunohistochemical detection of SARS-CoV-2 spike protein and nucleoproteinAntibodies for detecting SARS-CoV-2 in FFPE tissues were first validated on SARS-CoV-2-infected (Hamburg isolate) and non-infected Vero cells that were processed to FFPE blocks ( ). .. In specimens with sufficient high-quality tissue, we tested for the presence of the virus with immunohistochemistry using antibodies against viral nucleocapsid protein (catalogue numbers 40143-R001 [dilution 1:5000] and 40143-T62 [dilution 1:1000]; Sino Biological, Eschborn, Germany) and spike protein (clone 1A9, catalogue number GTX632604; GeneTex, Irvine, USA; dilution 1:300).

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  • 94
    Sino Biological sars cov 2 2019 ncov spike s2 ecd fc recombinant protein
    Sars Cov 2 2019 Ncov Spike S2 Ecd Fc Recombinant Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 2019 ncov spike s2 ecd fc recombinant protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 2019 ncov spike s2 ecd fc recombinant protein - by Bioz Stars, 2021-04
    94/100 stars
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